ObjectiveTo observe the therapeutic effect and recurrence of treating Benign Paroxysmal Positional Vertigo(BPPV) by the treatment that combined Canalith Repositioning Maneuver(CRM) with Chinese and Western medicine.MethodsCollecting 40 cases suffered from BPPV,and randomized controlled method was used.Control group:20 cases( using Western and Chinese medicine),treatment group:20cases( using Western and Chinese medicine wrbined with CRM),judge the effect after 7days and telephone followed up 3 months,inquiring about recurrence situation.ResultsCure rate of treatment group was 90％,total effective rate was 100％ which was higher than the control group which was 50％,the total effective rate was 95％ (P ＜0.001 ) ;20 cases in treatment group followed up by telephone didn't recur.1 case in control group recurred,but got better after the treatment of CRM.ConclusionCRM which was effective,safe,simple and convenient to treat BPPV could be used as the preferred treatment for BPPV,if combined with Chinese and western medicine,it could significantly alleviate the symptoms and reduce the relapse.

Objective To investigate whether hepatocyte growth factor ( HGF ) and insulin-like growth factor (IGF1) induce cardiac stem cells (CSCs) to proliferate and directly differentiate into cardiomyocytes in vitro.Methods The myocardial tissues were dissected for primary culture of CSCs with the method of explants .The expressions of c-kit and CD34 were examined with immunofluorescence .Primary cells were purified with c-kit by flow cytometry.CFDA SE fluorescent probe was used to detect the proliferation of c-kit+CSCs.C-kit +CSCs were divided into two groups , and cardiac stem cells group and co-cultured with cardiomyocytes group , both group were cultured with HGF and IGF 1.An inverted microscope was used to observe changes in cell number and morphology in different periods .Living cells workstation was used to observe CFDA SE fluorescence intensity , to acquire images and do statistical analysis .Immunofluorescence technique was used to detect the expression of Nkx 2.5 and cardiac troponin T .Results In cardiac stem cells group ,CSCs had no obvious changes in cell number .In co-cultured with cardiomyocytes group , CSCs proliferated and had changes in morphology .Nkx2.5 and cTnT were positively expressed . Several CSCs differentiated into beating cardiomyocytes . Conclusion In co-cultured with cardiomyocytes condition , HGF and IGF1 may promote CSCs to proliferate and differentiate into beating cardiomyocytes .

The relationships of the quality and quantity of dietary protein, the level of fasting serum and 8-hour overnight urine free amino acids and blood pressure were studied in three natural Chinese populations. The results showed that the ratio of dietary animal protein/total dietary protein, the ratio of dietary protein/dietary sodium, serum and urine taurine, lysine etc were inversely associated with blood pressure significantly. Alanine was positively associated with blood pressure. Most serum free amino acids were positively correlated with the ratio of dietary protein/dietary sodium. Urine taurine, lysine and asparagine were positively correlated with dietary protein, dietary and urine sodium significantly. These results suggested that high quality protein which is rich in taurine, lysine etc might prevent hypertension.

Objective To explore the effect of kangfuxin liquid topical treatment on open wound of postoperative perianal abscess.Methods200 cases of patients with open wound abscess surgery in our hospital from February 2014 to September 2016 were randomly divided into control group and experimental group,with 100 cases in each group.The control group was given conventional treatment, the experimental group were treated by kangfuxin liquid.The relevant therapeutic indicators and clinical healing time between two groups were compared.ResultsThe effective rate of the treatment group was 93.0%, which was significantly higher than that of the control group (85.0%), the difference was statistically significant(P<0.05).The granulation growth period of the experimental group was (5.14±1.41) days, and the average healing time was (15.21±2.49) days.Which were significantly shorter than those of the control group[(7.45±1.32) days, (21.16±2.69) days], and the differences were Statistical differences (P<0.05).ConclusionTo improve the treatment effect to a great extent open wound infection of kangfuxin liquid in treatment of perianal abscess after surgery, shorten the healing time, improve the patient's symptoms, with further clinical promotion and application significance.

Objective To investigate cell type and size of fetus atrioventricular node. Methods Using the technique of paraffin section, H-E staining, the cellular morphology and size of atrioventricular node in fetus hearts were observed under microscope, and quantitied by HIPAS-1000 computerized image analytical instrument. Results The cell types of atrioventricular node in fetue hearts were composed of light, dark and transitional cells. The morphology of light and dark cells were spindle-shaped or ellipsoid, but a few cells were polygonal. The shape of transitional cells was short column. The nuclei of light cells and transitional cell were larger than that of dark cells.Conclusion Pacemaker cell of atrioventricular node in fetus heart includes light and dark cells which could present different function types of pacemaker cell. [

