Objective To investigate the expression and distribution of cyclooxygenase-2(COX-2) in periodontal tissues during experimental tooth movement in rats,and study the relationship between COX-2 and vesscular reconstruction in experimental tooth movement process.Methods Thirty-five healthy Wistar rats were divided randomly into 7 groups on average:normal group and experimental groups for 1,3,5,7,14 and 21 d.A NiTi coil spring with 0.294 N mesial force was connected between first molars of maxillary and the upper incisors.The histological sections were stained with goat anti rat COX-2 antibody,and computer image analysis was used to study the expression of COX-2 in the periodontal tissues of rats.Results Pressure area:compared with normal group(134.75?5.25) the COX-2 expression in 1 d group (147.73?3.27)increased(P0.05).Conclusion The expression of COX-2 in periodontal tissues during experimental tooth movement increase,suggesting that COX-2 can promote the vascular reconstruction in periodontal tissues during orthodontic tooth movement.

Objective To investigate the status of glycemic control in a group of type 2 diabetes mellitus patients combined with pe-ripheral arterial disease before and after operation .Methods Patients with lower extremity arterial occlusive disease who were in charge in vascular surgery of Peking Union Medical College Hospital from 2002 to 2012 were involved .Type2 DM out patient without lower extremity arterial occlusive disease were involved as control .We measured blood pressure ,blood glucose and blood lipid .Fasting,2hPG and average plasma glucose of two groups patients were Compared ,at the same time we observed the difference of plasma glucose before and after operation.The Student′s t test and ANOVA analysis were used to compare the difference of before and after operation and between groups . Results Total of sixty DM patients with lower extremity arterial occlusive disease were involved ,and sixty five patients were involved in control group.There was no difference in age between two groups (65.8 ±8.9 vs 63.0 ±10.0years).The DM patients with lower extremity arterial occlusive disease had longer duration of DM (12.2 ±7.1 vs 7.7 ±5.9years).PAD group had higher fasting plasma glucose (8.3 ± 2.7 vs 7.7 ±2.3mmol/L),post breakfast and lunch plasma glucose (10.7 ±2.9 vs 9.0 ±2.4mmol/L,10.6 ±3.1 vs 8.9 ±4.1mmol/L) than control group .There was no significant difference in post dinner plasma glucose of two groups .Patients with PAD obtained a good plas-ma glucose after they made an operation but insulin Volume had no change ,which was as fasting plasma glucose (7.7 ±2.1 vs 6.8 ± 2.8mmol/L),breakfast (10.5 ±2.5 vs 8.5 ±3.4mmol/L),lunch(10.5 ±3.1 vs 8.7 ±3.8mmol/L)and average plasma glucose (9.6 ± 1.8 vs 8.2 ±2.9mmol/L).Post dinner plasma glucose had no difference in two groups .Conclusion Type 2 DM patients combined with lower extremity arterial occlusive disease had a longer duration of diabetes and higher plasma glucose .At the same,therapy and operation can improve blood glucose control .If a DM patient has a poor plasma glucose ,we should find its reason ,and deal with complication ,then plasma glucose can be controlled better .

Objective To study the electrochemical features of an improved enzyme bioelectrode for biofuel cell. Methods The enzyme electrode was prepared via biotin-avidin technology and its electrochemical features were explored. The conditions such as layers of enzyme films, strength of glucose, scan speed and temperature, which affected the electrochemical behavior of enzyme electrode, were studied. Results The results showed that the electrode had good electric current response to glucose. The enzyme electrode prepared can fulfill the need of biofuel cell and is a promising electrode to be used in human body.

