Objective To investigate the effects and mechanisms of extraction centipede extraction (CE) on grafting hepatocellular carcinoma (HCC) in nude mice.Methods We set up model of Bel-7404 cells heterotopic grafting HCC in nude mice,and observed the tumor growth and metastasis after intragastric administration of CE (treatment group).Immunohistochemical methods were used to detect X-chromosome linked inhibitor of apoptosis protein(XIAP),bax gene,vascular endothelial growth factor(VEGF) and angiopoietin-2(Ang-2) in tumor tissue.Results There was distinctive inhibitive effect of CE on in Bel-7404 cells heterotopic grafting HCC in nude mice.The staining cells population,staining intensity and the optical density(OD)of XIAP,VEGF and Ang-2 in the control group were higher than those in treatment group,but those of BAX were lower than that in treatment group,and there was significant difference between the 2 groups(P

Objective:To evaluate the value of DSA in diagnosis of intestinal vascular malformation.Methods:The clinical and DSA characters in 8 patients with small intestinal malformation were analyzed.There were 6 males and 2 females.All patients were underwent selective and supper selective superior mesenteric inferior mesenteric or colic artery angiographies.Results:The clinical fectures:1)the main sympton is acute lower gastrointestinal bleeding;(2)the results of conventional examination were generally negative;(3)the hemoglobin was dropped down to 4～6 g/ml in a short period.DSA feactures included:(1)artery-vein fistula;(2)markedly stainning in local small intestinal walls;(3)abnormal hyperplasia and distubance of small vessels.Conclusion:DSA is the most valuable method in diognosis of atute intestinal bleeding.

0.05). S.sobrinus strains had significantly lower initial adhesive ability to saliva coated hydroxyapatite in the absence of sucrose than S.mutans (P

砄bjective:To investigate the consistency of Streptococcus mutans and Streptococcus sobrinus isolates identified by colony morphology,biochemical tests and DNA base content analysis.Methods:46 clinical isolates (30 of S.mutans and 16 of S.sobrinus ) were identified by colony morphology,biochemical tests and determination of DNA base contents with reversed phase high performance liquid chromatography. S.mutans Ingbritt and S.sobrinus 6715 were used as the controls. Results:The DNA contents (G+C mol%) of S.mutans and S.sobrinus clinical isolates, identified by colony morphdogy and biochemical test, were 37.52 and 45.19 respectively,consistent with the expected 36～38 and 44～46 mol percent of G+C.Conclusion: S.mutans and S.sobrinus isolates,identified by colony morphology and biochemical tests ,were consistent with the DNA G+C content analysis. S.mutans and S.sobrinus can be easily and reliably identified by colony morphology and biochemical tests.

Objective To study the apoptosis in human gastric cancer cell BGC-823 induced by the components in serum of Banxia Xiexin prescription and different herbal fomulation. Method multiplication of cell BGC-823 was observed with MTT method. The genic expression of bcl-2 was tested by S-P immunohistochemistry tehnique. Results As for inhibition of cell BGC-823 multiplication, the groups of Xinkai, Kujiang and Xinku in Banxia Xiexin prescription and different herbal fomulation was dose-dependent and more sensitive than the groups of Xingan, Kugan, Ganbu and the intact prescription (P

Objective To study effect of the components in serum of Banxia Xiexin Prescription and different herbal formula on the apoptosis of cell BGC-823 and apoptosis-related gene expression. Methods The genic expression of bcl-2 and bax was tested by S-P immunohistochemistry technique. Results The apoptotic index of the groups of Xinku in Banxia Xiexin Prescription was significantly higer than that in the groups of Xingan, Kugan, Ganbu and the intact prescription (P

Objective To study the effects of single leaf Asarum on pulmonary ventilation in experimental rats with syndrome of cold fluid shooting lung. Methods Wistar rats were randomly divided into three groups including singe leaf Asarum group [fed with water extracts of singe leaf Asarum 1 mL/(kg?d)], singe leaf Asarumi Miq group [fed with water extracts of singe leaf Asarumi Miq 1 mL/(kg?d)] and normal saline group [fed with 0.9% normal saline 1 mL/(kg?d)]. After fed continually for 6 days, whole lung of rats were washed with normal saline [7 ℃, 2.5 mL/(kg?d)] and then were perfused for 30 seconds. This treatment was repeated 5 times every 5 minutes to make the model of cold fluid shooting lung. The fluid of perfusion was collected and detected. The changes of TXB2, ET, protein and phospholipids of the fluid were measured before and after lavage. Results TXB2, ET and protein in the perfusing fluid ascended obviously, while phospholipids were low in normal saline group. TXB2 and ET in singe leaf Asarum group and single leaf Asarumi Miq group were less than that in normal saline group (P

Objective To elucidate the effects of thalidomide on the expressions of basic fibroblast growth factor(bFGF) in parental and cisplatin resistant human lung adenocarcinoma cell line A 549 and A 549 DDP Methods RT PCR and lmmunohistochemistry were used to detect the bFGF mRNA and protein expression in A 549 and A 549 DDP Results bFGF mRNA expression levels and protein expressions in A 549 and A 549 DDP were all significant reduced after treatment of thalidomide (6 ?g/ml)(all P

Objective To investigate the expression difference of multidrug resistance and apoptosis related genes and its contribution to mechanisms of multidrug resistance in human lung adenocarcinoma parental cell line A 549 and cisplatin resistant cell line A 549 DDP Methods RT PCR and Immunohistochemical staining were performed to detect the mRNA expressions and protein production of multidrug resistance related protein (MRP),lung resistance protein (LRP),bcl 2,and C erbB 2 in A 549 and A 549 DDP .Results MRP,bcl 2,and C erbB 2 mRNA were expressed in A 549 ,but no LRP mRNA was detected in A 549 MRP,C erbB 2,and LRP mRNA expression levels in A 549 DDP were significant higher than those in A 549 ,otherwise,LRP mRNA was detected in A 549 DDP There was no difference of bcl 2 mRNA expressions between A 549 and A 549 DDP ( P =0 731) Bcl 2 protein expressions were strongly positive both in A 549 and in A 549 DDP MRP protein expressions in A 549 were faint positive,while C erbB 2 and LRP were negative MRP protein expressions in A 549 DDP was median positive C erbB 2 and LRP protein expressions in A 549 DDP were strongly positive Conclusions The heterogenous expression of MRP,C erbB 2,and LRP in A 549 and A 549 DDP may be associated with primary and secondary drug resistance in lung cancer

Baicalein is an important component of traditional Chinese herb, Scutellariae Radix(Huang Qin), and has various efficacies, including anti bacterial, antivirus, anti inflammation, protecting the liver function, diuresis, anti tumor, and shows good values in clinical application. It was majorly applied to acute or chronic inflammation. This article reviews developments in its pharmacological action and mechanism and provides theoretical evidences for exploiting of baicalein.