In order to make early diagnosis of the ischemic heart disease in the aged NIDDM patients. 500 patients underwent a series of examinations including maximal plate exercise with 59M Tc-MIBI ECT myocardiography. maximal plate exercise ECG and 24-hour ambulatory ECG. The serum lipid. glucose and insulin levels, daily urine protein, glomerular filtration rate,body weight index and eyeground were also studied. The results showed that 56 patients had obvious myocardial ischemia (group A),49 patients had slight myocardial ischemia (group B)and 395 patients were free from myocardial ischemia (group C). With positive 59MTc-MIBI ECT myocardiography, 15 among 16 patients in group A and 6 among 12 patients in group B and none among 5 in group C showed coronary artery narrowing in coronary radiography. The average course of DM,serum total cholesterol,fasting serum glucose and insulin, glucosylated hemoglobin.daily urine protein and the incidence of proliferative retinopathy of group A were higher than those of group B or C(P

Objective To evaluate the value of the peripheral capillary fasting plasma glucose examined by glucometer on screening of type 2 diabetes.Methods With the proportion stratified group sampling,all residents aged 15-75 years in Wuqing district were selected and detected on their capillary fasting plasma glucose(CFPG) by clucometer,and those whose CFPG was higher than 5.0mmol/L should have a 2-hour postprandial glucose(2 h PG) test.Data were analyzed by SPSS 11.5 software.Results The detection rates of diabetes,impaired glucose tolerance and impaired fasting blood glucose were 42.83%,27.88% and 3.74%,respectively.Making two ROC curve with test variable(FPG) and state variables(2 h PG≥11.1 mmol/L and 7.8 mmol/L≤2 h PG≤11.0 mmol/L),the areas under the curve were 0.862 and 0.405,respectively.Conclusion It is suggested to use the sensitivity standard that nears to 99% FPG≥5.0 mmol/L to screen diabetes.

Objective To study the expression of collagenase Ⅳ and collagen type Ⅳ in papillary thyroid carcinoma (PTC). Methods Immunohistochemical staining(SP method)was used to detect the expression of collagenase Ⅳ and collagen typeⅣ. Results ⑴The positive expression rate of collagenase Ⅳ was 77 9%, 66 7%, 80 0%, 88 9% and 25 4% in PTC, papillary microcarcinoma, intra-thyroid, extra-thyroid carcinoma and tissues adjacent to tumor(P

Objective To study the effect of variant concentrations of lidocaine on voltage-dependent sodium current in dissociated hippocampal neurons of the ratMethods Voltage-dependent sodium currents in dissociated hippocampal neurons of the rat were recorded by whole-cell configuration of the patch clamp when a series of doses of lidocaine (10 -5mol/L-10 -2mol/L) were appliedResults Lidocaine reduced the amplitude of sodium current in a dose-dependent manner, with EC50 of (039?005)mmol/LConclusions Voltage-dependent sodium channel is related to cerebral protective effect of lidocaine, but has nothing to do with its nervous toxicity

Objective To observe the time profile of the changes of mRNA of ? and ? subunit of acetylcholine receptor (AChR) in skeletal muscle and the level of calcitonin gene related peptide (CGRP) in spinal motoneuron after upper motoneuron injury. Method The spinal cord of adult male Wistar rats was transected on the lower thoracic segment. The spinal cords below the transection level and soleus muscles were sampled 0,1,2,4,7 and 14 days after surgery, respectively, to detect the levels of mRNA of ? and ? subunit of acetylcholine receptor in soleus muscle using Northern blot method,and CGRP level in spinal motoneuron using radioimmunoassay.Results The levels of mRNA of ? and ? subunit of acetylcholine receptor siginificantly increased after surgery, but the level of mRNA of ? subunit increased earlier and higher than ? subunit. The level of CGRP in spinal motoneuron markedly reduced after surgery, was maintained at 50 80% of normal level since the 2nd day after surgery. Conclusions The levels of AChR ? and ? subunit in skeletal muscle significantly increase after upper motoneuron injury, the expression of which CGRP may regulate.

Objective To assess the changes in coagulation induced by acute normovolemic hemodilution Methods Twenty ASA Ⅰ Ⅱ patients scheduled for elective surgery were divided into two groups: HES group and GEL group in which hydroxyethyl starch(HES) or succinylated gelatin (GEL) was used as the hemodilution fluid Patients with coagulation abnormalities or kidney dysfunction were excluded from the study Anesthesia was induced with propofol,fentanyl and vecuronium and maintained with inhalation of isoflurane and nitrous oxide and intermittent intravenous boluses of fentanyl and vecuronium The patients were mechanically ventilated and PaO 2 and PaCO 2 were maintained within normal range After induction blood was withdrawn from internal jugular vein and collected in standard blood bags containing anticoagulant and at the same time replaced with HES (equal volume) or GEL (1 5 times the volume of blood withdrawn) The amount of blood withdrawn was estimated from the following equation:blood volume withdrawn=body weight(kg)?60?(Hct 0 Hct 1)/Hct M,where Hct 0=patient′s initial Hct, Hct 1=desired end point Hct and Hct M=the average of Hct 0 and Hct 1 Blood routine,prothrombin time(PT),activated partial thromboplastin time(APTT) and fibrinogen level were checked and thromboelastography(TEG) was performed 15 min before and after hemodilution Results The amount of blood withdrawn ranged between 1000 1100ml Hb was still above 80g/L after hemodilution The decreases in Hb and Hct were significant in both groups (P

