Cytosine deaminase (CD) gene is a kind of suicide gene, cytosine deaminase can catalyze the conversion from 5 - fluorocytosine (5 - FC) into 5 - fluorouracil (5 - FU), this combination therapy (CDgene/5 - FC) were widely used in cancer therapy. The results of many researches in different cancer cells have demonstrated that it can inhibit tumor growth significantly. The mechanism includes 5 - FU's direct killing tumor cells and its bystander effect. It can also be used as a potential purging method for autologous hematopoietic cells transplantion after chemotherapy in breast cancer patients. CD/5 - FC combined with other therapies would be an important future research approach.

Objective:To enhance the CT department full-time staff professional quality. Methods: Summary according to the CT room personnel in CT department working experience. Results: Eliminate or reduce the CT enhancement in the accident.Conclusion: CT room staff should have good communication and coordination ability, rich clinical experience, skilled professional skills, a high degree of vigilance and a strong sense of responsibility, eliminate their concerns, to implement CT successfully enhance the inspection, the prevention of medical risk.

Objective To observe the vascular collagen remodeling, apoptosis and Bcl-2/Bax of vascular smooth muscle cells in carotid artery on SHR . Methods 12 SHR treated with Irbesartan ,12 SHR without treated Irbesartan ,12 wistar rats were normotensive control group. The vascular volume fraction of Collagen were determined by picrosirius red staining .The expression of the proteins Bcl-2 and Bax were determined by Immunhistochemia,VSMC apoptosis was identified by in situ TDT-mediated dUTP nick end labeling(TUNEL). Results Significant Fibrosis exists in carotid artery of SHR. After 20 week's treating, the vascular volume fraction of Collagen and the ratio of wall/cavity were descended, but weren't parallel. The expression of the protein Bcl-2 and APOI were higher than the subjects of normotensive control, its reduced after treating; The expression of the proteins Bax was similar with control, it increased after treating. Conclusions The results suggest that vascular collagen remodeling exist, its maybe related with apoptosis , the proteins Bcl-2 and Bax of VSMC. The Irbesartan can treat it.

AIM: To establish the methods for determining borneol,isoborneol,muscone,benzyl benzoate in Shexiang Baoxin Pills. METHODS: Turbomass GC-MS instrument was used;the selective ion was applied for detecting borneol,isoborneol,muscone,benzyl benzoate.DB-5 capillary column(0.1 ?m?0.25 mm?30 m).The column temperature was rose by program from 80 ℃ to 260 ℃;the quantiative ion m/z 95 used for borneol and isoborneal,m/z 238 for musone,m/z 212 for benzyl benzoate;Shexiang Baoxin Pills were diluted with hexane. RESULTS: The linear ranges of borneol,isoborbeol,muscone,benzyl benzoate were within 0.020-20.0 ?g/mL(r=0.999 3),0.024-24.0 ?g/mL(r=0.999 0),0.029-28.8 ?g/mL(r=0.999 1),0.050-50.0 ?g/mL(r=(0.999 2)) respectively.The average recoveries of borneol,isoborbeol,muscone,benzyl benzoate were 97.00%,(96.30%,) 96.85%,98.20%,respectively. CONCLUSION: The method is simple accurate and highly sensitive and suitable for Shexiang Baoxin Pills.

Objective:To establish the method of determination of the contents of Danshensu and Protocatechuic aldehyde in Huoxue Mixture simultaneously by RP HPLC.Methods:The determination was carried out with Inertsil ODS column. The mobile phase consisted of methanol 0.5% HAc (22∶78) and UV detection wavelength was 280nm. Results:The average recovery of standard addition were 96.7%, RSD =1.12% ( n =5) for Denshensu, and 97.0 %, RSD =1.53% ( n =5) for Protocatechuic aldehyde, There was a good linear relationship between the absorption area value and the concentration in the range of 1.25～10?g for Deshensu and 0.25?g～2.0?g for Protocatechuic aldehyde.Conclusion: The method is simple, acurate, reproducible and can be used for content determination of Huoxue Mixture.

