Objective To investigate the clinical efficacy of laparoscopic splenectomy (LS) for immune thrombocytopenic purpura (ITP).Methods The clinical data of 84 patients with ITP who underwent LS at the First Affiliated Hospital of Chongqing Medical University from January 2007 to January 2014 were retrospectively analyzed.The potential prognosing indexes (gender,age,course of diseas,preoperative response to steroid,preoperative PLT,operation time,volume of intraoperative blood loss and postoperative PLT peak within 7 days) were collected and analyzed.The evaluation of surgical efficacy was done according to the American Society of Hematology 2011 evidence-based practice guideline for ITP.The complete response and partial response were defined as effective and no response as void.All of the patients were followed up by outpatient examination and telephone interview till December 2014,the follow-up information included platelet count,bleeding performance,presence of long-term complications and postoperative recurrence,etc.Measurement data with normal distribution were presented as x ± s,and skew distribution data were described as M (range).The postoperative long-term effective rate was analyzed by Kaplan-Meier method.The univariate analysis and multivariate analysis were done using the chi-square test and Logistic regression model,respectively.Results The operation time of 84 patients was (154 ±40)minutes with a median volume of intraoperative blood loss as 200 mL (10-1 000 mL).Lienculus was detected in 11 patients and resected.Seven patients had complications and recovered after symptomatic and supportive treatment.There was no perioperative death.Among the 84 patients who were followed up for an average follow-up time of 51 months (12-96 months),45 patients had complete response,18 patients had partial response and 21 had no response,without serious spontaneous visceral and intracranial hemorrhage.Eighteen partial-response patients underwent symptomatic medical therapy and 21 no-response patients took orally prednisolone,among which 13 had PLT ＞ 30 × 109/L stably.During the follow-up,only one complete-response patient died of lung cancer with systemic metastasis at 2 years after surgery.Four patients had pneumonia,1 noresponse female patient had pyothorax repeatedly for a long time,and they all recovered after symptomatic and supportive treatments.There were no serious complications and overwhelming postsplenectomy infection in other patients.The 1-,3-,5-,7-year effective rates were 82.1％,77.6％,72.5％ and 67.9％,respectively.Univariate analysis showed that preoperative response to steroid,preoperative PLT and postoperative PLT peak within 7 days were related factors affecting long-term effecacy of patients with ITP (x2=5.600,6.006,21.733,P ＜ 0.05),but gender,age,course of disease,operation time and volume of intraoperative blood loss were not related factors affecting long-term effecacy of patients with ITP (x2=0.018,2.684,0.000,0.064,0.397,P ＞ 0.05).Multivariate analysis showed that preoperative response to steroid and postoperative PLT peak within 7 days ≥300 × 109/L were independent protective factors affecting long-term efficacy (OR =5.426,19.454,95％ confidence interval:1.220-24.129,4.704-80.449,P ＜ 0.05).Conclusions LS is safe and feasible for the treatment of ITP with a high long-term effective rate.Preoperative response to steroid and postoperative PLT peak within 7 days may be the predictors of LS for ITP.

Objective:To study the protein expression of maspin in the HCC and its correlation with AFP,and to discuss its clincal significance.Methods:the protein expressions of maspin and AFP in the normal liver tissue(NT),paratumor tissue(PT)and liver tumor tissue(TT)were detected with immunohistoch-emistry and their area densities were measured.A total of30 patients with HCC and 30 patients without HCC in our Department of Hepatobiliary Surgery were as samples from March,2004 to December,2005.Results:(1)The area densities(AD)of maspin protein in the NT,PT and TT were 20 712.48?1 826.28,7 347.40?441.16,85.68?19.81 respectively,but the area densities of AFP protein were 66.00?10.70,7 005.04?335.69,13 094.00?516.32 respectively;the comparion of two was P

