OBJECTIVE:To investigate the distribution and drug resistance of High-level Aminoglycoside Resistance Enterococcus spp (HLAR) in hospital infection. METHODS:The clinical specimens collected from 2004 to 2007 in our hospital were isolated by routine method. Identification of the bacteria and Antimicrobial susceptibility test were performed using automatic microbiology analysis system. RESULTS:Of the total 292 strains of Enterococcus which induced HLAR in hospital infection,Enterococcus faecalis (E.faecalis) represented 49.3% (144/292) and Enterococcus faecium (E.faecium) 41.4% (121/292). 27.4% (80/292) were from intensive care unit,17.5% (51/292) the department of nephrology and 8.9% (26/292) the neurosurgery department. The urinary system infection represented 29.8% (87/292) in hospital infection. The antimicrobial resistance of E.faecium was significantly higher than that of E.faecalis against quinolones and ?-lactamase antibiotics,but the antimicrobial resistance of E. faecalis was higher than that of E,faecium(P

0.05)) of antibiotic resistance to ATM,CAZ,FEP,IMP,AMK,and SXT were seen in different types,while remarkable(differences)(P

Aim study the characteristic of the hemolysin of Edwardsiella tarda. Methods Manner plate assay, contact hemolysis and supernatant assay were used. Result discuss the factors about affecting hemolysis activity and testing have been discussed. Conclusion there are at least two kinds of hemolysins of Edwardsiella tarda, activity of which is different with circumstance changing.

Objective This study was designed to evaluate the effect of obesity on late survival after aortic valve replacement(AVR) in patients with implantation of small aortic prosthesis(size≤21).Methods From January 1998 through December 2008,307 patients in a single center in china underwent primary AVR with smaller prosthesis survive the 30 days of surgery.Patients were defined as normal if body mass index (BM1) ＜ 24.0 kg/m2,as overweight if BMI 24.0-27.9 kg/m2,and as obesity if BMI≥28.0 kg/m2.Data of New York Heart Association(NYHA) Functional class,effective orifice area index (EOAI),left ventricular mass index(LVMI),left ventricular ejection fraction(LVEF) were got at the 3rd month(M),6th M,1 st year(Y),3rd Y,5th Y,8th Y after operation respectively.Results At multivariable analysis,obesity was independent factor of later mortality [hazard ratio (HR):1.62 ; P =0.01].Obesity group and overweight group had poor survival (P ＜0.001)and higher proportion of NYHA Function Ⅲ/Ⅳ (P ＜ 0.01) in long-term compared to normal group.EOAI were lower and LVMI were higher in obesity group and overweight group,but LVEF have no significant difference.Conclusion Obesity is associated with increased late mortality after AVR in patients with implantation of small aortic prosthesis.Obesity and overweight may also affect the NYHA function in long term.EOAI should be improved where possible as it may reduce late mortality and improve life quality in such patients.

Objective To investigate the effects of on-pump and off-pump coronary artery bypass grafting on plasma adrenomedullin (ADM) and endothelin-1 (ET-1) levels before and after operation in elderly patients. Methods Elderly patients aged 60 years and over with coronary artery bypass grafting were enrolled in the study and divided into off-pump group and on-pump group, each group had 20 cases. Blood samples were collected before surgery, immediately after operation and 6,24 hours, 3 days after operation. The plasma levels of ADM and ET-1 were dectecded by radio immunoassay. Results The levels of ADM and ET-1 were significantly increased immediately after operation in 2 groups of on-and off-pump. The levels of ET-1 in on-pump group and off-pump group were (67.59±10.18) ng/L and (58.73±9.64) ng/L respectively. The levels of ADM in on-and off-groups were (284.90±36.24) ng/L and (243.25±25.70) ng/L. This showed that in on-pump group, the plasma levels of ADM and ET-1 increased more significantly(P＜0.05). The plasma levels of ADM and ET-1 began to decline at 6 hours after operation, and continued to 24 hours after operation. On 3 days after operation, the plasma levels of ADM and ET-1 in off-pump group returned to the preoperative level, but they were still maintained at a high level in on-pump group.Conclusions For elderly patients, both on-pump and off-pump coronary artery bypass grafting can cause endothelial dysfunction, increasing plasma levels of ET-1 and ADM. But compared with the on-pump coronary artery bypass grafting, the off-pump coronary artery bypass grafting has less influence.

OBJECTIVETo explore the role of collagen degradation and scar morphology and structure in the formation of hypertrophic scar.

METHODSSDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) plus collagen substrate electrophoresis, amino acid analysis and compound staining were employed to observe the collagenase activity within hypertrophic scar, collagen degradation and the tissue morphology of the scar.

RESULTSThere exhibited deranged collagen fibres within hypertrophic scar, and large amounts of acid mucopolysaccharide closely surrounded the collagen fibres. All these led to an obvious decrease in collagenase activity and reduction of collagen degradation.

CONCLUSIONThe decrease of collagen degradation and the formation of hypertrophic scar might be closely related to the decrease in collagenase activity and the inhibiting activity of acid mucopolysaccharide on collagenase.

Adolescent ; Adult ; Child ; Cicatrix, Hypertrophic ; metabolism ; pathology ; Collagen ; metabolism ; Collagenases ; metabolism ; Female ; Humans ; Male