Objective:To investigate the protective effects of quercetin ( QU) on myocardial ischemia/reperfusion ( MI/R) injury in rats and the underlying mechanisms. Methods:MI/R injury model in rats was prepared by coronary artery ligation for 30 min fol-lowed by 120 min reperfusion. And then the rats were randomly divided into 5 groups, QU (25, 50 and 100 mg·kg-1 ) groups, the model group and the sham group (n=10). QU was orally administered once a day for 7 days before the operation. The CK and LDH activities and TNF-α and IL-1β levels in serum were examined respectively by colorimetric and ELISA method after the reperfusion. Besides, the area of myocardial infarction was calculated and MPO activity in heart homogenate was detected. Results:QU (100 and 50 mg·kg-1 ) could significantly reduced the area of myocardial infarction to 25. 00% and 25. 31%, respectively, and compared with that of 32. 55% in the model group, the difference was significant (P<0. 05). The CK activity in the three QU groups was 779. 82, 793.90 and 870.86 U·L-1, respectively, which was significantly lower than that (1 123.78 U·L-1) in the model group (P<0. 01). The LDH activity in the three QU groups was 2 138. 21, 2 277. 40 and 2 416. 53 U·L-1, respectively, which was significant-ly lower than that (3 105.32 U·L-1) in the model group (P<0.05 or P<0.01). Compared with that (311.72 U·g-1) in the model group, the MPO activity in heart homogenate in the three QU groups was reduced to 185. 70, 190. 66 and 210. 03 U·g-1 , re-spectively (P<0. 05 or P<0. 01). Compared with that (119. 55 pg·ml-1) in the model group, QU (100 and 50 mg·kg-1) signif-icantly reduced the level of TNF-αto 97. 48 and 97. 54 pg·ml-1, respectively (P<0. 05). Compared with that (673. 34 pg·ml-1) in the model group, IL-1βlevel in serum in the three QU groups was evidently reduced to 419. 26, 438. 72 and 492. 53 pg·ml-1 , re-spectively (P<0. 01). Conclusion:The effectiveness of QU pretreatment in MI/R injury appears to be associated with the inhibition of neutrophil infiltration and expression of inflammatory cytokines.

Objective To study on the application of biological variation total allowable error in quality management of serum lipid detection .Methods The σ score ,quality goal index (QGI) ,priority improvement measures and performance evaluation of lipid accuracy criteria were evaluated ,including cholesterol (CHOL) ,triglyceride (TG) ,high density lipoprotein (HDL‐C) ,low density lipoprotein (LDL‐C) ,which included in the standard of accuracy of the Ministry of Health in 2015 .The reason for unsatisfactory re‐sults of cholesterol (CHOL) test were analyzed .Results Based on the three levels of quality specifications derived in biological var‐iation ,when the total allowable error was located at an appropriate level ,the σ score of TG ,HDL‐C ,LDL‐C reached 6 ,it was not re‐quired for improvement .While the score of CHOL performance analysis was poor ,accuracy was required to give priority to impro‐ving .When the total allowable error reached the best level ,only the σ score of TG achieved “good” in the four items ,improvement of precision was needed .When the total allowable error was located at the lowest level ,the σ score of TG ,HDL‐C ,LDL‐C was grea‐ter than 6 ,the score of performance analysis reached the “excellent” .The σ score of CHOL (2 .9) had been closen to 3σ ,accuracy was required to be corrected .Conclusion The biological variation derived total allowable error is easy to meet the requirements of the quality management in serum lipid determination by current technologies and methods .The theory of 6σ quality can reflect the performance of detection indexes ,and improve the quality of analysis effectively .

Objective:To investigate the effects of paeonol on aortic nuclear factor-kappaB ( NF-κB) activity and blood lipid levels in the rats with atherosclerosis ( AS) . Methods:The AS rat model was established by administration of high-fat diet and vitamin D3 ( i. p. ). The rats were divided into 5 groups (n=8) randomly, namely the normal group, model group, positive control group(simvasta-tin, 10 mg·kg-1 ) and paeonol groups with the dose of 20 and 10 mg·kg-1 , respectively. After the 4-week treatment, the serum of rats was collected to determine the lipid levels. The aortic pathologic changes of AS rats were observed under a microscope, and then graded, and aortic NF-κB activity was detected by an immunohistochemistry method. Results:Administration of paeonol improved the severity of aortic pathology in AS rats. The pathologic grade in the paeonol group (20 mg·kg-1 ) showed a significant difference com-pared with that in the model group (P<0. 05). Paeonol (20 and 10 mg·kg-1) markedly decreased the TG, TC and LDL-C levels in the serum of AS rats (P<0. 05 or P<0. 01). Meanwhile, paeonol significantly inhibited the aortic NF-κB activity(IOD value) with (1. 96 ± 0. 55) and (2. 50 ± 0. 80) in 20 and 10 mg·kg-1 group, respectively, which showed significant differences compared with that in the model group (P<0. 05 or P<0. 01). Conclusion:Paeonol shows certain therapeutic effect in AS rats, and the mechanisms may be related with the regulation of lipid mobilization and the inhibition of aortic NF-κB activity.

