[Objective] To investigate the effects of Naoyi'an (NYA) on the expression of ICAM-1 in the brain of rats with experimental intracerebral hemorrhage.[Methods] The rats model of intracerebral hemorrhage was prepared by type Ⅶ collagenase injected into globus pallidus under stereotaxic apparatus.Then,the expression of ICAM-1 with or without intervention with NYA was determined in the brain of these animals respectively by in situ hybridization and western blot technique.[Results] Among the modeling animals,the expression of ICAM-1 was increased after the operation.In contrast,the expression of ICAM-1 was significantly down-regulated in the rats treated by NYA(P

Objective To study expression change of vascular endothelial growth factor(VEGF) protein and mRNA in rat brain with cerebral hemorrhage and effect of traditional Chinese medicine complex Nao Yi An(NYA) on VEGF expression. Methods The intracerebral hemorrhage(ICH) animal models were made by injecting collagenase type Ⅶstereotaxically into right globus pallidus. The experiment techniques of immunohistochemistry and in situ hybridization were employed to detect the expression variation of VEGF at 3h,6h,12h,1d,4d,7d and 14d after cerebral hemorrhage by positive cell counting as an observation index. Results The VEGF expression in the rat brains was observed at 3h after cerebral hemorrhage, raising at 6h,peaking at 12h and remaining peak until 1d. In the fourth day, VEGF began to decrease and still expressed a little at 7d and 14d.NYA could increase the VEGF expression in rat brains with cerebral hemorhage obviously at 6h ,12h,1d and 4d (P

Objective To establish a hydrogen peroxide-impaired model of rat cerebral microvascular endothelial cells in vitro, and observe the protective effect of serum containing Naoyian. Method Rat cerebral microvascular endothelial cells were treated with six concentrations of hydrogen peroxide at four time points. The optimum injury condition of hydrogen peroxide was determined by MTT chromatometry. Then the cultured cells pretreated with serum containing Naoyian and pyrrolidine dithiocarbamate(PDTC) were interfered with hydrogen peroxide in suitable concentration and time, and OD of the treated cells was measured by MTT chromatometry. Results The cultured cells were injured obviously by hydrogen peroxide in 0 125mmol/L for 30min. The OD of the cells pretreated with 5% serum containing Naoyian and 50?mol/L PDTC were significantly higher than that of the cells without pretreatment (P

As an interdisciplinary of stomatology and space medicine, space oral medicine focuses mainly on oral diseases happened under space environment. With the manned space technology stepping into the new era, space oral medicine has been put under the spotlight. This article will review the historical events on this subject, summarize the newly progress especially on craniomaxillofacial bone, tooth-derived stem cell and oral microbiology researches and still put forward future prospect.
Aerospace Medicine ; Biomedical Research ; Humans ; Mouth Diseases ; Oral Medicine ; Stem Cells ; Weightlessness

OBJECTIVETo clone, express, and purify cyclic diadenosine monophosphate (c-di-AMP) metabolism-related genes from Porphyromonas gingivalis (P. gingivalis) ATCC33277.

METHODSPolymerase chain reaction (PCR) from the genome of P. gingivalis ATCC33277 amplified, the coding regions of pgn0523, pgn1187, and pgn2003 genes. The amplified DNA fragments were ligated with a prokaryotic expression vector pET28a to construct the recombinant expression plasmids pET-pgn0523, pET-pgn1187, and pET-pgn2003. These recombinant plasmids were transformed into Escherichia coli (E. coli) BL21 (DE3) competent cells. The expression of recombinant proteins was induced by isopropyl-β-D-thiogalactoside and detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Proteins were purified using a Ni²⁺ matrix column, and their concentrations were determined by a BCA Protein Quantitative Kit.

RESULTSThe c-di-AMP metabolism-related genes from P. gingivalis ATCC33277 were amplified successfully with the correct molecular size. The recombinant expression vectors were constructed by ligating enzyme-digested PCR products and pET28a vector, and verified by PCR and sequencing. After induction and purification, recombinant proteins were expressed successfully and obtained with the correct molecular size (19.5 x 10³, 39.9 x 10³, 66.0 x 10³). The final protein concentrations were 0.708, 0.523, and 0.861 mg · mL⁻¹ after dialysis.

CONCLUSIONThe c-di-AMP metabolism-related genes from P. gingivalis ATCC33277 are cloned successfully, and their coding products are expressed correctly in E. coli. High-purity proteins are finally obtained. The cloning and purification of these important proteins will help us to further investigate the physiological function and regulatory mechanism of c-di-AMP signaling system in P. gingivalis.