BACKGROUND: Blood vessel remodeling is the basis of the remodeling of orthodontic periodontal tissues. Can He-Ne laser irradiation promote blood vessel remodeling and then accelerate the process of the remodeling of orthodontic periodontal tissues or not?OBJECTIVE: To investigate the effects and action mechanisms of He-Ne laser irradiation on the blood vessel remodeling of orthodontic periodontal tissues of rabbits.DESIGN: Randomized controlled study.SETTING: Animal experimental center of Jilin University.MATERIALS: Thirty-five flap-eared rabbits weighting 2.0 kg were provided by Animal Experimental Center of Jilin University, license No. SYXK(Ji)2002-0002, production license No. SCXK2003-0003. Animal intervention met animal ethical standard. HNGSQ-1 type of He-Ne laser therapeutic instrument was produced in Shanghai. HPIAS-1000 image analytical system was produced by Tongji Medical University. CD34 monoclonal antibody with the product number of MAB-0034 and vascular endothelial growth factor monoclonal antibody with the product number of MS-350 were offered by Fuzhou Maixin Company.METHODS: Experiments were performed at the Provincial Laboratory of Animal Experimental Center of Jilin University from May to December 2003. ①Thirty-five flap-eared rabbits were randomly divided into seven groups, an unforced group and forced 1-, 3-, 5-, 7-, 14- and 21-day groups with 5 rabbits in each group. A stainless steel closing coil spring was deposited between the maxillary first molar and the corresponding maxillary incisor bilaterally, delivering 0.08 N force. The right maxillary of rabbits of each forced group was irradiated by He-Ne laser, and the left side was as control.The cheek corresponding to the right maxillary first molar was selected as irradiation spot and then received He-Ne laser local irradiation. He-Ne laser's wavelength is 632.8 nm, power output of 20.0 mW and energy density of 2.50 J/cm2.Animals of the unforced group did not proceed with anything, sacrificed after raising for a week. Animals of the forced groups were respectively sacrificed on days 1, 3, 5, 7, 14 and 21. ②The premaxillary bones, including the upper first molar, were cut into sagittal sections. Sections were preceded with CD34 and vascular endothelial growth factor immunohistochemical straining. Microvessel density and microvessel area were measured in the tension zone and the pressure zone of orthodontic periodontal tissues. The staining intensity for vascular endothelial growth factor was evaluated by the measurement of gray value using the Computer Image Analyzing System.MAIN OUTCOME MEASURES: ①Gray integral of vascular endothelial growth factor after immunohistochemical staining. ②Microvessel density and microvessel area.RESULTS: Thirty-five rabbits were involved in the result analysis. ①Microvessel density and microvessel area in He-Ne laser irradiated sides were all obviously larger than in the control sides in every forced group in the tension zone and the pressure zone (P ＜ 0.05-0.01), except for the 1-day group. ②In the 1-day group, the vascular endothelial growth factor expressions in periodontal tissues in pressure zone of He-Ne laser irradiated sides were significantly lower than that of control sides (P ＜ 0.05). In the 3- and 5-day groups, vascular endothelial growth factor expressions in pressure zone of irradiated sides were significantly higher than that of control sides (P ＜ 0.05). The vascular endothelial growth factor expressions in pressure zone of both irradiated sides and control sides reached a peak at 5-day group. The vascular endothelial growth factor expressions of irradiated sides were significantly higher than the corresponding control sides in tension zone at 3-, 5- and 7-day group (P ＜ 0.05). The vascular endothelial growth factor expressions in tension zone of both irradiated sides and control sides reached a peak at 14-day group.CONCLUSION: He-Ne laser irradiation promotes blood vessel remodeling of periodontal tissues, increases the vascular endothelial growth factor concentrations in periodontal tissues and makes the angiogenesis of periodontal tissues more active, followed by the remodeling of orthodontic periodontal tissues.

Objective To investigate the level of myocardical injury by measuring serum levels of cTnI, MYO, CK and CK-MB before and after catheter ablation in patients with atrial fibrillation. Methods Forty nine patients underwent catheter ablation for atrial fibrillation were involved in the study, cTnI, MYO, CK and CK-MB levels were measured before and 4 h after catheter ablation. Results Myocardical enzymes were increased in all patients (P0.05). Conclusion The serum myocardial enzymes levels increase after catheter ablation for atrial fibrillation. Of the above myocardical enzymes, cTnI is the most sensitive and specific parameters.

