Objective:To study the effect of RNA interference(RNAi) on PAR1 gene mRNA expression and the invasive petential of human colon cancer cell lines.Methods:Chemically synthesizeddouble stranded RNA(dsRNA) targeting PAR1 was transfect- ed into human colon cancer cell Lovo using SiPORT Lipid.The transfection eficiency was observed under fluorescence confocal microscopy.Expression of PAR1 mRNA and protein was detected by reverse transcription polymerase chain reaction(RT-PCR) and Western blot.Cell penetrate matrigel capacity were determined by invitro experiment.Results:PAR1-siRNA effectively in- hibited PAR1 mRNA and protion expression(P

Circulating tumor cells (CTCs)are special kind of tumor cells in the peripheral blood of patient with tumor.Now CTCs detection has been used in the survival time prediction,post-operational recu-rrence detection,individualized treatment and other aspects in the patients with gastric cancer.As the research going,CTCs will provide new help for the comprehensive treatment of gastric cancer.

Objective: To analyze the epidemiological characteristics and clinical features of the patients with 2019-nCoV infection, so as to provide basis for clinical diagnosis. Methods: The epidemiology, clinical symptoms, laboratory and radiologic data of 23 patients with 2019-nCoV infection admitted to the Fifth People's Hospital of Xinyang City from January 22,2020 to January 29, 2020 were retrospectively analyzed. Results: The 23 patients with 2019 nCov infection consisted of 15 men and 8 women, and the median age was 46.0 (40.5, 52.0) years (27-80 years); 9 of them had basic disease (39%), including hypertension (17%), cardiovascular diseases (17%), diabetes (9%), hypothyroidism (4%) and old tuberculosis (4%). All the 23 patients had contact history in Wuhan area or with confirmed infections. Clinical symptoms included: fever (100%), cough (70%), expectoration (43%), myalgia (26%), headache (17%) and dyspnea (17%), and the less common symptoms were diarrhea (4.3%). Blood routine test: white blood cells (WBC) < 4×10⁹/L in 11 cases (48%), (4-10)×10⁹/L in 10 cases (43%), >10 × 109/L in 2 cases (9%); lymphocytopenia in 13 cases (56%). All 23 patients had different degrees of infective lesions in chest CT examination, with 9 cases (39%) on one side and 14 cases (61%) on both sides. Classification: 19 mild cases, 4 severe cases, no critical or death case. Complications included acute respiratory distress syndrome [4 (17%)]. No case was reported with the damage of liver or kidney function and with secondary infection. Conclusions Epidemic history of contact, fever, pneumonia signs of chest CT, normal or decreased count of WBC and lymphocytopenia are the clinical basis for diagnosis of the disease. However, at present, the treatment of patients has not been completed, the effective treatment strategy and final prognosis are not clear.

Critical care medicine is a relatively young, fast-growing discipline, but it also bears the burden of heavy life. In the past 10 years, critical care medicine has made rapid progress. It has been growing to be mature and complete, its environment and equipment have been improved, and the technical ability has been continuously upgraded. However, the rapid improvement and the diversification of management modes have also brought about some concerns about medical quality. Xinyang Central Hospital is a large prefecture-grade tertiary-class A general hospital in Henan Province. As the director of the department of critical care medicine and the discipline leader of critical care medicine in Xinyang City, the author shared his opinion about how to establish a "standardized, institutionalized and procedural" management model from the perspective of department management in order to ensure medical quality and safety. We should "start from the quality control of critical care medicine, implement the concept of critical care through ward rounds, supervise the quality from the self-examination and self-correction of adverse events", in order to establish a management system for critical care patients and lay a foundation for improving medical quality and sustainable developing of critical care medicine.
China ; Critical Care/standards* ; Hospitals, General ; Humans ; Quality of Health Care ; Tertiary Care Centers

Metastasis is the essential character of biological behaviors in malignancies,which is also the main reason for the death of patients with tumor. So it is very important to research the metastasis for the pre-vention and treatment of carcinomas. Rectal cancer is one of the most common gastrointestinal cancer,and its prognosis is bad. lymphatic metastasis is one important factor in the determination of colon cancer' s progno-sis. More than 50% patients are found lymphatic metastasis when taking operation. The ralationship of lym-phatic metastasis and prognosis is more important than the original tumor's invasiving. So it is meaningful to study the mechanism of tumor metastasis, and to provide a scientific approach to the prediction of tumor me-tastasis, the precise assessment of prognosis and the treatment of tumor metastasis.

