Objective:To investigate the environmental factors a nd individual personalities of anorectic children in order to improve their nu rsing care.Methods:Forty-six children with anorexia caused by emotional disorders and 50 healthy children were tested by a societa l-psychological factor questionnaire and the Eysenck Personality Questionnaire (Junior) to observe their external environmental factors and individual persona lities,respectively.Results:Encidence of pressures from school work,forced eatin g by parents and family discord emerged more frequently in the anorectic group than in the co ntrol group.Scores in the anorectic group were significantly lower in the scale E level and markedly higher in the scale N level than those of the control group (P<0.01).There was no significant difference between the two groups in the P and L scales .Conclusion:The three factors mentioned above were most closely related to onset of disease, among the factors we observed.Both an intro verted personality and unstable emotions are key,characteristics that can induc e anorexia when affected by these factors.Comprehensive nursing care,mainly cons isting of psychiatric-behavioral correction,is proposed to obviate the inducing factors and rectify emotional disorders to promote recovery from anorexia.

Objective To investigate the neuroprotection of different brain regions after transplantation of genetically modified myoblasts producing brain derived neurotrophic factor (BDNF) into cortices of neonatal rats subjected to hypoxic ischemic encephalopathy (HIE). Methods Seven day old pups were randomized into sham operated group (C), HIE+BDNF group (B) and HIE+mock transplantation group (A). A rat myoblast cell line expressing and secreting BDNF (BDNF(+)/L 6TG) was constructed. A stereotaxical intracerebroparenchymal transplantation of either BDNF(+)/L 6TG (B) or BDNF(-)/L 6TG (absence of BDNF, A) at 0.8 ?l of cell suspension (4?10 4/?l) into the left cortex of the brain was carried out shortly after HIE undergone by ligation of left common carotid artery followed by a 2.5 h inhalation of humidified 8% O 2+92% N 2 at 37℃. Changes of areas of different brain regions of cortex, hippocampus and striatum at different sections were observed 21 d after the procedure. Injury severity scores of brain tissues were also performed. Results Twenty one days after the manipulation, various degrees of atrophy were observed in the three regions of left hemispheres in group A or B versus contralateral corresponding regions or group C whereas this atrophy in group B was significantly reduced when compared to group A. Not only was the atrophy of cortex near the grafting point alleviated but some other regions of left hemispheres. A similar change pattern was seen in injury severity scores of the tissue. Conclusion Present data suggest that intracerebral transplantation of genetically modified myoblasts producing BDNF has a beneficial effect on the protection from damage of both circumscriptus brain tissues and some other areas distanced from the grafted place.

High incidence of postoperative abdominal adhesions after gynecological laparoscopy, which may induce a line of serious complications such as chronic pelvic soreness, intestinal obstruction, infertility, etc, has been clinically encountered with the major problems unsolved thus far. By further upgrading the merits of gynecological laparoscopy, including the small operative scope, mild injury and fine manipulation, coupled with the application of anti-adhesion therapies, reduced post-operative adhesions and improved clinical outcomes can be expected. Research has been actively persisted on this area in recent years to achieve new progress, especially in the development of anti-adhesion materials, as well as their clinical application and evaluation of the effects, which is overviewed in this review.

AIM To observe the effects of different interval administration of exogenous brain derived neurotrophic factor (BDNF) on hypoxic ischemic encephalopathy in neonatal rats. METHODS BDNF (0 5 ?g) was microinjected intracerebroventricularly at 0, 1 and 4 h after the hypoxic ischemic encephal opathy in 7 d neonatal Sprague Dawley rats undergone by ligation of left common carotid artery followed by a 2 5 h inhalation of humidified 8% O 2+92% N 2 at 37℃ immediately after the injury, respectively. Changes of brain edema, levels of malondialehyde (MDA) and neuronal apoptosis at the left cortex and hippocampus were investigated 24 h after the injury. RESULTS The most prominent effect of BDNF was seen in 0 h group with a marked decreases in brain edema and levels of MDA and a significantly alleviated neuronal apoptosis while it was lowered obviously when administered at 4 h after the insult. CONCLUSION BDNF exerts a prominent protective effect on hypoxic ischemic encephalopathy in neonatal rats when given as early as possible after the injury rather than later administration.

Objective To study the relationship between velocities of ductus venosus(DV),inferior vena cava(IVC) and right hepatic vein(RHV) of fetus,and abnormal perinatal outcome in high-risk pregnancy so as to find out which venous velocity can predict abnormal perinatal outcome and which velocity is the best for predicting abnormal perinatal outcome. Methods Velocities of DV,IVC and RHV in 67 cases of high-risk pregnancy were examined through color Doppler and their relationship with perinatal outcome was analyzed respectively. Results RHV-pulsatility index(PI) ,DV-pre-load index(PLl) ,DV-S/A and IVC-peak velocity index(PVI) could all predict abnormal perinatal outcome in high-risk pregnancy. But RHV-PI had the best prediction, and when its positive cutoff value was taken as 2. 89,the sensitivity for abnormal outcome was 75. 0% ,the specificity was 76. 7% ,and the correct index (Youden index) was 0. 52. Conclusions RHV-PI can best predict abnormal perinatal outcome in high-risk pregnancy,which can help a lot for prompt clinical treatment.

