1.Application of in vitro bionic digestion and biomembrane extraction for metal speciation analysis, bioavailability and risk assessment in lianhua qingwen capsule.
Lu-Xiu LIN ; Shun-Xing LI ; Feng-Ying ZHENG
China Journal of Chinese Materia Medica 2014;39(12):2330-2335
One of the causes of the high cost of pharmaceuticals and the major obstacles to rapidly assessing the bioavailability and risk of a chemical is the lack of experimental model systems. A new pre-treatment technology, in vitro bionic digestion was designed for metal analysis in Lianhua Qingwen capsule. The capsule was digested on 37 degrees C under the acidity of the stomach or intestine, and with the inorganic and organic compounds (including digestive enzymes) found in the stomach or intestine, and then the chyme was obtained. Being similar to the biomembrane between the gastrointestinal tract and blood vessels, monolayer liposome was used as biomembrane model Affinity-monolayer liposome metals (AMLMs) and water-soluble metals were used for metal speciation analysis in the capsule. Based on the concentration of AMLMs, the main absorption site of trace metals was proposed. The metal total contents or the concentration of AMLMs in the capsule were compared to the nutritional requirements, daily permissible dose and heavy metal total contents from the "import and export of medicinal plants and preparation of green industry state standards". The metal concentrations in the capsule were within the safety baseline levels for human consumption. After in vitro bionic digestion, most of trace metals were absorbed mainly in intestine. The concentration of As, Cd, Pb was 0.38, 0.07, 1.60 mg x kg(-1), respectively, far less than the permissible dose from the "import and export of medicinal plants and preparation of green industry state standards".
Biological Availability
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Capsules
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adverse effects
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pharmacokinetics
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Digestion
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Drugs, Chinese Herbal
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adverse effects
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pharmacokinetics
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Humans
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Metals, Heavy
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adverse effects
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pharmacokinetics
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Models, Biological
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Stomach
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metabolism
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Trace Elements
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adverse effects
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pharmacokinetics
3.The breeding and culture condition optimization of a high-biomass, selenium-enriched yeast strain.
Xiu-Ying FAN ; Xue-Na GUO ; Xiu-Hui FU ; Xiu-Ping HE ; Chang-Lu WANG ; Bo-Run ZHANG
Chinese Journal of Biotechnology 2003;19(6):720-724
The yeast fusant ZFF-28, which is high in biomass production and rich in selenium, was constructed after mutagenesis and protoplasts fusion between yeast strains. The total selenium content of ZFF-28 is 1.8 and 1.0 times higher than that of the parental strains Saccharomyces cerevisiae ZY-67 and Saccharomyces kluyveri SZY-198 respectively. Using single factor tests and a L16(4(3) x 2(1)) orthogonal design, the cultivation conditions was optimized as: 50mL culture in 250mL shake flasks in molasses containing 6% sugar and 60microg/mL Se at 28 degree C for 25h at 220 r/min, with the initial pH adjusted to 6.0 - 6.5. Under the optimized conditions, the biomass (dry weight) reached 8.2g/L and the Se content of the cells reached 2050microg/g, with organic and inorganic Se contents being 91% and 9% respectively.
Biomass
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Hydrogen-Ion Concentration
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Saccharomyces
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genetics
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growth & development
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metabolism
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Saccharomyces cerevisiae
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genetics
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metabolism
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Selenium
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metabolism
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Selenium Compounds
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metabolism
4.Determination of gamma-aminobutyric acid in aerial part of Panax notoginseng by HPLC.
Jing-Jing YANG ; Ying LIU ; Xiu-Ming CUI ; Yuan QU ; Lu-Qi HUANG
China Journal of Chinese Materia Medica 2014;39(4):606-609
The content of gamma-aminobutyric acid in the aerial part of Panax notoginseng in different productive area was determined by using high performance liquid chromatography. HPLC analysis was made on a C18 (4.6 mm x 250 mm,5 microm) with acetonitrile and water containing 4.1 g x L(-1) sodium acetate as mobile phase. The detection wavelength was 254 nm. The HPLC method showed good linearity within the range of 0.01 - 1.03 g x L(-1). The average recovery of GABA in the stems and leaves of P. notoginseng and the flowers of P. notoginseng was 101.7% (RSD 1.1%, n = 3) and 97.3% (RSD 0.38%, n = 3), respectively. The contents of GABA in the samples of different productive areas were not significant different, and the average contents of GABA in the stems and leaves of P. notoginseng and the flowers of P. notoginseng were 0.49% and 0.53%. This method was simple and reliable, and it was suitable for the determination of gamma-aminobutyric acid in the aerial part of P. notoginseng.
Chromatography, High Pressure Liquid
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methods
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Flowers
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chemistry
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Panax notoginseng
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chemistry
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Plant Extracts
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analysis
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Plant Leaves
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chemistry
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gamma-Aminobutyric Acid
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analysis
5.Analysis and evaluation of nutritive elements in aerial part of Panax notoginseng.
Yuan QU ; Ying LIU ; Lu-Qi HUANG ; Lan-Ping GUO ; Xiu-Ming CUI
China Journal of Chinese Materia Medica 2014;39(4):601-605
OBJECTIVETo make full use of the plant resources of Panax notoginseng, nutritional compositions and mineral elements were analyzed in aerial part of P. notoginseng from different areas in Yunnan.
METHODUsing the national standard method, water, ash, crude fat, crude fiber, crude protein and mineral elements were determined in aerial part of P. notoginseng from different growing areas.
