Wistar rats pretreated with BCG, dexamethasone or carbon particles respectively were inoculated with P. yoelii sporozoites via caudal vein. 42 hours after inoculation, the animals were killed to examine the effects of these 3 agents on the invasion of sporozoites and the development of exoerythrocytic forms (EEF) .It was found that in the carbon group about 30% of the kupffer cells contained the particles, and the density of EEF apparently decreased as compared with that of the control. In the BCG group, a dosage of 6 ? 107 units also caused a marked decrease of EEF density, an,d the parasites were smaller with a higher rate of degenerated forms as compared with those of the control, In the dexamethasone group, however, the EEF density increased markedly up to the level 1.5-2 times higher than that of the control. In addition the EEF were larger and more numerous with vacuoles and some lobulated. The mechanism of these facts were discussed.

Objective: To compare the anticoagulation effect of local anticoagulation with citrate in the hemofiltration for sepsis patients with acute renal injury and observe the safety of anticoagulation to provide the basis for clinical anticoagulation of hemofiltration.Methods: Totally 72 patients with sepsis acute kidney injury in the hospital were randomly divided into the control group and the study group with 36 ones in each according to the single and double number.The control group and the study group was respectively treated with heparin sodium and citric acid for anticoagulation with the treatment course of 7 days.The life of filter tube and filter of the two groups were recorded, and the coagulation grade of filter was assessed.The levels of serum creatinine (Scr), blood urea nitrogen (BUN), pH, serum sodium bicarbonate and bicarbonate were measured before and after the treatment.The activated coagulation time (ACT) was measured before the filtration and in 8 h and 24 h after the filtration.The adverse reactions were observed as well.Results: The ratio of blood clotting level at 0-1 grade in the study group was higher than that in the control group, and the ratio of filter coagulation at 2-3 grade in the study group was lower than that in the control group, and the differences were statistically significant (P<0.05).The life of filter pipe and filter in the study group was longer than that in the control group, and the difference was statistically significant (P<0.05).ACTs in 8 h and 24 h after the blood filtration in the study group were longer than those in the control group, and the difference was statistically significant (P<0.05).The levels of BUN and Scr of the two groups after the treatment were lower than those before the treatment, and the differences were statistically significant (P<0.05).There were no significant differences in blood pH, HCO3-, Na + and adverse reactions before and after the treatment between the groups (P>0.05).Conclusion: The application of hemofiltration in the treatment of sepsis patients with acute renal injury can significantly improve renal function.And local anticoagulation has better anticoagulant effect in septic acute renal injury patients with hemofiltration, which can prolong the life of filter tube and filter with higher security.

It is now generally recognized that repairing of defect bones is one of the major issues in bone tissue engineering research.However,nowadays massive bone defect because of injury,infection or tumor removal has not been effectively solved in clinic.One of the key issues in bone tissue engineering is finding suitable biodegradable materials substitutes and scaffolds for seeding cells and guiding the subsequent growth of bone.The design and fabrication of bone tissue engineering scaffolds are overviewed as well as the development of the scaffold materials.

Objective To investigate the effects of rapamycin on the number and function of peripheral blood endothelial progenitor cells (EPCs). Methods Total mononuclear cells (MNCs) were isolated from peripheral blood by Ficoll density gradient centrifugation, and then the cells were plated on fibronectin-coated culture dishes. After 7-day culture, adherent cells were treated with rapamycin in a series of final concentrations of 1.0, 2.0, 5.0?g/ml for 6, 12, 24, and 48h. EPCs were identified as adherent cells double positive for DiLDL-uptake and lectin binding by direct fluorescent staining as demonstrated under a laser scanning confocal microscope. EPCs were further documented by demonstrating the expression of VEGFR-2, AC133 and CD34 with flow cytometry. EPCs proliferation and migration were assayed with MTT assay and modified Boyden chamber assay, respectively. EPCs adhesion assay was performed by replating them on fibronectin-coated dishes, and then adherent cells were counted. In vitro vasculogenesis activity was assayed by in vitro vasculogenesis kit. Results Incubation of isolated human MNCs with rapamycin resulted in a decrease in the number of EPCs, and rapamycin also decreased EPCs proliferative, migratory, adhesive and in vitro vasculogenesis capacity in both concentration and time dependent manners. Conclusion Rapamycin decreases the number, proliferative, migratory, adhesive and in vitro vasculogenesis capacity of EPCs.

