Medicine,as a practical and experiential science,requires clinicians to understand the disease entity gradually from the clinical practice.Qualified clinicians must have broad and solid basic theory and knowledge,excellent clinical techniques and critical clinical thinking.The clinical experience starts from the clinical thinking process,including the summarization of the knowledge of disease entity and lessons from clinical practice.Clinicians who have learnt correct and reasonable clinical route,thinking process and methods can improve their realization about the disease from perception to rationality and accumulate their clinical experiences.The target of the clinical critical thinking is to guide the diagnosis and management,which is crucial for medical practice,and to help clinicians understand the disease deeply and arrive better decision-making.Case-based discussion and forum is the essential route to improve the ability to think clinically.

Objective To analyze the clinical characteristics and determine the early diagnosis and treatment of mesenterie venous thrombosis (MVT). Methods Clinieal data of 31 cases with MVT were analyzed retrospectively. Results Of all 31cases,19 patients presented acute MVT. Abdominal pain was the first onset symptom and then progressively exacerbated; peritoneal irritation and ascites developed in 57.9% and 68.4 % patients with sign of ascites respectively. 13 patients were diagnosed by abdominal CT scan or selective mesenterie angiography; the detectable rate of CT for acute MVT was 83.3%. The level of plasma D-Dimer was increased in 93.3% patients with acute MVT. 13 patients underwent surgical treatment, among them,6 cases received anticoagnlafion treatment,4 cases recrudersced and 3 died. 12 chronic MVT patients had no symptoms and were identified through abdominal CT 8 cases developed upper gastrointestinal hemorrhage and 2 died. Conclusion The nonspecifie nature of the abdominal symptoms often delays the diagnosis of MVT and leads to high mortality. Abdominal CT is valuable diagnosis method for MVT and anticongulation treatment and operation are effective managements.

Familial nonmedullary thyroid carcinoma means to two or more first-degree relatives of the family with NMTC,in the absence of other known family syndromes (such as Gardner's syndrome,Cowden disease,Carney complex,Werner syndrome,and so on),and common thyroid cancer risk factors including the neck radiation and iodine deficiency,and so on.The genes involved in the pathogenesis of familial nonmedullary thyroid carcinoma (FNMTC) are yet to be elucidated,although some recent studies identified several predisposition loci with a high degree of genetic heterogeneity.Several studies demonstrated that patients with FNMTC have increased rates of multifocal disease,extrathyroidal invasion,and involved lymph nodes compared with sporadic disease.It has been hypothesized that this increased aggressiveness translates into higher recurrence rates and decreased survival of patients with FNMTC.

Object To study the direct effect of quercetin on recombinant human protein kinase CK2 holoenzyme and its kinetics. Methods Recombinant human protein kinase CK2? and ? subunits were cloned and expressed by gene engineering, and were purified. The two subunits were mixed at the same molar ratio, thus reconstituting CK2 holoenzyme, which displayed the maximum bioactivity. The CK2 activity was assayed by detecting incorporation of 32 P of [? 32 P] ATP into the substrate in the various conditions. Results The recombinant human protein kinase CK2 was the second messenger (Ca 2+ , cAMP and cGMP) independent protein kinase, the characterization and function of the reconstituted holoenzyme were consistent with those of native CK2. It was found that quercetin strongly inhibited the holoenzyme activity of recombinant human protein kinase CK2 with an IC 50 of 522 nmol/L, which was much more effective than DRB and A3, known as CK2 special inhibitors. Kinetic studies of quercetin on recombinant human protein kinase CK2 showed: the inhibition was competitive with ATP and noncompetitive with casein. Conclusion Quercetin is a potent inhibitor of recombinant human protein kinase CK2. The inhibition may be another molecular mechanism of antitumor effect of quercetin. This study provides a simple and rapid screening method for the development of more effective inhibitors of recombinant human protein kinase CK2.

