Objective To study the effects of mobile telephone microwave radiation on learning and memory of young and senile mice. Methods The young(5 months old) and senile(10 months old) male Kunming mice were randomly divided into 2 groups respectively, the control and exposed. The young and senile mice were settled in the environment of the mobile telephone signal shoots station (3 V/m) and exposed to the mobile telephone conversation radiation with two mobile telephones for 5 h/d for 50 consecutive days. The control group was in the environment without microwave radiation (0 V/m). The capability of learning and memory of each group were determined by Morris maze test(the time of finding the flat roof:the latency), the activity of acetylcholinesterase (AchE) in the hippocampus and temporal lobe were measured by spectrophotometry. Results With the increase of training time, no significant decrease of the latency was seen in each group. Compared with the senile control group, the activity of AchE in the hippocampus and temporal lobe of the young control group and the senile radiation group were lower(P

The integration of the habitat processing and the processing of Chinese herbal medicine is an effective way to ensure the quality of Chinese medicine decoction pieces. This paper analyzes the historical origins, generation and practice, and modern research results of “integration”. It is believed that “integration” is a historical inevitable trend of industry development, and it can effectively solve the problems of the confusion in habitat processing and the difficulty in ensuring the quality of the pieces. It has the positive meaning of being able to clarify product attributes and regulate the management of pieces, abandon the repeated links and simultaneously improve the quality of the pieces and the benefits of the enterprise, and change the status of scattered processing of Chinese herbal medicines in the field, ensure the cleanliness of the production environment and comprehensively improve mechanized production. From the current research results, “integration” can simplify the processing technology, retain more active ingredients and improve the efficacy of drugs, but some Chinese herbal medicines are not suitable for integration. It is suggested to further strengthen the basic research of the integrated decoction pieces in the future, further improve the standardization system of integrated decoction pieces, carry out top-level design of “integration” and cooperate with each other to ensure the healthy and orderly development of “integration”.

The habitat processing and the processing of Chinese herbal medicine (CHM) are the two closely linked to the link in Chinese traditional medicine industrial chain. This paper analyzes the internal relationship among the three aspects in the historical evolution from the historical origin, the professional differentiation, and the development; The research status of the five aspects, such as the integration from their industry definition background, proposal, significance, and current progress of the integration of the habitat processing and the processing of CHM. It is believed that the integration accords with the development trend of history, which is a good way to solve the problem of the quality of Chinese materia medica. Suggestion is given that we should pay more attention to the basic research, in-depth the industry research, the promotion of the industry legislation, and through the supervision of the industry, to the ensurence of the healthy and orderly development of the integration.

Objective: To observe the curative effect of TCM syndrome differentiation and treatment for novel coronavirus pneumonia (novel coronavirus pneumonia, NCP) patients and the preventive effect for Chinese medical staff. Methods: A total of 62 NCP suspected patients admitted in 2020 were treated with TCM syndrome differentiation and treatment, as well as our hospital medical staff with No.1-4 hospital prescription. Results: After taking traditional Chinese medicine, 16 out of 25 NCP suspected patients with phlegm heat stagnating lung syndrome were discharged to home for isolation observation, 4 patients hospitalized for observation, and 5 patients confirmed with NCP. For 15 patients with phlegm dampness accumulating lung syndrome, 7 patients were discharged to home for isolation observation, 3 patients were hospitalized for observation and 5 patients have been confirmed. For 18 patients with spleen stomach disharmony syndrome, 15 patients were discharged to home for isolation observation, 1 patient was hospitalized for observation and 2 patients have been confirmed. For 4 patients with Qi deficiency and dampness stagnation syndrome were discharged to home for isolation observation, 1 patient was hospitalized for observation, and two have been confirmed. The duration of taking traditional Chinese medicine was 1 to 20 days from admission to be discharged. The doctors and nurses who took the prescription of TCM for 12 to 15 days have been prevented from NCP infection. Conclusions The clinical effect and the preventive effect of TCM syndrome differentiation and treatment for NCP have been proved to be satisfactory. TCM can go into the primary hospital for treatment and prevention on NCP.

OBJECTIVE: To study the load capacity and slow releasing kinetics of chloramphenicol in poly(DMAM-co-AA) hydrogel based on N,N-dimethyl acrylamide (DMAM) and acrylic acid (AA). METHODS: The dry hydrogel was soaked in chloramphenicol solution, fully saturated and swelled. The load capacity of chloramphenicol in the swelled hydrogel was investigated. The dry gel loaded chloramphenicol was put into the different solution and constantly stirred. At the same time, the release rate of chloramphenicol was studied. RESULTS: When themole ratio of reaction monomers (DMAM/AA) and concentration of chloramphenicol solution were increased, the load capacityof chloramphenicol was increased. The release rate of chloramphenicol was increased with temperature rising. The release rate of chloramphenicolin acidic or alkaline medium was larger than that in neutral medium. CONCLUSION: The load capacity of chloramphenicol in poly(DMAM-co-AA) hydrogel is increased with the solution concentration of chloramphenicol increasing. The release of chloramphenicolin various hydrogels is completed in the range of 8 to 12 h, and the total release rate of chloramphenicol could reach about 90%.

