AIM: To observe the efficacy of hemoperfusion (HP) to treat one case suffering from overdose digoxin poisoning. METHODS: HA 330 ml and YT 160 hemoperfusion cartridges were used and blood vessel was femoral vein, single needle and two chamber catheter. Four HP were done. RESULTS: The blood digoxin level were 16 ?g?L -1 before and after the 1st HP, 9.22 ?g?L -1 at the 2nd, 12.4 ?g?L -1 before the 3th, 10.45 ?g?L -1 after the 3th, 3.22 ?g?L -1 before the 4th, and 2.84 ?g?L -1 after the 4th HP. CONCLUSION: This case demonstrats that multiple HP at different period may decrease the blood digoxin level step by step.

Objective To evaluate the efficacy of trans-ulner or trans-radial approach to coronary revaseularization with titanium-nitride-oxide-eoated stent (TITAN2). Methods 31 patients,who were planed to receive coronary revaseularization,were selected into this study. All patients with coronary revascularization were either by trans-ulner or by trans-radial approach. The pass-through rate of stent, early thrombosis in stent and MACE at followup were recorded for TITAN2. Results 46 lesions with stenosis > 75% with coronary angiograpby in 31 patients successfully underwent coronary revaseularization with TITAN2. One stent didn't go through the lesion in RCA. The pass-through rate of stent was 97.8%. Remaining stenesis ,stent unglues,endarterium laceration ,early thrombosis in stent and coronary rupture were not discovered after stent deployment. MACE was 0 at 1-5 month follow-up. Conclusion Confirmed good efficacy in coronary revascularization with TITAN2 is observed.

Objective To establish the baseline data of body weight, main organ weights, hematological and biochemical indexes in SPF congenital cataract mice. Methods Body weight, main organs weights, hematological and biochemical indexes of the congenital cataract mice were determined at 28 days and 56 days of age, respectively. Normal KM mice in the same age were taken as control. Results There were no statistically significant differences in all indexes of the mice at 28 days of age. Compared with the 56?day old normal KM mice: (1) Statistically significant differences were found in the body weight, and weights of the heart, liver, spleen, lung, kidney and testis ( P<0?05 or P<0?01; ( 2 ) Statistically significant differences were found in hematological indexes WBC, PLT, MPV, LYMP, PDW for female mice and MPV, PDW for male mice (P<0?01);(3) Among the biochemical indexes, there were also statistically significant differences in UREA, ALP, TP, UA, TG, GLU for female and ALT, ALP, TP, ALB, UA, GLU for male mice. Conclusions There are statistical differences in the body weight, main organ weights, hematological indexes and biochemical indexes between the congenital cataract mice and normal KM mice at 56 days of age. These results may provide a useful reference for future research.

The clinical data of 239 inpatients with type 2 diabetes in Endocrinology Department of Henan Provincial People′s Hospital from January to February 2017 were collected. A total of 170 subjects were included in the final analysis. One diabetes-specific vertebral facture risk estimation called risk score for vertebral fracture was used to estimate the risk of vertebral fracture. Multivariate linear regression analysis was used to calculate the association of serum uric acid with risk of vertebral fracture. The mean age of subjects in final analysis was 56.5±26.4 years old, with the duration of diabetes 8.6±7.1 years and the vertebral fracture risk score 5.6±4.0. Additionally, there was a negative linear correlation between serum uric acid and vertebral fracture risk score in patients with type 2 diabetes( Ptrend=0.021) independent of age, gender, systolic blood pressure, HbA 1C, course of diabetes, obesity status, total cholesterol, and estimated glomerular filtration rate( P=0.033). Multivariate linear regression indicated that age, course of diabetes, blood pressure, total cholesterol, serum albumin, T score at femoral neck were related to the vertebral fracture risk score.

