The investigation was conducted among eight untrained male and six well-trained male cyclists with the purpose of finding out the relationship between muscle fiber composition and lactate threshold. Muscle biopsy samples were taken of vastus lateralis to determine the composition of fiber type (Ⅰ, Ⅱ). Maximal oxygen uptake was measured in a progressive cycle ergometer test, while blood lactate threshold was determined on the basis of a systematic increase in BLa above baseline warm-up values. Analyses show that the percentage of type Ⅰ is closely correlated with lactate threshold in both groups of subjects and that muscle fiber composition plays (?) important role in determining lactate threshold. Therefore, an assessment of muscle fiber composition is useful for spotting athletes with high potential for endurance performance.

Objective To design a novel medical infrared real-time imaging system based on uncooled focal plane assembly(UFPA).Methods The hardware includes optics,infrared detector,thermal image acquisition & pre-treatment circuit and portable computer.The software includes original image data acquisition and correction module,image real-time treatment and display module,pseudo-color treatment module and image storage and off-line analysis module.Two points revising method was proposed aiming at the unevenness of pixel.Results Dispensing with refrigeration,the resolution of medical infrared real-time imaging system was 0.1℃.The measuring temperature ranged from-20℃ to 100℃ with ambient temperature ranging from 0℃～40℃.The real-time imaging speed was 25 frame/s.Conclusion The medical infrared real-time imaging system can form infrared image in real-time,which meets the requirement of clinical application.

Objective To observe the imaging features of primary hepatic endocrine carcinoma. Methods Three patients with primary hepatic endocrine carcinoma proven pathologically were retrospectively analyzed. Results Single liver lesion was detected in all 3 patients, 2 in right and 1 in left lobe of liver. The maximum diameter of the masses was 4.8 cm, 6.7 cm and 10.0 cm, respectively. The masses were all solid with different extent of low density. The solid part enhanced greatly in contrast enhanced CT scanning, while the non-solid part did not. The bigger lesions pushed the vascular to move aside in 2 patients. Enhanced and circuitous vascular was observed in 1 lesion. Slightly low signal was noticed on MR T1WI , while high signal was found on T2WI and DWI. Conclusion CT and MR can show specific features of primary hepatic endocrine carcinoma, i.e. usually single solid mass with various low densities inside and the solid part enhancing dramatically.

Objective To observe the role of PGE 1 in heart-lung transplantation. Methods PGE 1 combined with modified LPD solution as the lung flush solution of the donor was applied in heart-lung transplantation. Results PGE 1 combinded with modified LPD solution provided the excellent effect of lung pretection. The patient survived over one year after heart-lung transplantation, and never occurred severe infection, lung disfunction and severe graft rejection. Conclusion The combination use of PGE 1 and modified LPD solution as the lung flush solution in heart-lung transplantation possessed a excellent protective effect for lung.

Objective To compare myocardial protection between cold blood and crystalloid cardioplegia in short aortic cross-clamping(

Objective To review the experience of closure of recalcitrant bronchopleural fistula using the transsternal transpericardial approach. Methods 3 patients with recalcitrant bronchopleural fistula underwent transsternal transpericardial closure of a bronchopleural fistula and drainage by open thoracostomy. Results All patients were survived and there was no recurrence of bronchopleural fistula. Conclusion Transsternal transpericardial approach seems to be a safe and effective method in cases of bronchopleural fistula complicated with empyema. It has the added advantage of less recurrent fistula formation.

