Objective To investigate the effect of acupuncture at the primary points of the three yin meridians of the foot on blood pressure in an animal model of high/low blood pressure and explore the specific effect of the meridian points-the primary points of the three yin meridians of the foot.Methods The study included the parts of hypertension and hypotenstion. Both parts were allocated to the primary points of the three yin meridians of the foot, non-acupoint, model and blank groups, six rats each. Except the model and blank groups, all the groups were given acupuncture treatment for seven consecutive days. Blood pressure was measured after the completion of treatment. Five consecutive measurements were made in each rat. Mean values of blood pressure were taken.Results For the part of hypertension, blood pressure at each time point was higher in the acupuncture groups than in the blank control group and lower than in the model group, and there were statistically significant differences (P<0.05), indicating that acupuncture at the primary points of the three yin meridians of the foot has a hypotensive effect. The hypotensive effects of Taichong and Taixi were better than those of Taibai and the non-acupoint (P<0.05). The hypotensive effects of Taichong and Taixi were equal (P>0.05). The hypotensive effect of Taibai was equal to that of the non-acupoint (P>0.05). For the part of hypotension, blood pressure at each time point was lower in the acupuncture groups than in the blank control group and higher than in the model group, and there were statistically significant differences (P<0.05), indicating that acupuncture at the primary points of the three yin meridians of the foot has a pressor effect. The pressor effect was worse in the the non-acupoint group than in the primary points of the three yin meridians of the foot group (P<0.05). The pressor effects of Taibai and Taixi were equal but worse than that of Taichong (P<0.05).Conclusions Acupuncture at the primary points of the three yin meridians of the foot has a specific bidirectional regulating effect on blood pressure. Of them, point Taichong produces a best bidirectional regulating effect on blood pressure.

Objective To sum up the experience on diagnosis and treatment of Hashimoto disease and its concurrent thyropathy. Methods Clinical data were analyzed on 52 cases of Hashimoto disease(HD) surgically found to have concurrent thyropathy from Jan 1984 to Jun 2001 out of 138 HD cases undergoing surgery. Results Thyroid nodules were found in all 52 HD cases, among which 17 were with thyroid cancer, 2 with malignant lymphoma, 5 with thyroid adenoma, 2 with hyperthyroidism, and 26 with nodular goiter. Thyroidectomy of different extent was performed. Postoperatively all cases were given thyroxine. On follow-up a patient with malignant lymphoma died, and one patient suffered hypothyroidism. Conclusion HD is a ubiquitous disease. The clinical diagnosis of is still difficult. HD cases complicated with thyroid nodules should be treated surgically to exclude malignant tumors. Intraoperative frozen section is a reliable diagnostic method and may guide the surgical modality.

Purpose: To investigate a clinical treatment for allergic asthma. Method: Acupoint application was used, and its effect was compared with that of Western medicine to evaluate the results. Results and Conclusion: It was found that the long-tern effect of acupoint application was significantly better than that of aminophylline; acupoint application had obviously different effects on different types of asthma based on TCM differentiation of syndromes; its short-term effect was not significantly related to the course and condition of the disease. It is indicated that acupoint application is an effective, convenient and safe treatment with little side effect for asthma.

