Objective To investigate the effect of calcium acetate in treatment of elderly patients with CAPD in peritoneal dialysis patients with hyperphosphatemia and efficacy.MethodsFrom March 2013 to January 2016,46 elderly patients with CAPD were randomly divided into experimental group and control group.The control group were used calcium 1.5mmol/L Liquor Dialysisintraperitoneus CAPD treatment,and control diet.Besides these treatments,the experimental group were given calcium acetate tablets 668mg/3 times every day after a meal, 2 tablets each time.All patients were detected before treatment and 4,6 and 10 weeks after the serum calcium, phosphorus and calcium and phosphorus in blood iPTH index, calculate the product of calcium and phosphorus,record and statistical analysis.ResultsTwo groups after 4 week of treatment, all outcome measures were decreased;the experimental group blood phosphorus decreased significantly after 6 weeks treatment;it after 10 weeks of treatment, serum calcium(t=5.202),phosphorus(t=7.767),blood iPTH(t=-10.324) and the calcium phosphorus product (t=-4.106) compared with that before treatment,there were statistically significant differences(P<0.01);the control group after 10 weeks of treatment, with all outcome measures there were no significant differences.The experimental group after 10 weeks of treatment,serum calcium(t=-4.055),phosphorus (t=-9.037),blood iPTH(t=9.940) and the calcium phosphorus product(t=-4.211)compared with the control group,the differences were statistically significant(P<0.01).ConclusionCalcium acetate treatment can significantly reduce the blood serum of aged CAPD in peritoneal dialysis patients with high phosphorus,effectively prolong the survival time and improve the quality of life during treatment.

Objective To investigate chemical constituents in the leaves of Euphorbia soongarica(Xinjiang origin).Methods The leaves were extracted with 95% EtOH and partitioned with petroleum ether,CHCl3,and n-BuOH,respectively.Compounds were isolated by using repeated silical gel column chromatography and Sephadex LH-20 column chromatography.Results Eight compounds were identified on the basis of physiochemical properties and spectral data as chrysoeriol(Ⅰ),phthalic acid,bis-(2,5-dimethylhexyl) ester(Ⅱ),fraxetin(Ⅲ),3-(4-O-?-D-glucopyranosyloxy-3,5-dimethoxy)-phenyl-2E-propenol(Ⅳ),fraxin(Ⅴ),3-methylellagic acid-3′-O-?-D-xylopyranoside(Ⅵ),3,3′-dimethylellagic acid-4-O-sulphate(Ⅶ),and(+)-isolariciresinol-9-O-?-D-xylopyranoside(Ⅷ).Conclusion Compound Ⅶ is a new natural product,the other seven compounds are obtained from this plant for the first time.

Objective To study the relationship of high-sensitivity C-reactive protein (hs-CRP),homocysteine (Hcy) and blood pressure variability in elderly patients with essential hypertension.Methods As the total coefficient of variation of 24-hour systolic blood pressure variability(BPsVC),the patients with essential hypertension were divided into two groups:the high-BPsVC group(n =72 cases) and the low-BPsVC group(n =48 cases).The hs-CRP and Hcy in serum in patients were detected.Results The hs-CRP were (2.52 ± 0.78) mg/L in the high-BPsVC group and (1.59 ± 0.61) mg/L in the low-BPsVC group.There was a significant difference (t =0.2766,P ＜ 0.05).The Hcy were(15.6 ±6.2) μmol/L in the high-BPsVC group and(13.7 ± 5.9) μmol/L in the low-BPsVC group.There was a significant difference(t =0.3742,P ＜ 0.05).Condusion There was a close relationship between hsCRP,Hcy and blood pressure variability in elderly patients with essential hypertension.