To explore the effects of brain derived neurotrophic factor (BDNF) on hypoxic ischemic encephalopathy in neonatal rats,0 2, 0 5 or 2 0?g of BDNF was injected into cerebroventricles of 7d neonatal Sprague Dawley rats with hypoxic ischemic encephalopathy by ligation of left common carotid artery and 2 5h inhalation of humidified 8% O 2 +92% N 2 at 37℃. Its effects on brain edema, levels of malondialehyde (MDA) and neuronal apoptosis at the left cortex and hippocampus were observed. The results showed that brain edema was markedly alleviated in 0 5?g group compared to the vehicle group 24h after BDNF injection. Levels of MDA in the left hemisphere in 2 0?g group were significantly higher than those in either vehicle group or 0 5?g group. Neuronal apoptosis decreased to a different extent in the three groups while obviously in both 0 2?g and 0 5?g groups. It is suggested that BDNF exerts a prominent protective effect on hypoxic ischemic encephalopathy in neonatal rats, but this effect is lowered when overdose is injected.

Objective To analyze the constituent and clinical features of the senile females who underwent surgery for gynecological tumors and traumatic diseases,so as to enhance the surgical safety and efficacy.Methods From Jan.2003 to Dec.2006,a total of 178 female patients,who were undergone surgery for gynecological tumors or traumatic diseases,were involved in present study,of whom the clinical data,the patients' constituent and pre-operative complications were retrospectively analyzed.The cases selected were allocated into two groups according to their age:the observation group included 116 patients with age ≥60,among them 86 cases were undergone operation for gynecological tumors and 30 cases for traumatic diseases;the control group included 62 patients with age of 40-50 and comparable parity and body weight,among them 54 cases were undergone operation for gynecological tumors and 8 cases for traumatic diseases.Results The number of the patients operated for gynecologic tumors remained the majority annually,whereas a yearly increase of traumatic diseases was noticed with 3.1 and 1.8 folds increasing in 2006 compared to that in 2003 and 2004,respectively(P

Objective:To study the changes of plasma orphanin (OFQ) level in post-partum depression (PPD). Methods:The level of plasma OFQ of 24 patients with post-partum depression (PPD group) and 25 healthy lying-in women (control group) were measured by radioimmunoassay.Results:The level of plasma OFQ in PPD group was 27.39?6.04 ng/L , and that of control group was 10.37?3.65 ng/L,the difference was statistically significant(P

Objective To explore the effects of brain derived neurotrophic factors (BDNF) on concentrations of cerebral endogenous opioid peptides(EOP)in neonatal rats subject to with hypoxic ischemic brain injury (HIBI). Methods Seven day old Sprague Dawley rats were randomly divided into a HIBI+BDNF group (group A),a HIBI group (group B) and a sham operation group (group C). Models of HIBI were established by use of permanent ligation of the left common carotid artery followed by 2.5 hours ofinhalation of 8%O 2+92%N 2, then 0.5 ?g BDNF was injected into the parietal cerebral ventricle in group A immediately. Contents of dynorphin A 1 13 like, ? endorphin like and leu enkaphalin like immunoreactivities (ir DynA 1 13 , ir ? EP and ir LEK) in cortex and hippocampus were measured at 0, 60, 120 min after administration of BDNF. Results The concentrations of ir DynA 1 13 and ir ? EP in the cortex and hippocampus in group B were increased significantly than those in group C at most time points( P

BACKGROUND: Endogenous opioid peptide is an important medium and regulator that participate in many physical and pathologic processes of the body. Its relationship with fetal encephalopathy has attracted much attraction.OBJECTIVE: To evaluate the role of endogenous opioid peptides (EOP) in fetal distress.DESIGN: A case-control observatory study based on healthy pregnant women.SETTING: Wards of the Department of Obstetrics and Gynecology of Changzheng Hospital Affiliated to Second Military Medical University of Chinese PLA.PARTICIPANTS: Eighty-three healthy women who were hospitalised in Changzheng Hospital and Changhai Hospital affiliated to the Second Military Medical University of Chinese PLA and met the inclusion criteria. Among them, 40 were normal healthy pregnant women(the control group) and 43 were healthy pregnant women with fetal distress(the fetal distress group).METHODS: Radioimmunoassay was used to measure the levels of blood EOP(β-endorphin, dynorphin A1- 13 and leu-enkephalin) of the venous blood of the pregnant women in fetal distress group and the control group and the EOP level in the umbilical blood of the newborns. Also, blood gas analysis of the blood from the umbilical artery was conducted.MAIN OUTCOME MEASURES: The levels of EOP in the venous blood of two groups of pregnant women and the umbilical blood of newborns and the correlation of EOP level with fetal distress.RESULTS: The levels of the umbilical artery blood EOP(β-endorphin,dynorphin A1-13 and leu-enkephalin) in the fetal distress group[(453± 68 ) ng/L, (242 ± 33)ng/L, and(498 ± 68)ng/L respectively] were significantly higher than those in the control group[ (251 ± 39) ng/L, (103± 22 )ng / L and(322 ± 40 )ng / L respectively ( t = 2. 713,2. 762, P＜ 0.01; t = 2. 132, P ＜ 0.05 ) ]. The umbilical artery blood gas analysis;pH was (7.0 ± 0. 1 ) , PO2 was ( 1.7 ± 0.6) kPa, PCO2 was (8.9 ± 0. 7) kPa.The levels of β-endorphin were negatively correlated with pH and PO2 of the umbilical artery blood(r= -0.418 and -0.437, P ＜ 0.01), but they were positively correlated with PCO2( r = 0. 442, P ＜ 0. 05) . The level of dynorphin A1-13 was negatively correlated with pH and PO2( r = -0. 337,-0.383, P ＜ 0.05), but it was positively correlated with PCO2(r= 0. 346, P ＜ 0. 05). There was no significant difference among the three kinds of blood EOP of the two groups( P ＞ 0.05).CONCLUSION: EOP participates in the pathological progress of the fetal distress and was closely correlated with the occurrence and development of the fetal distress. This finding has a reference value for early rehabilitation and intervention after the fetal was born that can be tested quantitatively.