Objective To study the relationship between type 1 and type 2 cytokines and human acute graft-versus-host disease (aGVHD).Methods In 20 patients undergoing allogeneic peripheral blood stem cell transplantation (allo-PBSCT), plasma concentrations of interleukin-18 (IL-18), interleukin-2R (IL-2R), tumor necrosis factor-a (TNF-?) and interleukin-10 (IL-10) were measured by using enzyme-linked immunosorbent assay (ELISA).Results All the patients achieved engraftment. Eight cases deve- loped grade Ⅰ-Ⅱ aGVHD and 3 cases developed Ⅲ-Ⅳ aGVHD. The concentrations of IL-18 and IL-2R in the patients with aGVHD were significantly higher than those without aGVHD. The levels of IL-18 were correlated with the severity of aGVHD; The levels of TNF-? showed no difference between the patients with or without aGVHD; The concentrations of IL-10 in the patients with aGVHD were significantly declined but in those without aGVHD were significantly increased.Conclusion Type 1 and type 2 cytokines play an important opposite role in development of aGVHD.

OBJECTIVE To measure the compliance of laboratory personnel with different components of hand hygiene and improve their concerns for prevention.METHODS By checking and evaluating the exposing risks factors,including HIV,HBV and HCV source of infections,we found and formulated effective ways for preventing occupational disease.RESULTS The level of compliance at the end of duty was 95.0%.Pathogenic microorganisms were exclusively found on hands of laboratory personnel who wore jewelry.CONCLUSIONS Accurate evaluation and practical preventive strategies are key factors to reduce the professional exposing risks.Hand hygiene should be directed not only at healthcare workers but also at laboratory personnel.

OBJECTIVE To investigate and analyze the application of prophylactic antibiotics in perioperative period in hospital and offer the scientific basis for reasonable usage and management of antibiotics through surveying the situation.METHODS Totally 120 cases under operation were investigated prospectively from Jan to Aug in 2007.RESULTS The rate of antibiotics usage was 100% among the 120 cases.40% of the patients received single antibiotic treatment,56% and 4% received 2 or 3 kinds of antibiotics combined therapy respectively.The rate of prophylactic usage was 80.3%,the rate of therapeutic one was 19.7%;50% of the patients treated with antibiotics had the duration of postoperative prophylaxis of 7 days or more,the longest one was 30 days.the antibacterials used in turns were cephalosporins,penicillins,lincomycin,macrolides and nitroimidazole.CONCLUSIONS The duration of antibiotic used after operation is too long and the rate of combined anti-infective drugs is too high.It means that their are some problems existed in prophylactic use of antibiotics during cardiovascular surgery at perioperative period in condition of extracorporeal circulation,it is necessary that the antibiotic administration should be standardized.

The uptake of oral administered drugs primarily occurs in the small intestine,which also has the capability to metabolize drugs.Both phase Ⅰ and phase Ⅱ metabolic enzymes were expressed in the intestinal mucosa,and cytochromes P450(CYP450s) are the principle enzymes attributed to the biotransformation of drugs.CYP3A and CYP2C are the most abundant subfamilies,accounting for approximately 80% and 16% of total CYP450s in the intestine.Compared to the liver,the expression and activity of CYP450 enzymes in the intestine was susceptible to inducers or inhibitors,leading to drug-drug interaction.This article reviews the expression of CYP enzymes in small intestine and the role of the gut wall in CYP-mediated xenobiotic metabolism.Possible drug-drug interactions due to induction or inhibition of CYP enzymes in the small intestine are also addressed.

Objective To study the association of serum transforming growth factor beta 1 (TGF-?1) and insulin-like growth factor 1 (IGF-1) with left ventricular remodeling in essential hypertension (EH) by measuring the changes of their serum levels in patients with essential hypertension (EH).Methods RIA and ELISA were used to detect serum TGF-?_1, IGF-1, AngⅡ, ALD and the left ventricular mass index (LVMI) was calculated in 59 patients with EH and in 29 normal subjects. Results The level of TGF-?1 in EH group was lower than that in normal subjects (P

Pegylated interferon (PEG-IFN) combined with ribavirin (PR regimen) has been used in China for more than 10 years.With the development of direct-acting antiviral agents (DAAs),the therapeutic regimens for hepatitis C has become more and more diverse.In the regimen of DAAs combined with PR regimen,IFN helps to benefit the long-term prognosis of patients with chronic hepatitis C (CHC),and the addition of DAAs may improve therapeutic effect,shorten the course of treatment,and further improve the current status of treatment of CHC.This article introduces the advantages and disadvantages of PR regimen,DAAs combined with PR regimen,and oral regimen with DAAs,in order to investigate the therapeutic regimens suitable for different CHC patients and provide a reference for clinical practice.