Objective:To explore the biological effect of maspin on VEGF(vascular endothelial growth factor,VEGF)in hepatocarcinoma cell MHCC-97 and discuss its significance.Methods:Maspin/pEFIRES-N expressing plasmid was constructed .The changes of maspin and VEGF in the levels of mRNA and protein in hepatocarcinoma cell MHCC-97 before and after the transfection and recombination were detected by RT-PCR and Western blotting.Results:The maspin/pEFIRES-N plasmid was constructed successfully,and was stably transferred into MHCC-97 cell.The expressions of maspin mRNA and protein increased in hepatocarcinoma cell MHCC-97 after the transfection of maspin/pEFIRES-N plasmid,while the expressions of VEGF mRNA and protein decreased ,as compared with those after the transfection of pEFIRES-N plasmid(P

Objective:To construct the expression vector maspin /Pcr2.1 in order to provide the evidence for the inhibitory effect of maspin gene on growth,metastasis and invasion of hepatocellular carcinoma(HCC) and to discuss its mechnism.Methods:The full length of maspin gene was cloned,and the expression vector PCR2.1 was digested by Hind111/XbaI,then the maspin gene and the PCR2.1 vector were ligated with T4 DNA ligase to be recombinant vector.The recombinant vector maspin/PCR2.1 was transfected into humman hcc MHCC-97 cell line,and the expressive changes of maspin gene were detected.Results:The recombinant plasmid was amplified in the E.coli.JM109.After the identification of redigesting by HindIII/XbaI and sequencing,the reconstructive plasmid was confirmed to contain the correct and full nucleotide sequence of maspin gene,and the mRNA and protein level of maspin gene were up-regulated in hcc MHCC-97 cell.Conclusion:The expression vector maspin/PCR2.1 is constructed successfully and can be expressed in eukaryotic cells.

AIM To investigate the effects of tetracycline on the expression of adhesive molecules E selectin and intercellular adhesion molecule 1 (ICAM 1) on human umbilical vein endothelial cells (HUVECs) stimulated by tumor necrosis factor alpha (TNF ?) or lipopolysaccharide (LPS). METHODS HUVECs were activated by TNF ? (0 5～50 ?g?L 1 ) or LPS (0 5～50 mg?L -1 ). Tetracycline (10 -6 ～10 -4 mol?L -1 ) was used to incubate HUVECs for 1 h before stimulation with TNF ? ( 1 ?g?L -1 ) or LPS ( 5 mg?L -1 ) and co incubate HUVECs with TNF ? or LPS for 4 h or 18 h. The expression of adhesive molecules on HUVECs was measured by a cellular enzyme linked immunoabsorbent assay (Cell ELISA) method. RESULTS After stimulation of HUVECs with TNF ? or LPS for 4 h induced E selectin expression, and exposure HUVECs to TNF ? or LPS for 18 h increased constitutive expression of ICAM 1 compared with control group ( P

Objective To evaluate the accuracy of the soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) and lipopolysaccharide binding protein (LBP) as diagnostic indices for neonatal serious bacterial infections (SBI). Methods A total of 171 newborns were enrolled in the study, and were classiifed into SBI group (including early-onset and late-onset), non-SBI group according to clinical manifestations, laboratory examinations and the time of disease onset. Serum sTREM-1, LBP and C-reactive protein (CRP) levels were measured. Receiver operator characteristic curve (ROC) was drawn, and the area under curve (AUC) was calculated. Each index was evaluated for the diagnostic value of neonatal SBI. Results The sTREM-1 and LBP levels were signiifcantly higher in SBI group than those in non-SBI group (P=0.000). The AUC of ROC for sTREM-1, LBP and CRP in early-onset SBI was 0.888, 0.839 and 0.706, respectively. The AUC of ROC for sTREM-1, LBP and CRP in late-onset SBI was 0.860, 0.865 and 0.705, respectively. Conclusions Both sTREM-1 and LBP are useful for the diag-nosis of neonatal SBI.

