There are many ways to treat pathological scars.Silicon gel sheeting as a noninvasive and convenient method, are widely used in clinic.The article reviews the lituratures on theoretical research of silicon gel sheeting and its efficacy in the past 26 years, and analyzes the effectiveness of silicon gel sheeting for comparison with other methods.

Objective To observe the effect of scalp acupuncture combined with speech training on language development delay in mental retardation children. Methods 40 cases were divided into 2 groups, 20 cases in each group. The observation group was treated with scalp acupuncture combined with speech training. The control group was treated with speech training. They were assessed with the development quotient (DQ) of adaptability and language of Gesell Developmental Schedules before and 3 months after the treatment. Results DQ of adaptability and language improved after treatment in both groups (P<0.05), and improved more in the observation group than in the control group (P<0.05). Conclusion Scalp acupuncture combined with the speech training is more effective on language development delay in mental retardation children.

Adenoma ; pathology ; Carcinoma, Renal Cell ; pathology ; Diagnosis, Differential ; Female ; Humans ; Kidney Neoplasms ; pathology ; Middle Aged

Although heterologous biosynthesis of polyketide erythromycin has been successfully achieved in Escherichia coli, the titer remains at a very low level (-10 mg/L). In this study, based on genome-scale metabolic model of E. coli, in silico method flux distribution comparison analysis was used to discover novel potential targets for heterologous 6-dEB biosynthesis. Synthetic small regulatory RNAs (sRNAs) was used to experimentally test 12 down-regulated targets. The results showed that repression of each of these target genes e.g. lsrC and ackA led to significantly improve heterologous 6-dEB biosynthesis. Using co-repression of lsrC and ackA, 6-dEB titer was improved by 59.9% in shake-flask with a maximum yield of 22.8 mg/L. This study indicates that combined flux distribution comparison analysis and synthetic small regulatory RNAs is an effective strategy to improve 6-dEB production in E. coli.
Down-Regulation ; Erythromycin ; biosynthesis ; Escherichia coli ; genetics ; metabolism ; Industrial Microbiology ; methods ; RNA

Objective To discuss the influence of different concentration sulindac on pancreatic cancer cell line PANC-1 cell proliferation and apoptosis,and investigate the possible mechanism that sulindac can inhibit the Wnt/beta-catenin pathway to kill pancreatic cancer cells. Methods PANC-1 cell were divided into negative control group (added containing no sulindac DMSO)and experimental group (added sulindac with concentrations of 0.25 ,0.5 ,1 ,1.5 ,2 mM medium,respectively,name as 0.25 mM group,0.5 mM group,1.0 mM group,1.5 mM group,2.0 mM group),and treated with different time,cell proliferation inhibition ratio in each group was detected by MTT assay,cell apoptosis ratio was detected by flow cytometry,the expression ofβ-catenin mRNA and protein were detected by RT-PCR and immunocytochemistry.Results MTT results showed that sulindac can inhibit the cell proliferation of PANC-1 by a dose-and time-dependent manner.Cell apoptosis increased after sulindac treatment in different degrees,and there were statistical differences between 1.5,2.0 mMgroup and control groups (P<0.05).RT-PCR results showed that the expression ofβ-catenin mRNA decreased after the treatment of sulindac,there were statistical differences between 1.5,2.0 mMgroup and control group (P<0.05). In the 2.0mM group,the expression ofβ-catenin decreased along with the time extending (P<0.05 ).ICC results showed that sulindac inhibited the expression ofβ-catenin protein and nuclear accumulation,there were no statistical differences in 0.25 ,0.5 mM group and control group,but there were statistical differences in 1.0,1.5,2.0 mMgroup.Conclusion Sulindac could inhibit cell proliferation and facilitate apoptosis of PANC-1,this effect is dose-and time-dependent.The inhibition of Wnt/beta-catenin signal pathway may be a possible mechanism of its cytotoxicity.

