Objective To study the effect of volatile chemicals in the air of newly decorated rooms on O6-methylguanine-DNA methyltransferase (MGMT) expression in the lung, liver, kidney and testicle tissues of mice. Methods Forty Kunming mice were randomly divided into four groups and the imitate pollutants mixture of volatile chemicals in the air of newly decorated rooms was used as the exposed toxicants: low-dose exposure group (7.5 mg/m3), moderate-dose exposure group (15 mg/m3), high-dose exposure group (30 mg/m3), the control group (no exposure ). The mice were exposed 4 hours each day, for nine weeks. Streptavidin and biotechnology-peroxidase (SABC) immunohistochemical method was used to determine the MGMT expression in the lung, liver, testicle and kidney tissues. Results The expression of MGMT in the liver, lung, testicle tissues in the high, moderate and low-dose groups reduced significantly compared with the control group (P0.05). Conclusion The higher exposure of volatile chemicals from the air of newly decorated rooms may inhibit the MGMT expression in the liver, lung and testicle tissues of mice.

Purpose: To investigate the relationship between the expression of ET, the content of DNA and prognosis in primary lung cancer. Methods: The expressions of ET and the contents of DNA in 66 cancer specimens of different pathological grades were studied by immunohistochemistry and image - analysis technique, all cases were followed up more than 2 years. Results: The positive rate of ET in lung cancer was 73%, which was significantly higher than that in benign bronchial disease (P0.05). The content of DNA in the positive group of ET was significantly higher than that in the negative group (P

Objective To assess the epidemiological relatedness on an outbreak of Enterococcus facium sepsis between humans and pigs based on genomic analysis. Methods Two bacterial isolates recovered randomly from the blood of one patient and one pig were analyzed for homogeneities by comparison with 16S rRNA gene sequences in the GeneBank. Moreover, The extracted genomic DNA was digested with the 20 U SamI enzyme respectively, then their interrelationship was performed according to the pulsed field gel electrophoresis. Results Sequences determined from both human and pig isolates were 100% identical and most closely related to E. facium , diverging from the prototype sequence by one nucleotide (99.9% similarity) and displayed indistinguishable pulsed field gel electrophoresis patterns. Conclusions These data demonstrate epidemiological relatedness of the bacterial isolates, and suggest spread of an E. facium -related sepsis outbreak from pigs to humans.

ObjectiveTo observe the therapeutic effect and recurrence of treating Benign Paroxysmal Positional Vertigo(BPPV) by the treatment that combined Canalith Repositioning Maneuver(CRM) with Chinese and Western medicine.MethodsCollecting 40 cases suffered from BPPV,and randomized controlled method was used.Control group:20 cases( using Western and Chinese medicine),treatment group:20cases( using Western and Chinese medicine wrbined with CRM),judge the effect after 7days and telephone followed up 3 months,inquiring about recurrence situation.ResultsCure rate of treatment group was 90％,total effective rate was 100％ which was higher than the control group which was 50％,the total effective rate was 95％ (P ＜0.001 ) ;20 cases in treatment group followed up by telephone didn't recur.1 case in control group recurred,but got better after the treatment of CRM.ConclusionCRM which was effective,safe,simple and convenient to treat BPPV could be used as the preferred treatment for BPPV,if combined with Chinese and western medicine,it could significantly alleviate the symptoms and reduce the relapse.

Objective To investigate the effects of resveratrol on epileptic seizure and cognitive impairment and the role of CREB in rats with temporal lobe epilepsy (TLE). Methods TLE rat model was established by LiCl?pilocarpine injection and resveratrol was administrated at a dose of 15 mg/(kg · d) for 7 days. The rat behavior was evaluated by Racine scale and the CREB expression and phosphorylation in temporal cortex were detected with western blot. The Morris water maze was used to assess the learning and memory. Results Resveratrol administra?tion significantly reduced the Racine scales of TLE rats. The CREB phosphorylation in TLE group was increased compared with that in control group, which was remarkably inhibited by resveratrol. The escape latency of TLE group was increased at 4th, 5th and 6th day after training, and time in target quadrant was reduced compared with that in control group. Resveratrol significantly decreased latency time and increased target quadrant time of TLE rats. Conclusion In TLE rats, resveratrol administration suppresses epileptic seizure and improves cognitive function, which might be mediated by inhibition of CREB phosphorylation.

Objective To evaluate the effect of mining waste water on drinking water quality and tumor mortality of villagers. Methods Analyse quality of drinking water sampled from well which only supply water to a family which the well belongs to and survey the death reasons of whole population in the polluted area and the control area. Results Mining waste water discharged by a mine contaminated the nearby river and impacted the drinking water quality of the nearby village also. Compared with the control, the concentration of lead, arsenic and mercury in drinking water of polluted area was much higher. The 5,year total mortality in polluted area and in the control was 3.09% and 1.55% respectively. Tumor mortality in polluted area was 50%, esophagus cancer mortality was 54.24%. Tumor mortality in the control area was 20% only and most tumors were liver cancer. Conclusion The adverse effect of mining waste water on drinking water quality and human health in polluted area has been seen.