BACKGROUND: The mRNA expression of α1 chain of type I collagen (COL- Iα1)in rat osteosarcoma (ROS17/2.8) induced by bone morphogenetic protein-2(BMP-2)was reduced under simulated weightlessness. The protein kinase MEK1 in the signal pathway of mitogen-activated protein kinase (MAPK) plays an important role in the expression of COL- I αl mRNA regulated by BMP-2. But there was no report on the kinase activity of MEK1 under simulated weightlessness.OBJECTIVE: To investigate the effects of simulated weightlessness on the activity of MEK1 induced by BMP-2 in ROS17/2.8 cells.DESIGN: A non-randomly control study was conducted.SETTING: Department of Aerospace Biodynamics, Fourth Military Medical University of Chinese PLAMATERIALS: Rat osteosarcoma osteoblast-like cell METHODS: This experiment was conducted at the Laboratory of Aerospace cells and Molecular Biology Laboratory , Institute of Space Medico-Engineering between August 2002 and January 2003. ROS17/2.8 cells were cultured in 1 G control and rotating clinostat simulated weightlessness for 24 hours, 48 hours and 72 hours. The cells were divided into 7 groups as follows: Group 1 was blank control group in which ROS17/2.8 cells were cultured in 1 G condition without BMP2; Group 2, cells were cultured in 1 G for 24 hours;Group 3,in weightlessness for 24 hours; Group 4, in 1 G for 48 hours; Group 5, in weightlessness for 48 hours;Group 6,in 1 G for 72 hours;Group 7,in weightlessness for 72 hours. Cells in Group 2 to Group 7 were all cultured with BMP-2. BMP-2 (500 mg/L) was added into the medium 1 hou before the culture ended. Then the total protein of cells was extracted and the kinase activity of MEK1 was detected by means of Western Blotting.MAIN OUTCOME MEASURES: The content of total ERK1/2 and phosphated-ERK1/2 (p-ERK1/2) protein in the cells RESULTS: ①Total ERK1/2 expression induced by BMP-2 in ROS17/2.8 cells under simulated weightlessness: The total ERK1/2 expression induced by BMP-2 in cells in all the groups showed no significant differences ② Phosphated-ERK1/2 expression induced by BMP-2 in ROS17/2.8 .cells under simulated weightlessness: There was little protein of p-ERK1/2 in ROS17/2.8 cells in Group 1 cultured without BMP-2 in 1 G for 24 hours. The content of p-ERK1/2 in ROS17/2.8 cells in Group 2 cultured with BMP-2 in 1 G for 24 hours was much more than that in Group 1 (P ＜ 0.01). The level of p-ERK1/2 was much lower in simulated weightlessness groups than that in 1 G control groups at the same time point. In other words, the content of p-ERK1/2 in Groups 3, 5 and 7 was respectively lower than that in Groups 2, 4 and 6 (P ＜ 0.01).The expression of p-ERK1/2 showed-a tendency of gradually decreasing in Groups 3, 5 and 7 with the prolongation of time of simulated weightlessness(P ＜ 0.01 ).CONCLUSION: The kinase activity of MEK1 in MAPK signal pathway induced by BMP-2 is reduced under simulated weightlessness.

AIM:To study the chemical constituents of Fissistigma oldhamii(Hemsl.)Merr.METHODS:Silica gel column chromatography was used in the isolation procedure,the structures of the isolated compounds were elucidated by spectral data.Meanwhile cytotoxic activity of compound Ⅰ was made according to the cell experiment in vitro.RESULTS:Five compounds were isolated from Radix of Fissistigma oldhamii(Hemsl.)Merr..On the basis of physical-chemical constants and spectral data(EI-MS,1H-NMR,13C-NMR),these compounds were identified as:Aristolactam A Ⅱ(Ⅰ),Aristolactam B(Ⅱ),Physcion(Ⅲ),?-sitosterol(Ⅳ),Stigmastan-7-one(Ⅴ).Compound Ⅰ showed cytotoxic activities on GLC-82 and HL_ 60 cell strains.CONCLUSION:Compounds Ⅲ,Ⅳ and Ⅴ are firstly isolated from Fissistigma oldhamii(Hemsl.)Merr..The IC_ 50 of compound Ⅰ on GLC-82 and HL_ 60 cell strain are:234.45,101.17 ?M.

Objective: To investigate the ictal epileptic activation using simultaneous electroencephalogram functional MRI(EEG-fMRI),and to study the neural mechanism and the roles of the cerebral cortex and subcortical structures in the generation and propagation of the epileptic activity.Methods: A patient with partial epilepsy was retrospectively found to have slight seizures during the simultaneous EEG-fMRI examination.Two sessions of the ictal functional MRI data were processed and analyzed using the general-linear model.Results: Consistent activation patterns were obtained from the two sessions of data.The positive activation was found over the bilateral parietal lobes,insula cortex,bilateral striatum,thalamus and some nuclei of the brain stem,as well as over the vermis and right cerebellar hemisphere.The maximum intensity point was in the left parietal lobe.Conclusion: The seizure activation is originated from the cerebral cortex,and then propagates through the reciprocal circuits made up of the cortex and the subcortical structures.The cerebral cortex and the brain stem reticular formation are the main structures that propagate the seizure to contralateral hemisphere.The subcortical structures play important roles in the seizure propagation and seizure manifestation.

Objective To study the operative procedure for stage III and IV hilar cholangiocarcinoma. Methods A crescent shape excision on the edges of multiple hepatic segments followed by a 'skirt edge′ form hepatoenterostomy to drain the multiple hepatic ducts was used to treat unresectable stage III,IV hilar cholangiocarcinoma, . Results (1)the mean survival time was 15.65 months;(2)the patient comfortable index was 81.5%;(4)there was no operative death in the series. Conclusions The 'skirt edge' form hepatoenterostomy is a feasible and effective palliative method for unresectable stage III,IV cholangiocarcinoma.

