Objective To investigate the expression of cyclooxygenase-2(COX-2)and Survivin in ameloblastoma (AB) tissues. Methods A total of 60 AB samples (primary AB 40 cases, recurrent AB 20 cases) and 60 normal oral mucosas were collected from the Affiliated Hospital of Chengde Medical College. The AB samples included 12 acanthomatous types, 18 follicular patterns, 18 plexiform patterns, 6 basal cell ameloblastomas and 6 desmoplastic ameloblastomas. The expres?sion levels of COX-2 and Survivin were detected by immunohistochemistry and Western blot methods in AB and normal oral mucosa. The expressions of COX-2 and Survivin were compared between different types and tissues of AB. Results The positive rate of COX-2 was significantly higher in AB (82.7%) than that in normal oral mucosa (10.0%). The positive rate of Survivin was significantly higher in AB (83.3%) than that in normal oral mucosa tissues (16.7%). The expression of COX-2 (0.781±0.142) was higher in AB than that of normal oral mucosa tissues (0.179±0.056). The expression of Survivin (0.447± 0.139) was significantly higher in AB than those in normal oral mucosa tissues (0.072±0.017). There was a positive correla?tion between the expression of COX-2 and Survivin (r=0.778,P<0.05). There were no significant differences in the positive expressions of COX-2 and Survivin between different types of AB (P>0.05). Conclusion COX-2 and Survivin were over-expressed in AB, which may be involved in the occurrence and development of AB.

To study the epidemiology and etiology characteristics of first imported Chikungunya fever case in Henan province, China, 2017. The patient was confirmed by Chikungunya virus (CHIKV) infected as CHIKV ribonucleotide was continuously detected in his serum specimens. BHK-21 cell line was used for virus isolation, the strain was named CHIKV/Henan001/2017. CHIKV/Henan001/2017 belonged to genotype ECSA. The highest ribonucleotide homology sequence of highly conserved region E1 with CHIKV/Henan001/2017 was hk02 strain (99.8%), who was an imported strain to Hong Kong, China, 2016. Epidemiological information and laboratory testing confirmed it was an imported Chikungunya fever case in Henan province, 2017. No secondary case has been reported.

OBJECTIVETo investigate the etiology of severe hand-foot-mouth disease (HFMD) in children in Henan province.

METHODSA total of 244 HFMD cases admitted to a hospital in Zhengzhou from April to June of 2014 were recruited for research sampling, Real-time RT-PCR, virus isolation, VP1 sequencing and alignment methods were used to test the enterovirus-related etiology. SPSS 17.0 was used in performing statistical analysis.

RESULTSThere were 109 severe and 135 mild cases among all the 244 HFMD cases. The number of enterovirus positive stool samples was 229, with positive rate as 93.85%. EV71, Cox A16 and Cox A10 made up 83.84%, 5.68% and 8.30% of the enterovirus etiologicy, strains, respectively. EV71 infection caused 8 HFMD cases with heart-lung failure and 2 death, