Objective To investigate the expression of transcription factor Oct 4 in gastric carcinoma and its effects on gastric cancer cell proliferation and apoptosis after Oct 4 gene interfered by lentivirus vector.Methods Real time PCR and Western blot were used to observe the expression of Oct 4 in different differentiated gastric cancer cell lines.Gastric cancer cell lines with high expression of Oct 4 was cultured and infected by siRNA-Oct 4-lentivirus vector.Cell proliferation and apoptosis were observed after Oct 4 gene was interfered.Results Oct 4 was highly expressed in poorly and moderately differentiated gastric cancer cells.Gene interfered with siRNA inhibits the expression of Oct 4 in gastric cancer cells and show significant effects on cell proliferation and mobility as well as apoptosis after down-regulation of Oct 4.Condusions Oct 4 expression is in close relationship with gastric cancer cell proliferation and invasive ability.

Objective To explore the effects of nursing intervention based on quantitative evaluation strategy on gastrointestinal function and quality of life in patients with gastric cancer after surgery. Methods Totally 94 gastric cancer patients who received surgery during January 2013 to December 2014 were enrolled in the study by using convenience sampling method. According to the random number table,the participants were divided into two groups. Patients in the control group (n=47) received routine perioperative nursing care,while patients in the intervention group (n=47) received quantitative evaluation based intervention care. The recovery of gastrointestinal function,complications,and the quality of life (QOL) (evaluated by QLQ-C30 scale) that three months after intervention were compared between two groups.Results In the intervention group,the time spent in abdominal distension improvement,bowel sound recovery,exhaust,and extubation was [(3.45±0.45) d,(2.86±0.49) d,(32.64±6.02) d and (3.59±0.26) d],and they were all shorter than that in the control group [(4.16±0.67) d,(3.57±0.53) d,(45.71±7.53) d and (5.17±0.84) d] (t=2.292, 2.412,2.559,2.084;P<0.05]. The intervention group had lower incidence of postoperative pulmonary infection compared with the control group (6.38% vs. 23.04%;χ2=5.371;P<0.05). After intervention,the QLQ-C30 scale indicated that the intervention group had higher scores in each dimension and higher total score than the control group (t=2.537,2.147,2.549,2.573,2.823,2.559;P<0.05).Conclusions The nursing intervention based on quantitative evaluation strategy can effectively improve the gastrointestinal function recovery and QOL of patients,and it can also reduce the related complications of patients with gastric cancer after surgery.

Objective:To evaluate the safety and feasibility of robot assisted total gastrectomy plus hand-sewn esophagojejunostomy.Methods:The clinical data of 72 patients diagnosed with gastric cancer and undergoing total gastrectomy at the First Affiliated Hospital of Ningbo University from Nov 2021 to May 2024 were retrospectively analyzed. They were divided into two groups: robot-assisted total gastrectomy (RATG) group, consisting of 30 patients, and laparoscopic assisted total gastrectomy (LATG) group, consisting of 42 patients . In the RATG group, the digestive tract was reconstructed by manual suture under the robot scope and Roux-Y reconstruction was performed . In LATG group, digestive tract reconstruction was performed using an in vitro stapler and Roux-Y. The clinicopathological data, perioperative indexes, and postoperative follow-up data of both groups were observed and analyzed.Results:All 72 patients successfully completed the operation without conversion to open laparotomy. The total operation time in RATG group was longer than that in LATG group [(235.2±25.8) min vs. (200.7±40.6) min, t=4.099, P<0.05)].RATG was superior to LATG group in terms of digestive tract reconstruction time, postoperative fluid intake time and hospitalization days,the difference was statistically significant [(36.9±3.0) min vs.(39.4±4.5) min, (4.2±0.5) d vs. (5.2±0.6) d、(9.5±1.6) d vs. (10.8±2.4)d, t=-2.554,-7.135,-2.595, all P<0.05]; In terms of postoperative pathology, the number of lymph node dissection in RATG group was higher than that in LATG group [(29.8±6.2) vs. (26.3±7.5), t=2.197, P<0.05]. Conclusion:The application of delayed disconnection hand-sewn esophagojejunostomy in Da Vinci robot total gastrectomy is safe and feasible for cure-intent total gastrectomy in patients of gastric carcinoma.

Objective To evaluate circulating tumor cells (CTC) in prognosis prediction of gastric cancer.Method Peripheral blood samples were obtained from 65 patients with gastric cancer and 20 normal volunteers.CTC were detected by using CellSearch(R) CTC Detecting System.Postoperative progress-free survival (PFIS) and overall survival (OS) were evaluated against CTC status and clinicopathological factors.Result CTC were positive in 35 out of 65 (53％) patients compared to 1 out of 20 (5％) healthy controls,P ＜ 0.05.CTC were not statistically corelated with patients' age,sex and lymph node status or distal metastasis,vascular invasion and tumor markers (all P ＞ 0.05).CTC positive patients had poorer OS (P ＜ 0.05) and PFS (P ＜ 0.05) compared with those with negative CTC.Conclusion Positive CTC in gastric cancer patients predict poor prognosis after radical gastrectomy.