Objective To prepare a human meningococcal reference serum and standardize IgG concentrations to capsular polysaccharides and in vitro bactericidal activities of the reference serum against serogroup A, C, Y and W135 strains.Methods Twenty healthy adults were recruited and given one dose of immunization with tetravalent (serogroups A, C, Y and W135) meningococcal polysaccharide vaccine . Plasma samples were collected and gone through a series of process treatments including defibrination , filtra-tion, and lyophilization to prepare the meningococcal reference serum Men 10.The IgG concentrations of Men10 to capsular polysaccharides of serogroups A , C, Y and W135 were calibrated by using an internation-al reference serum CDC1992 as the standard in enzyme-linked immunosorbent assay (ELISA).Provisional IgG concentrations of Men10 were intensively validated by testing a panel of 12 calibration serum samples from Centers for Disease Control and Prevention , USA ( US CDC) and a panel of 56 serum samples immu-nized with A, C, Y and W135 meningococcal polysaccharide vaccine from Lanzhou Institute of Biological Products Co., Ltd.(LIBP) with the assays using Men10 and CDC1992 as the standard and/or test sam-ples, respectively.The bactericidal titers against serogroup A , C, Y and W135 strains were measured by se-rum bactericidal assay (SBA).Results Four thousand vials (0.5 ml/vial)of lyophilized human meningo-coccal reference serum Men10 were successfully prepared with 2.5%of residual moisture .Reference serum Men10 was sterile and free from contamination by hepatitis B virus , hepatitis C virus , human immunodefi-ciency virus and syphilis .Provisional IgG concentration of Men 10 to capsular polysaccharide of serogroups A, C, Y and W135 was calibrated by using CDC1992 as the standard.Furthermore, IgG concentrations of both panels of 12 CDC calibration serum samples and 56 LIBP serum samples calibrated by using Men 10 as the standard correlated well with those by using CDC1992 as the standard (r=0.99,P<0.05).The IgG concentrations of CDC1992 as calibrated by using Men10 as the standard showed significant correlation with its previously determined values with variation <10%.SBA titers for serotype A , C, Y and W135 strains were established as well .Conclusion A panel of new human meningococcal reference serum Men 10 with accurately calibrated IgG concentration against capsular polysaccharide of serogroups A , C, Y and W135 as well as SBA titers was successfully established .

Objective:To investigate the correlation between serum calcium levels and severity of novel coronavirus pneumonia(COVID-19).Methods:The clinical data of 165 COVID-19 patients diagnosed from January to February 2020 were analyzed retrospectively. Combined with clinical classification, the differences of various indexes between the critically ill group and the control group were compared, and the influencing factors of disease severity were analyzed by multivariate logistic regression. According to the corrected serum total calcium levels, patients were divided into low calcium group and normal calcium group, and the related indexes of the 2 groups were compared for further analyzing the causes of hypocalcemia. Results:Compared with the control group, the age, diabetes, basic respiratory disease, and cardiovascular disease ratio, C-reactive protein(CRP), fasting blood glucose(FPG), interferon γ(IFN-γ), and interleukin 17(IL-17) levels increased while the lymphocyte percentage, serum albumin(ALB), corrected calcium levels, CD4 + T cells percentage, CD8 + T cell percentage decreased, the difference was statistically significant( P<0.05). There was no significant statistical difference in gender between the two groups, hypertension ratio, alanine aminotransferase(ALT), glomerular filtration rate(eGFR), CD4 +/CD8 + ratio and interleukin 4(IL-4) levels( P>0.05). The decrease of calcium level, age and eGFR were all risk factors for COVID-19 patients. Compared with the normal calcium group of COVID-19 patients, the level of ALB, CD4 + T cells percentage, CD8 + T cell percentage in low calcium group decreased and age, proportion of critically ill patients, diabetes, basic respiratory disease and cardiovascular disease ratio and CRP level all increased, the differences were statistically significant( P<0.05), and there was no statistical difference in the other biochemical indexes( P<0.05). Conclusion:There are obvious hypocalcemia and immune dysfunction in critically ill patients of COVID-19, and close monitoring of blood calcium levels may predict the severity of the disease more effectively

Objective:To investigate the association between time in target range and risk of vertebral fracture in patients with type 2 diabetes.Methods:The clinical data of 1 032 patients with type 2 diabetes who were hospitalized in endocrine department of Henan Provincial People′s Hospital from June 2017 to July 2021 were collected. Among which 632 patients were included into final analysis. The diabetes-specific risk score for vertebral fracture was used to assess the risk of vertebral fracture. Multivariate linear regression analysis was used to test the association between time in target range and risk score of vertebral fracture. Risk score≥9 was defined as increased risk of vertebral fracture. Multivariate logistic regression was used to estimate the association between time in target range and risk of vertebral fracture. Results:Among the included patients, mean age was(55.0±12.4) years and the percent of male was 72.5%. The mean course of diabetes was(9.4±8.0) years, and mean score of risk of vertebral fracture was 5.6±4.3. Time in target range was negatively correlated with risk score of vertebral fracture( P for trend <0.001), which was independent of potential confounders and continuous glucose monitoring parameters. The included patients were divided into four groups based on quartiles of time in target range. Multivariate logistic regression indicated that the risk of vertebral fracture in the first quartile of time in target range was 4.6 times higherthanthatinthe4thquartile, and the significance remained adjusted for potential confounders, s, CV, or meanamplitudeofglycemicexcursions(MAGE), respectively. Conclusion:Timein target rangewasnegativelycorrelatedwithriskscoreofvertebralfracturein patient with type 2 diabetes. Low time in range level was an independent risk factor for increased risk of vertebral fracture.

