Objective:To explore the significance of homocysteine, high sensitivity C reactive protein (CRP) and Color Doppler ultrasound in ischemic stroke prognosis.Methods: Two hundred cases of ischemic stroke patients received in neurology department of our hospital from Jan 2012 to Jan 2015 were divided into non-progressive stroke group (110 cases of patients) and progressive stroke group (90 cases of patients) according to the clinical manifestations and diagnosis results of 200 patients. All common carotid artery intima-media thickness of all patients were measured by Color Doppler ultrasound. General information and carotid artery intima-media thickness in the two groups of patients were compared; homocysteine level and high-sensitivity C protein level in the two groups of patients on admission and after two weeks of treatment were compared.Results:Differences of general information between the two groups were not statistically significant (P>0.05); average carotid artery intima-media total thickness in progressive stroke group is (1.54±0.39)mm, significantly higher than non-progressive stroke group[(1.08±0.23) mm], the difference was statistically significant (t=10.37,P<0.05); Hcy levels in progressive stroke group on admitted to hospital and after two weeks’ treatment were significantly higher than non-progressive stroke group, the differences were statistically significant (t=3.343, 5.104,P<0.05); Hs-CRP levels in progressive stroke group on admitted day and after two weeks’ treatment were significantly higher than non-progressive stroke group, the differences were statistically significant (t=7.062, 7.552,P<0.05).Conclusion:Serum homocysteine levels and high-sensitivity C-reactive protein levels can be used as important indicators for prognostic evaluation of ischemic stroke, combining with Color Doppler ultrasound diagnosis can get more precise effect, and worthy of promotion.

Objective:To discuss the application of Roche ECL instrument E601 in inspection serum markers of myocardial injury aspects.Methods: Dependent on the clinical experience of cardiology, the introduction of Roche E601 methodology and the instruments using, and the clinical significance analysis of myocardial injury markers, summarized the important considerations in accurate test results and clinical judgment when using ECL instrument checks serum markers of myocardial injury.Results: In order to make better clinical application, people who use Roche ECL instrument E601 should pay attention to proper storage and use reagents, note the use of the combined reagents and the clinically meaningful of detected projects. Conclusion: The quality control of technical procedures is very important to assure the testing results. Only understanding some of the important considerations,can we better integrate the device for clinical application.

Objective: To analyze the clinical value of 16-slice CT angiography techniques in cerebral artery lesions. Methods:Forty three cases in our hospital with suspected cerebrovascular disease who performed computed tomography angiography were retrospectively analyzed. Volumetric imaging, maximum intensity projection and multi-planar reconstruction technique for three-dimensional reconstruction process were used in post-processing. Results:There were 27 cases with no abnormal blood vessels, 6 cases with aneurysm, 1 with arteriovenous malformation, 8 with arterial stenosis and 1 with congenital abnormalities. The image quality analysis of cerebral vascular trunk and main branches was 100%performed in 43 cases. The location, shape, size, and the relationship with the surrounding blood vessels and skull of the aneurysm have been clearly shown, and the nidus, feeding arteries and draining veins have also been clearly shown. Conclusion:With the features of safe, fast, non-invasive and clarity in images, 16-slice CT angiography imaging technology can be used as the first choice of screening cerebral arterial disease.

ObjectiveTo characterize the chemical constituents of Dayuanyin based on ultra-performance liquid chromatography-quadrupole/electrostatic field orbitrap mass spectrometry（UPLC-Q-Exactive Orbitrap MS）. MethodThe detection was performed on a Thermo Acclaim™ RSLC 120 C₁₈ column（2.1 mm×100 mm， 2.2 μm）， the mobile phase was acetonitrile（A）-0.1% formic acid aqueous solution（B） for gradient elution （0-7.5 min， 10%-19%A； 7.5-12 min， 19%-22.5%A； 12-23 min， 22.5%-27%A； 23-27 min， 27%-56%A； 27-35 min， 56%-84%A； 35-36 min， 84%-90%A）， the flow rate was 0.3 mL·min^-1， and the column temperature was 30 ℃. The data were collected in the positive and negative ion modes by heated electrospray ionization（HESI）， and the detection range was m/z 80-1 200. Combining the retention time of the reference substance， fragment ions， databases such as PubChem and related literature， Xcalibur 3.0 was used to identify the chemical constituents of Dayuanyin. ResultA total of 161 compounds were identified， including 14 alkaloids， 60 flavonoids， 16 terpenoids， 26 saponins， 18 phenylpropanoids， 16 organic acids and 11 others. ConclusionThe established method can effectively and quickly identify the chemical components in Dayuanyin， and clarify its chemical composition， which can provide a basis for the development of compound preparations of this famous classical formula.