Objective To study the influence of electroacupuncture on the expression of Nestin in neural stem cells after focal cerebral ischemia in rats and to explore the mechanism of electroacupuncture in treating ischemic brain injury. Methods The model of middle cerebral artery occlusion was made by using opening cranium hot coagulation in 72 rats, who were divided randomly into a model group and an electroacupuncture group. Electroacupuncture was applied on "Dazhui" and "Baihui" points in rats in electroacupuncture group, and immunohistochemistry method was used to observe the number of Nestin expression positive cells on the 3th, 7th, 14th and 21st days after ischemia. Results There were Nestin positive cells in the cortex, hippocampal dentate gyrus (DG) and striatum at different time points after cerebral ischemia, and the number of Nestin positive cells in various brain regions were increased significantly on the 7th day (P

Autogenous nerve transplantation is the best means for treating peripheral nerve injury. However, it would detriments function of donor area. Therefore, homologous nerve transplantation is adopted in clinic. The key of successful nerve allograft transplantation is to reduce nerve allograft antigenicity. Compared to physics method, chemistry method achieved a better result in reducing antigenicity of allograft nerve. Here, we introduce immune response and characteristics of grafts following homologous nerve transplantation, discuss the pretreatment method which can reduce the antigenicity. And propose that the combination of one or many kinds of methods would decrease the antigenicity of homogenous nerve in future.

Objective To investigate the expression of nuclear factor-?B(NF-?B) around the hematoma by the intervension of APS in rat, to study mechanisms of APS protecting brain and its effect on inflammation after intracerebral hemorrhage. Methods ICH models was induced in rats by injection ofⅦtype collagenase into the right globas pallidus. APS was used as the intervension drug. Immunohistochemisty was used to investigate the dynamic changes of the expression of NF-?B in the tissue surrounding hematoma in ICH rats. Ultrastructures of the cells around the hematoma were observed with transmission electron microscope. Results Compared with model group, the scores of neurological behavior in treatment group rats were decreased significantly at 3 day and 5 day (P

With the burgeoning of continuing medical education, grass-roots hospitals are confronted with quite a number of problems in the implementation of the task, such as a general rush into action with each department going its own way, incontrollable educational expenses that are beyond the hospitals, difficulties in developing programs at the hospital level because of the limitation of academic disciplines, and the buying and selling of credits. In view of the above problems, the authors put forward some suggestions for formulating a series of continuing medical education management strategies that both conform to the situation in our country and suit the needs of health professionals at grass-roots institutions.

0.05).(3) IL-17 was positively correlated to CRP(r=0.460,P 0.05).Conclusion:(1) IL-17 in patients is much higher than that in normal people,and is positively correlated to CRP,the acknowledged marker of inflammation status,but not correlated to dialysis duration,suggesting that IL-17 plays a role in pathogenesis of immune anomaly and micro-inflammation in patients with ESRD and HD;(2) There is no correlation between IL-6 and IL-17,and the mechanism of IL-17 elevation in ESRD patients needs to be further investigated.

Objective To analysis the DNA sequence of HEV ORF3 cDNA and express in HepG2 cell .Methods Recombinant vector carrying HEV ORF3 cDNA was constructed and identified by DNA sequencing .Expression of recombinant vector in HepG2 cell was determined by immunofluorescence technique .Results The recombinant vector was constructed and expressed in HepG2 cell successfully .Conclusion The research laid experimental foundation for exploring the biological function of ORF3 protein in the pathogenesis of viral hepatitis E .

Objective To explore the relationship between gene mutation of JAK2 V617F, JAK2 (exon12), CALR, MPL and clinical features of patients with bcr-abl negative myeloproliferative neoplasms (MPN), and to analyze the risk factors of thrombosis. Methods Clinical features and laboratory tests of 115 patients with bcr-abl negative MPN were analyzed retrospectively. 34 patients with thrombosis were treated as the observation group, and 81 patients without thrombosis were treated as the control group. Results Among 71 primary thrombocythemia cases, the white blood cell count (WBC) and hemoglobin level of JAK2 V617F positive group and CALR positive group was higher than that of 4 gene mutations in negative group (F= 5.835, P= 0.005; F= 3.405, P= 0.039). The incidence of splenomegaly in JAK2 V617F positive group and CALR positive group was higher than that of 4 gene mutations in negative group (χ2=16.902, P=0.0002; χ2= 12.658, P= 0.001). The patients'proportion of JAK2 V617F positive, high hemoglobin level (male ≥160 g/L, female ≥150 g/L), hypertension and over 60 years old in the observation group was higher than that in the control group (χ2= 5.585, P= 0.025; χ2= 4.909, P= 0.043; χ2= 8.891, P= 0.004; χ2=15.933, P=0.023). Conclusion The detection of JAK2 V617F, JAK2 (exon12), CALR and MPL gene mutations is helpful to the diagnosis of bcr-abl negative MPN; JAK2 V617F positive, high hemoglobin level, hypertension, and elderly age are risk factors of thrombosis.

In this paper, a newly-designed device is introduced, which uses non-dispersive infrared absorption technique to acquire anesthesia gas concentration signal and to perform side-stream measurement in the air way. The microcontroller is adopted to implement data acquisition, data processing and then to transmit the data to the PC screen, thus the real-time continuous invasive concentration monitoring of the anesthesia gas in the patient's respiratory air can be realized during a surgical operation.

Objective: To study the e ects of Heying Anxin Fang on cardiac function and intracellular Ca2+ concentration in cadiocyte of chronic congestive heart failure rats. Methods: Animal models of heart failure were induced by subcutaneous injection of a large dose of ISO, and were randomly divided into model group, Shenmai injection group, captopril group, high, middle and low dosages of Heyinganxin Fang group and normal group with 9 in each group. To determine the indexes of LVSP, LVEDP, +dp/dtmax ,-dp/dtmax and heart weight, and determine Ca2+ concentration in cardiomyocytes by ow cytometer. Results: Compared with normal group, the indexes of heart weight, Ca2+ concentration and LVEDP in model group increased, but indexes of LVSP, +dp/dtmax, -dp/dtmax decreased obviously(P

Objective To investigate the effects and mechanism of magnetic microsphere combination of cisplatin with nano or micron ferric oxide particle on human hepatoma cell(HHC). Methods A human hepatoma cell line BEL-7402 was used in this study, 0.04?g/ml～8?g/ml of cisplatin(CDDP), cisplatin nano-ferric oxide magnetic microsphere(nCDDPmm) and micro-ferric oxide magnetic microsphere(mCDDPmm) were administered to the culture solution for culturing the BEL-7402 cell as the experimental groups,and in the control groups were administered with cisplatin 0?g/ml and magnetic microsphere without cisplatin respectively. The optic density of viable cell, cytotoxity index, growth curve of cell, cell cycle, proliferation index and apoptosis were assayed by MTT method, cell count and flow cytometry respectively. Results (1)The viable cell's optic density decreased and the cytotoxity index increased in human hepatoma cell following increasing dosages of CDDP, nCDDPmm and mCDDPmm in culture solution,presenting a dose-dependent manner(r value of three drugs were 0.95, 0.91 and 0.89 respective, P 0.05)between nCDDPmm and mCDDPmm. (3)Apoptosis rate of hepatoma cell increased ( P