Peroxisome proliferation-activated receptor-? (PPAR-?) is a ligand-activated transcription factor belonging to the nuclear hormone receptor superfamily and participates in the regulation of various metabolic pathways as well as inflammatory responses. PPAR-? ligands significantly improve myocardial functional recovery and prevent ischemia-reperfusion induced injury. Given the increasing understanding of the cardioprotective effects of PPAR-? ligands,we know today that the therapeutic effects of PPAR-? ligands reach far beyond their use as insulin-sensitizers,as many of these agents exert beneficial effects in the conditions associated with ischemia-reperfusion and inflammation.

Peroxisome proliferation-activated receptor-γ (PPAR-γ) is a ligand-activated transcription factor belonging to the nuclear hormone receptor superfamily and participates in the regulation of various metabolic pathways as well as inflammatory responses. PPAR-γ ligands significantly improve myocardial functional recovery and prevent ischemia-reperfusion induced injury. Given the increasing understanding of the cardioprotective effects of PPAR-γ ligands, we know today that the therapeutic effects of PPAR-γ ligands reach far beyond their use as insulin-sensitizers, as many of these agents exert beneficial effects in the conditions associated with ischemia-reperfusion and inflammation.

AIM: To extract Zanthoxylum oil from the seeds of Zanthoxylum bungeanum Maxin, and find out a better extraction method and its optimized operating conditions. METHODS: Zanthoxylum oil was extracted by distillation method, solvent extraction and supercritical fluid extraction, respectively. The products gained in each experiment was analyzed by GC after it had been methyl esterified. RESULTS: The yield of Zanthoxylum oil extracted by distillation, solvent extraction and supercritical fluid extraction was 0.88 %, 13.73 % and 13.52 %, respectively, and its content of unsaturated fatty acid 4.50 %, 65.97 %, 74.97 %, respectively. CONCLUSION: Supercritical fluid extraction was the better of the three mehtods, whose optimized operating conditions consisted of 30 MPa pressure, 50 ?C operating temperature and 5 h extraction time.

11.0 mmol/L).Perioperative decontamination in digestive tract was a protective factor in the prevention of bacterial infection.Conclusion Bacterial infection is one of the most severe complications after OLT.Therefore,it is very important to remove those risk factors,make early diag- nosis and take effective treatment.

Objective To investigate the significance of thyroid function screening in high-risk pregnant women with gestational diabetes (GDM) in early pregnancy.Methods A total of 194 cases with GDM were selected as our subjects.The patients were divided into group A(three normal items,a total of 109 cases),group B (one abnormal item,a total of 57 cases) and group C (two abnormal items,a total of 28 cases).The levels of serum anti-thyroglobulin antibody (TGAb),anti-thyroid peroxidase antibody (TPOAb),serum three triiodothyronine(TT3),thyroxine (TT4),free three triiodothyronine (FT3),free thyroxine (FT4) and thyroidstimulating hormone (TSH) were screened.Results TSH levels in group A was (1.45 ± 0.43) mU/L,significantly lower than in group B and group C((1.77±0.53),(1.89±0.74) mU/L).FT4 levels in group A was (11.62±0.98) nmol/L,significantly higher in group B and group C((10.23±0.75),(9.87±0.88) nmol/L)).Proportion of TPOAb,TGAb positive in group A were 9.17％(10/109) and 21.05％(12/57),significantly lower than that of group B and group C((28.57％(8/28) and 3.67％(4/109),7.02％(5/57) and 17.86％(5/28)).And the differences were significant (P＜ 0.05).And TPOAb + TGAb in group A was 0.92％(1/109),significantly lower than that of group B and group C(7.02％ (4/57),17.86％ (5/28);P ＜0.05).Conclusion The importance of screening thyroid function in early pregnancy in women at high risk for gestational diabetes is worthy of clinical promotion.

Objective To investigate the functions of Monocyte chemotactic protein-induced protein 1 (MCPIP1) in human breast cancer cell line MDA-MB-231.Methods MDA-MB-231 cells were transfected with GFP-tagged MCPIP1 by Tet-on inducing expression system.Endogenous MCPIP1 was knocked down by stable expressing shRNA.MTT assay was performed to measure the growth of MDA-MB-231 cells after overexpression or knockdown of MCPIP1.FACS method was used to analyze cell cycle in MDA-MB-231 cells.Real-time PCR was used to test the expression of cell cycle-related mRNAs expression and their half-lives.RNA-IP experiment was conducted to detect the mRNA directly enriched by MCPIP1.Luciferase assay was performed to determine whether the mRNA decay was mediated through 3′UTR.Results MCPIP1 overexpression significantly inhibited cell proliferation(P<0.05), while knockdown MCPIP1 promoted cell proliferation with statistical significances (P<0.05).MCPIP1 induced cell cycle arrest in MDA-MB-231 with statistical significance (P<0.01).MCPIP1 overexpression reduced the half-lives of cell cycle mRNAs (CDK2,CDK6,cyclin D1,cyclin E1,respectively) with significance (P<0.01).In addition, cell cycle-related mRNAs were able to be pulled down by GFP-MCPIP1 but not isotype IgG(P<0.05).Compared with control vector, MCPIP1 significant suppressed luciferase activities of all four 3′UTR reporters (P<0.05).Conclusions MCPIP1 functions as a tumor suppressor in human breast cancer cell line MDA-MB-231 through inducing G1 cell cycle arrest.

Animals ; Cytoprotection ; Humans ; Ischemic Preconditioning ; Reperfusion Injury ; prevention & control ; therapy

Acupressure ; Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Aged, 80 and over ; Combined Modality Therapy ; Female ; Hiccup ; etiology ; therapy ; Humans ; Male ; Middle Aged ; Neoplasms ; complications

Humans ; Myocardial Reperfusion Injury ; prevention & control ; Translational Medical Research

Cerebral Cortex ; physiology ; Electroencephalography ; Humans ; Magnetic Resonance Imaging ; Positron-Emission Tomography ; Taste Perception ; physiology