1.STUDY ON SERUM LIPOPROTEIN CHOLESTEROL AND LIPID OF 1974 HEALTHY SUBJECTS AND 54 PATIENTS WITH CHD AND CVD
Luosheng WEI ; Lingjun LIU ; Xinnian ZHOU ; Ruduan WANG
Acta Nutrimenta Sinica 1956;0(02):-
The serum lipid and lipoprotien cholesterol levels of 1974 healthy subjects from newborn to 75 years of age and 51 patients with coronary heart disease (CHD) and cerebrovascular disease(CVD) were studied in Wuhan district. The serum lipid and lipoprotein cholseterol concentration varies with age. But no differences were found between male and female cord blood. In both sexes the serum T-C, LDL-C, HDL-C, T-C/HDL-C and LDL-C/ HDL-C ratio increased with the increasing of age. The serum HDL-C level has significant difference between male and female (P
2.Study of ?-defensin rBD-2 gene expression in the pulmonary tissues of the fetal, neonatal and adult rats
Hui ZHOU ; Ning HUANG ; Xinnian CHEN ; Qi WU ; Boyao WANG
Chinese Journal of Pathophysiology 1986;0(03):-
AIM: To investigate the developmental regulation of ?-defensin rBD-2 gene expression in the rat lung. METHOD: Total RNA was isolated from the pulmonary tissues of the fetal, neonatal and adult rats. RT-PCR were performed with primers (P 1: TTCAGTCATGAGGATCCATT AC; P 2: TGGAACTTGGTCTTTTTATCTAC). The RT-PCR products were cloned into pGEM-T easy vector and the recombinant plasmid was analyzed with EcoR1 digestion and the inserted DNA sequencing was performed on ABI PRISM-377 DNA sequencer. RESULTS: Rat ?-defensin-2 transcripts were detected in all the pulmonary tissues of rats during different developmental stages, e.g. at just before birth, 8 hours and 4 days after birth , and adult. CONCLUSION: The rat ?-defensin-2 is constitutively expressed in the pulmonary tissues, suggesting that ?-defensin-2 may play a role in the lung innate defense against infection.
3.Study of β-defensin rBD-2 gene expression in the pulmonary tissues of the fetal, neonatal and adult rats
Hui ZHOU ; Ning HUANG ; Xinnian CHEN ; Qi WU ; Boyao WANG
Chinese Journal of Pathophysiology 2001;17(3):226-229
AIM: To investigate the developmental regulation of β-defensin rBD-2 gene expression in the rat lung. METHOD: Total RNA was isolated from the pulmonary tissues of the fetal, neonatal and adult rats. RT-PCR were performed with primers (P1: TTCAGTCATGAGGATCCATT AC; P2: TGGAACTTGGTCTTTTTATCTAC). The RT-PCR products were cloned into pGEM-T easy vector and the recombinant plasmid was analyzed with EcoR1 digestion and the inserted DNA sequencing was performed on ABI PRISM-377 DNA sequencer. RESULTS: Rat β-defensin-2 transcripts were detected in all the pulmonary tissues of rats during different developmental stages, e.g. at just before birth, 8 hours and 4 days after birth , and adult. CONCLUSION: The rat β-defensin-2 is constitutively expressed in the pulmonary tissues, suggesting that β-defensin-2 may play a role in the lung innate defense against infection.
5. Ventilator-associated pneumonia among premature infants <34 weeks′ gestational age in neonatal intensive care unit in China: a multicenter study
Shujuan LI ; Weili YAN ; Qi ZHOU ; Shuping HAN ; Jinzhen GUO ; Shiwen XIA ; Shah VIBHUTI ; Sannan WANG ; Yong JI ; Changyi YANG ; Chuanzhong YANG ; Ruobing SHAN ; Ling LIU ; Bin YI ; Jiangqin LIU ; Zhenlang LIN ; Yang WANG ; Ling HE ; Mingxia LI ; Xinnian PAN ; Yan GUO ; Ling CHEN ; Cuiqing LIU ; Qin ZHOU ; Xiaoying LI ; Hong XIONG ; Yujie QI ; Mingyan HEI ; Yun CAO ; Siyuan JIANG ; Yi ZHANG ; K. Lee SHOO
Chinese Journal of Pediatrics 2017;55(3):182-187
Objective:
To investigate the incidence and pathogen distribution of ventilator-associated pneumonia (VAP) among preterm infants admitted to level Ⅲ neonatal intensive care units (NICU) in China.
Method:
A prospective study was conducted in 25 level Ⅲ NICU, enrolling all preterm infants <34 weeks gestational age admitted to the participating NICU within the first 7 days of life from May 2015 to April 2016. Chi-square test,