Objective To evaluate the result of autologous vein graft used as a conduit for repairing the iatrogenic recurrent laryngeal nerve(RLN) deficit.Methods Iatrogenic RLN deficit was repaired by using antogeneous vein graft as a conduit in 17 patients. RLN deficit occurred in right side in 5 cases, in left side in 12. Results The RLN injuries were located within 2 cm below the point of RLN entering throat in 16 cases(94 1%), near the inferior thyroid artery in 1(5 9%). The length of RLN deficit was between 0 5～4 cm. Among the 17 cases, 16(94 1%) were followed up for more than 1 5 years. Of them, normal phonation was achieved in 12 cases(75 0%), significantly improved in 3(18 8%) and slightly improved in 1(6 2%).Among the 15 cases who received indirect or direct laryngoscopy, the affected vocal cord movement entirely recovered in 4 cases(26 7%), partially recovered in 3(20 0%), unchanged in 8(53 3%) Conclusions Autogeneous vein graft is ideal for repairing RLN deficit.

Objective To study the approach to improving the diagnosis and treatment of thyroid cancer(TC). Methods Problems regarding to the diagnosis and treatment of TC had arisen based on the combination of auther's clinical experience and recent literatures review. Results and Conclusions (1) In the diagnosis of TC, attention should be paid to the following conditions: cystocarcinoma, multicentral cancer, bilateral TC, coexist of benign thyroid diseases with TC, and the microcancer of thyroid etc. (2) Biopsy should be done during and after operation for the thyroid nodule needed to be operated, to avoid misdiagnosis of TC. (3) Except undifferentiated carcinoma, the choice of treatment of TC is surgery. The choice of operative procedure should be according to the pathological type and clinical stage of TC, and patient's condition. (4) Thyroid hormone suppression therapy should be administrated after operation. (5) Posterative radiotherapy can promote the long term survival rate. (6) Reoperation should be performed immediately, if the thyroid nodule is TC and comfired by pathological examination posteratively, and a subtotal thyroidectomy or nodule local resection performed on for that nodule initially, to avoid the residual cancer and cancer recurrence in the residual thyroid tissues.

Objective To study the influence of pcDNA3.1-RhoC on the expression of endogenous angiogenic factors in HCC cells.Methods The reconstructed plasmid pcDNA3.1-RhoC was transfected into HepG2 cells, and expression of VEGF and bFGF was detected with the RT-PCR and immunohistochemical stain. HepG2 cells transfected with pcDNA3.1-RhoC or pcDNA3.1 were implanted into nude mice to observe the tumor occurrence rate.Results HepG2 cells transfected with pcDNA3.1/RhoC showed higher expression of RhoC . The expression of RhoC enhanced the expression of VEGF and bFGF(P

Objective To explore the expression of apoptosis regulating genes C jun and bcl X L after normothermic liver ischemic preconditioning and its clinical significance. Methods 16 cases of liver cancer were randomly divided into ischemic reperfusion(IR) group and ischemic preconditioning (IP) group (8 cases in each). The samples of venous blood were drawn before IR or IP procedure and 30 minute after reperfusion for testing ALT, AST and LDH. Meanwhile, samples of liver tissue were taken for study of hepatocellular apoptosis, expressions of C-jun mRNA、 Bcl-X L mRNA and PCNA and morphologic changes. Results The levels of ALT、 AST、 LDH and AI in IR group were significantly higher than those in IP group (P

Objective To investigate Caspase 3 activity in the ischemia reperfusion injury in rats and the protective effect of ischemic preconditioning and its possible mechanism.Methods 30 rats were randomly assigned to ischemia reperfusion (IR) group, ischemic preconditioning(IP) group,sham-operation(S)group.The serum aspartate transaminase (AST),alanine transaminase(ALT),liver caspase 3 activity,and apoptosis index(AI) of hepatocytes were examined in the three groups at 3 hours after repersusion.Results The serum AST,ALT,liver caspase 3 activity and apoptotic hepatocytes were significantly higher in both IP and IR groups than those in S group(P