To explore the anticancer effect of curcumin on human B cell non-Hodgkin's lymphoma and compare its effects on human B cell non-Hodgkin's lymphoma cells and normal peripheral blood mononuclear cells (NPBMNCs). MTT assay was used to study the effect of curcumin on the growth of Raji cells and NPBMNCs. The effect of curcumin on the apoptosis of Raji cells and NPBMNC were studied by flow cytometry and TDT-mediated dUTP nick and labeling (TUNEL). The effect of curcumin on the cell cycle of Raji cells were examined by propidium iodide staining flow cytometry. The results showed that curcumin strongly inhibited proliferation of Raji cells, 24 h IC50 for Raji cells was 22.8 +/- 1.82 micromol/L and curcumin induced Raji cell apoptosis in a time- and dose-dependent manner. Raji cells treated with curcumin showed G0/G1 or G2/M phase increase and S phase decrease. However, curcumin did not demonstrate apparent proliferation inhibition and apoptosis induction in NPBMNCs. It was concluded that curcumin is able to inhibit the proliferation of Raji cells by regulating the cell cycle and inducing the cell apoptosis. Morever, curcumin has low toxicity on NPBMNCs but can selectively induce apoptosis in Raji cells.
Antineoplastic Agents, Phytogenic/*pharmacology ; Apoptosis/*drug effects ; Cell Cycle/drug effects ; Cell Proliferation/drug effects ; Curcumin/*pharmacology ; Dose-Response Relationship, Drug ; Lymphoma, B-Cell/*pathology ; Tumor Cells, Cultured

The anticancer activity of trichostain A (TSA) on human B cell non-Hodgkin's lymphoma and its mechanism were explored. The effect of TSA on the growth of Raji cells and normal peripheral blood mononuclear cells (NPBMNC) was studied by MTT assay. The effect of TSA on the apoptosis of Raji cells and NPBMNC was studied by flow cytometry and TDT-mediated dUTP nick end labeling (TUNEL). The effect of TSA on the cell cycle of Raji cells was studied by propidium iodide method. The results showed that TSA potently inhibited proliferation of Raji cells at microgram concentrations and induced apoptosis of Raji cells in a time- and concentration-dependent manner. Treatment with TSA induced accumulation of cells in G0/G1 or G2/M and a concomitant decrease of cell population in S phase. However, NPBMNC was less sensitive to the cytotoxic effect of TSA than Raji cells. It was concluded that TSA may inhibit the proliferation of Raji cells by regulating the cell cycle and inducing the cell apoptosis. Moreover, TSA demonstrates low toxicity in NPBMNC but selectively induces apoptosis of Raji cells.

Objective To evaluate the effects of a comprehensive intervention including regular aerobic exercise and health education on knowledge,attitude,belief,practice (KAP) scores,body mass index (BMI),waist circumferences,blood pressure,serum glucose and lipid levels in middle-aged and elderly people with overweight and obesity.Methods Three resident communities were chosen randomly from Wangdingdi community in Nankai district in Tianjin.Totally 125 overweight people were involved in this study,62 and 63 people were randomly assigned to intervention group and control group respectively.The members of intervention group were asked to participate in aerobic exercise and health education program 3 times per week within 6 months.And control group members received no intervention.Then to evaluate the effects of intervention of regular aerobic exercise and health education by physical examination and questionnaire.Comparison between intervention group and control group was performed using a repeatedmeasures analysis of variance.Results The comprehensive intervention resulted in significant improvement of the KAP scores [13.82 (95％CI:12.42-15.23)] and high-density lipoprotein cholesterol [0.22(95％ CI:0.17-0.28) mmol/L] and reductions in intervention group in body mass index [-0.47 (95％ CI:-0.58-0.36) kg/m2],waist circumference [-4.00 (95％ CI:-5.01--2.99) cm],systolic blood pressure [-11.69 (95％ CI:-13.84--9.55) mmHg] (1 mmHg=0.133 kPa),fasting plasma glucose [-1.21 (95％ CI:-1.55--0.88) mmol/L],triglyceride [-0.06 (95％ CI:-0.25-0.13) mmol/L],low-density lipoprotein cholesterol [-0.48 (95％ CI:-0.62--0.34) mmol/L].Conclusion Regular aerobic exercise and health education program in community can successfully reduce body mass index,waist circumference and some other risk factors related to overweight and obesity.It is effective to improve awareness,physical fitness,change lifestyle for middle-aged and elderly people in the community.

Objective To investigate the effect of propofol post?conditioning on oxygen?glucose deprivation and restoration ( OGD∕R)?induced abnormal cell cycle activation in hippocampal neurons of rats. Methods Primarily cultured hippocampal neurons obtained from fetal Wistar rats were cultured for 7 days and seeded in culture wells (100 μl∕well) or in culture flasks (3 ml∕flask) at a density of 5×105cells∕ml. The neurons were divided into 3 groups ( n=24 each) using a random number table: control group ( group C); group OGD∕R; propofol post?conditioning group (group PP). The neurons were subjected to oxygen?glucose deprivation for 1 h followed by restoration of oxygen?gulcose supply for 24 h. Propofol 1.2μg∕ml was added immediately after onset of oxygen?glucose restoration, and the neurons were incubated for 2 h in group PP. At 24 h of oxygen?glucose restoration, cells were collected for measurement of the cell viability by methyl thiazolyl tetrazolium assay, and the mitochondrial membrane potential ( MMP ) , intracellular Ca2+concentration ( [ Ca2+] i ) and distribution of cell cycle were determined using flow cytometry. Results Compared with group C, the cell viability and MMP were significantly decreased, [ Ca2+] i was signifi? cantly increased, the proportion of the cells in G0∕G1 phase was significantly decreased, and the proportion of the cells in S and G2∕M phases was significantly increased in OGD∕R and PP groups (P<0.05). Com?pared with group OGD∕R, the cell viability and MMP were significantly increased, [ Ca2+] i was significant?ly decreased, the proportion of the cells in G0∕G1 phase was significantly increased, and the proportion of the cells in S and G2∕M phases was significantly decreased in group PP (P<0.05). Conclusion The mechanism by which propofol post?conditioning reduces OGD∕R?induced apoptosis in hippocampal neurons of rats is associated with inhibition of abnormal cell cycle activation.