1.The study of the influence of nasal feeding tube diameter on the incidence of pulmonary infection in acute stroke patients
Xiaohua PAN ; Aiqiong CHENG ; Xinlei MAO ; Weidong CHEN
Parenteral & Enteral Nutrition 1997;0(01):-
0.05),but it in thin nasal feeding tube group was significantly lower than that in the other two groups(P
2.Differentiation of Non-puerperal Mastitis from Breast Carcinoma with Non-mass-like Enhancement by Texture Analysis of Contrast-enhanced Magnetic Resonance Imaging
Honsum LI ; Qiang ZHANG ; Haikun QI ; Xinlei PAN ; Sheng XIE ; Huijun CHEN
Chinese Journal of Medical Imaging 2017;25(5):354-359
Purpose To investigate the feasibility of texture analysis of breast contrastenhanced magnetic resonance imaging in differentiating non-puerperal mastitis and breast carcinoma with non-mass-like enhancement in order to prevent misdiagnosis of nonpuerperal mastitis.Materials and Methods In this retrospective study,the contrastenhanced MRI images of 42 female patients of invasive ductal carcinoma with non-masslike enhancement and 30 female patients of non-puerperal mastitis were analyzed.3234 texture features were generated from manually selected region of interest (ROI) of normal breast tissue and breast lesions.By means of genetic algorithm and linear discriminative analysis,10 texture features were selected based on their stability and accuracy in breast tissue classification.Results With these 10 features,the linear discriminative analysis classifiers had sensitivity of 92.9% and specificity of 90.0% in classifying two lesions,and accuracy of 89.6% in classifying all three types of tissue.The result showed that texture analysis successfully differentiate non-puerperal mastitis and breast carcinoma with nonmass-like enhancement.Conclusion Texture analysis demonstrates the ability of differentiating invasive ductal carcinoma with non-mass-like enhancement,non-puerperal mastitis and normal breast tissue,and provides reliable results for clinical diagnosis.
3.Effect of hydrogen on mitochondrial membrane potential during focal cerebral ischemia-reperfusion in rats
Xinlei ZHANG ; Pan CHEN ; Yuning XIA ; Xuemei LI ; Yongxing TAN
Chinese Journal of Anesthesiology 2018;38(5):598-601
Objective To investigate the effect of hydrogen on mitochondrial membrane potential during focal cerebral ischemia-reperfusion (I/R) in rats.Methods Eighty-four healthy adult male SPF Sprague-Dawley rats,weighing 220-240 g,were divided into 3 groups (n=28 each) using a random number table:sham operation group (Sham group),I/R group and hydrogen group (H2 group).Focal cerebral I/R was produced by mid-cerebral artery occlusion in I/R and H2 groups.Hydrogen 10 ml/kg was intraperitoneally injected immediately after onset of reperfusion and at 12 h of reperfusion in group H2.Neurological deficit was scored at 24 h of reperfusion.The rats were then sacrificed and brain tissues in cortex were removed for microscopic examination of the pathological changes and for determination of cerebral infarct size (by TFC staining),nerve cell apoptosis (by TUNEL),mitochondrial membrane potential (by JC-1 staining) and expression of Bcl-2 and Bax (by Western blot).Apoptotic index (AI) was calculated.Results Compared with Sham group,the neurological deficit score,percentage of cerebral infarct size and AI were significantly increased,the mitochondrial membrane potential was decreased,Bax expression in brain tissues was up-regulated,and Bcl-2 expression in brain tissues was down-regulated in I/R and H2 groups (P<0.05).Compared with I/R group,the neurological deficit score,percentage of cerebral infarct size and AI were significantly decreased,the mitochondrial membrane potential was increased,Bax expression in brain tissues was down-regulated,and Bcl-2 expression in brain tissues was up-regulated (P<0.05),and the pathological changes of brain tissues were significantly attenuated in H2 group.Conclusion The mechanism by which hydrogen reduces focal cerebral I/R injury is related to decreasing dissipation of mitochondrial membrane potential and inhibiting nerve cell apoptosis in rats.