Objective To investigate the effect of acupuncture at the primary points of the three yin meridians of the foot on blood pressure in an animal model of high/low blood pressure and explore the specific effect of the meridian points-the primary points of the three yin meridians of the foot.Methods The study included the parts of hypertension and hypotenstion. Both parts were allocated to the primary points of the three yin meridians of the foot, non-acupoint, model and blank groups, six rats each. Except the model and blank groups, all the groups were given acupuncture treatment for seven consecutive days. Blood pressure was measured after the completion of treatment. Five consecutive measurements were made in each rat. Mean values of blood pressure were taken.Results For the part of hypertension, blood pressure at each time point was higher in the acupuncture groups than in the blank control group and lower than in the model group, and there were statistically significant differences (P<0.05), indicating that acupuncture at the primary points of the three yin meridians of the foot has a hypotensive effect. The hypotensive effects of Taichong and Taixi were better than those of Taibai and the non-acupoint (P<0.05). The hypotensive effects of Taichong and Taixi were equal (P>0.05). The hypotensive effect of Taibai was equal to that of the non-acupoint (P>0.05). For the part of hypotension, blood pressure at each time point was lower in the acupuncture groups than in the blank control group and higher than in the model group, and there were statistically significant differences (P<0.05), indicating that acupuncture at the primary points of the three yin meridians of the foot has a pressor effect. The pressor effect was worse in the the non-acupoint group than in the primary points of the three yin meridians of the foot group (P<0.05). The pressor effects of Taibai and Taixi were equal but worse than that of Taichong (P<0.05).Conclusions Acupuncture at the primary points of the three yin meridians of the foot has a specific bidirectional regulating effect on blood pressure. Of them, point Taichong produces a best bidirectional regulating effect on blood pressure.

Objective To investigate low molecular weight heparin(LMWH) therapy for pancreatic encephalopathy in severe acute pancreatitis(SAP).Methods SD rats were randomly divided into 3 groups:(1) Sham operation(S) group,(2) SAP group and(3) LMWH treatment(LT) group:LMWH was administrated at 4 hours after construction of SAP model.The levels of serum amylase,myelin basic protein(MBP),tumor necrosis factor alpha(TNF-?),interleukin 6(IL-6),brain water contents and pathological changes of pancreas and brain were measured at 24 h after models were set up in SAP and in S group,and 24 h after LMWH was administrated in LT group.Results(1) The levels of serum amylase,TNF-?,IL-6 in SAP group were significantly higher than those in S group and LT group(all P 0.05).(2) The brain water content in SAP group was significantly higher than that in S group and LT group(P

Objective To investigate the protective efficiency of low molecular weight heparin (LMWH) on lung injury in severe acute pancreatitis (SAP).Methods SD rats were randomly divided into 3 groups:(1) Sham operation (S) group,(2) SAP group,(3) LMWH treatment (LT) group:LMWH was administrated 4 hours after SAP model was constructed.The levels of serum amylase,endothelin1(ET-1),tumor necrosis factor ? (TNF-?),lung wet/dry weight ratios,pulmonary morphology,the expressions of P65mRNA and P65 protein in pulmonary specimens were detected at 1d after the models were set up,and the 3 days survival rate in each group was also observed.Results (1) The levels of serum amylase,TNF-?,ET-1 in SAP group were significantly increased than those in S group and LT group (all P

Objective To explore the relationship between the ultrastructure and gallstone recurrence after cholecystolithotomy.Methods By implanting human cholesterol gallstone into the gallbladder of rabbits, the ultrasructure of the gallbladder was observed with scanning eleetron microscope and transmission electron microscope,3 months before and after cholecystolithotomy.Results ln the stone-implanted group,the epithelium cells were massively damaged,nuclei karyopyknosis,mitochondria destroyed, and phagolysosomes increased. After the gallstone removal, the epitheliune cells did not recover completely where exposed connective tissues and covering surface by mucus. In cytoplasm, the lysosome decreased, and mucus granules secretion increased and actively secreted into the gallbladder lumen.Conclusions The pathologic changes of chronic cholecystitis caused by implanting stones in rabbits is similar to that of the chronic calculous cholecystitis in human.The basic cause of gallstone recurrence after cholecystolithotomy might be that the ultrastructural changes not be restored and the mucus secretion increased obviously in gallbladder.

Objective To explore the treatment effects of low molecular weight heparine (LMWH) on severe acute pancreatitis (SAP). Methods Random analysis were made on the clinical features and therapeutic effect on the control treatment group (CT) in 65 cases and LMWH treatment group (LT) in 77 cases.Results 70(90 9%) cases were cured in LT group,and 48(73 8%) cases were cured in CT group. The incidence of complications in the LT group (28 6%, 22patients 45 times) was significantly lower than that in the CT group (60%,39patients 79 times)(P