Objective To compare the differences of self-imposed delay of gratification and the characteristics of delay strategy between aggressive children and normal children, in order to provide evidence-based basis for aggressive children's education and behavior modification. Methods Using the classical self-imposed delay of gratification task to compare the ability of delay of gratification between 24 aggressive children and 24 class-, age-, gender-matched normal controls. Recording children' s delay behavior coding to compare their differences of delay strategy by using the time sampling observation method. The differences of purpose of delay of gratification was analyzed with χ2 test. The differences of their delay strategy was analyzed with the independent sample t -test. Results A significant difference was found between two groups on delay of gratification(χ2 = 13. 50, P ＜0.01). The controls showed more purpose during the waiting process than the aggressive children whose waiting was mostly without purpose or they simply gave up. The problem-solving strategy was hightlighted in the use of delay strategy for both the aggressive children(22. 38 ±11. 29) and the controls( 28. 21 ± 3. 05). The control group children used more problem-solving strategy(28.21 ±3.05) and self-control strategy(0. 71 ± 1.08) during the waiting task. Their level of delay strategy was higher than the aggressive groups ( (22. 38 ± 11. 29), (0. 13 ± 0. 45) ) (P ＜ 0. 05). Conclusion The aggressive children are less capable of self-imposed delay of gratification,the purpose of behavior is weaker and the level of delay strategy is lower than normal children.

Objective To study the effect of individual and environmental factors of diabeties on their glycemic and lipidic metabolism. Methods While educating diabeties with diabetic informations, we inguired their individual data questionnaires. According to their diabetic periods,cultural levels,compliance to their doctors,daily work times and ocupations respectively,we grouped these patients and compared their fasting and postprandial glycemia (FBG & PBG), GHb,serum lipids and serum uric acid (BUA). Results Among 2 856 diabetic patients,the type I was about 4. 9% and the type Ⅱ about 95. 1% ; the family patients was about 9. 91% and the overweight about 44. 29K. According to patients' compliance to their doctors,blood glucose,GHb and lipids and BUA was lower in absolute compliance group than the basical group or the non compliance one (P＜0. 02),and the levels of glycemia and GHb and TG all related to their compliance (respectively r=- 0. 059, - 0. 055, - 0. 075, - 0. 063 ; and P＜0. 05, 0. 05, 0. 01, 0. 025 ). Considering the diabetes periods,the 1-5 year metabolic control of group was best (P＜0. 02) and the 5- 10 year and above 10 year metabolic control groups were better than the group within one year (P ＜0. 05). To patients' cultural levels, the metabolic control senior or higher middle school groups was better than the junior middle school or lower (P＜0. 05). Thinking their daily work time and occupation,the metabolic control of groups working more than seven hours was worst (p respectively less than 0. 05 and 0. 02). Conclusion diabetic patients' individual and environmental factors are all affecting their metabolic control,among them the complicance is most effective.

Objective: To investigate the tendency and correlation between connective tissue growth factor (CTGF) and low density lipoprotein receptor-related protein (LRP) in human pulmonary fibroblasts-1 (HPF-1) induced by transforming growth factor beta1 (TGF-?_1) at different times. Methods: After the HPF-1 cells were stimulated with TGF-?_1 (5 ?g/L) at different times (0 h, 3 h,6 h,12 h and 24 h),CTGF and LRP mRNA expressions were analyzed by RT-PCR. The same preparation steps of cell culture were repeated, then, the protein expressions were determined by Western Blot, co-immunoprecipitation Western Blot and immunocytochemistry. Results: The expressions of CTGF and LRP mRNA had similar tendency, and LRP (224.87?7.00) correlated with CTGF (131.53?2.86) positively (r= 0.8402, P

Objective To evaluate the effect of blocking the posterior branc h of lumbar nerves in treatment of the lower lumbago and scelalgia caused by the i njury of the posterior branch of lumbar nerves. Methods 560 cases with lower lum bago and scelagia caused by the injury of the posterior branch of lumbar nerves were randomized into the treating group and the contrast group. Follow-ups were done 2 to 3 months after the treatment. Then the effects of the treatment were evaluated. Results The effective rate of the treating group was 98. 0 %,and th e excellect and good rate was 83.0 %; the effective rate of the contrast group was 83.3 %,and the excellect and good rate was 64.9 %. The differences in tre atment effects between the two groups are statistically significnt (P