OBJECTIVE: To investigate the effect of bone cement dispersion and distribution on the clinical effect and the degree of pain reduction of percutaneous vertebroplasty(PVP) in the treatment of osteoporosis spinal fracture. METHODS: A retrospective analysis was made of 130 cases of osteoporotic spinal fractures admitted from August 2016 to April 2018, of which 114 cases were followed up completely. The VAS score, Oswestry disability index(ODI), kyphosis angle(Cobb angle), anterior column height and complications were analyzed. RESULTS: Finally, 114 cases were included. The grade of bone cement dispersion was 42 cases (36.8%) in grade 1, including 14 males and 28 females, with an average age of (73.43 ±7.91) years. There were 36 cases of grade 2(31.6%), including 10 males and 26 females with an average age of (71.22 ±8.06) years, and 36 cases with grade 3 (31.6%), including 9 males and 27 females, with an average age of (74.81 ±6.91) years. There were no significant differences in preoperative general data among the three groups (>0.05). The VAS, ODI score of grade 1, grade 2 and grade 3 of bone cement dispersion and distribution were significantly lower than those of preoperative follow-up(<0.05) in the follow-up period of 1, 1, 3 and 6 months after operation(<0.05). There were no significant differences between the three groups in using single factor ANOVA at the same time point of postoperative follow-up(>0.05). In terms of bone cement leakage, 15 cases(41.7%) were in grade 2 of diffusion distribution, 23 cases(63.9%) in grade 3 more than 8 cases(19%) in grade 1 of diffusion distribution, exhibiting a significant difference among the three groups (<0.05). The diffusive distribution in grade 2(7, 19.4%) and grade 3(8, 22.2%) less than that in grade 1(18, 42.9%)(<0.05). Bone cement dispersion and distribution with grade 2 and 3 level could effectively improve the kyphosis deformity and reduce the loss of anterior column height in the injured vertebrae compared with that with grade 1, showing a statistical difference among the three groups(<0.05). CONCLUSIONS Bone cement dispersion and distribution with any grades in PVP are effective in relieving pain, and bone cement dispersion and distribution with grade 2 could not only relieve the pain of patients, but also correct the kyphosis angle of the injured vertebrae. The height of the anterior column of the injured vertebrae and the risk of cement leakage and non-operative vertebral body fracture is relatively low.
Aged ; Aged, 80 and over ; Bone Cements ; Female ; Fractures, Compression ; Humans ; Male ; Middle Aged ; Osteoporotic Fractures ; surgery ; Retrospective Studies ; Spinal Fractures ; Treatment Outcome ; Vertebroplasty

Objective To detect the expression of survivin mRNA in cervical cancer cell lines using molecular beacon imaging technology. Methods Human cervical cancer cells (HeLa and SiHa) and human fetal lung fibroblast HFL-I were cultured in vitro. After adding 100 nmol/L survivin mRNA molecular beacon, the fluorescent signals were observed under fluorescent microscope. The expressions of survivin in cervical cancer cells and HFL-I cell were examined by immunocytochemical streptravidin-biothin peroxidase (SP) assay at the same time. Results Two kinds of survivin mRNA molecular beacon, with different color fluorescence, had strong fluorescent signal in cervical cancer cell lines, and the signal in SiHa cell line was stronger, but these signals were not found in HFL-I ; Immunocytochemical staining of positive survivin was located in the cytoplasm of cervical cancer cell lines HeLa and SiHa, whereas, no expression of survivin was detected in HFL-I cell line. Conclusion The technology of molecular beacon imaging can be used to detect the expression of survivin mRNA in viable cells successfully, and may provide a new approach to the diagnosis of early stage cervical cancer and the following-up in the clinic.

Objective To observe the effects of neuromuscular activation (Neurac) training on balance and walking in children with hemiplegic cerebral palsy. Methods 30 children with hemiplegic cerebral palsy from March to October, 2015 were divided into observation group (n=15) and control group (n=15). Both groups received routine rehabilitation, while the observation group received 30-minute Neurac training in addition, 5 times a week, for 3 months. They were assessed with D and E domains of Gross Motor Function Measure 88 (GM-FM-88), balance of Fugl-Meyer Assessment and footprints analysis before and after treatment. Results The scores of D and E domains of GMFM-88 and balance of Fugl-Meyer Assessment, and the step length, step width and velocity improved in both groups after training (t>7.31, P<0.001), especially in the observation group (t>2.08, P<0.05). Conclusion Neuroac training can further promote the recovery of gross motor function, balance and walking in children with hemiplegic cerebral palsy.