Objective To evaluate influence of patient-controlled intravenous analgesia (PCIA) and patient-controlled epidural analgesia (PCEA) on expressions of serum inflammatory cytokines in elderly patients with hemi or whole hip replacement using cemented artificial joint. Methods Elderly patients undergoing hip replacement were selected and were divided into PCIA group and PCEA group. VAS scores were calculated at 12 h postoperatively. Patients whose VAS scores were not more than 2 at postoperative 12h were included. 30 cases in each group were finally included. Fifteen cases were randomly chosen in each group and underwent sample blood drawing for assays. Expressions of serum inflammatory cytokines were detected by ELISA , RT-PCR and Western-blot. Results Gene and protein expressions of TNF-a as well as IL-6 in group PCEA were lower and expression of IL-10 was higher than that in group PCIA. Serum level of TGb-β was higher in group PCEA detected by ELISA. There was no significant difference in expression of IL-8 between groups. Conclusions PCEA may better promote expressions of anti-inflammatory cytokines and inhibit expressions of proinflammatory cytokines. PCEA is superior in maintenance of inflammatory cytokine balance.

AIM:To explore the relationship and molecular mechanism between microRNA-21(miR-21) and Schwann cells ( SC) following peripheral nerve injury.METHODS: The mRNA expression of miR-21 and phosphatase and tensin homologue deleted on chromosome ten ( PTEN) in animal model were detected by real-time PCR.The over-ex-pression of miR-21 and inhibition of miR-21 expression in the Schwann cells according to transfection of lentiviral vectors were performed, the nonspecific miRNA was used as a negative control ( NC) .The cell apoptosis was measured by flow cy-tometry.The mRNA expression of miR-21 and PTEN in the cells was detected by real-time PCR.The protein expression of PTEN and cleaved caspase-3 was determined by Western blot.RESULTS: The level of miR-21 was significantly higher and the mRNA level of PTEN was significantly lower in the model of nerve injury than those in control group.miR-21 over-expression decreased the number of apoptotic Schwann cells compared with NC-SC.The mRNA expression of PTEN was down-regulated by over-expression of miR-21.The protein expression of PTEN and cleaved caspase-3 was down-regulated by over-expression of miR-21 (P<0.05).CONCLUSION: miR-21 may play an important role in the peripheral nerve injury through inhibiting apoptosis of Schwann cells by down-regulating the expression of PTEN.

AIM: To investigate the molecular mechanism of microRNA-21 (miR-21) in the regulation of Schwann cell proliferation following nerve injury.METHODS:The expression of miR-2l was detected by real-time PCR. Synthetic miR-21 mimic and its control were transfected into rat Schwann cells.CCK-8 assay was performed to evaluate the influence of miR-21 on the proliferation of Schwann cells.The cell cycle distribution was determined by flow cytometry. The expression of transforming growth factorβ-induced protein ( TGFBI) and cyclin D1 were detected by Western blotting. RESULTS:The expression of miR-21 in model group was 7.87 ±0.75 and 7.75 ±0.80 times higher than that in sham operation group and blank group respectively.After transfected with miR-21 mimic, the expression of miR-21 in experimen-tal group was 2.21 ±0.14 and 2.29 ±0.21 times higher than that in negative control group and blank group respectively. Moreover, the A450 value of CCK-8 assay in experimental group at 48 h was higher than that in negative control group and blank group.The proliferation index in experimental group was higher than that in negative control group and blank group. At the same time, the expression of TGFBI obviously decreased and the cyclin D1 increased in the Schwann cells 48 h after transfection with miR-21.CONCLUSION:miR-21 promotes the proliferation activity of Schwann cells by down-regulating TGFBI expression.

Aim To study the inhibitory effects of ex-tractive pericarpium trichosanthes ( EPT) on high glu-cose-induced apoptosis in human umbilical vein endo-thelial cells ( HUVECs ) and its underlining mecha-nisms. Methods HUVECs were cultured. Effects of EPT at different concentrations on the high-glucose-in-duced apoptosis in HUVECs were observed. The cell viability of HUVECs was determined by MTT colori-metric method. Cell apoptosis was identified by Ho-echst staining. The intracellular activity of Caspase-3 was detected with colorimetry. Protein expression and p65 nuclear translocation of NF-κB were detected by Western blot and immunofluorescence staining. Re-sults Treated with 30 mmol · L-1 glucose for 48 hours had a significantly decrease on cell viability com-pared with control, and the apoptotic rate and Caspase-3 activity were increased markedly, the protein expres-sion of NF-κB was upregulated and p65 nuclear trans-location in HUVECs increased; Pre-incubation with EPT(12. 5,25,50 mg·L-1 ) for 1 hour enhanced the cell viability, and decreased the apoptotic rate and Caspase-3 activity and downregulated the expression of NF-κB in a dose-dependent manner. Moreover, EPT could inhibit p65 translocation. Conclusion EPT can protect HUVECs against the apoptosis induced by high glucose in vitro,and its mechanism may be related with downregulation of NF-κB expression and inhibition of the intracellular activity of Caspase-3 .