Bacterial Proteins ; biosynthesis ; genetics ; isolation & purification ; Cloning, Molecular ; Dinucleoside Phosphates ; Escherichia coli ; genetics ; Genetic Vectors ; Plasmids ; Polymerase Chain Reaction ; Porphyromonas gingivalis ; genetics ; Recombinant Proteins

0.05), but LA patients had less postoperative pain,less time to ambulation after operation, less time to food intake after operation, less average length of hospital stay,less rate of incision infection and less intestinal adhesion than OA did(all P

OBJECTIVE: To investigate the effects of Xiehuo Bushen Decoction (XHBSD), a compound Chinese herbal medicine, on the survival and differentiation of transplanted neural stem cells (NSCs) in brains of rats with intracerebral hemorrhage, and to explore the mechanism of Xiehuo Bushen formula in promoting the survival of transplanted NSCs. METHODS: NSCs separated from hippocampuses of neonatal SD rats were cultured. Sixty-five panel reactive antibody (PRA) positive SD rats were selected by lymphocytotoxicity methods. The PRA positive rats were made into intracerebral hemorrhagic model and divided into three groups: cerebral hemorrhage group (n=15), NSCs transplanted group (n=25) and XHBSD group (n=25). XHBSD was orally administered after 5-bromodeoxyuridine (BrdU)-marked NSCs were transplanted in brains of rats with intracerebral hemorrhage in the XHBSD group. Rats in the other two groups were administered distilled water. The expressions of interferon gamma (IFN-gamma) and interleukin-4 (IL-4) mRNAs were measured by reverse transcription polymerase chain reaction (RT-PCR); the numbers of BrdU and 200 kD neurofilament (NF200) positive cells were detected by double-labeling immunofluorescence method. RESULTS: The expression of IFN-gamma mRNA was down-regulated significantly in the XHBSD group, but the expression of IL-4 mRNA was up-regulated significantly (P<0.05). The numbers of BrdU and NF200 positive cells were also increased remarkably in the XHBSD group. CONCLUSION: XHBSD can promote the survival and differentiation of transplanted NSCs, which may be related to inducing the expression of IL-4 mRNA and inhibiting the expression of IFN-gamma mRNA.

Objective: To observe the clinical therapeutic effects of supplementing Qi and activating blood circulation method(益气活血法) for critically ill patients.Methods: Ninety critically ill patients with(Qi-deficiency)(气虚) and blood stasis(血瘀) syndromes who diagnosed according to standard in a book named clinical diagnosis and treatment nomenclature of traditional Chinese medicine were randomly divided into the the treatment group(n=60) and the control group(n=30).The general therapy of the two groups was the same.Additionlly,the treatment group was administered Shenmai injection(丹参注射液) and Danshen power(丹参粉针剂),15 days were as one therapeutic course.Results: In the treatment group,the total effective rate of clinical therapeutic effects was 85.00 %;before and after treatment,traditional Chinese medical scores was 38.63?9.08 vs.24.27?7.43,acute physiology and chronic health evaluationⅡ(APACHEⅡ) 18.11?4.54 vs.12.47?1.64,platelet(PLT) count(198.00?54.16)?10~9/L vs.(174.00?40.82)?10~9/L,(haematocrit)(HCT) 0.340?0.049 vs.0.440?0.057,mean cell hemoglobin(MCH)(34.00?3.10)pg(vs.(31.00?1.83) pg).The differences of above parameters were significant between the two groups,and they were superior in the treatment group to those in the control group(all P

OBJECTIVETo establish HPLC fingerprint of Prunella vulgarise for quality control of the herbal medicine.

METHODA sunfire C18 analytical column was used. The mobile phase A was 1% acetic acid, and mobile phase B was methanol. The elution was in gradient mode and detection wavelength was set at 290 nm. The flow rate was 1.0 mL x min(-1) and the column temperature at 30 degrees C. The analysis time was 60 min.

RESULTThe similarity of 10 batches of P. vulgaris was not lower than 0.810. The fingerprints of the herbal medicine were classified P. vulgaris on the results of cluster analysis.

CONCLUSIONThis method is available for quality evaluation and control the quality of P. vulgaris.

Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; chemistry ; Prunella ; chemistry

Objective To investigate the clinical value of splenic autotransplantation in patients with severe splenic trauma. Methods A prospective case-control study were performed in 120 patients with traumatic spleen rupture including the treatment group 72 patients and the control group 48 patients. The treatment group were treated with splenectomy plus spleen autotransplantation and the control group merely under splenectomy. Compare the operation time,operative blood loss,postoperative hospital stay,postoperative complications and the immune indexes before and different period after operation. Results Autologous spleen transplantation takes more time than merely splenectomy(P<0.05),but the operative blood loss,postoperative hospital stay and postopera-tive complications were no significant difference. 1 days after operation,the immune indexes of two groups were significantly lower than those before operation(P < 0.05),and 1 week after operation the immune indexes of two groups were significantly elevated(P<0.05).The immune indexes of the treatment group were better than those of the control group 3 months after operation(P < 0.05),and there was no significant difference compared with preoperative. Conclusion Splenectomy cause the decrease in the immune function,but the immune function can quickly rise to a certain level in short term.The splenic autotransplantation can effectively restore the immune func-tion to the preoperative level.