Objective To study the effectiveness of valsartan on the insulin resistance and high-sensitivity C-reactive protein(hs-CRP) of patients with essential hypertension.Methods One hundred and thirty patients with essential hypertension were randomly divided into two groups,the control group (n =65 cases) and the treatment group (n =65 cases).The patients in the control group were treated through amlodipine,while the patients in the treatment group were treated valsartan.They were all treated for six months.The insulin resistance(IR) and hs-CRP were compared.Results The systolic blood pressure and diastolic blood pressure were decreased after treatment in both groups (t =6.3706,6.4418,7.0519,7.2577,all P ＜ 0.01).There wasn't a significant difference between two groups (P ＞0.05).The IR and hs-CRP were decreased in both groups (t =6.3478,1.9977,4.0581,12.0722,all P ＜ 0.01).There was a significant difference between two groups (t =7.6049,4.6893,all P ＜ 0.05).Conclusion Valsartan can improve the insulin resistance and hs-CRP,which weren't dependent on lowing blood pressure.

The aim is to study the intestinal absorption of different combinations of active compounds out of Gegenqinlian decoction. Rat single pass intestinal perfusion model with jugular vein cannulated was used. Samples were obtained continuously from the outlet perfusate and the mesenteric vein. The levels of puerarin, daidzin, liquilitin, baicalin, wogonoside, jatrorrhizine, berberine and palmatine were determined by LC-MS/MS and their permeability coefficients were calculated. The results showed that Glycyrrhiza could promote the absorption of the active ingredients in Pueraria which is the monarch herb; meanwhile, Pueraria also played a role in promoting the absorption of liquilitin. Based on the Gegenqinlian decoction and the different combinations experiments, the results concerning the absorption of baicalin and wogonoside were as follows. For baicalin, Pueraria and Glycyrrhiza could promote its absorption and the effect of Pueraria was more obvious. For wogonoside, Pueraria could also promote its absorption, while Glycyrrhiza played a opposite role. Pueraria and Glycyrrhiza both played a part in promoting the absorption of jateorhizine, berberine and palmatine, the effective compounds in Coptis.

Objective To evaluate operative methods and the clinical effect for comminuted calcaneal fractures.Methods 21 cases(24 feet)of comminuted calcaneal fractures were treated by one stage bone grafting plus open reduction and internal fixation with plastic calcaneus titanium plate.The fractures were classified according to the Sanders system into Sanders Ⅱ,Ⅲ and Ⅳ subgroups.Results All cases were followed up for 9～20 months(14months in average).All cases recovered with good healing of fracture.According to Maryland score,the results were excellent in 14 feet,good in 7 feet,fair in 2 feet,and poor in 1 foot.The excellent and good rate was 87.5％.Conclusions By using the method of internal fixation with plastic calcaneus titanium plate,fracture site can be well exposed,which is helpful to recover calcaneus to its normal length,width,height,Gissane angle and Bohler angle.A reliable internal fixation is helpful for early stage functional excise after surgery,which can maximize the recovery of the ankle function.

AIM: To study the inhibitory effects of domestic leuprolide acetate microsphere (LE-ms) on the growth of explanted endometrium in the rat models of EMT and its possible mechanisms. METHODS: The 60 rats models of EMT were induced surgically by the Jones method. Then the animals were treated with LE (20 ?g? -1?d -1,28 d,sc) , enanton(20 ?g? -1?d -1,sc) and domestic LE-ms (2, 20, and 200 ?g㎏ -1?d -1,sc), respectively; another 30 rats were divided into sham group(N.S, 1 ml?kg -1?d -1,21 d,sc), EMT+ LE group(100 ?g?kg -1?d -1,21 d,sc)and EMT group(N.S, 1 ml?kg -1?d -1,21 d,sc). At the same time, estrous cycle was monitored daily by examination of vaginal cytologic smears. After 3 weeks, blood was drawn and the serum estradiol concentration was assayed. The volume of endometrial implant was assessed. Lateral uterus, bilateral ovary, thymus and spleen were weighed. The NK cell cytotoxicity in the spleen was evaluated with lactate dehydrogenase (LDH) release assay. RESULTS: Implants in control group continued to grow, while those in groups treated with the drugs showed remarkable atrophy. The inhibitory rates were 87.2%, 78.3%, 57.3%, 89.0% and 94.7%, respectively. The regular estrous cycle of the model rats was abolished and serum estradiol reduced (P0.05); and the NK cells activity was enhanced(P

