1.Simultaneous Determination of Cyclohexane,Cyclohexanone and Cyclohexanol in Workplace Air by Gas Chromatography
Xining WANG ; Xia ZHANG ; Yan ZHANG
Journal of Environment and Health 2007;0(07):-
Objective To establish a simultaneous determination method of cyclohexane,cyclohexanone and cyclohexanol in workplace air. Methods To collect the cyclohexane,cyclohexanone and cyclohexanol from the workplace air by pumping the air through the active carbon tube,resolve them with alcohol and then test the content of them by capillary gas chromatography. Results It was proved that these samples were separated effectively. The final measurement range of cyclohexane,cyclohexanone and cyclohexanol was 30-1 000 ?g/ml,20-1 000 ?g/ml and 20-880 ?g/ml respectively,the linear equation was y=257.4x+987.1 (r=0.999 9),y=239.9x+664.6 (r=0.9999 ) and y=192.4x+607.1 (r=0.999 9) respectively,the detection limit of was 1.5 ?g/ml,0.5 ?g/ml and 1.0 ?g/ml respectively. The minimum detectable concentration was 1.0 mg/m3,0.3 mg/m3 and 0.7 mg/m3 respectively (collected 1.5 L air sample). The efficiency of resolution was 90.1%,91.2% and 93.1% respectively. Conclusion The method is suitable for the simultaneous determination of the cyclohexane,cyclohexanone and cyclohexanol in the workplace air.
2.Clinical Effect and Mechanism of Modified Danshenyin Against Chronic Atrophic Gastritis with Syndrome of Stasis in Stomach Collateral: Based on Theory of Collateral Diseases
Defang WANG ; Ming ZHAO ; Xiaoyi DONG ; Xiaoyan LI
Chinese Journal of Experimental Traditional Medical Formulae 2022;28(23):122-127
ObjectiveTo observe the clinical effect of Danshenyin on chronic atrophic gastritis and explore the mechanism. MethodA total of 152 patients with chronic atrophic gastritis who were treated in Xining No. 1 People's Hospital were selected and randomized into control group (76 cases) and observation group (76 cases). The control group was given conventional western medicine treatment, and the observation group was given Danshenyin (oral). The treatment lasted 8 weeks for both groups. The clinical effect, traditional Chinese medicine (TCM) syndrome score, and pathological score of gastric mucosa were compared between two groups. The content of pepsinogenⅠ (PGⅠ), pepsinogenⅡ (PGⅡ), proinflammatory cytokines [soluble interleukin-2 receptor (sIL-2R), tumor necrosis factor-α (TNF-α)], and gastric mucosa-proteting factors [prostaglandin E2 (PGE2), calcitonin gene-related peptide (CGRP)] was determined, and the adverse reactions and recurrence rates were recorded. ResultThe effective rate (91.18%, 62/68) in the observation group was higher than that (7.61%, 52/67) in the control group (χ2=4.727, P<0.05). The TCM syndrome score in the observation group was lower than that in the control group (P<0.05). Compared with the control group, the observation group showed low pathological scores of glandular atrophy and intestinal metaplasia and dysplasia (P<0.05). The observation group had higher serum PGⅠ content and PGⅠ/PGⅡ value and lower PGⅡ content than the control group (P<0.05). The content of sIL-2R and TNF-α decreased (P<0.05) and that of PGE2 and CGRP increased (P<0.05) in the observation group as compared with those in the control group. The incidence of adverse reactions in the observation group was 7.35% (5/68), as compared with the 14.93% (10/67) in the control group. The recurrence rates in the 6-month and 12-month follow-up in the observation group were lower than those in the control group, but the difference was not statistically significant (Fisher's exact test). ConclusionDanshenyin shows satisfactory effect in relieving TCM syndrome, regulating PG secretion, and improving gastric mucosal lesions in the treatment of chronic atrophic gastritis. The mechanism is the likelihood that it modulates sIL-2R, TNF-α, PGE2, and CGRP to alleviate inflammatory damage of gastric mucosa and protect gastric mucosa. The medicine is safe and reduces the recurrence of chronic atrophic gastritis.
