BACKGROUND:The metastasis ways of colon cancer include blood metastasis, peritoneal metastasis and lymph node metastasis. Among them, the most common metastasis organ is the liver. It is necessary to establish a simple, suitable animal model to study the biological behavior of the colon cancer liver metastasis, the heterogeneity of cancer cells, and the mechanism of tumor metastasis. OBJECTIVE:To establish animal model of colon cancer liver metastasis with splenectomy injection method. METHODS:Human colon cancer celllines HT29 were cultured, and subcutaneously transplanted tumor was produced in BALB/c mice. Models of colon cancer liver metastases were established through spleen injection and excision of spleen method. The tumor growth and metastasis, the pathology of transplanted tumor, DNA content and cellcycle of the transplanted tumor were al determined. RESULTS AND CONCLUSION:In this study, we successful y established the model of colon cancer liver metastases through the spleen injection via the dorsal midline incision, the tumor formation rate reached 8/8 (100%). Observation of tissue slice showed that the transplanted tumor was adenocarcinoma I-II. Flow cytometry analysis results showed that DNA ploid was heteroploid DNA. This model provides a good technical platform for biology research for colon cancer metastasis.