Objective To study the protein components of the secretion of the proximal trunk of transected peripheral nerve and the effect of the secretion on the growth of motor neuron.Methods After the sciatic nerves of rats were transected, the proximal trunk secretion collecting compartment (P-SCC), distal trunk SCC (D-SCC) and (proximal) plus distal trunk SCC (PD-SCC) were set up. The protein components of the solution in the 3 types SCC were analyzed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrum. And the effect of 3 kinds solution on the growth of motor neuron was tested by the motor neuron culture respectively. (Results) All the P-SCC, D-SCC and PD-SCC were filled with light yellow, clear fluids. The protein (concentration) was (5.45?1.2)mg/ml,(3.87?0.7)mg/ml and (5.68?1.5)mg/ml in the solution of PD-SCC, P-SCC and D-SCC (respectively). The protein patterns of the 3 solution were similar, and the detected (protein) spots were 1098?34,985?47 and 1021?36 spots in the solution of P+D-SCC, P-SCC and D-SCC (respectively). Most of the protein spots (distributed) in the rangs of MV 25-90KD and PH 4-8. The results of motor neuron culture demonstrate that the number of survival cells, cell area, neurite length and OD value of experimental groups(including mix group, (proximal) group and distal group) are generally higher than control group. But there is no obvious difference between the three experimental groups. Conclusions (1)The proximal trunk of transected sciatic nerve had the similar secretive ability as the distal nerve trunk.(2)(Protein) image of the 3 kinds of SCC solution showed that proteins from the three SCC solution were homologous. (3)All the 3 kinds of SCC solution could promote the growth and activity of motor neuron, and there was no difference in function between P-SCC and D-SCC solution.

Objective To investigate the relationship between tumor necrosis factor-α (TNF-α) in ischemic brain tissue and bran edema after cerebral ischemia-reperfusion in rats.Methods Eighty four male SD rats were randomly assigned to either a cerebral ischemia reperfusion group (n =44) or a sham-operation group (n =40). A model of middle cerebral artery occlusion for 120 minutes followed by reperfusion was induced in rats using the suture method. The infarct size was determined by 2, 3, 5-triphenyi terazoloride (TTC) staining at 6 h,24 h, 3 d, and 7 d respectively after the reperfusion. Dry-wet weight method was used to measure brain water content and evaluate the extent of brain edema. The enzyme-linked immunosorbent assay (ELISA) was used to detect the concentration of TNF-α in ischemic brain tissue. Results TNF-α level in ischemic brain tissue was increased at 6 h (445.8 ±91.7 pg/ml) after the reperfusion, and reached the peak at day 3 (715.5 ±121.3 pg/ml). There were significant differences compared to the sham-operation group and other time points (all P＜0.001). After that, it was decreased gradually, but it was still higher than that in the shamoperation group at day 7 (478.1 ± 145.5 pg/ml vs. 148.5 ± 101.7 pg/ml, P＜0.005). The initial change of the water content in brain tissue lagged behind the increased TNF-α. It did not increase significantly until 24 h after cerebral ischemia-reperfusion (P ＜0.001). It reached the peak at day 3 (P ＜0.001), and it was still higher than that in the control group at day 7 (P ＜0.05). The evolution of cerebral infarct volume was in accordance with the changes of TNF-α level. Conclusions TNF-α is associated with the changes of brain edema and infarct volume,and it is harmful to brain tissue.

Objective To analyze the adverse drug reactions(ADRs)of edaravone in treatment of patients with acute cerebral infarction(ACI)by reviewing Chinese medical literature and to evaluate safety of edaravone treatment in ACI.Methods Publications from Pubmed and Chinese Biomedical Literature Datababe(CBMdisc)were reviewed and the ADR of edaravone was analyzed among the published 8645 cases.Literatures about randomed-control clinical trails(RCTs)on security of edaravone for treating ACI Was analyzed by meta-analysis.Results Abnormal hepatic function,especially mild elevation of aminotransferase,renal dysfunction and skin rash induced by edaravone were tIle most common ADRs.Among 8645 patients,ADRs were reported in 283(3.27 % ).The meta-analysis in RCTs showed that between the group treated with a combination of edaravone and routine and the group treated with routine treatment only,there was no significant difference in the occurrence rate of ADRs(OR=1.18,95 % CI0.70-2.00,P=0.536),elevation of aminotransferase(OR=1.23,95 % CI 0.57-2.68,P=0.595)or renal dysfunction including albuminutia,increased level of serum ereatinine and nitrogen(OR=1.65.95 % CI0.57- 4.79.P=0.353).Conclusion Edaravone has a low ADRs OCCurrence rate and iS safely used in cerebral infarction treatment.