3.Efficacy of Therapy with Ginkgo Leaf Extract and Diphyridamole Injection for Chronic Kidney Diseases.
Jing SUN ; Xining WANG ; Rong WANG ; Kezhou YU ;
Chinese Journal of Practical Internal Medicine 2006;0(S1):-
Objective To evaluate the effect s of Ginkgo Leaf Extract and diphyridamole injection on patient s with CKD.Methods 60 patients with CKD were made up with 36 cases in Ⅲ phase and 24 cases in Ⅳ phase.The patients with CKD were given Ginkgo Leaf Extract and diphyridamole injection,20 mL/d,for 15 days.The parameters of clini- cal condition,function of kidney,ALB and blood fat were observed before and after the therapy and the data were ana- lyzed.Results There were 28 cases that clinical condition was better; 24cases no difference; and 8 cases worse.The level of TC and TG were significantly decreased after the therapy,as well as the uric protein quality of 24 hours(P0.05). Conclusion Essential treatment for CKD combined with Ginkgo Leaf Extract and diphyridamole injection can improve nutrition,delay the process of CKD and protect the renal function in patient s with Ⅲ phase.
4.High-density seeding affects transforming growth factor-beta1-induced epithelial-mesenchymal transition of HaCaT cells
Dianbao ZHANG ; Zhe WANG ; Ping SHI ; Xining PANG
Chinese Journal of Tissue Engineering Research 2013;(28):5191-5197
BACKGROUND: The cel density is one of the factors involved in the state of cel differentiation, and the effect of cel density on transforming growth factor-β1-induced epithelial-mesenchymal transition of HaCaT cells is stil unclear. OBJECTIVE: To observe the effect of cel density on transforming growth factor-β1-induced epithelial-mesenchymal transition of HaCaT cells. METHODS: HaCaT cells was seeded in 6-wel plates at low density of 103/cm2 and high density of 105/cm2 then treated by 2 μg/L transforming growth factor-β1 for 48 hours, thereafter observed the changes in cel morphology. The transcription levels of epithelial cadherin, tight junction protein-1, vimentin, neuronal-cadherin were detected by real-time PCR, and expression levels of epithelial cadherin and vimentin were detected by Western blot. RESULTS AND CONCLUSION: Cel gap of HaCaT cells grew larger after treated with transforming growth factor-β1 for 48 hours, and cel morphology was long spindle rather than polygonal in low-density group, while in high-density group without obvious morphological changes. The real-time PCR showed that the transcriptions of epithelial cel marker epithelial cadherin and tight junction protein-1 were suppressed when compared with the control group (P < 0.05), and the decreasing deree in the high-density group was higher than that in the low-density group (P <0.05), mesenchymal cel marker neuronal-cadherin and vimentin were upregulated in the high-density group and the low-density group when compared with the control group (P < 0.05), and there were no significant differences between high-density group and low-density group. Western blot results verified the changes of neuronal-cadherin and vimentin expression level. These results suggest that the high seeding density can inhibit transforming growth factor-β1-induced epithelial-mesenchymal transition of HaCaT cells.
5.Chinese medicine needs the baptism of science.
Chinese Journal of Integrated Traditional and Western Medicine 2012;32(8):1014-1022
All branches of mature practical technologies of both Eastern and Western ancient cultures, such as astronomy, geography, calendar, agriculture, architectonics, medicine, and so on, possess their own scientific connotation, which were derived from gradual accumulation and repeated validation of practical experiences. The ancient Greek medicine has the advantage of easily receiving scientific 'baptism' (reformation). The ontology and logics in ancient Greek philosophy, served as the epistemological and methodological bases, could effectively promote the development of science. Therefore, following the rapid progress of natural sciences since the Renaissance of the West world, the ancient Greek medicine rationally received the scientific "baptism" and gradually transformed into "modern medicine". In recent years, an upsurge to study and reappraise the works of Galen, an outstanding doctor and philosopher of Roman Empire, was evoked to discover and illuminate the practical and historical values of ancient Greek medicine. In ancient times, the medical theories and clinical practice of both Greek medicine and Chinese medicine were quite similar to each other, and they separately produced particular merits of themselves. However, owe to lack in the support of natural philosophy in ancient China, the progress of Chinese medicine, with its original native qualities for thousands of years only showed increase of clinical experiences, rather than scientific reformation of its essences. Therefore, Chinese medicine should also receive scientific "baptism" as Greek medicine. Ebb tide and see the real gold. The valuable medical experiences of Chinese medicine can be picked up for wide application, and its great historical achievements can be revealed for later pondering.
