The papers regarding moxibustion published in science citation index (SCI) journals for the recent 5 years were searched to explore the international tendency of moxibustion researches, which provided references for moxibustion to have a better internationalization. With methods of internet search and database search, a total of 116 papers regarding moxibustion were included. These papers were published in 40 kinds of journals, mostly in Britain and the United States. The journal with the highest impact factor was Stroke, which had 5729 points. The number and impact factor of these journals were inferior to those of acupuncture journal. Compared among these journals, the depth and width of moxibustion research were increasing year by year. The category of diseases related with moxibustion is mainly digestive system diseases, motor system diseases and urinary-genital system diseases. The type of papers was characterized with clinical observation and mechanism research. Researches related with moxibustion included moxibustion dose and safety. It was believed that the international recognition of moxibustion effectiveness, standardization of moxibustion manipulation, standardization research, etc. were needed to be solved in the further.
Bibliometrics ; Clinical Trials as Topic ; Humans ; Journal Impact Factor ; Moxibustion ; Publications ; statistics & numerical data

Objective To optimize the formulation ratio and preparation process of the APGA modified artemether liposomes, and evaluate its physical and chemical properties. Methods The encapsulation efficiency of artemether was evaluated as index, and the preparation method of APGA modified artemether liposomes was optimized. The preparation process of APGA modified artemether liposomes was optimized by orthogonal experiments. Laser particle analyzer and transmission electron microscopy were used to evaluate the particle size, Zeta potential, and appearance of liposomes, and dialysis method was used to study the release of liposomes in vitro. Results The best prescription was as follow: EPC-Chol-TPGS at 95:0.5:3, 5% APGA-PEG-DSPE, the artemether-lipid ratio at 1:20, film-forming temperature 30 ℃, probe ultrasound time 8 min. The resulting liposomes exhibited a pale blue opalescent appearance. The average particle size, polydispersity index, and zeta potential of artemether liposomes was (99.97 ± 1.67) nm, 0.185 ± 0.021, and (-0.023 ± 0.080) mV, respectively. Transmission electron micrograph image showed that artemether liposomes were spherical vesicles with uniform sizes. The encapsulation efficiency of artemether in liposomes was (90.06 ± 1.15)%. In vitro cumulative release rate of artemether from the liposomes in the simulated body fluids was (57.07 ± 6.09)% after 48 h. Conclusion The optimized APGA modified artemether liposomes was successfully developed. It had the following characters: round shape, uniform particle size, high drug encapsulation efficiency and good sustained-release effect.

Objective To evaluate the targeting and inhibitory effects of the targeted artemether liposomes modified with APGA- PEG2000-DSPE conjugate in vitro. Methods APGA was used as targeted molecular, and the new functional material APGA- PEG2000-DSPE conjugate was synthesized and confirmed by using MALDI-TOF-MS. A kind of targeted artemether liposomes was developed by modifying APGA-PEG2000-DSPE using film dispersed method. Co-culture model of BBB-glioma C6 tumor spheroids was developed, and it was used to study the transporting efficiency across the BBB and the penetration ability of the liposomes into glioma C6 tumor spheroids viewed by a confocal laser scanning microscope. Flow cytometry was used to evaluate the targeting properties of the preparations to C6 glioma cells. SRB method was used to study the inhibitory effect of targeted liposomes on the growth of C6 glioma cells. Results The APGA-PEG2000-DSPE conjugate was confirmed by using MALDI-TOF-MS, and its average mass was 3 150. The co-culture model showed that the targeted liposomes can better transport across the BBB and then penetrate into the glioma C6 tumor spheroids. To C6 glioma cells, the IC50 of the targeted artemether liposomes, artemether liposomes and free artemether were 45, 82, and 95 μmol/L, separately. Conclusion The targeted artemether liposomes could effectively transport across the BBB and penetrate into the glioma C6 tumor spheroids. The targeted artemether liposomes had stronger inhibitory effect on brain glioma cells in vitro.

Aged ; Coal Mining ; Electrocardiography ; Heart Rate ; physiology ; Humans ; Male ; Middle Aged ; Pneumoconiosis ; physiopathology

Aged ; Arrhythmias, Cardiac ; diagnosis ; physiopathology ; Coal Mining ; Electrocardiography ; Humans ; Male ; Middle Aged ; Pneumoconiosis ; complications ; physiopathology

Objective:To compare psychosocial factors and immune function between patients with breast cancer and benign tumor before operation.Methods:The patients of breast tumor(56 case)were measured by clinical psychological rating scale(EPQ、HAMD、HAMA、LES、SSRS、DSQ)before operation,and their serum levels of IgA, CD_4~+,CD_(19)~+ and the activity of NK cell were measured.The patients of breast tumor were divided into breast cancer group(36 case)and benign tumor group(22 case)bases on results of pathology.Results:In breast cancer group, there were significantly higher scores of neurotic,total and negative life events,immature defense styles,HAMA and HAMD than those in benign tumor group;while NK cell activity,levels of CD_4~+ and CD_(19)~+ and the score of mature defense,social supports,subjective supports and utility degree of supports were marked lower.There were significantly correlation between immune function(NK cell activity,content of CD_4~+,CD_(19)~+,IgA)and psychosocial factors(social support,P score of EPQ,HAMD and HAMA total score,quantity of negative life events etc). Conclusion:Cancer patients had more server psychosocial problems and lower immune function.The immune function of patient with breast cancer before operation is associated with the various psychosocial factors.

Adult ; Humans ; Male ; Sleep Apnea Syndromes

One of the causes of the high cost of pharmaceuticals and the major obstacles to rapidly assessing the bioavailability and risk of a chemical is the lack of experimental model systems. A new pre-treatment technology, in vitro bionic digestion was designed for metal analysis in Lianhua Qingwen capsule. The capsule was digested on 37 degrees C under the acidity of the stomach or intestine, and with the inorganic and organic compounds (including digestive enzymes) found in the stomach or intestine, and then the chyme was obtained. Being similar to the biomembrane between the gastrointestinal tract and blood vessels, monolayer liposome was used as biomembrane model Affinity-monolayer liposome metals (AMLMs) and water-soluble metals were used for metal speciation analysis in the capsule. Based on the concentration of AMLMs, the main absorption site of trace metals was proposed. The metal total contents or the concentration of AMLMs in the capsule were compared to the nutritional requirements, daily permissible dose and heavy metal total contents from the "import and export of medicinal plants and preparation of green industry state standards". The metal concentrations in the capsule were within the safety baseline levels for human consumption. After in vitro bionic digestion, most of trace metals were absorbed mainly in intestine. The concentration of As, Cd, Pb was 0.38, 0.07, 1.60 mg x kg(-1), respectively, far less than the permissible dose from the "import and export of medicinal plants and preparation of green industry state standards".
Biological Availability ; Capsules ; adverse effects ; pharmacokinetics ; Digestion ; Drugs, Chinese Herbal ; adverse effects ; pharmacokinetics ; Humans ; Metals, Heavy ; adverse effects ; pharmacokinetics ; Models, Biological ; Stomach ; metabolism ; Trace Elements ; adverse effects ; pharmacokinetics

Aged ; Aged, 80 and over ; Cross Infection ; etiology ; Diabetes Mellitus, Type 2 ; complications ; microbiology ; Humans ; Male ; Middle Aged ; Pneumoconiosis ; complications ; microbiology ; Retrospective Studies

0.05),and NK cells function(CD56),humoral immunologic function(IgG,IgM,IgA)were significantly elevated after TP-5 administration for 3 months(Pa