Objective To report successful nursing experience of cardiac arrest caused by amniotic fluid embolism.Methods 3 pregnant women with cardiac arrest caused by amniotic fluid embolism from January to August 2012 were successfully rescued,the nursing measures included observation of labor course,mechanical ventilation,rapid establishment of intravenous injection route,anti-shock and prevention of DIC and renal failure.Results 3 cases of pregnant women were given timely rescue and pulled through.All rehabilitated and were discharged.Conclusions Amniotic fluid embolism is the most serious,dramatic,and the most difficult to control complications in obstetric department.Prevention is the key for amniotic fluid embolism,once happened,medical workers should carry out rescue work calmly and implement effective interventions.

Primary biliary cirrhosis (PBC) is an autoimmune disease with unknown causes. Most PBC patients have abnormal lipid metabolism characterized by hypercholesterolemia. Paradoxically, clinical observations and pre-experimental studies showed that the risk of hyperlipidemia associated cardiovascular event and the mortality of PBC patients were not increased. In this review we summarize the possible reasons and the underlying mechanisms.

Objective:To explore the role of sialic acid-binding immunoglobulin-like lectin-one (Siglec-1) in oxidized lowdensity lipoprotein (ox-LDL)-induced atherosclerotic inflammation. Methods: Ox-LDL was prepared by oxidization of native LDL ;different concentrations of ox-LDL were used to treat U937 cells for 48 h. Cells and supernatants were collected. The expression of Siglec-1 protein and mRNA was examined by flow cytometry (FCM) and real-time quantitative RT-PCR, respectively. The levels of MIP-1α, MCP-I and IL-8 in the supernatants were determined by ELISA. Results: Stimulation with ox-LDL significantly increased Siglec-1 protein and mRNA in U937 cells compared with the control group ( P < 0. 01). Meanwhile, the levels of MIP-Iα, MCP-I and IL-8 in the supernatants were also significantly increased in a concentration-dependent manner compared with those in the control group(P<0. 01). Conclusion: The proatherogenic effect of ox-LDL may be partly through up-regulating Siglec-1 expression in macrophages and enhancing the expression of inflammatory cytokines and chemokines. The activated macrophages recruit more macrophages and lymphocytes to the site of atherosclerotic plaques and exacerbate the inflammatory responses.

Objective: To establish a real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) method for examining the expression of sialic acid-binding immunoglobulin-like lectin-one (Siglec-1)gene in the peripheral blood mononuclear cells (PBMCs) in patients with coronary heart disease (CHD),so as to explore the relationship between Siglec-1 mRNA expression and the development/progression of CHD. Methods: Based on the fluorescent SYBR Green method, a real-time quantitative RT-PCR was set up. The expression of Siglec-1 gene in the PBMCs of 57 patients with CHD and 38 healthy controls were measured by ABI PRISM 7000 Sequence Detection Systems. The blood lipid levels were determined in all the subjects by routine biochemical examination. Results: The mean Siglec-1 mRNA copy was significantly higher in the CHD group than that in the healthy control group (mean 3.23 folds, ranging 1.28-8.11 folds, P < 0.01). The expression in the acute myocardial infarction group,stable angina and unstable angina group were 3.32(1.38-7.97),2.56(0.88-7.42),and 3.35(1.25-8.96) folds that of the control group,respectively. No significant difference was observed in the normalized Siglec-1 mRNA copy number between CHD group with normal level of serum lipids and abnormal level of serum lipids. Conclusion: A real-time quantitative RT-PCR method for detecting the expression of Siglec-1 gene in PBMCs has been successfully established. The expression of Siglec-1 is dramatically increased in PBMCs in CHD patients.

0.05 ).Fifty five patients of HoLEP group (78.6%,55/70) and 58 patients of TURP group(82.9%,58/70) were followed up at the third and sixth month postoperatively.In TURP group,6.9% of the patients(4/58) needed blood transfusion and 3.4%(2/58)developed TURS.In HoLEP group, no one needed blood transfusion or developed TURS. The differences were significant between the 2 groups ( P