Lack of domestic medical humanistic education in medical schools leads to the low humanistic quali-ty of medical students, but humanistic quality of medical personnel is essential to medical activities. Taking into ac-count the actual domestic medical schools, for medical students, humanistic quality education in social practice should be the only way, which encompasses guiding medical students to pay attention to people′s livelihood, build-ing a social practice brand combined medicine and humanities, and implementing dynamic observation of humanis-tic education.

This paper stated the main contents of hospital cultural integration beginning of the necessary of it. Hospital cultural integration contained concept of symbiosis, management upgrade, strategic reorganization, recon-struction of responsibilities and rights, code of conduct, etiquette unity. The authors pointed out the current prob-lems of cultural integration:ideological understanding was lacking combined with the preference to technology over humanities;the integration mechanism was not perfect and the adaptation was terrible;integration planning was not systematic combined with the lack of investigation and research. The paper also put forward concrete paths to a-chieve the cultural integration in the process of construction of regional medical association:develop deep practical cultural integration programs;make policy to stabilize human resources;strengthen exchange of middle-level ca-dre posts;create an atmosphere that all persons participate in cultural integration.

Codon ; Computational Biology ; methods ; Evolution, Molecular ; Influenza A virus ; classification ; genetics ; Influenza Vaccines ; immunology ; Molecular Biology ; Phylogeny ; RNA, Viral ; chemistry ; Viral Proteins ; chemistry

To observe the effect of autologous stem cell transplantation in diabetic retinopathy.
●METHODS:Totally 58 cases (116 eyes) who underwent autologous stem cell transplantation were confirmed as no diabetic retinopathy (18 eyes), mild non-proliferative diabetic retinopathy (NPDR) (41 eyes), mid-level NPDR (51 eyes); severe NPDR (6 eyes) by ophthalmoscope directly or indirectly and fluorescence fundus angiography (FFA ). Follow - up was 6 - 12mo, the changes of retinopathy were observed.
●RESULTS: The total effective rate of vision and retinopathy was 84. 4%, 76. 7%. The results of severe NPDR was statistically worse than the other groups ( P <0. 05).
●CONCLUSlON: The stable blood glucose level and improved pancreatic function after autologous stem cell transplantation might be helpful in diabetic retinopathy, the long effects need to be researched further.

The viable but noncuturable (VBNC) state is a survival strategy when bacteria are exposed to en- vironment stress. The VBNC state is part of the life cycle of non-differentiating bacteria, and it has a far-reaching impact on traditional bacteriology. Cells in the VBNC state fail to grow on the routine bacterio- logical media, here its significance in human health and environment science are detailed. Cells entering the VBNC state exhibit dwarfing and a number of metabolic changes in respiration rates and macromolecular synthesis. This paper summarized the variations in DNA and protein comparing to the culturable cells. We also discussed the ability of cells to resuscitate from the VBNC state and return to an actively metabolizing and culturable form. Some new methods for monitoring the VBNC state were listed. Finally the future was suggested.

Objective:To study the in vivo anti-tumor effect of exosomes (Exo) combined with bacillus Calmette-Gu?rin vaccine(BCG). Methods:Exo was isolated and purified from culture supernatant of E.G7-OVA tumor cells by density gradient centrifugation. Protein components of Exo were detected by Western blotting. Exo,BCG,Exo combined with BCG (Exo+BCG) or PBS were pre-injected into mice before injection of E.G7-OVA cells,and the anti-tumor effects were observed in each group. Mouse model bearing E.G7-OVA cells was established to examine the immuno-therapy effects of Exo with or without BCG. Cytotoxity of spleen CTL was measured by LDH in different groups. Results:Exo derived from E.G7-OVA cells contained HSP60,OVA,HSC70 and CD63 as detected by Western blotting. Tumor-free rate at 90 d was significantly higher in Exo+BCG vaccinated mice than those in Exo or BCG vaccinated mice as measured by immuno-protective assay (60% vs 20% or 0%,P

