The present article deals with the result of early repair of 105 cases of cleft palate within the first two years of life.The result is excellent without fistulae,ruptures or deaths.The early repair of the cleft palate has the advantages of small blood loss during operation,little postoperative reaction and better healing.The key points of surgical procedure and postoperative management are introduced in details.The safety of the early operation and its influence on the development of maxillofacial growth are also discussed.The deficiency of maxillofacial growth is related to surgical procedures of cleft lip and palate besides the congenital factors.Early repair of cleft palate enables the child to develop normal speech and is advantageous to psychological development of the child.

HIV-1 utilizes cellular factors for efficient replication. The viral RNA is different from cellular mRNAs in many aspects, and is prone to attacks by cellular RNA quality control systems. To establish effective infection, the virus has evolved multiple mechanisms to protect its RNA. Here, we show that expression of the Y-box binding protein 1 (YB-1) enhanced the production of HIV-1. Downregulation of endogenous YB-1 in producer cells decreased viral production. YB-1 increased viral protein expression by stabilizing HIV-1 RNAs. The stem loop 2 in the HIV-1 RNA packaging signal was mapped to be the YB-1-responsive element. Taken together, these results indicate that YB-1 stabilizes HIV-1 genomic RNA and thereby enhances HIV-1 gene expression and viral production.
5' Untranslated Regions ; Chromosome Mapping ; Down-Regulation ; HEK293 Cells ; HIV-1 ; genetics ; metabolism ; Humans ; Inverted Repeat Sequences ; Protein Binding ; RNA Interference ; RNA, Small Interfering ; metabolism ; RNA, Viral ; metabolism ; Virus Replication ; Y-Box-Binding Protein 1 ; antagonists & inhibitors ; genetics ; metabolism