0.05 ). The effect of Sin on CaM-PDE was uncertain.It was also shown that there were cooperativity between Nit and TFP and synergism between Que and TFP. These results suggested that Nit and que may act as calmodulin antagonists and might have. different sites on calmodulin in comparison with those for TFP.

Objective Exploration trophism of protein (relative molecular quantity 67?10 3) secreted by Schwann cell to motoneuron Methods Purfication of Schwann cell secretion neurotrophic protein (67?10 3) by method of Xiao Yuzhou Spinal cord ventral motoneuron (SCVMNs) of E 12～14 d (embryonic age 12～14 d) SD rats were harvested and cultured and identified SCVMNs were cultured in different concentration of protein (67?10 3) liquid Its neurobiological activity was assayed by MTT method Results The A values was not same in different concentration of protein (67?10 3) There was a dose effect relationship between biological effect intensity and protein dose, the optimum effect protein concentration of neurotrophic protein (67?10 3) was 20 ng/ml Conclusion The protein secreted by Schwann cell can maintain SCVMNs surval in culture

Objective The extract of spinal cord tissue in morphine spared root rat was isolated and purified to look for some neurotrophic active substances. Methods Neurotrophic active substances were isolated and analysed by Sephacryl S 200 HR gel chromatography、high performance liquid chromatography(HPLC)and tissue culture,ect. Results The extract of spinal cord tissue of spared root rat could enhance the neurite growth of chick embryonal dorsal root ganglion(DRG) in vitro.There was also same effect in the extract of spinal cord tissue of morphine\|treated rat.But there was no significant difference in the extract of spinal cord tissue promoting the neurite growth between spared root rat and morphine treated rat.The extract of spinal cord tissue of morphine spared root rat had evident neurotrophic active role.The peak Ⅱ eluate and peak Ⅳ eluate obtained from the spinal cord tissue extract of morphine spared root rat through Sephacryl S 200 HR gel chromatography could promote the neurite growth of DRG.According to the analysis of SDS PAGE,the peak Ⅱ eluate showed one main protein zone with a molecular weight of 65kD and the protein composition of peak Ⅳ eluate was more complicated.The peak Ⅳ eluate of gel chromatography was then furhter isolated by HPLC.It was observed.That the peak A eluate of HPLC could promote the neurite growth of DRG. It was showed by SDS PAGE that the peak A eluate presented two main protein zones with molecular weight of 30kD and 18 kD.Conclusion\ The molecular weight of neurotrophic active substances,which were isolated from the extract of spinal cord tissue of morphine spared root rat,might be 65kD,30kD and 18kD proteins.\;[

Objective:To provide insight into the diagnosis for clinicians, the clinical characteristics, diagnosis and treatment history of 3 patients with 21-hydroxylase deficiency (21-OHD) and testicular adrenal rest tumors (TART) were analyzed.Methods:The clinical, laboratory and imaging data of 3 male patients with 21-OHD and TART, confirmed with CYP21 gene sequencing, from May 2010 to May 2021 in the First Medical Center of Chinese PLA General Hospital were analyzed retrospectively. The treatment strategy and clinical outcome were followed up.Results:All the 3 patients were first diagnosed with bilateral adrenal mass at the age of 27-42 years old. They were 145-162 cm tall. The levels of progesterone, 17-hydroxyprogesterone, and adrenocorticotropic hormone (ACTH) of the 3 patients were relatively high, and that of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) of the 3 patients were low. Testosterone level of 1 patient was significantly elevated, and that of the other 2 patients was below the lower limit of normal range. Testicular ultrasound showed heterogeneous hyperechoic masses in both testes. CT of the adrenal glands showed bilateral adrenal enlargement with mass. All 3 patients were treated with dexamethasone. After 4-96 months of follow-up, 17-hydroxyprogesterone level was kept above the median normal level. One of the patients got married and had a baby after treatment. The sizes of adrenal hyperplasia and testicular masses reduced to various degrees with the change of the testicular masses being proportional to that of adrenal hyperplasia.Conclusions:Patients with 21-OHD are prone to have TART, leading to the impaired testicular function. Early glucocorticold therapy is beneficial to the reduction of TART and restoration of testicular function.