Objective To compare the effects of early and conventional instillation of normuscle invasive bladder cancer after transurethral resection of bladder tumor.Methods The clinical data of 32 patients with normuscle invasive bladder cancer were retrospectively analyzed.They were divided into observation group (16 cases) and control group (16 cases).The clinical effects of the two groups were compared.Results The recurrence rate of two groups was significantly different (x2 =3.864,P ＜ 0.05).The average recurrence time of two groups was significantly different (t =6.389,P ＜ 0.05).The incidence rate of adverse reactions (x2 =0.369,P ＞ 0.05) and the incidence rate of toxic reactions(x2 =2.222,P ＞ 0.05) in two groups had no statistically significant differences.Conclusion It can lower the recurrence rate for early and conventional instillation of normuscle invasive bladder cancer after transurethral resection of bladder tumor and to delay the recurrence time,so it is widely applicable for clinic cure.

Tumor may generate from tumor stem cell and the microenvironment,and cancer stem cells are derived from the mutation of normal stem cells according to cancer stem cells hypothesis. Breast cancer stem cells are the first identified cancer stem cells in solid tumor. Breast cancer stem cells have been isolated successfully by many kinds of strategies, and their biological behaviors are gradually studied deeply. Self-renewal and differentiation of breast cancer stem cells are regulated by microenviroment and many signaling pathways. Therapy targeting breast cancer stem cells is gradually becoming the focus of tumor targeted therapy study.

Objective:To investigate the problems and countermeasures primary hospital radiology digital construction.Methods: The analysis of the current characteristics of the primary hospital radiology digital construction of its problems, propose solutions.Results:The primary hospital exists in the construction of digital radiology department layout as unscientific, CR and DR choose unreasonable, machine design flaws, PACS incompatible, system maintenance improper and operation of the medical staff is not standardized, require planning, the management, design, training, etc. to further improve.Conclusion: Radiology digital construction is the inevitable trend of development, only reasonable choice, scientific installation, meticulously maintained, proficient in primary hospital and give full play to its advantages.

Objective To Study the supplementary effect of internet communication technologydoctor-patient communication information system in the health education of patients in hand surgery department. Methods We divided 200 outpatients as well as 200 inpatients in hand surgery department into the control group and the test group with 100 cases in each group.We gave health education to the test group by sending short messages using doctor-patient communication information system. The control group received health education by routine method.The incidence rate of complications after operation in the two groups of inpatients and two groups of outpatients was compared. Results Statistical difference existed between the test group and the control group of inpatients and the test group and the control group of outpatients. Conclusions The application of internet communication technology of doctor-patient communication information system in the health education for patients in hand surgery department could effectivelydecrease the incidence rate of complications after operation.

OBJECTIVE: To strengthen the management on procedures for the introduction,examination and approval of new drugs in hospital.METHODS: The experience on the current procedures for the introduction,examination and approval of new drugs in our hospital was summarized,and the Microsoft.NET technology was used to develop Web-based "information system of introduction,examination and approval of new drugs".RESULTS & CONCLUSIONS: The new system adopted three-level "browser / server" structural pattern and it is composed of external network new drug data collection system and internal network new drug examination and approval system.The system is advanced,stabile,practical,intelligent and safe,and it has simplified the new drug examination and approval procedure,enhanced the working efficiency.Its application is characterized by justice,publicity and transparency.

OBJECTIVE: To investigate the utilization and development trend of heat-clearing drugs(HCDs) in our hospital so as to provide references for rational drug use and reasonable drug purchase.METHODS: The consumption quantity and consumption sum of HCDs during the period 2006～2007 were investigated,and the DDDs and daily consumption sum of HCDs were analyzed using DDDs sequence analytical method.RESULTS: The consumption quantity of HCDs in the hospital during 2006～2007 showed a steady increase and the DDDs of HCDs increased synchronously.CONCLUSION: The utilization status of HCDs in the hospital is basically in consistent with national drug consumption tendency.

AIM: To establish an analytical method for detecting 14 kinds of amino acid in Wuling Capsule(xylaria) by HPLC-FLD. METHODS: After being derivated in precolumn with o-phthalaldehyde and ?-mercaptoethanol,the amino acids were eluted by gradient elution and were detected with a fluorescence detector (Ex = 340 nm,Em = 450 nm). The mobile phase consisted of 0. 1 mol/L NaH2PO4 ( adjusted to pH 6. 8 by phosphoric acid) and methanol with a flow rate of 1. 0 mL/min,column temperature was set at 30 ℃. RESULTS: The deriva-tives of 14 amino acids with o-phthalaldehyde could be separated with a stable base line within 40 min. The linear range of the detection was 5-200 nmol/mL and correlation coefficients were over 0. 999 0. CONCLUSION: The method is simple,accurate and well reproduced for the determination of amino acids in Wuling Capsule.

Objective To study biological significance of CD44v6 and p63 protein expression in buccal squamous cell carcinomas (BSCC). Methods Immunohistochemical method was used to examine the expression of CD44v6 and p63 protein in 52 cases of BSCC,10 cases of atypical hyperplasia and 10 cases of normal buccal mucosa,which were analyzed based on clinic and pathology characteristics.Results In normal tissue,atypical hyperplastic tissue of buccal mucosa and BSCC tissue,the expression of CD44v6 decreased gradually 90.0 ％ (9/10),80.0 ％ (8/10) and 55.8 ％ (29/52),respectively,and the layers of positive expression was decreased,and the expression of p63 increased gradually,30.0 ％ (3/10),40.0 ％ (4/10) and 90.4 ％(47/52),respectively.The differences of their expression rates were significant (P ＜0.05).The expression of CD44v6 and p63 had no relationship with gender,age,tumor classification,pathological differentiation (P ＞0.05).CD44v6 was relevant with invasion style and lymphatic node metastasis of BSCC (P ＜ 0.05),while p63 has no relevance with lymphatic node metastasis of BSCC (P ＞0.05).Conclusion CD44v6 and p63 participate in the regulation of generation and deve-lopment process of BSCC.CD44v6 can be utilized as a useful marker of BSCC for detection and prediction of prognosis.p63 play a role as oncogene,which can be a useful marker for BSCC detection.CD44v6 and p63 have independent function in generation,development,metastasis and aversion of BSCC.

0.997 5);their average recovery rates were 99.93%,99.82%,100.02%,99.03%,and 98.79%(n=9),respectively,and their RSD were 0.66%,0.42%,0.55%,0.74%,and 0.98%(n=6),respectively.CONCLUSION:The established method is simple,rapid with accurate and reliable results thus applicable for the quality control of lecithin.

Objective To investigate the role and feasibility of tumor necrosis factor(TNF) and/or interferon(IFN) in comprehensive treatment of tongue cancer in vitro and explore a new pathway about clinical treatment of tongue cancer . Methods The role of TNF and/or IFN in tongue cancer cell line (Tca 8113) growth and their effect on chemotherapy & radiotherapy of Tca8113 were detected by MTT-assay in vitro.Results ⑴There was a obvious volume-effect relationship in inhibitory effect of TNF or IFN on tongue cancer cell line; when TNF combind with IFN,their cytotoxity enhanced significantly than only TNF or IFN. ⑵TNF & IFN have enhanced the cytotoxities of low dose DDP,PLM and Taxol on Tca 8113 cell line.⑶TNF obviously enhanced the inhibition rate of radiotherapy(dosage:2～8Gy)on Tac 8113 cell line(P