Objective:To analyze the correlation between two-dimensional ultrasound and HbA1c, Thyroid stimulating hormone (TSH) and its diagnostic value in benign and malignant Thyroid nodules.Methods:A total of 155 patients with thyroid nodules examined in the Department of Ultrasound Medicine of Haikou Orthopedics and Diabetes Hospital from January 2020 to January 2021 were prospectively selected. With surgical pathological examination as the gold standard, they were divided into benign group (75 cases) and malignant group (80 cases) according to the nature of nodules. There were 40 males and 35 females in the benign group, with an age of (47.95±8.26) years old and BMI of (22.45±1.26) kg/m2. There were 42 males and 38 females in the malignant group, with an age of (48.22±8.01) years old and BMI of (22.36±1.21) kg/m2. The expression level of HbA1c was detected by automatic biochemical analyzer, and the concentration of TSH was detected by automatic immunoassay. SPSS 19.0 statistical software was used for analysis and processing.Results:Compared with benign thyroid nodules, the mean transit time (MTT) and time to peak intensity of malignant thyroid nodules were (0.92±0.11 vs 1.48±0.42) s and (1.48±0.42) s respectively. TIP (1.06±0.21 vs 1.57±0.56) s, perfusion Index (PI) (1.15±0.22 vs 1.76±0.62) s parameters increased, and the differences were statistically significant ( P < 0.05) . Compared with patients with benign thyroid nodules, the expression levels of HbA1c (6.98±0.73 VS 10.22±1.35) % and TSH (3.95±0.53 VS 8.75±0.95) mIU/L in patients with malignant thyroid nodules were significantly higher ( P<0.05) . The expression levels of MTT, TIP, PI, HbA1c, and TSH were correlated with those of benign and malignant thyroid nodules, and the expression levels of MTT, TIP, PI, HbA1c, and TSH were positively correlated with those of benign and malignant patients ( P<0.05) . MTT, TIP and PI had correlation with the expression levels of HbA1c and TSH. Positive correlation: MTT had a positive correlation with HbA1c and TSH. TIP had a positive correlation with HbA1c and TSH. PI had a positive correlation with HbA1c and TSH ( P<0.05) . Compared with the single diagnosis of MTT, TIP and PI, the three combinations had higher diagnostic value for malignant thyroid nodules ( P=0.001) . Conclusion:Two-dimensional ultrasound parameters have high diagnostic value in malignant thyroid nodules, and have certain correlation with HbA1c and TSH, which can be used for the diagnosis of malignant thyroid nodules.

Objective To examine the diagnosis and outcomes in the treatment of the patients with histologically confirmed central neurocytoma (CNC). Methods The data from 71 patients with CNC who were diagnosed between March 2003 and December 2007 were retrospectively evaluated. Various combinations of surgery, and radiotherapy had been used for treatment. Results The average bulk of tumors was 40 cm3. The median follow-up was 22 months. The 22 months overall survival and local control rate was 95.8%(68/71) and 95.6%(65/68), respectively. Conclusions The overall prognosis is favorable although the follow-up is not very long. Surgery and postoperative radiotherapy can significantly improve local control.

Objective To analyze and estimate, the treatment of patients with histologically confirmed subependymal giant-cell astroeytoma (SEGCA). Methods The data from 23 patients with SEG-CA who were diagnosed between February 1995 and February 2008 were retrospectively evaluated. Various combinations of surgery and radiotherapy had been used for treatment. Results Total resection was 16 cases, subtotal resection was 7 cases, radiotherapy was 17 cases. The average follow-up time was 53 months.One postoperative SEGCA recurrence. Epilepsy was totally disappeared in 17.6% (3/17), partly disappeared in 47.1%(8/17). All cases survived. Conclusions The key of treatment is total resection. The significance of radiotherapy is not sure. The overall prognosis of SEGCA is favorable.