Medicine, Chinese Traditional
6.Research advances in targeting autophagy to alleviate hepatic ischemia-reperfusion injury
Zhen LI ; Ke WANG ; Kaiqiang WANG ; Kexian YU
Journal of Clinical Hepatology 2022;38(1):210-214
Hepatic ischemia-reperfusion injury (HIRI) is a common clinical problem after hepatectomy and liver transplantation and is the main cause of liver dysfunction and liver failure after transplantation. In recent years, autophagy-mediated pathways have become a research hotspot in alleviating HIRI. Autophagy refers to the process in which a large number of substrates such as cytoplasm and damaged organelles are transported into lysosomes for digestion and degradation, so as to constantly renew, reshape, and reuse cells. This article summarizes the research advances in the mechanism of targeting autophagy to alleviate HIRI from the aspects of gene, protein, signaling pathway, inflammatory response, oxidative stress, and mitochondrial and endoplasmic reticulum stress, as well as existing problems and prospects in research, in order to provide theoretical support for the future research on alleviating HIRI by targeting autophagy.
7.Investigation on anxiety and depression of employees in municipal and above administrative organs and public institutions in Qinghai region
Huaihong A ; Lihua WANG ; Dongsheng LIAO ; Hui TAN ; Shuang CHAI ; Yongxia MA ; Peiyao LIU ; Sina HAN ; Shujuan LUO ; Haixia LI ; Chunming BAI ; Yankun CHAI
Sichuan Mental Health 2024;37(5):464-470
BackgroundThe mental health problems of employees in administrative organs and public institutions are highly valued. However, there is a lack of understanding regarding the mental health status of employees in provincial and municipal administrative organs and public institutions in Qinghai region. ObjectiveTo analyze the anxiety and depression of employees in provincial and municipal administrative organs and public institutions in Qinghai region, so as to provide references for promoting their mental health and intervening in psychological problems. MethodsFrom October 23 to 28, 2022, a total of 3 096 employees in provincial and municipal administrative organs and public institutions in Qinghai region were enrolled using stratified cluster sampling technique, and were assessed using Self-rating Anxiety Scale (SAS) and Self-rating Depression Scale (SDS). ResultsA total of 1797 (58.04%) employees in municipal and above administrative organs and public institutions in Qinghai region completed a valid questionnaire survey. Depressive symptoms were noted in 579 (32.22%). SDS scores yielded statistical difference among the selected employees of different ethnicities, types of job positions and educational levels (F=9.074, 101.488, 4.477, P<0.01), and the severity of depressive symptoms also demonstrated statistical difference among the selected employees with different ethnicities, educational levels and types of job positions (H=49.196, 17.028, 160.848, P<0.01). Anxiety symptoms were detected in 711 (39.57%) employees. SAS scores exhibited statistical difference among the employees with different genders, ages, ethnicities, educational levels, marital status and types of job positions (t=-4.571, F=6.648, 3.950, 5.243, 12.008, 22.253, P<0.01). Statistical differences were also illustrated in the severity of anxiety symptoms among the selected employees with different ages, genders, ethnicities, educational levels, marital status and types of job positions (H=24.361, -3.788, 28.365, 24.268, 25.976, 56.204, P<0.01). ConclusionAbout one-third of employees in municipal and above administrative organs and public institutions in Qinghai region may have symptoms of depression and anxiety. The depression symptoms are more severe among Tibetan people, those with low education levels and ordinary civil servants. The anxiety symptoms are more severe among employees aged 30 and below, those with low education levels, unmarried and those in technical positions [Funded by 2020 Guiding Plan Project for the Health System of Qinghai Province (number, 2020-wjzdx-71)]
8.Effect of Jingui Shenqiwan Combined with Fracture Three-stage Syndrome Differentiation on Recovery of Patients with Osteoporotic Vertebral Compression Fractures After Operation
