Objective To investigate and analyze the clinical efficacy of LEEP knife combined with chitosan cervical antibacterial membrane in the treatment of chronic cervicitis and to observe the psychological intervention. Methods 100 cases of Chronic Cervicitis Patients in our hospital from January 2015 to October 2016 were selected as the research object. They were randomly divided into the control group and the experimental group, each group had 50 cases. Patients in the control group were treated with LEEP knife surgery, and no drugs were used after the operation. The patients in the experimental group were treated with LEEP knife combined with chitosan and cervical antibacterial membrane. Psychological intervention was carried out, and the mental health of the patients was paid close attention to, and communication and communication with patients were strengthened. The therapeutic effects of the experimental group and the control group were compared and analyzed. Results After the corresponding treatment, the effective rate of the experimental group and the control group was 100.0%. The cure rate of the control group was 80.0%, significantly lower than that of the experimental group(96.0%), the difference was statistically significant (P<0.05). After treatment, the SAS score of the experimental group was (40.1±6.2) points, and the SDS score was (38.4±7.9) points. The scores of SAS in the control group were (49.6±7.2) points, and the SDS score was (48.2±8.5) points. There were three cases of complications in the experimental group, the incidence of complications was significantly lower than that of the control group (20.0%), The difference was statistically significant (P<0.05). Conclusion The clinical effect of LEEP knife combined with chitosan cervical antibacterial membrane in the treatment of chronic cervicitis treated with psychological intervention, high cure rate, low probability of complications, significantly improve patients' negative emotions, has clinical significance.

Objective To study and analyze the psychological intervention methods and effects of mifepristone combined with misoprostol in the treatment of abortion. Methods 200 cases of intrauterine pregnancy were selected from Hangzhou Red Cross Hospital from March 2015 to August 2016. They were randomly divided into the control group and the experimental group, 100 cases in each group. The patients in the experimental group were treated with mifepristone combined with misoprostol, which eliminated the negative emotions such as anxiety and restlessness, and paid close attention to the psychological status of the patients. Patients in the control group were treated with local medication of lidocaine. The clinical effects of the two groups were compared and analyzed. Results after the corresponding treatment, the effective rate of analgesia in the experimental group was 38.0％, and the effective rate was 52.0％. The effective rate of the control group was 16.0％, and the effective rate was 49％. The analgesic effect of the experimental group was significantly higher than that of the control group, and the difference was statistically significant (P<0.05). After treatment, the effective rate of cervical dilatation in the experimental group was 45.0％, and the effective rate was 51％. The degree of cervical dilatation in the experimental group was significantly better than that in the control group, and the amount of bleeding during operation was significantly less than that in the control group, and the difference was statistically significant (P<0.05). Conclusion Misoprostol and mifepristone combined with psychological intervention can greatly improve the clinical effect of artificial abortion and alleviate the pain of patients, it has the significance of further popularization and application.

Objective:To realize prokaryotic expression, purification and identification of 2019-novel Coronavirus (2019-nCoV) nucleocapsid protein (NP), and apply it to the serological diagnosis.Methods:The synthetic 2019-nCoV NP gene was cloned into the prokaryotic expression vector pET28a to construct expression plasmid, and then purified by Ni-chelating affinity. SDS-polyacrylamide gel electrophoresis (SDS-PAGE), indirect enzyme-linked immunosorbent assay (ELISA), Western blot (WB), and immunochromatography were used to test the purified protein. Indirect ELISA reaction conditions were optimized for serum antibody detection.Results:The relative molecular mass of recombinant NP was about 50×10 3 after SDS-PAGE electrophoresis, which was consistent with the expectation. Indirect ELISA and WB results showed that it could specifically bind to the serum of patients infected with 2019-nCoV. The detection limit of NP was 0.2 ng/ml by immunochromatography. The sera from 32 patients infected with 2019-nCoV and the control sera were detected by indirect ELISA, and the results showed that they were clearly clustered. Conclusions:Prokaryotic expression of 2019-nCoV NP has good immunogenicity and can be used for the development of serological diagnostic reagents.