Objective To analyze the precision and accuracy of the Olympus AU5421 automatic biochemical analyzer in order to verify the performance of this detection system declared by the manufacturer.Methods The Precision and Accuracy of User Au-thentication-Guide for Approval Second Edition(CLSI EP15-A2)was used to perform the routine detection on the Olympus AU5421 automatic biochemical analyzer.The systematic precision and accuracy were analyzed.Results Under this experiment condition,the precision and accuracy of the Olympus AU5421 automatic biochemical analyzer was consistent with the performance declared by the manufacturer.Conclusion The Olympus AU5421 automatic biochemical analyzer system has the high precision and good accuracy, and can be better applied in the clinical routine detection.

Grid management plays an important role in improving the efficiency of social service management. The authors explored the establishment of a nosocomial infection grid management model and implemented it at a tertiary general hospital in Shanghai. χ2 test results showed that since the hospital fully implemented the grid management model in early 2016, the number of hospital infections, surgical site infections, type I surgical incision infections, and ventilator-related pneumonia had shown a downward trend year by year. The number of inspections before the use of antibacterial drugs, the number of blood cultures submitted for fever patients, and the timing of hand hygiene compliance showed an increasing trend year by year( P<0.05), and the management of nosocomial infection was more effective than the previous period. The application of grid management model required establishment of grid organization structure, control of key links, implementation of the special personnel responsibility, formulation of a performance evaluation system, and implementation of incentive measures. The grid management model can significantly reduce the risk of hospital infection, improve the efficiency of hospital infection management, and was valuable to be applicated and promoted in medical institutions.

Objective:To investigate the anti-rejection effect of the berberine combined with ciclosporin A (CsA) against mice with xenogenic skin transplantation and explore the mechanisms.Methods:The skin transplantation model was established by an operation, tergal skin flaps from BALB/c donor mice were transplanted to the back of C57BL/6 recipient mice. Then recipient mice were randomly divided into 5 groups ( n=20), namely sham (0.5% CMC-Na), model (0.5% CMC-Na), berberine (100 mg/kg), CsA (10 mg/kg), berberine combined with CsA (berberine 100 mg/kg and CsA 5 mg/kg). All recipient mice were intraperitoneally administered once a day for 10 days. The skin graft survival time of all mice were noted everyday. At the end of the experiment, the levels of Th1 cytokines (IL-2 and IFN-γ) and Th2 cytokines (IL-4 and IL-10) in plasma were detected by ELISA, and quantity of CD4 + CD25 + T cells were analysed by flow cytometry. Results:Compared with model group, the survival days of skin graft of those in groups treated with drugs were all significantly prolonged ( P<0.01). Berberine combined with CsA group showed a longer survival days than berberine or CsA group ( P<0.01). Compared to the model group, the content of IL-2 (11.55 ± 3.14 pg/ml vs. 19.85 ± 2.42 pg/ml), IFN-γ (26.41 ± 6.20 pg/ml vs. 57.23 ± 10.15 pg/ml) of berberine combined with ciclosporin A group significantly decreased ( P<0.05). The content of IL-4 (192.45 ± 70.12 pg/ml vs. 61.09 ± 21.61 pg/ml), IL-10 (106.79 ± 27.83 pg/ml vs. 40.08 ± 11.23 pg/ml) of berberine combined with ciclosporin A group significantly increased ( P<0.05). The amount of CD4 + CD25 + T cells (7.65% ± 2.42% vs. 3.69% ± 0.83%) in berberine combined with ciclosporin A group significantly increased ( P<0.01). Conclusions:The immunosuppressive effect of berberine combined with CsA maybe related with induction of immune tolerance, balance of Th1/Th2 cytokines and increasement of CD4 + CD25 + T cells expression.

