Objective To study the expression and significance of nuclear factor-kappa B(NF-κB)and proliferating cell nuclear antigen(PCNA)in uterine cervix cancer tissues.Methods The expression of NF-κB p65 and PCNA in the uterine cervix cancer tissues(n=45)and normal cervix tissues(n=30)were detected by immunohisto chemistry method.Results The expression rate of NF-κB p65 and PCNA in the uterine cervix cancer tissues were significantly higher than that in normal cervix tissues(P＜0.05).The expression of NF-κB p65 and PCNA in stage Ⅰ b,Ⅱa were significantly higher than that in stageⅠ a(P＜0.05),they were significantly higher in poorly differentiated than those in moderately differentiated,well-differentiated(P ＜ 0.05),they were significantly higher in existing lymphnode metastasis than those in non-existing lymphnode metastasis(P ＜0.05);and the expression of NF-κB p65 was positively related to PCNA in uterine cervix cancer tissues(r=0.705,P＜0.05).Conclusion The invasion,infestation and metastasis maybe dosely related to NF-κB and PCNA.