[Objective] To study the microstructure of ligamentum fiavum of the early dynamic imbalanced rats.[Methods]The dynamic imbalanced rat mode was established.The samples were taken and studied by electron microscopy at 3、5、15、30、60 days after operation.[Results]A large number of fibroblasts in the ligamentum flavum were found at 3 days after the neck muscles were resected.The fibroblasts had an active metabolism,and around them the collagen fibers were markedly increased at 5 days.The number of fibroblasts was reduced,but the matrices were increased at 15 days.At 30 days,fibroblasts became slender,and fibers looked like waves.At 60 days,the number and appearance of the fibroblasts of the ligamentum fiavum were similar to that of the control group.The fibers were regularly arranged and became larger than that of the colltrol group.[Conclusion]The number of fibroblasts and the collagen fibers in the ligamentum flavum of the dynamic imbanlanced rats has a regular change after operation.

Objective To investigate that whether the immuno-modulatory capacity is intact in myelodysplastic syndrome-refractory anemia (MDS-RA) derived mesenchymal stem cell (MSCs) and what are the differences compare with those from normal donors.Methods Isolating MSC from MDS and normal donors respectively and exam their differences on T lymphocyte activation,proliferation and suppression.Results The results showed that the capacity of suppressing T cell proliferation and activation is weakened.Conclusion We propose that MSCs from pathological environment might be abnormal and should not be used for autologous transplantation.

ObjectiveTo evaluate mammaglobin (hMAM) mRNA expression as a tumor marker for micrometastatic carcinoma cells in the peripheral blood of patients with breast cancer (BC). MethodsTotal RNA extracted from a breast carcinoma cell line SKBR3 was subjected to analysis of hMAM mRNA expression by RT-PCR. The sensitivity of FQ-PCR to detect a SKBR3 cell at a level of 10 7 was established. Peripheral blood of 63 BC patients was examined by this method. ResultsBlood samples were positive in 19 out of 63 (30%) patients with breast carcinomas. None of the patients with other cancer (25 cases) or benign breast disease (13 cases) was positive and only 1 out of 31 healthy volunteers was found with detectable hMAM mRNA expression in the peripheral blood. ConclusionFluorescence quantitative (FQ) PCR combined with nest PCR was a sensitive method in detecting micrometastases of breast cancer.

ObjectiveTo evaluate portal vein chemotherapy for the treatment of hepatocellular carcinoma with portal vein tumor thrombi(PVTT).MethodsFourty five HCC patients with tumor thrombi in the main portal vein or the first branches undergoing surgical resection were divided into two groups receiving postoperative intraarterial and intraportal chemotherapy (21 cases), and intraarterial chemotherapy only (24 cases).ResultsThe survival rates of 6,12,18 months was 86%, 76% and 48% in the treatment group compared with 75%, 58% and 33% in the control group respectively. There were significant difference between the two groups.Conclusion Hepatectomy for HCC with removal of tumor thrombi and transcatheter hepatic arterial and portal vein chemotherapy is an effective treatment for HCC with PVTT.

Objective: To study the effects of morphine hydrochloride and temperature on the cephalopharyngeal skeleton of the larvae of Parasarcophaga crassipalpis in Shijiazhuan, Hebei, so as to provide evidence for forensic toxicologic entomology of sarcosaphagous flies and inference of the postmortem interval(PMI) in Shijiazhuang. Methods: Adult Parasarcophaga crassipalpis were collected. Four domestic rabbits were used in this experiment, with one receiving normal saline (control) and the others receiving morphine hydrochloride (0. 5,1. 0 and 2. 0 folds of lethal doses) via ear vein injection. Then the rabbits were hit on the head and the muscle was marked with M0, M1, M2 and M3, then the larvae were transferred to the muscle sites at 24°C, 28°C and 32°C. When larvae were laid, 10 of them were randomly sampled from each group at 12 h intervals until the beginning of the prepuparial stage. The larvae were collected every 12 h, fixed with boiling water, and stored as specimen. The scolex of the larvae was sparated and then the cephalopharyngeal skeleton was separated symmetrically, observed and photographed. Finally the indices, including the areas of different parts of cephalopharyngeal skeleton, sclerotized area, and average optical density were determined using digital image processing equipment. Results: (1) The larvae of Parasarcophaga crassipalpis grew faster with the increase of morphine hydrochloride concentration and the temperature. (2)The color of the cephalophargngeal skeleton of the larvae changed from yellow to dark brown as time went by. Meanwhile, the degree and range of chitinazation were increased, accompanied by increase of the sclerotized area. The attached larval mouth (shaped like a dot) could be observed during the third age. (3) The areas of the larval mouth hook and the larval pharyngeal sclerite increased with time. Especially, remarkable changes were observed during the fold period, and the changes reached their maximum in the third age, and then remained basically unchanged. (4) The sclerotized areas and the average optical densities of the cephalopharyngeal skeleton kept an increasing tendency till the end of the third age. The periods needed for reaching the maximal optical density and sclerotized area were greatly longer than that needed for reaching the maximal area of the cephalopharyngeal skeleton. Conclusion: The sclerotized area and the average optical density of the mouth hook and the pharyngeal sclerite of the larvae of Parasarcophaga crassipalpis keep increasing till the end of the third stage, and they are ideal indices for deducing PMI.