Proteome is a discipline which researchs the composition and the dynamic alteration of proteins.As the result of the progression in the clolorcetal cancer,the proteins expression showing a dynamic change process.The technique of proteome can perform qualitative and quantitative analysis for the proteins,and perform contrastive analysis for the normal hunman proteins metabolic.So we can screen the biomarkers that are associated with coloretal cancer progression.The article aims to summarize the proteome in the researching of the colorectal cancer.And summing up the biomarkers that are associated with the diagnosis,prognosis and treatment of the coloretal cancer.

P. yoelii sporozoites(Sp) in Anopheles stephensi were first isolated with low-speed centrifugation and the Sp suspension was subsequantly purified with the density gradient centrifugation. The recovery rates of Sp by the latter method is about 50-75% of that by the former, but few debris could be found in the Sp suspension and the infectivity of the Sp was not weakened as compared with the Sp obtained by low-speed method. Sp would retain partial infectivity, when its suspension was maintained ia medium 199 at 4℃ for 48 hrs. When rats and mice were infected with these Sp, patho-logical changes of hepatocytes such as cloudy swelling or fatty degeneration, which had been evidenced to be induced by mosquito tissues, would be absent or present in th& slightest degree.

Objective To investigate the effect of high glucose on the number and proliferation, migration and adhesion of peripheral endothelial progenitor cells (EPCs). Methods Total mononuclear cells (MNCs) were isolated from peripheral blood by Ficoll density gradient centrifugation, and then the cells were plated on fibronectin coated culture dishes. After 7 days of culture, several groups of attached cells were incubated with glucose in a series of concentrations (15, 25, 35, 45mmol/L) for different durations (6, 12, 24 and 48h). EPCs were characterized as adherent cells which were double positive for DiLDL uptake and lectin binding by direct fluorescent staining demonstrated under a laser scanning confocal microscope. EPCs were further documented by demonstrating the expression of KDR, VEGFR 2 and AC133 with flow cytometry. EPCs proliferation, migration and in vitro vasculogenesis activity were assayed with MTT assay, modified Boyden chamber assay and in vitro vasculogenesis kit, respectively. EPCs adhesion assay was performed by replating MNCs on fibronectin coated dishes, and then the adherent cells were counted. Results Incubation of isolated human MNCs with high glucose concentration decreased the number of EPCs, and this effect was most prominant when glucose concentration was 45mmol/L, and incubated for 24 hours (approximately 1 fold decrease, P

0.05).Compared with SHR group,left ventricular weight mass index decreased significantly in SHR-A group(P

Wound secretion from 20 patients with gaseous gangrene was collected for Gram staining,bacterial culture and drug sensitivity tests.The results indicated that gaseous gangrene was caused by the co-infection of both aerobic and anaerobic bacteria.Gram-negative bacilli were slishtly more common than other aerobic bacteria in gageous gangrene wound,which was different from the findings of ordinary gaseous gangrene.

Objective To investigate the effect of long hairpin RNA ( lhRNA) expression vector targeting HBV X gene ( HBx) on replication of hepatitis B virus ( HBV) and gene expression.Methods Four kinds of small interference RNAs ( siRNAs) were synthesized and lhRNA expression vectors targeting HBx were constructed.Four siRNA oligonucleotides and two lhRNA expression vectors were transfected into HepG2.2.15 cells.HBsAg, HBV DNA in culture supernatants and HBx mRNA in HepG2.2.15 cells were detected by time-resolved immunofluorometric assay, real-time quantitative PCR, and reverse transcription PCR, respectively.Negative sequence group or empty vector group was taken as the control.Independent-samples t test was performed to evaluate the inhibition effect on replication of HBV and gene expression. Results Compared with the negative control, HBsAg, HBV DNA level in culture supernatants and HBx mRNA in HepG2.2.15 cells were significantly decreased after siRNA-1 and siRNA-4 transfected at high concentrations (60 nmol/L or 90 nmol/L) (P<0.05), especially the HBsAg and HBV DNA levels in the siRNA-1 transfection group, which were significantly decreased at 24, 48 and 72 h after transfection ( P<0.05 or P <0.01 ) . Two lhRNA expression vectors ( pMD-HBxlh1 and pMD-HBxlh4 ) were successfully constructed and transfected into HepG2.2.15 cells, HBsAg and HBV DNA level in transfected cells was significantly lower than those in negative control (P<0.05).Conclusion The novel siRNA-1 is confirmed to target HBx gene and lhRNA expression vector targeting HBx can effectively inhibit the replication of HBV and expression of HBV gene.