Objective To study the characteristics of working memory impairment in patients with lacunar cerebral infarctions(LCI).Methods 42 patients proved by clinics were chosen,and divided into two groups:multiple and single LCI groups,versus left,right and double side infarction groups.Their working memory status were evaluated and compared with Digit Ordering Test,Corsi block task,House Visual Span Test and Stroop Digit Counting Test.All scores were analyzed statistically.Results(1)All scores of the four types of Tests in multiple LCI group were significantly lower than those in single group(P0.05).Conclusions Multiple LCI is closely related to working memory impairment.Left brain infarction may induce verbal working memory impairment,and right infarction may induce spatial working memory impairment,but object working memory impairment may related to double side of brain infarction.It suggested that in the physiological structure,different components of working memory regions had dissymmetrical effect in two sides of brain.

OBJECTIVE To investigate the prevalence of extended-spectrum ?-lactamases (ESBLs) and antibiotic resistance in Klebsiella pneumoniae isolates.METHODS ESBLs-producers were detected by CLSI phenotypic confirmatory test and susceptibilities were tested by agar dilution method.RESULTS 41.1% Of isolates were ESBLs producers in those isolates. ESBLs producers were highly resistant to piperacillin,cefazolin,cefuroxime,fluoroquinolones,chloramphenicol,SMZ-TMP and amikacin. The resistant rate to piperacillin-tazobactam,and ceftazidime was 26.3% and 34.2%. None was resistant to imipenem and meropenem in ESBLs producers. Except for carbapenems,the resistant rate to other antimicrobial agents was significantly higher in ESBLs producers than non ESBLs producers.CONCLUSIONS With resistance to most of antimicrobial agents,ESBLs-producers are highly prevalent in K. pneumoniae isolates,more attention should be paid to monitor these strains.

Objective To discuss the efficiency and safety of transjugular intrahepatic portosystemic shunt(TIPSS) in treatment of cirrhotic patients with gastroesophageal variceal bleeding.Methods52 patients who had gastroesophageal variceal bleeding were treated with TIPSS,with 11 patients of them being active bleeding,23 patients being moderate to massive ascites.Results50 patients were treated successfully with the successful rate of 96.2%.The pressure of potal dropped from 3.87?0.50kPa to 2.45?0.40kPa(P

AIM: To establish the quality standard for Gujing Tablets (Olibanum, Rhizoma Cyperi, Radix Aucklandia, etc.). METHODS: Olibanuln, Rhizoma Cyperi, Radix Aucklandia were identified by TLC, and costuslactone content in Radix Aucklandia was determined by HPLC. RESULTS: The TLC identification was highly specific and spots were clear. Costuslactone in Radix Aucklandia showed a good linear relationship. The average recovery was 99.1% (RSD=1.2% and n=5). CONCLUSION: The methods are simple, reliable and have a good reproducibility and can be used for the quality control of Gujing Tablets.

Objective To investigate the feasibility and techniques of laparoscopic biliary re-operations. Methods Laparoscopic surgery was performed in 13 patients with bile duct stones who already had undergone biliary tract operations. Results Laparoscopic choledochotomy was successfully completed in 12 cases, while a conversion to open surgery was required in 1 case. The success rate of laparoscopic surgery was 92.3% (12/13). The operation time was (101～300) min, with a mean of 155 min. No intra- or post- operative complications occurred. Conclusions Laparoscopic biliary re-operations are feasible and experiences are needed to ensure the success of surgery.

Objective To observe the morphological characteristics of oval cells in human chronic liver diseases, and determine whether there is a relationship between the number of oval cells and liver fibrotic stage. Methods Oval cells were detected in paraffin bedded liver sections of 3 normal subjects (as controls) and 29 chronic liver diseases, using histoimmunochemistry. Cells were counted if they fulfiled the morphological criteria for oval cells and showed cytoplasmic staining. Results Oval cells were not observed in normal livers. In chronic liver diseases, oval cells were located predominantly in the periportal region and fibrosis septa, characterized by an ovoid nucleus, small size, and scant cytoplasmic. The number of oval cells increased significantly ( F=22.60, P

Objective:To establish an HPLC method to determine the content of rosmarinic acid in Wuling capsules. Methods:The chromatographic column was Linksil-ODS (250 mm × 4. 6 mm,5 μm) with a gradient elution mode at the flow rate of 0. 8 ml· min-1,and the mobile phase was acetonitrile-water (32∶68) including 0. 5% acetic acid. The detection wavelength was 319 nm, the column temperature was 25 ℃ and the injection volume was 20 μl. Results: The linear range was 2-80 μg·ml-1 and the standard curve showed good linear regression within the range(r=0. 999 8). And the average recovery was 97. 07﹪ (n=6) with the inter-day precision (RSD) of 1. 35 %. Conclusion:The method is accurate and rapid with good reproducibility in the determination of ros-marinic acid in Wuling capsules, which can be used to control the quality of rosmarinic acid in Wuling capsules.

Objective To investigate the effect of ropivacaine preconditioning on the toxicity of ropivacaine to ND7/23 cells.Methods ND7/23 cells were cultured in DMEM culture medium at 37℃ in 5% CO2 incubator for 24 h at a concentration of 1 × 106/ml. The cells were randomly divided into 3 groups ( n = 3 each) ;control group (group C), ropivacaine group (group R) and ropivacaine preconditioning group (group RP). In group R, the cells were exposed to 1% ropivacaine 100 μl and incubated for 1 h. In group RP, the cells were exposed to 0.02% ropivacaine 100 μl for 15 min, after ropivacaine was washed out, 1% ropivacaine 100 μl was then added and the cells were incubated for 1 h. The cell viability was measured using CCK-8 assay and apoptosis using Annexin-V/PI staining.Results Compared with group C, the cell viability was significantly decreased, while the early apoptotic rate and late apoptotic rate were significantly increased in groups R and RP ( P ＜ 0.05). Compared with group R, the cell viability was significantly increased, while the early apoptotic rate and late apoptotic rate were significantly decreased in group RP (P ＜ 0.05) .Conclusion Ropivacaine preconditioning can protect ND7/23 cells from bupivacaine-induced cytotoxicity through inhibiting apoptosis.

Objective　To study the clinical diagnosis and treatment of juxtapapillary duodenal diverticula with biliary deseases. Methods　Eighteen duodenal diverticulum treated in our department in recent 5 years were retrospectivly analyzed, especially investigated the postcholecystectomy cases whose symptoms were continuing existence after operatoins. Articles about the surgical treatment were reviewed. Results　The total of 18 duodenal diverticulum with 17 cases of juxtapapillary duodenal diverticulum were included in this study. The ages of 12 cases were over 50 years old. Sixteen cases(88.89%) presented biliary stones. Seven cases once had performed cholecystectomy or cholecystectomy plus choledochotomy,but symptoms persisted after operations. The duodenal diverticulum were found by endoscopic retrograde cholangiopancreatography (ERCP) and hypotonic duodenography. Sixteen patients underwent surgical treatment with good effect. Conclusion　The juxtapapillary duodenal diveticula has the close relationship with biliary stones. ERCP and hypotonic duodenogrphy are the most reliable methods to get the correct diagnosis. In case of recurrent common bile duct stones after operations or persisting billiary symptoms after cholecystectomy, the coexistence of juxtapapillary duodenal diverticulum should be ruled out. The surgical treatment is only considered for the duodenal diverticulum with complication.