1.X-ray Diagnosis of Neonatal Respiratory Distress Syndrome(NRDS) (An Analysis of 20 Cases)
Jicheng DU ; Xingzhong QI ; Fuchun LI ; Haibin ZHOU
Journal of Practical Radiology 1992;0(11):-
Objective To improve the knowledge and diagnostic ability of imagiology of NRDS.Methods The clinical data and X-ray features in 20 cases with NRDS who had complete materials were analysed.Results According to X-ray manifestations all cases were divided into four stages:Ⅰ stage(n=3),Ⅱ stage (n=5),Ⅲ stage (n=8) and Ⅳ stage (n=4).Of them,three cases were in company with aspiration pneumonia,four cases with infective pneumonia,five cases with air leak and two cases with intra-alveolar hemorrhage.Typical X-ray features included:the pulmonary lucency decreased,wide-ranging net and grain shadowes of high density,and in companing with a lot of air bronchus sign.Conclusion X-ray plain film,especially computed radiography is the most important imaging method in diagnosis of NRDS.
2.Reconstruct coronoid process of ulna with ilium-fascia lata complex tissue
Xianglun CHEN ; Shuyuan ZHENG ; Shuqiang MA ; Zengrong WANG ; Xingzhong HUANG ; Qi SHEN
Chinese Journal of Primary Medicine and Pharmacy 2010;17(13):1762-1763
Objective To analyze and explore the operative effect of coraccid process basilar part fractures combined injury of ligamental structure. Methods Use ilium-fascia lata complex tissue to reconstruct coronoid process of ulna and medial collateral ligament, recoverd anterior and wall horn anatomic structure of elbow joint. Total 9 cases of ulna coracoid process fractures concomitancy with elbow posterior dislocation . Among these one with olec-ranon fracture,five patients with head of radius fracture. Anterior trance joint approach was used,removal bone chips, restore defect with ilium-fascia lata complex tissue after cuting and trimming. Once reduction was achieved,fixed with screw. Repaird the collateral ligament and anterior joint capsule use the fascia lata, If there were combined fracture of the radius head or olecranon fracture,fixed through lateral approach,All injured extremities were treated with a posterior plaster splint with functional position for 3 weeks followed by elbow rehabilitation training. Results According to Moneys evaluation method,2 patients were classified as excellent ,5 as good,l as fair and 1 as poor. The excellent and good rate was 77. 8%. Two patients with gently ossifying myositis but no patients with wound infection,internal fixation loosening or break,elbow joint destabilizing orcorpus libemm. Conclusions Coracoid process basilar part fractures was simultaneous with the instability of osteal frame and soft tissue. To attain the favourable anatomical foundation for elbow joint functional recovery, it should rebuild the height and shape of coronoid process first and think highly of repair or rebuild medial collateral ligament and joint capsule. It reconstructed the coronoid process osteal frame and soft tissue instability at the same time to use ilium-fascia lata complex tissue. The operative procedure is simplify with reliability effect and fine functional rehabilitation.
3.Expression and crystallographic studies of a fungal immunomodulatory protein LZ-8 from a medicinal fungus Ganoderma lucidum.
Min AN ; George Fu GAO ; Jianxun QI ; Feng LI ; Xingzhong LIU
Chinese Journal of Biotechnology 2010;26(11):1563-1568
LZ-8 protein, isolated from a well known Chinese traditional medicinal fungus Ganoderma lucidum, is the first member of fungal immunomodulatory protein, members of which have been isolated from a variety of medicinal and edible mushrooms in the last two decades. The protein plays a multifunctional and important role in modulating immune system. In this report, in order to get LZ-8 protein crystals, the LZ-8 gene was expressed and purified by affinity chromatography, gel filtration chromatography and anion exchange chromatography subsequently. The protein was then crystallized using the hanging-drop vapour-diffusion method. The LZ-8 crystals were obtained and the phase information was calculated by X-ray diffraction. The resolution of LZ-8 crystals is 3.2A. This study will provide an insight into the structure of fungal immunomodulatory proteins.
Animals
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Crystallography
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Fungal Proteins
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biosynthesis
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genetics
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immunology
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Ganoderma
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genetics
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metabolism
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Genetic Vectors
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genetics
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Immunologic Factors
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biosynthesis
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genetics
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Mice
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Recombinant Proteins
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biosynthesis
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genetics
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immunology