Acute Disease ; Coronary Aneurysm ; diagnostic imaging ; etiology ; Female ; Humans ; Infant ; Intracranial Hemorrhages ; diagnosis ; diagnostic imaging ; etiology ; Male ; Mucocutaneous Lymph Node Syndrome ; complications ; diagnosis ; diagnostic imaging ; Tomography, X-Ray Computed

Objective To observe the effect of Yishen Paizhuo Decoction(Decoction for reinforcing the kidney and discharging the turbid)on chronic renal failure(CRF)patients.Methods The 82 CRF patients were randomized into observation group(42 cases) and control group(40 cases).In addition to the routine treatment,the observation group was administered modified Yishen Paizbuo Decoction,and the control group was given coated aldehyde oxystarch.The course of treatment was 6 months for both groups. Changes of clinical symptoms,renal functions,blood fat,and hemoglobin were observed,and the linear regression evaluation and renal survival evaluation were employed to evaluate the development of CRF.Results The improvement of observation group in renal func- tions and blood fat was remarkable with the increased renal survival rate,compared with the control group,the difference was signifi- cant(P

OBJECTIVE: To investigate the effect of inotodiol in inducing apoptosis of human ovary cancer cell line SKOV3, and its influence on the related apoptosis proteins such as Bcl-2, Bax and caspase-3. METHODS: Human ovary cancer SKOV3 cells were cultured in vitro. Cytotoxcity of inotodiol was determined by MTT assay. Morphological changes of apoptotic cells were observed by transmission electron microscope(TEM). Apoptotic rates of SKOV3 cells were confirmed by TUNEL(terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling) assay. The expressions of Bcl-2, Bax and caspase-3 were detected by immunocytochemical staining method. RESULTS: Inotodiol can inhibit the SKOV3 cells growth in a time-and dose-dependent manner. After treatment with inotodiol, SKOV3 cells showed typical apoptotic features under TEM. After human ovary cancer cell line SKOV3 were treated with inotodiol in concentration of 50 mg·L-1 for 24 and 48 h, their apoptotic rates were(21.91 ± 2.84)% and(43.83 ± 3.22)%, respectively. The apoptotic rate of SKOV3 cells induced by inotodiol increased in a time-dependent manner(P < 0.05). Immunocytochemical staining showed that the expression of Bcl-2 decreased obviously, whereas those of Bax and caspase-3 increased significantly in inotodiol group, compared with the control group. CONCLUSION: Inotodiol can inhibit the proliferation and induce the apoptosis of human ovary cancer cell line SKOV3 through up-regulation of Bax protein, down-regulation of Bcl-2 protein and activation of caspase apoptosis signal.

Objective To investigate the effect of exercise load on apparent diffusion coefficient (ADC) and fractional anisotropy (FA) of normal lumbar intervertebral discs in magnetic resonance (MR) diffusion tensor imaging (DTI) . Methods Thirty healthy volunteers (24 males and 6 females, aged 19 to 25 years) underwent examinations with MR T2WI and DTI of the lumbar intervertebral discs before and after exercise load. Pfirrmann grading was evaluated with T2WI, and the B0 map, ADC map and FA map were reconstructed based on the DTI data to investigate the changes in ADC and FA after exercise. Results Of the 30 volunteers (150 intervertebral discs) receiving the examination, 27 with discs of Pfirrminn grade II were included for analysis. In these 27 volunteers, the average ADC and FA before exercise were (1.99 ± 0.18) × 10- 3 mm2/s and 0.155 ± 0.059, respectively. After exercise, ADC was lowered significantly to (1.93 ± 0.17) × 10-3 mm2/s (P<0.05) and FA increased slightly to 0.1623 ± 0.017 (P>0.05). Conclusion DTI allows quantitatively analysis of the changes in water molecular diffusion and anisotropy of the lumbar intervertebral discs after exercise load, which can cause a decreased ADC and a increased FA value, and the change of ADC is more sensitive to exercise load.