Background and purpose:Exploration of the effective early diagnostic and prognostic markers of non-small cell lung cancer (NSCLC) has important scientiifc signiifcance and clinical value. Recently, the role of long chain non-coding RNA (lncRNA) in the tumor attracts widespread attention. This study intended to investigate the level of lncRNA HOTTIP in NSCLC, the effect of HOTTIP on cell proliferation and its mechanisms.Methods:Expression of lncRNA HOTTIP in tumor and their matched non-tumor tissues were determined by quantitative real-time PCR (qRT-PCR) in NSCLC patients. Then, we analyzed the potential correlation of lncRNA HOTTIP expression levels in tumor tissues with clinicopathological features of NSCLC and clinical outcome. The effects of HOTTIP on NSCLC cell proliferation and apoptosis were tested usingin vitro MTT and lfow cytometric assays. Western blot method was uesd to detect the expressions of proteins.Results:LncRNA HOTTIP expression level was signiifcantly decreased in NSCLC tissues in comparison to adjacent non-tumor tissues (P<0.05). It was also proved that HOTTIP expression was associat-ed with NSCLC histological grade and lymph node metastasis. Moreover, knockdown of HOTTIP expression in A549 cell line decreased proliferation and enhanced apoptosis compared with transfected negative control. Western blot assay showed that the level of Bax protein was signiifcantly increased, whereas Bcl-2 was signiifcantly decreased in HOT-TIP-silencing A549 cell.Conclusion:HOTTIP is a novel prognostic biomarker and a potential therapeutic candidate forNSCLC.

Objective To evaluate the ability of diffusion-weighted imaging (DWI) and the apparent diffusion coefficient (ADC) value in diagnosis of pancreatic cancer at 3.0T MR. Methods The diffusion-weigted sequences with b=800 s/mm~2 were performed in 30 patients of pancreatic cancer confirmed pathology, and in 30 control subjects with normal pancreas as well. ADC value of the cancer, pericancerous tissue and normal pancreas were recorded and statistically analyzed with ANOVA and ROC analysis. Results The mean ADC value of cancer, pericancerous tissue and normal pancreas was(1.494±0.273)×10~(-3) mm~2/s, (1.631±0.281)×10~(-3) mm~2/s and (1.778±0.237)×10~(-3) mm~2/s, respectively. ADC value of pancreatic cancer was significant different from that of the normal pancreas, while there was no significant difference between pancreatic cancer and pericancerous tissue. The one-side upper limit of 95% confidence interval of mean ADCs (1.622×10~(-3)mm~2/s)was adopted as the point to diagnosis of pancreatitis. The area under ROC curve was Az=0.800. Conclusion ADC values are helpful to differential diagnosis of pancreatic cancer and normal pancreas. DWI may be do some contribution to the diagnosis of pancreatic cancer.

Objective To quantify the perfusion parameters of normal pancreas and pancreatic carcinoma with three-dimension (3D) fast spoiled gradient echo dynamic contrast enhanced (DCE) MRI on 3.0T MR system, and to assess the value of 3D DCE-MRI in the diagnosis of pancreatic carcinoma. Methods Thirty-four patients with pathology verified pancreatic carcinoma and 31 control subjects with normal pancreas (without pancreatic diseases) underwent DCE-MRI with 3D LAVA sequence of ten phases. The data were processed on ADW 4.2 workstation. The perfusion parameters of the head, body and tail of normal pancreas, together with lesion and non-lesion area of pancreatic carcinoma were measured and statistically analyzed, including signal enhancement ratio at 30 s after injection (SER_(30)), signal enhancement ratio at 90 s after injection(SER_(90)), positive enhancement integral (PEI), time to peak (TTP) and maximum slope of increase (MSI). Results There was no significant perfusion difference among head, body or tail of normal pancreas (P>0.05). The difference of SER_(30), PEI, TTP and MSI between lesion and non-lesion region of carcinous pancreas was significant (P<0.05). The TTP between normal pancreas and the non-lesion region of carcinous pancreas was significantly different (P<0.05). Conclusion Normal pancreas has no regional perfusion difference. The data from DCE-MRI provide reliable information for the diagnosis of pancreatic cancer, and for the assessment of the invasion of pancreatic carcinoma. The difference in TTP between the normal pancreas and non-lesion region of carcinous pancreas suggest the existing of potential lesions.

Objective To study the proper desiccation and the expiry date for humidifier after disinfection, and then decline the incidence rate of infection in hospital. Methods Divided 84 humidifiers into the A and B groups randomly after disinfection, there were 42 humidifiers in the each group. The natural desiccation was used in the A group, the oxygen desiccation was used in the B group. Monitored the humidifiers of the two groups respectively lasted 7 days, and then compare the datum between them. Results The expiry date of the humidifiers which have dried by natural desiccation was longer than that of humidifiers which have dried by oxygen desiccation. Conclusion We should set down the expiry date for humidifier according to the different desiccation and the different seasons.