Objective To observe the clinical efficacy of XIE’s Wen Du Qu Mai (Warming the Governor Vessel to expel smog) needling method plus heat-sensitive moxibustion in treating chronic simple rhinitis due to yang deficiency. Method Eighty patients with chronic simple rhinitis due to yang deficiency were randomized into a treatment group and a control group, 40 in each group. The treatment group was intervened by acupuncture plus heat-sensitive moxibustion, while the control group was by medication. The symptom and sign score was observed before and after 1-week treatment, and the clinical efficacy was compared. Result The total effective rate was 85.0%in the treatment group versus 55.0% in the control group, and the difference was statistically significant (P<0.05). The symptom and sign scores were changed significantly after treatment in both groups (P<0.01,P<0.05). After treatment, the symptom and sign score in the treatment group was significantly different from that in the control group (P<0.05). Conclusion XIE’s Wen Du Qu Mai needling method is effective in treating chronic simple rhinitis due to yang deficiency.

Objective To evaluate the size of the liver tissue necrosis at different power and energy, to search the size of liver tissue necrosis at ideal power and energy, and to provide dependable data for semiconductor laser-therapy in malignant hepatic tumor. Methods Ten rabbits were included, and the rabbit liver tissue was fired using semiconductor laser at 1 W, 2 W, and 3 W with the energy of 200 J, 400 J, 600 J, 800 J, and 1 000 J. The diameters of the necrosis and charring in the target area were measured. Results The diameter of the liver tissue necrosis and charring was from 4.3 mm and 7.8 mm at 1 W to 10.4 mm and 21.9 mm at 2 W in 1 000 J. From 2 W to 3 W, the diameter of the liver tissue necrosis and charring remained unchanged. At the power of 2 W, the diameter of liver tissue necrosis and charring was proportional to the energy, and the areas of necrosis were correlated with that of charring. Conclusion Semiconductor laser photocoagulation is safe and feasible. This experiment supplies valuable laboratorial data for further clinical study.

Objective:To investigate the change and related factors of nitric oxide,nitric oxide synthase level and cerebral blood flow(CBF) in panic disorder.Methods:30 patients with panic disorder,30 patients with generalized anxiety disorder and 22 normal controls entered the study.Serum level of NO and NOS were assayed.Cerebral blood flow were measured with TCD.Results:The concentration of NO was significantly lower in panic disorder group in comparison with GAD group.There was no significant difference in NOS level between panic disorder group and the control group.Cerebral blood peak flow velocity in the left and right middle cerebral artery and mean cerebral blood flow velocity in the right middle cerebral artery were lower than normal control group and the difference were very significant.Cerebral blood peak flow velocity in the right vertebral artery was lower than those of GAD and NC group.The concentration of NO in panic disorder was correlated with HAMA score negatively.Cerebral blood peak flow velocity in the left middle cerebral artery was correlated with mean Cerebral blood flow velocity in the left middle cerebral artery,peak CBF velocity in the right middle cerebral artery and psychological anxiety positively and correlated to cerebral blood peak mean velocity in the right middle cerebral artery,peak CBF velocity in the right anterior cerebral artery,age and female negatively.Cerebral blood peak flow velocity in the right middle cerebral artery has positive correlation with mean cerebral blood flow velocity in the right middle cerebral artery and peak CBF locity in the right anterior cerebral artery and negative correlation with cerebral blood mean flow velocity in the right anterior cerebral artery. Mean cerebral blood flow velocity in the right middle cerebral artery was correlated to cerebral blood peak flow velocity in the right middle cerebral artery,mean cerebral blood flow velocity in the left middle cerebral artery,cerebral blood mean flow velocity in the right anterior cerebral artery and NO level positively and age,cerebral blood peak flow velocity in the left middle cerebral artery and cerebral blood peak flow velocity in the right anterior cerebral artery negatively.Cerebral blood peak flow velocity in the right vertebra artery has positive correlation with cerebral blood mean flow velocity in the right vertebra artery and cerebral blood mean flow velocity in the left middle cercbral artery and negative correlation with cerebral blood peak flow velocity in the left anterior cerebral artery and cerebral blood mean flow velocity in the right anterior cerebral artery.Conclusion:The change of NO and cerebral blood flow may be one of the neurobiological mechanisms in panic disorder.To assay the level of NO and measure cerebral blood flow might become approach of diagnosis for panic disorder.