[Objective] To discuss the mechanism and relative problems of lumbar traction. [Method] Relevant articles and retrospect clinical data in the author's hospital were reviewed. Review relevant articles and retrospect clinical data of our hospital. [ Result ] Traction force : 40 kg + 15% ～ 20% of body weight, fineness rate reached 83.5% in 1606 patients being treated. According to course of disease, fineness rate was 90. 1% in the group of less than 3 years, 68.2% in the group of more than 3 years. [ Conclusion] Lumbosacral nerve root leave the peak of the protruding nucleus and establish a new harmonious "root-disc" relationship after traction. The pressure and tension to the nerve root reduces or disappears, meanwhile, the pain of low back and leg is alleviated or eradicated. Appropriate traction weight and correct traction body posture are key factors of good therapeutic effect.

BACKGROUND:A large number of clinical trials have found that the number of bone marrow stem cels at the femoral neck and proximal femur in patients with osteonecrosis of the femoral head is significantly reduced, accompanied by decreased activity, which causes a significant decrease in osteogenic capacity that the necrotic bone cannot be effectively repaired after absorption, leading to the colapse of the femoral head. OBJECTIVE:To probe into the early clinical efficacy of autologous peripheral blood mononuclear cel transplantation with porous core decompression for treatment of avascular necrosis of the femoral head. METHODS:Forty-five patients with avascular necrosis of the femoral head (49 hips) were enroled in this study, and underwent autologous peripheral blood mononuclear cel transplantation with porous core decompression. After treatment, pain scores, Harris hip score, scores on the satisfaction of patients were evaluated, as wel as X-ray, CT and MRI examinations. RESULTS AND CONCLUSION:Al the patients received a folow-up visit of 11-14 months, averagely (12.5±0.6) months. During the folow-up, there were no complications and serious adverse reactions. Postoperative pain scores and Harris scores were both improved significantly compared with preoperative ones (P < 0.05). At 12 months after treatment, the excelent satisfaction rate was up to 92%. Patient’s MRI low signal region accounting for a percentage of the volume of the femoral head was decreased from (40.1±7.34)% preoperatively to (20.23±5.4)% at 6 months postoperatively, and there was a significant difference (P < 0.05). These findings indicate that autologous peripheral blood mononuclear cel transplantation with porous core decompression for treatment of avascular necrosis of the femoral head has significantly clinical effects at early stage, which can obviously reduce joint pain, improve and restore hip joint function, and delay progression of disease.

Objective:Analyze the influence of hematopoietic stem cell mobilization on circulating endothelial cells (CECs) and circulating endothelial precursors (CEPs).Methods:CECs and CEPs were enumerated in 68 tumor patients and 15 healthy controls by 3-color flow cytometry. 11 cases underwent PBSC (peripheral blood stem cell) mobilization by combination chemotherapy and G-CSF (granulocyte colony-stimulating factor).Results:CECs and CEPs in tumor patients were 0.378%?0.103% and 0.059%?0.013% respectively,which were higher than that of normal controls.CECs/CEPs in peripheral blood (PB) were increased after G-CSF mobilization.Conclusion:Hematopoietic and endothelial progenitors can be mobilized into the PB by treatment with growth factor G-CSF.

Objective To construct recombinant plasmid pcDvp3 and observe the apoptosis-inducing effect of vp3 gene on human breast cancer cell line-435. Methods (1) vp3 gene was cloned into the plasmid pcDNA3.1 to form the recombinant plasmid pcDvp3. Then the nucleotides sequencing was processed. (2) 48h after transfection of pcDvp3 and pcDNA3.1 into breast cancer cell lines-435,optical microscopy, electric-microscopy, agarose electrophoresis and flow cytometry were used to verify apoptosis of tumor cells.(3)Nude mouse model of human breast cancer cells -435 was established to observe the tumor-inhibiting rate and TUNEL was adopted to identify apoptosis. Results (1) Sequence analysis justified the recombination of plasmid pcDvp3. (2) 48h after transfection into breast cancer cells-435, distinct morphological transformation and typical apoptosis bodies were observed, agarose electrophoresis of genomic DNA showed typical ladder-like pattern and flow cytometry analysis showed apoptosis peaks with the percentage of 14.42%. (3) Tumor-inhibiting rates in pcDvp3 groups were 65.52% and 68.23%, much higher than that in pcDNA3.1 group(t=4.06,P

AIM:To study the chemical constituents of the leaves of Cyclocarya Paliums(Batal.) Iljinskaja. METHODS: Chemical constituents were separated through chromatographic methods and their structures were elucidated by spectral analysis. RESULTS: Six compounds were isolated from the chloroform fraction through 75% alcohol extraction from the leaves of Cyclocarya Paliums.Their structures were elucidated as ?-Sitosterol(1),Behenic acid(2),3?,20-dihydroxyl-5?-Pregnanes(3),Kaempferol(4),?-amyrin(5),?-amyrone(6). CONCLUSION: Compounds 3?,20-Dihydroxyl-5?-Pregnanes(3),?-amyrin(5) and ?-amyrone(6) are isolated from this plant for the first time.