Objective To analyze the prevalence of complications following catheter ablation for atrial fibrillation and to describe the characteristics of transition of complications.Methods From October 2004 to December 2008,2 260 cases(1 265 males)with atrial fibrillation(AF)were admitted for catheter ablation.AF was paroxysmal in 1 449 cases and chronic in 811 cases.Circumferential pulmonary vein isolation(CPVI)was carried out alone for paroxysmal AF and in combination with fractionated electrograms ablation for chronic AF.Complications were summarized and analyzed by dividing the whole duration into three sections:Year 2004-2006,Year 2007 and Year 2008.Results Complications occurred in 61 cases(2.70%).Cardiac tamponade developed in 11 cases,embolism in 18 cases,pulmonary vein stenosis in 14 cases,and vessel access related complications in 18 cases.In Year 2004-2006,cardiac tamponade occurred in 5 cases,cerebral embolism in 3 cases,mesenteric artery embolism in 1 case,pulmonary vein stenosis in 6 cases,and vessel access related complications in 6 cases.In Year 2007,cardiac tamponade occurred in 4 cases,cerebral embolism in 3 cases,Mesenteric artery embolism in 1 case,pulmonary vein stenosis in 4 cases,and vessel access related complications in 5 cases.In Year 2008,cardiac tamponade occurred in 2 cases,cerebral embolism in 7 cases,mesenteric artery embolism in 3 cases,pulmonary vein stenosis in 4 cases,and vessel access related complications in 7 cases.There was no significant difference in the prevalence of complications among three sections.The prevalence of cardiac tamponade was lower in Year 2008 compared with that in the other two sections,P=0.5.However,the prevalence of embolism was higher in Year 2008 compared with that in the other two the sections,P=0.2.Conclusion It is safe to perform catheter ablation for the treatment of AF.Despite the improvement of technical skills,the prevalence of severe complications such as cardial tamponade,pulmonary vein stenosis or stroke did not decrease.

Purpose: To investigate the relationship between the expression of ET, the content of DNA and prognosis in primary lung cancer. Methods: The expressions of ET and the contents of DNA in 66 cancer specimens of different pathological grades were studied by immunohistochemistry and image - analysis technique, all cases were followed up more than 2 years. Results: The positive rate of ET in lung cancer was 73%, which was significantly higher than that in benign bronchial disease (P0.05). The content of DNA in the positive group of ET was significantly higher than that in the negative group (P

Objective To compare the effects of propofol-alfentanil and sevoflurane-N2O anesthesia on cerebral vascular reactivity to the changes in carbon dioxide (CO2). Methods Forty ASA class I - II patients undergoing abdominal operations, were randomly divided into two groups. In group I (n = 20), anesthesia was maintained with infusion of propofol at 0.1mg.kg-1 .min-1, plus alfentanil at 1ug.kg-1. min-1. In group II (n = 20), anesthesia was maintained with 1 % sevoflurane and N2 O-O2 (N2 O: O2 = 3: 2). Mean arterial blood pressure (MAP), heart rate (HR), body temperature (T), end-tidal CO2 tension (PETCO2 ), sevoflurane concentrations, pulse O2 saturation (SpO2 ) and the parameters of the middle cerebral artery blood flow, including mean blood flow velocity (Vm), diastolic blood flow (Vd), pulse index (PI) and resistance index (RI), were measured at following occasions: after intubation and when PETCO2 reached 40, 45, 50, 45, 40, 35, 30 and 40mmHg, which was achieved through adjusting mechanical ventilation. The CO2 reactivity index (CRI) was calculated as delta Vm/delta PETCO2 ratio. Results CRI and Vm were lower in propofol--alfentanil group than sevoflurane-N2O group (P

Objective To Investigate the expression of metallothionein (MT) and p53 in non-small cell lung carcinoma(NSCLC). Methods The expression of MT and p53 was detected by immunohistochemical method in the paraffin-embedded specimens of 73 NSCLCs and 15 normal bronchial mucosal tissues(NBMT). Results [WTBZ]The positive rates of MT and p53 expression in NSCLC were 54.8%(40/73)and 65.8%(48/73), respectively, and in NBMT were 20.0%(3/15)and 33.3%(5/15), respectively. The positive rates of both MT and p53 in NSCLC were significantly higher than those in NBMT(P0.05). The positive rates of MT and p53 were closely correlated with the histological grades and lymph node metastasis of NSCLC(P

Objectives To determine the changes of right ventricular hemodynamic in the early phase after lung resection and non-lung resection thoracotomy by Doppler echocardiography and to investigate the correlation with preoperative lung function.Methods 55 patients who underwent thoracotomy were divided into two groups. In group A: 15 patients underwent non-lung resection thoracotomy; in group B: 40 patients underwent lung resection. The group B were subdivided into group B1 (n=23) and group B2 (n=17), acording to the preoperative lung function results: Artery blood gas analysis were measured and right ventricular hemodynamic indices were calculated by Doppler echocardiography before and after operation,respectively.Results Right ventricular ejection fraction (RVEF) and artery oxygen pressure(PaO 2) significantly decreased (P