Objective　To summarize the experiences in caudate lobectomy in five cases of hepatic carcinoma.Methods　Five cases of primary liver caudate lobe cancer resected in the last five years were reviewed.Some problems including anatomy,the biological characteristics of the caudate lobe cancer,surgical approach,blocking of the liver blood supply were discussed.Results　One case died on the 11th postoperative day,the other four cases have been followed up for five years,two years and nine months,two years,one year and six months,respectively.They are all survived.Conclusion　Liver cancer located at the caudate lobe can be successfully resected as those located in other regions with a clear anatomy and a full exposure.

24 h) was the main cause of high emergent perioperative death rate. There was a rationale for prophylactic surgery.

Photo images,as a useful visual aid,are frequently seen in medical journals.This paper discusses the importance of photo in medical papers.Problems with the photos are discussed,which includes absence of caption,the illegibility of the photos,the inferior contrasts,absence of the unusual changes,etc..Suggestions are given to improve photo effect so as to improve the quality of medical papers.

Evidence based medicine is based on the high quality evidence from medical journals.In this paper,the progress of evidence based medicine(EBM)and its effects on clincal practice and research are introduced,and the necessary of carrying out editorial examination and appraisal on statistical problems in medical articles is discussed.

Objective To investigate the postoperative changes of serum proteins and immune responses in critical patients and the effect of recombinant human growth hormone(rhGH).Method 45 critical patients were divided randomly into rhGH group (15 cases) and control group (30 cases) after operation. Patients in rhGH group were given rhGH 8*!U/d,im for 7 days starting 48 hours post operation. Result The postoperative level of serum transferin,albumin and prealbumin decreased in all patients, and so did the activity of CD 3,CD 4 ,CD 8,NK and the ratio of CD 4/CD 8 .Serum prealbumin level increased significantly in rhGH group at day 4 and after ( P

Objective To observe the changes of plasma renin activity (PRA)in cirrhotic patients with ascites after portacaval shunt. Method Portal vein, artery,and peripheral vein PRA levels were measured in 16 cirrhotic patients with ascites during perioperative period of portacaval shunt. Results were compared with that in 16 cases of GI tract carcinoma.Results Z] (1)Measured at postshunt day 7,the portal venous pressure (PVP) was significantly lower than that preoperatively〔(26?4)?cm?H 2O vs. (36?4)?cm?H 2O, P

Objective To investigate the effect of Shenfu injection on ischemia reperfusion injury of rat liver graft. Methods Male SD rats were used as isogeneic orthotopic liver transplantation. Sixty rats were randomly divided into two groups(30 in each). The recipient received Shenfu injection intravenously immediately before the removal of the liver in SF group and normal saline of the same volume in control group. At 3、6 and 24h after the reperfusion blood and hepatic tissue were sampled for examination. Results The levels of superoxide dismutase (SOD) and nitric oxide (NO) in SF group significantly increased compared with those in control group(P

Objective To investigate the effects of interleukin 10(IL 10) on the intercellular adhesion molecule 1(ICAM 1) expression of rat hepatic stellate cells. Method Hepatic stellate cells were isolated and purified from rat liver by collagenase Ⅳ perfusion and density gradient centrifugation with Nycodenz. Passaged hepatic stellate cells were divided randomly into four groups: control group (A group), TNF? 100 U/ml group (B group), TNF? 100 U/ml plus IL 10 2 ng/ml group (C group). TNF? 100 U/ml plus IL 10 20 ng/ml group (D group). 24 h after incubation expression of ICAM 1 protein and mRNA in hepatic stellate cells were detected by using cell ELISA and semi quantitative RT PCR method. Results The yield rate of hepatic stellate cells was 2.5?10 7 per rat. The cell viability was above 98%. The purity of hepatic stellate cells was more then 92%. Expression of ICAM 1 protein (1.400 ?0.077) and mRNA(1.301?0.095) in B group were significantly higher than that in A group [0.559?0.071 ( P

ObjectiveTo use MAGE-1 antigen as tumor vaccine for the treatment of hepatocellular carcinoma (HCC).MethodsIrradiated peripheral blood mononuclear cells (PBMCs) pulsed with a MAGE-1 peptide (NYKCRFPEI) were used as antigen presenting cells (APC). The PBMCs from the same patient were stimulated with APCs on every 7th day for 4 times to elicit cytotoxic T lymphocytes (CTLs). The cytotoxicity of CTLs to various kinds of target cells was detected with the method of lactate dehydrogenase (LDH) releasing assay. Results The number of PBMCs increased by 32 folds during 28 days of culture. When effective cells: target cells (E∶T) was 10∶1, CTLs exhibited 62.5% cytotoxicity against autogenous lymphoblasts pulsed with the peptide of MAGE-1 antigen (NYKCRFPEI), 40.25% cytotoxicity against cells of BEL7405, a HCC cell line expressing both MAGE-1 and HLA-A24, compared with 17.88% cytolysis observed against autogenous lymphoblasts, 19.55% against cells of HLE, a HCC cell line expressing MAGE-1, negatively expressing HLA-A24. The cytotoxicity against cells of QGY7701, a HCC cell line expressing neither MAGE-1 nor HLA-A24 was much lower (1.6%). When E∶T was 3.3∶1, the cytotoxicity of CTL against peptide pulsed lymphoblasts was 53.6%, while against autogenous lymphoblasts, cells of HLE and cells of QGY7701 was much lower, 15.6%, 13% and 1% respectively. After 4h culture, most cells of BEL7405 were adhered with several CTL, few cells of QGY7701 were adhere with CTL. ConclusionsMAGE-1 peptide NYKCRFPEI in vitro successfully induced CTL with the ability of specifically killing target cells expressing both MAGE-1 and HLA-A24.

ObjectiveTo evaluate the effect of combined gene transfection with p53,p16 and p21 on the growth of human liver cancer cell lines.MethodsAfter transducing adeno associated virus(AAV) mediated p53, p16 or/and p21 genes to human hepatic carcinoma cell lines HLE,HepG2,QGY 7701,QGY 7703,BEL 7402,SMMC 7721, gene expression and tumor inhibition were studied in vitro and in BALB/c mouse model.ResultsAdeno associated virus mediated p53, p16 or p21 encoding gene could express in BEL 7402 cell line. Each individual type of recombinant AAV effected a significant induction of tumor cell apoptosis both in vitro and in vivo, the rate of apoptotic cells in vitro is about 30% and that of tumor growth inhibition is about 30～44%. And the apoptosis inducing efficiency was the highest after the tumor cell line was transfected by three recombinant AAV simultaneously, with a rate of 56% (in vitro) and 65% (in vivo).ConclusionNot only could all of the exogenous wild type p53, p16 and p21 genes mediated by AAV inhibit the growth of liver cancer cell lines, but also can the efficiency be significantly elevated by combined gene transfection.