Objective:To establish the determination of the contents of ferulic acid and paecniflorin in Huoxue Mixture Radix Salviae Miltiorrmizae, Radix Angelicae sinensis, Radix Paeoniae Rubra cortex Moutan, Radix Astragali and caulis Spatholobi simultaneously by HPLC.Methods:The determination was carried out with ZORBAX *ODS column. Chromatographic condition of ferulic acid: the mobile phase consisted of methanol 1%HAc (42∶58) and UV detection wavelength was at 313nm. Chromatographic condition of paecniflorin: the mobile phase consisted of methanol water (25∶75) and UV detection wavelength was 230nm.Results:There was a good linear relationship between the absorption peak area and the concentration in the rang of 0.125～1.0?g for ferulic acid and 0.25～2.0?g for paecniflorin, respectively.Conclusion:The method is simple, acurate, reproducible and can be applied for content determination of Huoxue Mixture.

With high incidence and prevalence,high costs and poor outcome.Optimal dietary and nutrition management is an essential part in managing chronic kidney disease (CKD) and is associated with better patient outcome.An appropriate dietary and nutrition management should be based on chronic disease management model,provided by a multidisciplinary team,and implemented continuously with patient education,evaluation and goal-setting,and finally improve patients' clinical outcomes.

Objective To observe the immune regulatory effect of Marasmius androsaceus (MA) polysaccharide in normal mice. Methods The experimental mouse was injected with high,middle,and low dosages of marasmius androsaceus polysaccharide(5,10,20 mg?kg-1?d-1) in the abdomen (i.p.) ,and the comparison group was given the same volume of saline water i.p. once a day. After treatment for 14 consecutive days,the blood cell count instrument was used to measure the mice hemogram,the phagocytic function of peritoneal macrophage and the ability of carbon granular clearance were observed.,the ability of the spleen lymphocytes proliferation was tested by MTT,and the hemolysin production test was used to evaluate the influence of MA polysaccharide on specific humoral immunity in mice. Results Marasmius androsaceus polysaccharide at middle and high dosages can increase the number of lymphocytes and moncytes in normal reference,enhance the phagocytic function of peritoneal macrophage,promote the carbon granular clearance,accelerate the proliferation of T lymphocyte,enhance the specific humoral imnune function. Conclusion Marasmius androsaceus polysaccharide has certain potentiating effect on the phagocytic function and immunity regulation.

AIM:To establish quality standard for Compound Shenyi Granule(Fructus Jujubae,Radix et Rhizoma Glycyrrhizae,Radix et Rhizoma Salviae miltiorrhizae,Radix Rehmanniae).METHODS:Ziziphus jujuba Mill、Glycyrrhiza uralensis Fisch、Salvia miltiorrhiza Bge、Rehmannia glutinosa Libosch.were identified by TLC.Glycy-rrhizic acid and glycyrrhetinic acid were determined by HPLC.The chromatographic separation was performed on an Agilent ZORBAX Eclipse?DB-C_ 18(4.6 mm?250 mm,5 ?m)column by a gradient elution with methanol and 2.5 % acetic acid at a flow-rate of 1.0 mL/min;the detection wavelength was set at 254 nm.RESULTS:TLC spots developed were fairly clear and the blank test showed no interference.The linear rangers were 1.135-9.08 ?g(r=0.999 3) for glycyrrhizic acid,0.71-5.68 ?g(r=0.999 7) for glycyrrhetinic acid,respectively.CONCLUSION:The methods is effective for the quality control of Compound Shenyi Granule.

AIM:To establish the chromatography fingerprint of the constituents of Melia toosendan Sieb from different areas in order to provide a base for the identification of its quality. METHODS: A gradient separated method was applied. Column:Inertsil ODS-3 C_ 18 ,mobile phase:acetonitrile-water,detection wavelength:270 nm,flow rate:1.0 mL/min,column temperature:25 ℃. RESULTS: To establish the chromatography fingerprint of the constituents of Melia toosendan Sieb., make the technical parameters for its quality controlling,and mark 39 main peaks as its characteristic fingerprint. CONCLUSION: The distribution of constituents of Melia toosendan Sieb differ a little from different areas, but the propotion of the constituents differ greatly, with the Melia toosendan Sieb.from the same area , the distribution and propotion differ a little. This method is reproducible, simple and easy, and can be use to provide a base for the quality control of Melia toosendan Sieb.