Objective: To verify 5-HT4 receptor mRNA expression in intestinal mucosa mast cells (IMMC). Methods: IMMC was isolated from the whole intestines of normal rats by collagenase digestion and purified by percoll. In situ hybridization was performed to detect the expression of 5-HT4 receptor mRNA in IMMC. Results: Evidently positive staining was shown in the cytoplasm of IMMC. Conclusion: We have verified the expression of 5-HT4 receptor mRNA in IMMC for the first time and provided evidence for further research.

AIM　To study the direct effect of tyrphostin AG114 on recombinant human protein kinase CK2 holoenzyme and its kinetics. METHODS　Recombinant human protein kinase CK2 α and β subunits were cloned and expressed by genetic engineering, and purified to homogeneity. The two subunits were mixed at equal molar ratio and reconstituted CK2 holoenzyme, which exerted the maximum biological activity. The CK2 activity was assayed by detecting incorporation of 32P of ［γ-32P］ATP or ［γ-32P］GTP into the substrate in various conditions. RESULTS　The recombinant human CK2 was a second messenger (Ca2+, cAMP and cGMP) independent protein kinase, the characterization and function of the reconstituted holoenzyme were consistent with those of native CK2. AG114 strongly inhibited the holoenzyme activity of recombinant human protein kinase CK2 with an IC50 of 20.8 μmol·L-1, which lay between IC50 of 5,6-dichloro-1-β-D-ribofuranosyl-benzimidazole(DRB) and N-(2-aminoethyl)-5-chloronaphthalene-1-sulfonamide(A3), known as CK2 special inhibitors. Kinetic studies of AG114 inhibition on recombinant human CK2 showed that the inhibition was mixed competitive with GTP and noncompetitive with casein. CONCLUSION　AG114 not only is an effective inhibitor of protein tyrosine kinases, but also is a novel potent inhibitor of protein kinase CK2. The recombinant human protein kinase CK2 might be used as a molecular target for simpler screening method and development of more effective inhibitors of CK2.

Research of cell cycle is the bas is of investigating the organism growth,development,heredity and other medical associated events. The molecular basis of cell cycle control is the regulator control system with CDK-Cyclin-CKI as the core.Protein kinase CK2 is one of the most conservative protein kinase during evolution and more and more researches have proved that protein kinase CK2 plays an important role in cell cycle control.

Objective Zinc sulfate has anti inflammatory action in many animal models, however, the effects of zinc in colitis remained uncertain. The present study was to evaluate the role of zinc sulfate in experimental colitis and probe into its underlying mechanisms. Methods Colitis was induced by administrating 2,4,6 trinitrobenzenesulphonic acid (TNB) rectally in Spragur Dawley female rats. Beginning at the first day of TNB colitis, the rats were treated with zinc sulfate enema once daily for 6 days. The rats were sacrificed at days 8. The effects of zinc sulfate were evaluated by examining mucosal lesion area, mucosal myeloperoxidase (MPO) and superoxide dismutase (SOD) activities, mucosal prostaglandin E 2(PGE 2) and leukotriene B 4(LTB 4) levels. Results TNB induced severe colitis as evidenced by increased mucosal lesion area, mucosal MPO activity and PGE 2 and LTB 4 levels. Six days after the application of the zinc sulfate enema, the mucosal lesion area, MPO activity, PGE 2 and LTB 4 levels were all decreased significantly, except mucosal SOD activity that was remained unchanged after zinc treatments. Conclusions The data suggest that zinc sulfate enemas have an anti inflammatory action on experimental colitis through the mechanism other than increasing SOD activity.

AIM To study the effects and kinetics of sodium quercetin 7 sulphate (SQMS) on recombinant human protein kinase CK2 holoenzyme. METHODS The recombinant human CK2 holoenzyme activity was assayed by detecting incorporation of 32 P of [? 32 P]ATP into the substrate at various conditions. RESULTS SQMS was shown to strongly inhibit the holoenzyme activity of recombinant human protein kinase CK2 with an IC 50 of 1 37 ?mol?L -1 , Kinetic studies of SQMS on recombinant human CK2 showed: the inhibition was competitive with ATP and noncompetitive with casein. CONCLUSIONSQMS is an inhibitor of protein kinase CK2, and the inhibitory action of SQMS on CK2 is competitive with ATP and noncompetitive with casein.