Objectives To investigate the incidence of brain injuri in premature infants in ten hospitals of seven large cities in China sponsored by the Subspecialty Group of Neonatology of Pediatric Society, China Medical Association. Methods All premature infants with gestational age less than 37 weeks in ten hospitals were given routine cranial ultrasound within three days of birth, and then repeated every 3-7 days till the discharge from the hospital during January 2005 to August 2006. Results Incidence of intraventricular hemorrhage (IVH) and severe IVH were 10.8% (406/3 768) and 2.4% (92/3 768) with 22.6% (92/406) for grade 1, 54.7% (222/406) for grade 2, 17.2% (70/406) for grade 3 and 5.4% (22/406) for grade 4 in nine hospitals; incidence of periventricular leukomalacia (PVL) and cystic PVL were 2.3% (112/4 933) and 0.3% (16/4 933) with 85.7% (96/112) for grade 1, 12.5% (14/112) for grade 2, and 1.8% (2/112) for grade 3 including all ten hospitals, respectively. Risk factors associated with increased severity of IVH were vaginal delivery (OR = 1.874, 95% CI = 1.172 - 2.997, P < 0.01), perinatal asphyxia (OR = 1.598, 95% CI = 1.077 - 2.372, P < 0.05), mechanical ventilation (OR = 3.988, 95% CI= 2.448 -6.948, P< 0.01), and amniotic fluid contamination (OR = 2.192, 95% CI = 1.054 - 4.544, P< 0.05). Risk factors that might result in the development of cystic PVL were vaginal delivery (OR = 1.400, 95% CI = 1.186 - 1.652, P < 0.001) and mechanical ventilation (OR = 3.000, 95% CI = 1.015 - 8.864, P < 0.05). Conclusions These data reflect basically the prevalence of brain injuriy in premature infants in major cities of China. However, more than 60% of population lives in the rural area, further multicenter investigation including the rural area is expected to be undertaken in future.

Objective To analyze the prospective coping styles of nurses in operation room of plastic surgery department and its influencing factors. Methods A total of 169 nurses of two tertiary first-class hospitals and two secondary hospitals in Yuncheng city were selected as the research objects by convenient sampling from January 2016 to January 2017, their general data, dispositional hope, occupational expectations, occupational values, occupational maturity and prospective coping styles were analyzed. The influencing factors of prospective coping style were analyzed. Results The quality of hope was (31.78±4.24) points, occupational expectation was (72.41±7.24) points, professional values were (86.63 ± 7.02) points, professional maturity was (94.58 ± 6.25) points, and prospective coping style was (40.64 ± 6.13) points in the nurses in plastic surgery operation room. The results of multivariate linear regression model showed that the longer working time, high professional title, high degree of education, high monthly income, longer working hours in plastic surgery operation room, high hope quality, high career expectation, high occupational values and high career maturity were all the prospective coping styles of nurses in the operation room. Significant factors (P <0.05). Conclusions The nurses in the plastic surgery operation room have a certain prospective coping style, improve the treatment of nurses, set up their active work goals, improve their hope traits, career expectations, occupational values and career maturity, which can significantly enhance the level of prospective coping style.

Object:To explore the effects of shared decision making-based intervention on decisional conflict and health literacy in stable coronary artery disease patients.Methods:A total of 86 stable coronary artery disease patients were divided into experimental group and control group according to the admission order, with 43 cases in each group. The patients in the control group recieved routine nursing, basis on this, the experimental group carried out shared decision making program. Before and after 1 month of intervention, the effects was assessed by Decisional conflict scale (DCS) and Health literacy management scale (HeLMS), respectively.Results:Before intervention, there was no significant difference in DCS and HeLMS scores between two group( P>0.05). After intervention, the information & values, decision uncertainty and total DCS scores were (6.95±2.13) pionts, (3.44±1.18) pionts and (20.95±3.99) pionts, significantly lower than in the control group (8.77±2.33) pionts, (4.95±1.46) pionts, (25.56±4.03) pionts; the information acquisition ability, interaction ability, willingness to improve health, willingness of financial support and total HeLMS scores were (36.77±4.85) pionts, (35.74±4.58) pionts, (15.07±1.55) pionts, (6.53±1.76) pionts, (94.12±6.44) pionts, significantly higher than in the control group [(34.37±5.84) pionts, (33.19±5.90) pionts, (14.23±1.43) pionts, (5.81±1.29) pionts, (87.60±9.18) pionts], the difference was statistically significant ( t values were 2.070-5.321, P<0.05). Conclusion:Shared decision making can effectively alleviate decisional conflict and improve health literacy of patients with stable coronary artery disease.