BACKGROUND: Neuropeptide Y (NPY) neurons are extensively located in various brain regions such as cerebral cortex, caudate-putamen nucleus, syslimbic system, thalamus and brain stem. They are also involved in various brain activities such as motor learning, motor memory and motor integration. Considering the fact that cerebellum can reorganize through motor learning, we tried to identify the morphology and distribution of NPY neurons in rat's cerebellar cortex to obtain the morphologic knowledge that is related to its cerebellar-cortex-based motor learning.OBJECTIVE: To investigate the morphology and distribution of NPY -immunoreactive neurons in rat's cerebellar cortex, and discuss the relationship between NPY neurons and cerebellum motor learning and motor memory.DESIGN: A single-sample-study based on animal samples.SETTING: Anatomy Department, Pathophysiology Department and Morphology Center in Xinxiang Medical College.MATERIALS: From July to December 2001, the experiment was performed at the Morphology Center in Xinxiang Medical College. Ten Sprague-Dawley (SD) rats, clean grade, regardless of their gender and weighing 100-200 g,were selected.METHODS: After intraperitoneal injection anesthesia and ascending aorta infusion fixation, the cerebellum was taken out by craniosurgery. The cerebellum was immersed in the same fixative fluid for duration of 48 hours, and then was embedded in paraffin. The next step was to make continuous sagittal sections. NPY neurons were identified by SP immunohistochemical staining, using rats cerebral section as the positive control. In the negative control, the first antibody replaced by Bovine Calf Serum(BCS), and the second antibody replaced by 0.01 mol/L PBS. Sequentially the light-microscopic observation and micrography were recorded.MAIN OUTCOME MEASUREMENTS: The Morphology and distribution of NPY neurons in rat's cerebellar cortex were taken as main outcome measurements.RESULTS: NPY-immunoreactive neurons were distributed in the Purkinje layer of the cerebellum. They had pear-like or spherical shapes, clear and unstained nucleus, unstained axons and dendrites, and their axons stretching out into the granular layer while the reticular dendrites elongated into the molecular layer. NPY-immunoreactive fibers, instead of neurons, were found in the molecular layer and granular layer.CONCLUSION: NPY-immunoreactive neurons in the Purkinje layer of the cerebellum may be involved in motor learning, motor memory, visceral activity and higher motor integration.

Objective To summarize the clinical experiences in partial splenectomy for the treatment of splenic injuries Methods The indications, surgical procedures and surgical outcomes of a total of 105 cases undergoing partial splenectomy for splenic injuries in Southwest Hospital in the past 16 years were analyzed retrospectively Results Sixty seven cases(63 8%) suffered from isolated splenic injury According to splenic injury scale, grade Ⅰ (13, 11 4%), grade Ⅱ (56, 53 3%), gradeⅢ (28, 26 7%) and grade Ⅳ(8, 7 6%) were found in 105 cases of splenic injuries Surgical procedures included segmental splenectomy in 41 cases, hemi splenectomy in 35 cases, subtotal splenectomy in 29 cases and autotransplantation of splenic tissue in 21 cases of splenic injury in children There was no in hospital death in all cases Severe complications of subdiaphragmatic fluid collection and infection occurred in 3 cases, but the patients were cured by conservative treatment Conclusion Partial splenectomy is a safe and reasonable choice for selected patients with splenic injury

Objective: To assess the technique, efficacy, security and side effects of percutaneous ethanol injection(PEI) in treating the cystic thyroid adenomas. Methods: Fifty seven patients(male 34, female 23) with unilateral and solitary benign cystic thyroid adenoma received PEI therapy under high resolution ultrasound guidance. The medial age of the patients was 47 years(ranging 29 62). The mean diameter in the largest section of the thyroid adenomas was (37.55?5.64) mm(17 59 mm). The thyroid lesions in 12 patients consisted of cystic lumen mixed with solid structure, and the rest 45 had entire cystic thyroid lesions. Ultrasound reexamination of thyroid lesions was done 3 months after the initial treatment. Significant curative effect was defined as an adenoma volume shrinkage rate larger than 50%. The efficacious result was defined as a volume shrinkage rate between 40% and 50% together with a further shrink over a 6 month duration follow up. Those patients with adenoma volume shrinkage rate less than 40% underwent a second PEI after 6 months. Results: Over a mean duration of 25.5 months follow up, 66.7% subjects with entirely cystic had significant curative outcome; 19.3% subjects had efficacious result; and 9.8% subjects needed a second PEI and their thyroid lesions contained more solid tissue. There were 5.3% subjects were transfered to open surgery due to colloid cyst fluid. Twenty one patients complained of intraoperative local transient pain; 15 patients had transient pyrexia in the 48 h after injection, and 3 patients were affected by post operative transient dysphonia. No serious bleeding were encountered, and no apparent change of serum T 3,T 4,TSH level was observed. Conclusion: Ultrasound guided PEI therapy is an effective, safe and simple procedure for the ablation of cystic thyroid adenoma. Careful selection of subjects according to their preoperative thyroid ultrasonographies can reduce the unsuccessful PEI. A selective volume of ethanol injection consistent with the adenoma size on sonograms and a thorough flush with ethanol can improve the efficacy of PEI.

Objective:To observe the effect of diammonium glycyrrhizinate injection on bronchus,lung tissue and amount of eosinophils(EOS)in rats with asthma and to discuss the mechanism of diammonium glycyrrhizinate intervention on airway inflammation in asthma.Methods:To divide 60 male SD rats into 6 groups as following:normal control group,model group,dexameth group,high dose of diammonium glycyrrhizinate group,middle dose of diammonium glycyrrhizinate group and low dose of diammonium glycyrrhizinate group(n=10).After setting up the model of rats with asthma,we detect the EOS in both rats'blood serum and bronchoalveolar lavage and observe the pathological change in bronchus and lung tissue in rats of each group.Results:Not only the amount of EOS of rats in model group is bigger than that in normal control group(P

OBJECTIVE:To prepare Luhuifangba burn cream and establish a method for its quality control.METHODS:The burn cream was prepared from freeze-dried powder of aloe barbadensis miller gel,borneol,hexadecanol,cosmolin,sodium dodecylsulfate,glycerol,ethylparaben and distilled water.Polysaccharide in the preparation was identified by paper chromatography,and borneol was identified by means of the color reaction between1%vanillin-sulfuric acid and bornyl alcohol and isoborneol.The content of polysaccharide in the cream was determined using anthrone-sulfuric acid method.RESULTS:The preparation was a cream in ivory white color,which spread easily and smelled slightly oily.Polysaccharide and borneol in aloe were able to be identified by the above-mentioned method.The linear range of polysaccharide was0.02～0.2mg/ml,and the average recovery was106.3%(RSD=2.5%).CONCLUSION:The preparation method is feasible,and the quality control method is simple,accurate,and reproducible.