Abstract: To investigate the status and influencing factors of tooth loss among the elderly population in China, so as to provide insights into early prevention of tooth loss. Methods: Based on the Chinese Longitudinal Healthy Longevity Survey (CLHLS), data of the elderly people aged 65 years and older who participated in the survey in 2018 were collected, including demographic information, lifestyle, dietary habits, medical history, number of teeth remaining and self-assessment indicators. The status of tooth loss among the elderly were described based on the standard of 28 teeth, and factors affecting tooth loss were analyzed using a multivariable ordinal logistic regression model. Results: A total of 5 260 people was recruited, including 3 346 males (63.61%) and 1 914 females (36.39%). The median age was 78.00 (interquartile range, 18.00) years. The median number of teeth remaining was 12.00 (interquartile range, 23.00), and the median number of missing teeth was 16.00 (interquartile range, 23.00). Multivariable ordinal logistic regression analysis showed that the elderly who were female (OR=1.270, 95%CI: 1.098-1.470), had advanced age (OR=1.090, 95%CI: 1.083-1.098), lived in rural areas (OR=1.369, 95%CI: 1.165-1.610) and urban areas (OR=1.208, 95%CI: 1.027-1.422), smoked (past, OR=1.471, 95%CI: 1.240-1.746; always, OR=1.545, 95%CI: 1.300-1.838) and brushed teeth less than once a day (OR=1.791, 95%CI: 1.488-2.160) had more missing teeth; while the elderly who had high levels of education (junior high school, OR=0.819, 95%CI: 0.681-0.972; high school, OR=0.626, 95%CI: 0.507-0.771; college and above, OR=0.468, 95%CI: 0.334-0.657), kept physical exercise (OR=0.840, 95%CI: 0.736-0.958) and were overweight/obese (OR=0.868, 95%CI: 0.769-0.980) had less missing teeth. Conclusion Gender, age, residence, educational level, smoking, physical exercise, teeth brushing frequency and overweight/obese are the influencing factors for tooth loss among the elderly in China.

Objective To explore the clinical significance of coagulation factor and fibrinolytic indexes in viral hepatitis and pregnant hepatitis. Methods 142 inpatients with viral hepatitis classified into five subgroups (acute hepatitis; mild chronic hepatitis; chronic moderate/severe hepatitis; cirrhosis and severe hepatitis),and 35 cases of pregnant hepatitis were measured eight indexes of FV:C、FVⅡ:C、FX:C、PL:A、t-PA:A、PAI:A、D-D和FDP respectively and the results were compared with that of 90 cases from normal control group and 32 cases of normal pregnant women. Results Compared with control, plasma FV: C increased evidently in acute hepatitis (P

Bronchopulmonary dysplasia (BPD) is a commonly seen chronic lung disease in preterm infants.Although its etiology and pathophysiology have not been fully elucidated, it was thought to cause bronchoalveolar arrest.Autophagy is a certain programmed cell death process, which can achieve the renewal of organelles and metabolic needs.It is reported that autophagy is involved in all stages of lung development during the fetal period.The imbalance of autophagy plays an important role in the process of BPD.Furthermore, the appropriate regulation of autophagy could effectively improve lung injury.Now, the recent advancements of the role of autophagy in BPD is summarized in this review.

ObjectiveTo investigate the effects of pre-infusion of hypertonic sodium chloride hydroxyethyl starch 40(HH40)injection in prevention for transurethral electroresection of the prostate syndrome(Tuns).MethodsSixty patients with benign prostate hyperplasia(ASA)grades Ⅱ toⅢwere recruited in the study and randomly divided into two groups,one injected intravenously with HH40 at dose of 5 ml/kg by drip after sursical operation a8 experiment and the other injcoted with the same volume of Ringer's solution as control Mcan arterial pressure,heart rate,saturation of blood oxygen(SaO2)and central venous pressure were monitored for all the patients.In addition.serum concentrations of sodium,potassium,chloride and serum osmolality were determined before(T0)and 30 min(T30)and 60 min(T60) after operation,respectively,as well as pH valRe and partial pressure of carbon dioxide(PgCO2)in the gastric mucosa were determined by a Tonocap monitor.Occurrence of TURS and use of atropine,ephedrine and rinse solution were documented.Resuits TURS occurred in four patients in the eontrol group during surgical operation,whereas uo TUns occurred in experimental group.Serum sodium concentration was (126.5±13.3)mmoL/L at T60 in control group,significantly lower than that in experimental group(P＜0.05),while intragastric pH value and PgC02 level were(7.26±0.04)and(54±9)mm Hg[(7.2±1.2)kPa],respectively in controls,significantly higher than those in experimental group(P＜0.05).ConclusionsPre-infusion with HH 40 can improve tissue perfusion and prevent effectively from TURS in patients with transurethral electroresection of the prostate.

OBJECTIVE:To explore the role of clinical pharmacists providing pharmaceutical care for hemodialysis patients complicated with subacute infective endocarditis(IE). METHODS:Clinical pharmacists participated in the anti-infection treatment for a hemodialysis patients complicated with subacute IE,according to the antimicrobial spectrum,laboratory and imaging find-ings,and patient’s condition changes,assisted physician to optimize the regimen,clinical pharmacists suggested to give imipenem cilastatin sodium after hemodialysis,adjust the initial dose of teicoplanin and give 1 g vancomycin firstly,and maintained 0.5 g af-ter hemodialysis,then adjust its dose based on blood plasma concentration;during treatment,clinical pharmacists closely observed the treatment effect and adverse reactions,providing blood plasma concentration monitoring,medication reminding and medication education. RESULTS:Physicians adopted parts of suggestions of clinical pharmacists,no fever was found,hemogram returned to normal,no abnormal echocardiography,and patient discharged. CONCLUSIONS:Clinical pharmacists guarantee the safety and ef-ficacy of drug use by adopting dose of anti-infection drugs,evaluating efficacy,monitoring adverse reactions and vancomycin plas-ma concentration,and assisting physicians to optimize treatment regimen.