Objective: To report the first case of a neonatal pneumonia with 2019-nCoV infection, and the experience of successfully diagnosis and treatment in late pregnancy woman with novel coronavirus pneumonia (critical type) in Xinyang city. Methods: The successfully diagnosis and treatment of a woman with 38 weeks singleton pregnancy complicated with novel coronavirus pneumonia (critical type), and a case of neonatal pneumonia with 2019-nCoV infection were retrospectively analyzed. Results: A single male was successfully delivered at 38-week gestation of his mother by cesarean section under third level protection in operation room. The delivery woman was diagnosed with 2019-nCoV infection at day 2 of delivery. Dyspnea and severe hypoxemia soon developed, and invasive mechanical ventilation was given. After active rescue and treatment, the delivery woman had been taken off line successfully and the condition was stable. Pharyngeal swab specimen of the neonate was sent for examination 3 days after birth, and was positive for novel coronavirus nucleic acid by fluorescence reverse transcript polymerase chain reaction. Conclusion 2019-nCoV may be transmitted vertically from mother to child.

Objective To construct anti-astrocyte elevated gene-1(AEG-1)single-chain variable antibody (V23)prokaryotic ex-pression vector,and to conduct the protein purification and immunological activity detection.Methods The Primer5 software was applied to design the primers aiming at the gene sequence of the antibody anti-AEG-1 single-chain variable region for constructing the prokaryotic expression plasmid of PRsetC/V23.After the enzyme digestion by the restriction enzyme Pst1 and correct DNA se-quencing,the prokaryotic expression plasmid was led to E.coli BL21 ,the prokaryotic expression engineering strain containing the V23 gene was constructed.After the induction with IPTG,the interest protein was purified by the magnetic beads with the HIS tag,and the content of interest protein was determined by the SDS-PAGE electrophoresis.Western blot and ELISA were adopted to detect the immune activity of the nti-AEG-1 single-chain variable region antibody.Results For the constructed prokaryotic expres-sion plasmid PRsetC/V23,the single enzyme digestion and sequencing analysis displayed that the constructed V23 gene was com-pletely consistent to the designing sequences.After IPTG induction,SDS-PAGE electrophoresis showed an apparent protein band at 31×103 ,the Western blot detection showed a specific AEG-1 response band at 80 ×103 ,the ELISA test showed the positive re-sults.Conclusion The PRsetC/V23 prokaryotic expression plasmid and the V23 prokaryotic expression engineering strain are suc-cessfully constructed,this engineering strain can express anti-AEG-1 single-chain variable region antibody protein,and the protein has good immune activity.

Vietnam,as an important country along the "one belt and one road",is closely related to China.Chinese traditional medicine has entered Vietnam and gained strong support from policy and public recognition.It has a good foundation for development and a bright future.Based on the current situation of traditional Chinese medicine exchanges between China and Vietnam and Vietnam's basic national conditions,the author puts forward that exploring new cooperative models through multi-level cooperative projects,which relys on the role of the Chinese Chamber of Commerce,and adheres to the complementary promotion between trade in services and trade in goods.They are the feasible ways for Chinese medicine to develop in Vietnam.The development of Chinese medicine in Vietnam needs the adherance to the four principles of bidirectional positioning,suiting measures to local conditions,standardizing standards and risk prevention.

In recent years, the cooperation between China and the Republic of Lebanon (hereinafter referred to as "Lebanon" ) in the field of Traditional Chinese Medicine (TCM) service trade has become increasingly urgent. At present, TCM in Lebanon is facing challenges such as the westernized medical environment, the imperfect system of TCM doctors’ recognition, the strict drug access conditions, the imperfect industrial chain of Chinese medical products, and the significant impact of cultural and regional differences on the market demand for Chinese medicine. It is suggested that health care products should be taken as the breakthrough point to develop service trade, enterprises should be attracted to settle in Lebanon from the national level to produce Chinese medicine preparations on the spot, and pay attention to high-level contact, establish TCM hospitals, and deepen cultural exchanges, so as to promote the development of Chinese medicine in the Lebanon.

Pediatric urinary incontinence (PUI) is common in clinical practice and seriously affects the quality of life as well as physical and mental health of patients. PUI is a multi-factorial related abnormality, very complex in etiology and types. The occurrence of PUI is mostly associated with abnormal vesicourethral function. Urodynamic examination (UDS) is the golden standard to assess voiding function and diagnose the type of bladder and urethral function in children with PUI. UDS of PUI is of great clinical value in determining the cause, making treatment protocol as well as evaluating the therapeutic response. However, UDS in children has not been popularized in China, which seriously affects the diagnosis and treatment of PUI. This article reviews the research progress in the clinical application of UDS in the evaluation of PUI, in order to provide reference for improving the diagnosis and treatment of this disease.