4.Effect of hydrogen on mitochondrial function after cerebral ischemia/reperfusion injury in rats
Xinlei ZHANG ; Pan CHEN ; Yuning XIA ; Xuemei LI ; Chunguang DAI ; Yongxing TAN
The Journal of Practical Medicine 2018;34(2):184-187
Objective To study the effect of hydrogen on mitochondrial function in cerebral cortex after cerebral ischemia/reperfusion(I/R)injury in rats. Methods 48 male SD rats were randomly divided into sham operation group(sham group),brain I/R injury group(MOD group),hydrogen treatment group(H2group). 24 hours after reperfusion,the neurological deficit scoring was performed. The changes of mitochondrial membrane potential(△ψm),permeability openness(MPTP),ROS production rate and mitochondrial swelling were detected. Results Compared with the Sham group,neurological deficit score,MPTP openness,mitochondrial swelling degree and ROS production rate were increased in the MOD group(P<0.01),△ψm levels were reduced(P<0.01). Compared with the MOD group,the neurological deficit score,MPTP openness,mitochondrial swelling degree and ROS production rate were decreased in H2group(P<0.01),△ψm levels increased(P<0.01). Conclusions Simul-taneous intraperitoneal injection of pure hydrogen can reduce the generation of reactive oxygen species,protect the mitochondrial function of neuronal cells in the ischemic region after brain I/R,improvement the rat brain I/R after the neurological scoring.
5.Simultaneous Determination of 7 Effective Components in Yao Medicine Yueli Yaomi Spray by QAMS
Jingzhi ZHANG ; Danqing ZHAO ; Zhengrong WANG ; Jinyan JIA ; Lin YANG ; Shengsheng ZHANG ; Xinlei LIU ; Qing WU ; Wei PAN
China Pharmacy 2020;31(9):1044-1049
OBJECTIVE:To establish QAMS method for simultaneou s determination of 7 effective components in Yao medicine Yueli yaomi spray ,such as α-cyperone,α-pinene,β-pinene,limonene,β-elemene,caryophyllene oxide and ligustilide , so as to provide method reference for the quality control of the preparation. METHODS :GC method was adopted. The determination was performed on DB- 1701P capillary column ,using nitrogen as carrier gas. The temperature of the hydrogen flame ion detector was 240 ℃. The temperature was programmed ,the inlet temperature was 240 ℃,the injection volume was 1 μL and the split ratio was 20 ∶ 1. Using limonene as internal reference ,the relative correction factors of other 6 components were calculated,the contents of them were calculated with relative correction factors ,and then compared with the results of internal standard method (using naphthalene as internal standard ). RESULTS :The mass concentration linear range of α-cyperone, α-pinene,β-pinene,limonene,β-elemene,caryophyllene oxide and ligustilide were 0.008 9-1.110 0,0.028 3-3.540 0,0.020 5- 2.560 0,0.023 0-2.880 0,0.016 3-2.035 0,0.013 1-1.640 0,0.008 3-1.040 0 mg/mL(all r>0.999 0);the limits of quantification were 0.005 6,0.013 1,0.011 4,0.018 6,0.010 8,0.008 9,0.004 5 mg/mL;the detection limits were 0.001 9,0.004 1,0.003 7, 0.006 2,0.003 5,0.002 9,0.001 5 mg/mL;RSDs for precision ,stability(24 h),and repeatability tests were all less than 2% (n=5 or n=6); the average recoveries were 98.48% , 014) 101.37%,97.96%,99.80%,102.79%,97.77%,102.14%, and RSDs were all lower than 2%(n=9),respectively. The average relative correction factors of α-cyperone,α-pinene, β-pinene,β-elemene,caryophyllene oxide and ligustilide were 1.045 8,0.621 0,0.488 5,0.382 9,0.708 9,0.956 9 respectively,and the RSDs were all lower than 2%(n=6). There wa s no statistical significance in contents of 7 components between QAMS method and internal standard method (P>0.05). CONCLUSIONS :The established QAMS method is simple , accurate,stable and reproducible ,and can be used for simultaneous determination for 7 components in Yueli yaomi spray.