Objective To explore the surgical skill, safety, and cosmetic outcomes of complete endoscopic subcutaneous mastectomy and immediate reconstruction with implants for breast cancer. Methods From August 2004 to September 2007, 31 women with breast cancer received complete endoscopic subcutaneous mastectomy and immediate reconstruction with implants in our hospital after neoadjuvant chemotherapy. The operation spaces were established by insufflating carbon dioxide after lipolysis and liposuction in the subcutaneous stratum and retromammary space. Subcutaneous mastectomy was carried out under a complete endoscope, sentinel lymph node biopsy or axillary lymph node dissection, and immediate reconstruction with implants were carried out via axillary small incision. Results The operations were completed in all of the 31 cases. Sentinel lymph node biopsy was performed on 15 cases (including supplemental axillary lymph node dissection in 8), and immediate axillary lymph node dissection was done in 16 cases. No cancer infiltration on tumor surface or into the nipple basement was observed in frozen sections during the operation. In one patient, the nipple was partly necrotic. No other complications were found in this series. Three months after the operation, cosmetic results were good in 22 cases (71.0%), average in 7 (22.6%), and poor in 2 (6.4%). The patients were followed for 3 months to 3 years (over 1 year in 15 patients), during which no one had metastasis or recurrence of the tumor. Conclusions Complete endoscopic subcutaneous mastectomy combined with immediate reconstruction with implants is less traumatic and safe with few complications and good cosmetic result. The method is a good choice for patients with early-stage breast cancer.

Objective To evaluate the value of DSA in the diagnosis of staging moyamoya disease (MMD).Methods DSA of 31 patients with MMD were reviewed retrospectively. Results The stenosis or occlusion with different extents of the supracranial internal carotid arteries and the collateral circulations were shown in DSA of the all patients. MMD lesions of 57 cerebral hemispheres shown in the DSA of 31 patients were diagnosed by staging according to the description of Suzuki: Stage Ⅰ in 4 cerebral hemispheres; Ⅱ,10;Ⅲ17;Ⅳ12;Ⅴ6 and Ⅵ,8. Conclusions DSA could show clearly the stenotic degree, occlusive site and collateral circulation of the MMD and simultaneously with the staging of the disease; and thus guiding the choice for early surgical teatment.

Objective:To reveal the action of psoriatic peripheral blood T lymphocytes on keratinocytes proliferation and the significance in the pathogenesis of psoriasis.Methods:Keratinocytes were cocultivated with psoriatic peripheral blood T lymphocytes in comparison with those cocultivated with normal T lymphocytes. Immunohistochemical technique was performed to detect the expression of Ki67, c-Myc and Bcl-xL proteins.Results:There were significant overexpression of Ki67, c-Myc and Bcl-xL proteins in keratinocytes cocultivated with psoriatic T lymphocytes compared with the uncocultivated group and normal T lymphocytes group. Expression of Ki67, c-Myc and Bcl-xL proteins in keratinocytes cocultivated with normal T lymphocytes were not significantly different from the uncocultivated group.Conclusion:Psoriatic T lymphocytes, which have specific activity, can induce keratinocytes abnormal pattern of proliferation. One of the important mechanisms might be its action on the expression of c-Myc and Bcl-xL proteins.

The age of 18 is an important criterion in judicial trial, immigrant and competitive sports. Consequentially, the estimation of age 18 is a key issue in forensic practice and research. The extremitas sternalis claviculae, iliac crest, third molar, and the proximal limb of the limb bone were usually used as indictors of age 18. The results of the previous studies demonstrated that those indictors could be beneficial to the estimation of age 18. The X-ray, CT, ultrasound and MRI of different indictors were widely utilized for the estimation of age 18, particularly the thin-layer CT. But due to the non-radiation, MRI will be a trend for forensic age estimation in the future. Whilst in the previous studies, the descriptive analysis was applied for the estimation of age 18, but due to the low statistic efficiency, it is unsuitable for forensic age estimation, and the future studies should pay attention to the high efficiency statistical methods, for instance, the ROC curve or the data mining.