RESULTResults showed that there were higher contents of crude fiber and crude protein, and lower content of crude fat in the stems and flowers of P. notoginseng. Meanwhile, a large number of mineral elements were determined in two locations of P. notoginseng, and the contents of Zn, Fe, Mn, Ca and Mg were obvious higher among these mineral elements.
CONCLUSIONThis study showed that the stems and flowers of P. notoginseng were nutritious and suggested that the aerial part may be utilized as new resources foods.
Flowers ; chemistry ; Nutritive Value ; Panax notoginseng ; chemistry ; Plant Extracts ; analysis ; Plant Leaves ; chemistry ; Plant Stems ; chemistry ; Trace Elements ; analysis
6.The application of GlideScope videolaryngoscope in head neck tumor operations with difficult tracheal intubation.
Xiu-ying ZHANG ; Guang-hua ZHANG ; Lu-bo GAO
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2006;41(12):950-951
Adult
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Aged
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Aged, 80 and over
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Female
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Head and Neck Neoplasms
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surgery
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Humans
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Intubation, Intratracheal
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methods
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Laryngoscopes
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Male
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Middle Aged
7.Investigation on the origin of 5-HMF in Shengmaiyin decoction by RP-HPLC method.
Journal of Zhejiang University. Science. B 2005;6(10):1015-1021
The origin of 5-HMF (5-hydroxymethyl-2-furaldehyde) in a Shengmaiyin decoction was investigated by the RP-HPLC method below. A C(18) column (250 mm x 4.6 mm, i.d. 5 microm) with a column temperature of 25 degrees C was used. The mobile phase was a mixture of ultra-pure water-acetonitrile (95:5, V/V) and the flow rate was 1.0 ml/min. The detection wavelength was 280 nm. The injection volume was 1 microl and the running time was about 20 min. The addition of Schisandra was regulated to assess the contribution of an acid environment to the production of 5-HMF. In order to confirm the role of saccharides in the production of 5-HMF, different amount of fructose was used. The 5-HMF level in decoctions of processed and unprocessed Schisandra was investigated in order to determine the origin of 5-HMF. The results showed that 5-HMF was derived mainly from the decoction of Schisandra only and not the mixed decoction of Ophionpogon and Schisandra. The appearance of 5-HMF is not simply the result of the decomposition of saccharides under the acid environment created by Schisandra, but the processing procedure plays an important role in the production of 5-HMF.
Chromatography, High Pressure Liquid
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Drug Combinations
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Drugs, Chinese Herbal
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chemistry
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Fructose
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metabolism
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Furaldehyde
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analogs & derivatives
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chemistry
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isolation & purification
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Hydrogen-Ion Concentration
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Schisandra
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chemistry
8.Subdiaphragmatic vagotomy reduces the responses of fever and discharge of neurons in PVN to LPS.
Xiu-ying LU ; Gui-zhen YANG ; Wen JIANG
Chinese Journal of Applied Physiology 2002;18(4):337-339
AIMTo study the possibility that responses of fever and discharge of neurons in PVN to intraperitoneal administration of LPS are mediated by vagal afferents.
METHODSRectal temperature of rat was detected by digital temperature detecting instrument. Glass micropipette placed in PVN was used to record unit discharges of neurons in it, before and after LPS was injected into PVN in normal rats and vagotomy rats.
RESULTSThe rectal temperature change value in vagotomy LPS group was significantly decreased compared with that in sham LPS group, and there was striking difference between them (P < 0.05). The discharges of neurons in PVN was increased in the normal rat in response to LPS. The discharges of neurons in PVN had no significant change in the vagotomy rats in response to LPS.
CONCLUSIONThe results indicate that vagus nerve may be one of the pathways of peripheral LPS signal communicating to CNS.
Animals ; Diaphragm ; innervation ; Fever ; chemically induced ; physiopathology ; Lipopolysaccharides ; Male ; Neurons ; physiology ; Paraventricular Hypothalamic Nucleus ; physiopathology ; Rats ; Rats, Wistar ; Vagotomy, Truncal
10.The efficacy of 125I radio active particle implantation in the treatment of maxillofacial malignancy of 43 cases
Xiu YAO ; Ling GU ; Ying LIU ; Shuangshuang LI ; Dong WANG ; Lu YANG ; Lihong XU ; Chongjian FU
Journal of Practical Stomatology 2016;32(2):220-224
Objective:To investigate the effects of 125I radioactive particle implantation in the treatment of maxillofacial malignancy. Methods:43 patients with maxillofacial malignancy were treated with 125I radioactive particle implantation.The procedure was carried out according to the treatment planning system(TPS),with the particles spaced uniformly at 1 cm intervals and with the activity of 0.7 m Ciby 41 particles per case on a verage.All patients were followed up for 6-60 months.Results:The whole treatment procedurewas successful,and no particle displaced.The follow-up rate was 93.02% and treatment effective rate(CR+PR) was 90.70%.Norecur-rence was foundinall target areas.The mortality due to tumor was 9.30%and total survival rate was 74.43%.The cumulative survival rate of the patients in 0.5,1,2,3 and 5 years was 93.0%,85.7%,79.3,69.8% and 56.9% respectively.Survival periodon aver-age was 36.06-50.04 months,with the median of 43.05 months.The longest tumor-free survival period was 60 months.Radiation in-jury rate was 20.93% and only level 1 radiation injury was observedinall the cases.Facial nerve dys function was found in 2 cases and recovered after treatment.Conclusion:Treatment of maxillofacial malignancy by implantation of 125I particles is convenient and mini-mally invasive.The treatment canincrease survival rate of the patients and guaran tee the oropharynxes' function.