Objective To investigate the clinical efficacy of bleomycinum A5 on different type and site of eye hemangioma in infant. Methods 45 infant cases (23 females and 22 males) of hemangioma of eye were studied from the outpatient deparment of our hospital between 1996 and 2001. The powder of bleomycinum A5 was dissolved in 2 % procaine solution. The mixed liquid was injected uniformly into the mass of hemangioma of eye until it appeared offwhite.Results After the injection, the masses of hemangioma of eye swelled and atrophied subsequently, and the reaction disappeared within 7 days. The appearance of the local tissue turned from red to white. The hardness of it became soft several months after injection. 43 of 45 (95.5 %) patients healed after 2~5 times injection. Conclusions This method is proved effective to different type of hemangioma in infant eye, with minor damage, mild scar formation and good appearance. The side effect includes delay of development in the socket of orbit bone, local tissue necrosis and pigmentation of the skin.

Objective To explore the application value of triggered angiography non-contrast enhanced (TRANCE) technology in diagnosing lower limb arterial occlusive disease.Methods Totally 22 lower limb arterial occlusive disease patients were randomly selected,and then underwent TRANCE and DSA examinations.The arteries from the abdomen to the lower limb were divided into abdominal aorta,common iliac artery,external iliac artery,internal iliac artery,superficial femoral artery,deep femoral artery,popliteal artery,anterior tibial artery,posterior tibial artery and peroneal artery.Totally 337 sections displayed clearly were chosen to go through examinations by TRANCE and DSA.Results Of the 337 sections there were 312 ones with the same stenoses found by TRANCE and DSA,TRANCE found 16 sections with worse stenoses and 9 milder ones than by DSA.There were 153 sections with the same moderate stenoses (≥50％) displayed by TRANCE and DSA;Of the 153 sections,there were 15 ones with worse stenoses and 6 ones with milder stenoses found by TRANCE than by DSA.Kappa value of the two methods was 0.905.Conclusion TRANCE technology is a non-invasive,safe and nonradiative diagnosing method for the lower limb arterial occlusive disease.

OBJECTIVE[corrected] To understand the role of miRNA-146a in the proliferation and migration of primarily cultured rat vascular smooth muscle cells (VSMCs) and investigate the mechanisms.

METHODSPrimarily cultured rat VSMCs were transfected with a synthesized miRNA-146 inhibitor, a scramble sequence or PBS via Lipofectamine2000. Cell counting kit 8 (CCK8) and transwell assay were employed to assess the proliferation and migration of the transfected cells, and the expressions of nuclear factor-κB p65 (NF-κBp65) and proliferation cell nuclear antigen (PCNA) were detected using Western blotting.

RESULTSA 48-h transfection of the VSMCs with miRNA-146 inhibitor caused significantly lowered miRNA-146a expression as compared with that in VSMCs transfected with the scramble sequence or PBS (P<0.01), resulting also in lowered proliferative and migration ability of the cells (P<0.01). The expression levels of NF-κBp65 and PCNA were remarkably lower in cells transfected with miRNA-146 inhibitor than in the cells in the other two groups (P<0.05).

CONCLUSIONmiRNA-146a is capable of promoting the proliferation and migration of rat VMSCs probably by enhancing the expression of NF-κBp65.

Animals ; Cell Movement ; drug effects ; Cell Proliferation ; drug effects ; Male ; MicroRNAs ; antagonists & inhibitors ; genetics ; Muscle, Smooth, Vascular ; cytology ; drug effects ; metabolism ; Myocytes, Smooth Muscle ; drug effects ; metabolism ; Primary Cell Culture ; Proliferating Cell Nuclear Antigen ; genetics ; metabolism ; Rats ; Rats, Sprague-Dawley ; Transcription Factor RelA ; genetics ; metabolism ; Transfection