Objective:To explore the proliferation inhibition and radiosensitization of Biyanqing granule on nasopharyngeal carci -noma cell line CNE-2 in vitro.Methods:CNE-2 cells were cultured in vitro.The inhibition of Biyanqing granule on the proliferation of CNE-2 cell was evaluated by MTT assay .Radiosensitization was explored by clone formation assay , and cell cycle and apopotosis were observed by flow cytometry ( FCM) .Results:Biyanqing granule could inhibit the proliferation of CNE-2 in a time-and dose-dependent manner.The IC50 in 24, 48 and 72 h was 70.79, 60.13 and 51.63 mg· ml-1(calculated according to the weight of all medicinal ma-terial), respectively.The colony formation assay showed that Biyanqing granule combined with radiation could significantly reduce the colony formation of CNE-2 cells.With the concentration increase of the main drug , the colony formation of CNE-2 cells was reduced . The number of colony formation in the negative control group , the radiation group , 10 mg· ml-1 and 20 mg· ml-1 Biyanqing combined with radiation groups (calculated according to the weight of all medicinal material ) was significant different (P<0.05).With the main drug concentration increasing , the percentage of G 2/M phase and apoptotic cells were both increased , and compared with the con-trol group, the difference was significant (P<0.05).Conclusion:Biyanqing granule can not only inhibit CNE-2 cells but also block CNE-2 cells in G2/M to improve the radiosensitization of CNE-2 cells.

BACKGROUND: Ghildren refractory clubfoot includes idiopathic multiple contracture, untreated idiopathic clubfoot in older children and failure cases of repeated operations. It is very difficult to treat this type of children patients. It has important significance in the exploration of new therapeutic methods.OBJECTIVE: To improve the therapeutic effectiveness of refractory clubfoot in children with Ilizarov method.DESIGN: a self-controlled study.SETTING: Department of Orthopedics, Nanjing Children' s Hospital, Nanjing Medical University.PARTICIPANTS: Totally 3 cases of children idiopathic multiple contracture, 2 cases of idiopathic clubfoot in children aged more than 10 years old without treatment and 6 cases of postoperative recurrent idiopathic clubfoot were admitted by the Department of Orthopedics of Nanjing Children' s Hospital Affiliated to Nanjing Medical University during 1999 to 2000. All cases manifested abnormal ankylosis and shortening deformity in foot.METHODS: Twelve refractory clubfeet in eleven patients were treated with the Ilizarov method. After achilles tendon lengthening and posterior release,kieschner' wires or olive wires with tension were penetrated through multiple planes of the middle lower 1/3 of tibia, calcaneus and the 1st to the 5th metatarsals, which were fixed respectively to external bone fixer of a ring,half-ring or horseshoe shape and connected with each other by multiple screw bars into a three-dimensional external bone fixing set. Plantarflexion, inversion, adduction and shortening deformities were corrected by the adjustment of the distance between each part of the external bone fixing set through extension, compression, and rotation, etc., and navicular and cuboid osteotomy.MAIN OUTCOME MEASURES: All 11 cases were followed up for 2 to 10 years with an average period of 4. 5 years. Indices like foot morphology,talocrural articualr activity range and deformity correction were observed during the follow up.RESULTS: The results were excellent in 5 cases (6 feet), good in 4 cases (4 feet), fair 2 cases(2 feet) according to the Garceau standard scale. The rate of excellent and good clinical results was 83.3%. Mild forefoot adduction was left in 2 feet of 2 cases and quite obvious tarsal articular ankylosis was found in 4 feet of 4 cases; however, there were no significant differences of gait and load bearing between these patients and normal children.CONCLUSION: The Ilizarov technique is a safe and effective method for the treatment of refractory children clubfoot while its indications should be controlled strictly.