BACKGROUND:The appropriate choice of orthoses based on hemiplegic patient’s condition can improve motor function recovery in stroke patients.
OBJECTIVE:To review the motor function recovery of stroke patients with hemiplegia wearing different types of orthoses.
METHODS:Application of different types of orthoses in hemiplegic patients was reviewed through access to literature, as wel as effects of a variety of orthoses on the recovery of motor functions in hemiplegic patients.
RESULTS AND CONCLUSION:Orthoses can reduce limb muscle tension and muscle spasms, and improve motor function and overal daily living skil s in stroke patients with hemiplegia, which play a variety of roles, including stable support, fixed protection, correction of deformity and functional compensation. However, the long-term therapeutic effect of orthoses cannot be verified because of fewer cases, shorter time for therapeutic observation, and the lack of long-term fol ow-up. It is necessary to perform long-term fol ow-up for the verification of long-term efficacy of orthoses. Meanwhile, the appropriate timing for removal of orthoses needs further study.

BACKGROUND:Stem cells under appropriate conditions can be induced to differentiate into neuron-like cells. Chinese herbs can function in cellproliferation and differentiation.
OBJECTIVE:To investigate the interventional effects of traditional Chinese medicine on stem celltransplantation in the treatment of cerebrovascular diseases.
METHODS:Experimental studies concerning traditional Chinese medicine intervention for stem celltransplantation were retrieved and analyzed. Under specific conditions, stem cells can proliferate in vitro in a suspended or monolayer manner, and can be potential y induced to differentiate into neurons, oligodendrocytes and astrocytes, thereby providing basis for stem celltransplantation in the treatment of cerebrovascular diseases. We observed expressions of nestin, glial fibril ary acidic protein, and microtubule-associated protein expression for analysis of stem cellproliferation and differentiation.
RESULTS AND CONCLUSION:Baicalin or Astragalus saponin Ⅳ can stimulate stem cells to differentiate into nerve cells in vitro. Naomaitong or danshen combined with bone marrow mesenchymal stem celltransplantation can promote neurological recovery from cerebrovascular diseases in rats. Buyang Huanwu Decoction can significantly promote the proliferation of neural stem cells in the subventricular zone and neurological recovery after cerebral ischemia. Chinese herbs, such as Zhichan Decoction and Panax notoginseng, can promote survival of transplanted cells and prevent most cellapoptosis in the stem celltherapy for treatment of Parkinson’s disease. Therapy of benefiting qi and elevating yang or ginsenosides Rg1 combined with stem celltherapy can improve memory ability of dementia rats. Traditional Chinese medicine exerts a catalytic role in the stem celltherapy for cerebrovascular diseases.

Although the symptom of stable bronchiectasis is not significant, the four pathological factors including qi stagnation, sputum obstruction, blood stasis and lung-spleen qi vacuity still exist inside patients' bodies, making them in the state of both healthy and ill. This article discussed the application of the idea of preventive treatment to intervene and treat, which could prevent the emergence of new diseases and the changing of existing diseases. It could prevent vacuity evil and bandit wind, depressed qi transforming into fire, phlegm depressed in the lung and channel obstruction caused by long-term diseases. It can effectively prevent the acute attack of bronchiectasis and improve the patients' lung function and life quality.

Diabetes mellitus is a common disease of serious harmful to human health,but its pathogenesis is not entirely clear.Mitochondria are the important organelles to generate energy in eukaryocytes,and play a pivotal role in the regulation of reactive oxygen species generation,intracellular calcium homeostasis,and apoptosis signal transduction.The possible causes of mitochondrial dysfunction include oxidative stress,Ca2+ disturbances,reduction of mitochondrial biosynthesis,opening of mitochondrial permeability transition pore,mitochondrial DNA mutations,and etc..Many studies demonstrate that mitochondrial dysfunction is associated with β-cell dysfunction of type 1 diabetes mellitus and insulin resistance of type 2 diabetes mellitus.Moreover,mitochondrial dysfunction plays important roles in the development of diabetic cardiomyopathy.This present article reviewed the current status of studies on the relationship of mitochondrial dysfunction and diabetes mellitus and diabetic cardiomyopathy.It is very important to understand and study mitochondrial dysfunction and its important roles in diabetes mellitus and diabetic cardiomyopathy in order to clarify the pathogenesis of diabetes mellitus and explore new approaches of prevention and treatment for diabetes mellitus.