Bao-ming TANG ; Zhao-wei LI ; Ai-rong YANG ; Zhuo-ya WANG
Chinese Journal of Experimental Traditional Medical Formulae 2020;26(20):105-110
Objective:To evaluate the short-term and long-term efficacy of Jingui Shenqiwan combined with three-stage syndrome differentiation on osteoporotic vertebral compression fractures (OVCFs) after operation, and its regulatory effect on biomarkers of bone metabolism. Method:One hundred and thirty-five patients were randomly divided into control group (67 cases) and observation group (68 cases) by random number table. A total of 58 patients in control group completed the treatment (4 patients were exfoliated, 2 patients were lost to follow-up, 3 patients were eliminated); and 60 patients in observation group completed the treatment (3 patients were exfoliated, 2 patients were lost to follow-up, 3 patients were eliminated). Both groups patients were given calcitonin injection through intramuscular injection, 20 u/time, 1 time/week, for 12 weeks, calcium carbonate D3 chewable tablets (Ⅱ), 1 tablet/time, 2 times/day, and alendronate sodium tablets, 70 mg/time, 1 time/week. Patients in control group got Bushen Jiangu capsule, 4 grains/time, 3 times/day. And patients in observation group got modified Jingui Shenqiwan combined with fracture three-stage symptom differentiation, 1 dose/day. The courses of treatment in the two groups were 24 weeks, and a 24 week follow-up was provided. Before the operation and at the 12th and 24th week after operation, the short-term efficacy indexes, such as back pain, lumbar function, traditional Chinese medicine (TCM) syndromes and complications, were recorded. And the long-term efficacy indexes, such as recovery of responsible vertebral body, lumbar function, bone density and quality of life and incidence of 48 week re-fracture, were also recorded. Before and after operation, levels of bone alkaline phosphatase (BALP), osteocalcin (BGP), tartrate resistant acid phosphatase-5b (TRAP-5b), type I collagen carboxy terminal prepeptide (PICP), type I collagen cross-linked C-terminal peptide (
9.The expression of the sperm-specific lactate dehydrogenase gene Ldh-c in plateau pika (Ochotona curzoniae) cardiac muscle and its effect on the anaerobic glycolysis.
Xiao LI ; Lian WEI ; Yang WANG ; Li-Na XU ; Lin-Na WEI ; Deng-Bang WEI
Acta Physiologica Sinica 2015;67(3):312-318
The plateau pika (Ochotona curzoniae) has a strong adaptability to hypoxic plateau environment. We found that the sperm-specific lactate dehydrogenase (LDH-C4) gene Ldh-c expressed in plateau pika cardiac muscle. In order to shed light on the effect of LDH-C4 on the anaerobic glycolysis in plateau pika cardiac muscle, 20 pikas were randomly divided into the inhibitor group and the control group, and the sample size of each group was 10. The pikas of inhibitor group were injected with 1 mL 1 mol/L N-isopropyl oxamate, a specific LDH-C4 inhibitor, in biceps femoris muscle of hind legs, each leg with 500 μL. The pikas of control group were injected with the same volume of normal saline (0.9% NaCl). The mRNA and protein expression levels of Ldh-c gene in plateau pika cardiac muscle were determined by real-time PCR and Western blot. The activities of LDH, and the contents of lactate (LD) and ATP in cardiac muscle were compared between the inhibitor group and the control group. The results showed that 1) the expression levels of Ldh-c mRNA and protein were 0.47 ± 0.06 and 0.68 ± 0.08, respectively; 2) 30 min after injection of 1 mL 1 mol/L N-isopropyl oxamate in biceps femoris muscle, the concentration of N-isopropyl oxamate in blood was 0.08 mmol/L; 3) in cardiac muscle of the inhibitor group and the control group, the LDH activities were (6.18 ± 0.48) U/mg and (9.08 ± 0.58) U/mg, the contents of LD were (0.21 ± 0.03) mmol/g and (0.26 ± 0.04) mmol/g, and the contents of ATP were (4.40 ± 0.69) nmol/mg and (6.18 ± 0.73) nmol/mg (P < 0.01); 5) the inhibition rates of N-isopropyl oxamate to LDH, LD and ATP were 31.98%, 20.90% and 28.70%, respectively. The results suggest that Ldh-c expresses in cardiac muscle of plateau pika, and the pika cardiac muscle may get at least 28% ATP for its activities by LDH-C4 catalyzed anaerobic glycolysis, which reduces the dependence on oxygen and enhances the adaptation to the hypoxic environments.