Objective To investigate the protecting mechanism of heat stress pretreatment on acute lung injury(ALI). Methods The oleic acid ALI mouse model was built to dynamically observe the binding capacity and the binding affinity of glucocorticoid receptor(GR),the levels of GR,heat shock protein 90(Hsp90)and Hsp70 before and after hyperthermic stress pretreatment. Results Heat stress pretreatment had significant protective effect on ALI.Western blotting showed insignificant changes of GR levels but progressive increase of level of Hsp70 and Hsp90.Heat stress pretreatment exerted insignificant effect on Bmax and Kd of GR,shown by radio ligand binding assay after ALI. Conclusion The protective effects of heat stress pretreatment on ALI of mouse may relate to its ability of keeping stable GR level and increasing levels of Hsp70 and Hsp90.

Objective To explore the role of H19 imprinting in etiology of pre-eclampsia. Methods Placentas of 24 women with pre-eclampsia (3 with mild pre-eclampsia and 21 with severe pre-eclampsia) and 50 healthy pregnant women at full term (control) were collected during selected cesarean delivery between August 2007 and March 2008. The statuses of H19 imprinting with placental tissues from normal pregnancy and patients with pre-eclampsia were identified upon polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The systolic and diastolic pressure were analyzed in H19 heterozygotic women. Results (1) There were 20 (40%) heterozygotes in 50 cases placenta tissues of the third trimesters, 11 (45%) heterozygotes in 24 cases placenta tissues of pre-eclampsia, There were no significant difference between two groups ( P > 0.05 ). (2) All 20 heterozygotes in placenta tissues of the third trimesters are exclusively monoallelically expressed, while 5 cases (45%) in 11 heterozygotes of pre-eclampsia are biallelically expressed (loss of imprinting, LOI). There were significant difference between two groups (P < 0. 01 ). (3) The values of systolic and diastolic pressure of patients with monoallelic expression of H19 were (171 ±9) mm Hg (1 nun Hg =0.133 kPa) and ( 104±8) mm Hg, the values of systolic and diastolic pressure with biallelic expression were ( 194±21 ) mm Hg and ( 124±18) mm Hg. There were significant difference between two groups (P<0.05 ). Conclusion LOI of H19 can be identified in pre-eclamptic placentas and is associated with maternal blood pressures, which implies the involvement of H19 gene LOI in the pathogenesis of pre-eclampsia and its potential relationship with the severity of the disease.

Objective To obtain the expression pattern of imprint gene H19 in JEG-3 cell in order to explore the regulation mechanism of H19 on trophoblast cellular biological behavior .Methods After correct identification with sequencing for the recom-binant eukaryotic expression plasmid pRc/CMV which including the whole length of H19 cDNA ,the plasmid was transfected to the cell line JEG-3 .The expression of H19 mRNA was observed and the gene expression profile of three groups of JEG-3 cell were de-tected with Affymetri :U133 plus 2 .0 Array .Results After being transfected with target H 19 gene ,the expression of the mRNA level was significantly increased compared with control group .And the gene expression profile was changed significantly .19 genes were up-regulated ,77 genes were down-regulated .Expression levels of HES1 gene which being choosed as a different expression gene were detected by fluorescence quantitative PCR in severe preeclampsia placenta tissue and normal late pregnant placenta .The expression level of HES1 mRNA in severe preeclampsia placenta decreased significantly than normal late pregnant placenta tissues . Conclusion Many genes induced by H19 have been screened by high-throughput gene chip method .It provides the experimental ba-sis for advanced studying the regulation the cellular biological behavior with H 19 gene .

Objective:To improve the quality and efficiency of pharmaceutical care of clinical pharmacists by standardizing phar-maceutical care process using tracking table design for clinical drug therapy. Methods: The experience and skills of pharmaceutical care performed by clinical pharmacists in endocrinology department were summarized and the tracking table for clinical drug therapy was designed, which could provide information for patients clearly and concisely, and make the process of pharmaceutical care more system-atical. Results:After using the tracking table, clinical pharmacists improved work efficiency significantly. In addition, the average hospitalization, average hospitalization expenses and drug proportion significantly reduced resulting in higher satisfaction of patients. Conclusion:The standardized pharmaceutical care process performed by clinical pharmacists in endocrinology department makes phar-maceutical care more specific, comprehensive and convenient.

OBJECTIVE:To strengthen the supervision of clinical antibiotics use through establishing electronic consultation system. METHODS:Based on hospital information system(HIS)and antibiotic management system,consultation system for anti-biotics of special use(ASU)was established,and the effects of the system after used(Jan. 2014)were also evaluated. RESULTS:Real-time consultation of ASU medical orders and related consultation results inquiry and statistics could be achieved through de-signing ASU consultation application form,establishing consultation experts and confirming their working content,adding statistics function,etc. The consultation have been finished 8275 times from 2014 to 2015. The inspection rate for pathogenic microbes in ASU patients rose from 78.68% in 2013 to 83.90% in 2015. CONCLUSIONS:The establishment and application of ASU consulta-tion system can improve consultation efficiency and quality,so as to promote rational use of antibiotics in the clinic.