OBJECTIVE： To investigate the characteristics and regularity of hepatotoxicity induced by quinolones， and to provide reference for clinical use of drug safely. METHODS： Using “quinolone” “floxacin” “hepatotoxicity” “hepatic injury”as retrieval words， relevant literatures about hepatotoxicity induced by quinolones were retrieved from domestic and foreign databases as CNKI， Wanfang， VIP， PubMed （during database establishment to 31th， Dec. 2017）. Those literatures were summarized and analyzed. RESULTS： A total of 59 valid literatures were collected， including 61 cases of hepatotoxicity induced by quinolones， 8 types of drugs as ciprofloxacin， moxifloxacin， ofloxacin， lomefloxacin， norfloxacin， levofloxacin， gatifloxacin and enoxacin. Ciprofloxacin， levofloxacin， moxifloxacin and ofloxacin were the most common drugs that caused hepatotoxicity， involving 19， 13， 11， 7 cases， respectively； accumulative constitute ratio was 81.97％. The ratio of male to female was 1.54 ∶ 1， and hepatotoxicity always happened at the age of 61 to 80 （30 cases， 49.18％）. Primary diseases of 46 cases were single disease （75.41％）， and mainly were infection of respiratory system and urogenital system. There were 15 cases of combined disease （24.59％）. Thirty-one cases used quinolones alone， most of which was ciprofloxacin. There were 30 cases of drug combination. Thirty-four cases were given drug intravenously and mainly were domestic cases. The hepatotoxicity first occurred within 10 minutes after administration and at the latest 8 weeks after administration. Forty-nine patients suffered from hepatotoxicity within 10 days after medication， accounting for 80.33％. Besides general fatigue， nausea and vomiting， clinical symptoms also included abnormal elevation of alanine aminotransferase， aspartate aminotransferase and total bilirubin，etc. Fifty-four patients were improved after withdrawal or symptomatic treatment， while 7 patients died. The results of causality evaluation of drug-induced hepatic injury showed that there were 4 probably association cases， 45 likely association cases and 12 possible association cases. CONCLUSIONS： The hepatotoxicity caused by quinolones is related to drug variety， patient’s age， primary disease， drug combination and route of administration， and mostly occurs within 10 days after administration. Great importance should be attached to patient’s liver function indexes， strengthen medication monitoring， and carefully combined use of drugs.

Objective:To investigate the incidence, drug sensitivity and drug resistance characteristies, and theraputic effect of staphylococcal peritoneal dialysis-associated peritonitis (PDAP), aim to provide clinical evidences for standardizing treatment therapy of staphylococcal PDAP. Methods:Clinical data of PDAP patients admitted to the Second Hospital of Jilin University, the First Hospital of Jilin University-the Eastern Division, Jilin Central Hospital and Jilin First Automobile Work General Hospital during January 1, 2013 and December 31, 2019 were retrospectively collected. The results of etiology, drug sensitivity and drug resistance of staphylococcal PDAP patients were collected. According to the pathogenic bacteria, patients were divided into staphylococcus aureus group ( n=48) and coagulase-negative staphylococcus group ( n=232). According to the results of methicillin resistance, patients were divided into drug-resistant group ( n=71) and drug-sensitive group ( n=30). The prognosis of antibiotic therapy in each group were compared. Poisson regression was used to test the changing trend of the incidence of staphylococcal PDAP. The changes of drug sensitivity and drug resistance of staphylococcus were compared between 2013 and 2019 by linear trend χ2 test. Results:A total of 1 085 cases of PDAP occurred in 625 patients were screened, and 280 cases of staphylococcal PDAP were finally included. The incidences of staphylococcal PDAP, staphylococcus aureus PDAP and coagulase-negative staphylococcal PDAP were 0.063 times per patient year, 0.010 times per patient year and 0.053 times per patient year respectively. In addition, the incidence of PDAP caused by staphylococcus, staphylococcus aureus and coagulase-negative staphylococcus decreased year by year (all P<0.05). With the change of years, the sensitivity rate of staphylococcus to rifampicin increased, while the sensitivity rate of staphylococcus to moxifloxacin decreased (both P<0.05). The drug resistance rate of staphylococcus to levofloxacin increased ( P<0.05). The staphylococcus aureus group was more prone to refractory PDAP and catheter removal than that in coagulase-negative staphylococcus group, and the recurrence rate was higher than that in coagulase-negative staphylococcus group (all P<0.05). The proportion of vancomycin used during the whole course of antibiotic therapy in drug-resistant group was higher than that in drug-sensitive group ( P<0.05). Conclusions:The incidence of staphylococcal PDAP decreases year by year, and the drug sensitivity characteristics of staphylococcus also change. The therapeutic outcomes of staphylococcus aureus PDAP are worse than that of coagulase-negative staphylococcus.