Acclimatization
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Animals
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Glycolysis
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Hypoxia
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Isoenzymes
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genetics
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metabolism
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L-Lactate Dehydrogenase
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genetics
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metabolism
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Lactic Acid
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analysis
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Lagomorpha
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genetics
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Male
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Myocardium
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enzymology
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Oxamic Acid
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analogs & derivatives
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Oxygen
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RNA, Messenger
10.Hypoxia at high altitude induces inflammation in the spleen of mice through NOD⁃like receptor signaling pathway
Jiayang Wang ; Ying Hu ; Yuzhen Xu ; Qifu Long ; Chaoqun Tang ; Sheng Yong
Acta Universitatis Medicinalis Anhui 2023;58(9):1498-1506
Objective :
Based on metabolomics and transcriptomics analysis , to explore the molecular mechanism of
spleen inflammation induced by high altitude hypoxia in mice through NOD⁃like receptor signaling pathway .
Methods :
C57BL/6 mice were raised at an altitude of 400 m and 4 200 m respectively , with five mice in each group , and spleen tissues were collected after 30 days . Differential metabolites and differentially expressed genes in key pathways were screened by metabolomics and transcriptome analysis and correlation KEGG enrichment analysis , and the related network interaction diagram of differential metabolites and differentially expressed genes in key pathways was constructed and verified by RT⁃qPCR .
Results :
Metabolomics analysis showed that 133 differential metabolites were screened from in the plain spleen control group (PSC group) and the plateau spleen test group (HST group) , 95 of which were up⁃regulated while 38 of which were down⁃regulated . KEGG enrichment analysis showed that they were mainly involved in NOD⁃like receptor signaling pathway , HIF⁃1 signaling pathway , cholesterol metabolism and other metabolic pathways . The results of transcriptome analysis showed that a total of 4213 differentially expressed genes were identified in PSC group and HST group , including 1947 up⁃regulated genes and 2266 down⁃regulated genes . KEGG was enriched in 173 signaling pathways , including NOD⁃like receptor signaling pathway , MAPK signaling pathway , NF⁃κB signaling pathway and other pathways . Comprehensive analysis showed that the differential metabolites and differentially expressed genes were obviously enriched in NOD⁃like receptor signaling pathway . Therefore , the correlation network interaction map was constructed for the differential metabolites ATP and differentially expressed genes in NOD⁃like receptor signaling pathway . RT⁃qPCR results showed that compared with PSC group , the expression levels of DEGs related to NOD1 and NOD2 (CHUK , TAB3 , MAPK8) in the signaling pathway of NOD⁃like receptor and NLRP1 ⁃CASP1 pathway (NLRP1b , CASP1) in HST group were significantly enhanced . The mRNA expression levels of downstream inflammatory factors IL⁃6 , IL⁃1 β , IL⁃18 , INF⁃γ and TNF⁃α were up⁃regulated and differentially expressed .
Conclusion
Based on the combined analysis of metabolomics and transcriptomics , it was found that hypoxia stimulation at high altitude may affect the NOD⁃like receptor signaling pathway in vivo , and the differential metabolite ATP is positively correlated with the differential key genes in the pathway . ATP mediates the release of downstream inflammatory factors by activating NOD1 , NOD2 pathways and NLRP1 inflammable⁃CASP1 pathways . Inflammatory response occurred in spleen tissue of mice.