Aim To study the effect of leuprorelin acetate microspheres (LE ms) on endometriosis in rats, and compare the efficacy of material drug (LE), domestic and imported LE ms (enanton). Methods Endometriosis was induced by endometrial implant in rats. Then the animals were treated with LE (20 ?g?kg -1 ?d -1 ? 28 d ,sc), enanton(20 ?g?kg -1 ?d -1 ,sc)and domestic LE ms ( 2,20,200 ?g?kg -1 ?d -1 ,sc). Results Implants in control group continued to grow, while those in groups treated with drugs shrinked significantly, and domestic LE ms could produce dose dependent inhibitory effect on the growth of endometrial implant in rats. Conclusion The domestic LE ms at the single dose of 20 ?g?kg -1 ?d -1 has the same effectiveness as enanton and routine injection with the same does of LE for 28 days.

Objective To investigate the effect of the combined use of ultrasound (US) elastography and color Dop-pler on the differential diagnosis and TNM staging of benign or malignant breast neoplasm. Methods A cohort of 58 biop-sy-proved cases, who underwent US elastographic and Doppler US imaging, were included in the current study. The pathological results after operation were considered the‘gold standard’of diagnosis. ROC curves were used to compare the changing rates of the diameter and area, elastographic indices and Doppler blood flow score in diagnosis of breast cancer. Re-sults Compared with other indices, the area changing rate showed the highest diagnostic ability with the AUC=0.914. More-over, the combined use of the elastography and color Doppler US increased the AUC to 0.981. The elastographic indices for neoplasm in the advanced-stage (stageⅡ~Ⅳ) were high than those of early-stage (stage 0~Ⅰ). The elastographic and Dop-pler scores increased with the progress of the TNM stages. Conclusion The changing rate of elastographic area can be used to accurately diagnose the breast neoplasm. Moreover, the combined use of US elastography and color Doppler US can in-crease the accuracy of the diagnosis, and may also provide useful information for the TNM staging.

[ABSTRACT]AIM:ToinvestigatetheroleofquercetinintheapoptosisofhumanbreastcancercelllineMCF-7 and the association with Fas/Fas ligand (FasL) pathway.METHODS: The apoptosis model of MCF-7 cells was estab-lished by the induction with quercetin .The morphological characteristics of apoptotic MCF-7 cells were observed under transmission electron microscope .The apoptotic rates and alternation of mitochondrial membrane potential (Δψm ) in the MCF-7 cells were measured by flow cytometry using fluorescein labeled Annexin V-FITC/PI and JC-1, respectively.FasL neutralizing antibody was applied to block the apoptosis .The expression of Fas/FasL on the cells was detected by immuno-fluorescence technique and flow cytometry , respectively.The influence of SB203580 (an inhibitor of p38 MAPK) on the expression of Fas/FasL was also examined by flow cytometry .The protein levels of p 38 MAPK and p-p38 MAPK were de-termined by Western blot .RESULTS: The phenomenon of nuclear condensation and marginalization in the MCF -7 cells treated with quercetin at 80.0 μmol/L for 48 h was observed under transmission electron microscope .Compared with the control cells , theΔψm was decreased by 17.4%, 44.3% and 68.9% in the MCF-7 cells treated with quercetin at 80.0μmol/L for 24 h, 48 h and 72 h, respectively .The apoptotic rates of MCF-7 cells treated with quercetin at 80.0 μmol/L for 24 h, 48 h and 72 h were (10.2 ±3.3)%, (28.9 ±7.5)%and (39.2 ±8.9)%, respectively.However, the apop-totic rates were decreased to (8.2 ±2.8)%, (19.2 ±5.3)% and (22.5 ±6.9)% after the cells were pretreated with FasL neutralizing antibody , respectively .When MCF-7 cells were treated with quercetin for 24 h, 48 h and 72 h, Fas/FasL expression rates were increased in a time-dependent manner , which were largely inhibited by SB203580.The protein level of p38 MAPK was not changed obviously , but the protein level of p-p38 MAPK was significantly increased at 48 h and 72 h.CONCLUSION: Quercetin up-regulates the expression of Fas/FasL on MCF-7 cells, and induces apoptosis via Fas/FasL pathway .Meanwhile , p-p38 MAPK is potentially critical signaling molecule for up-regulating the expression of Fas/FasL.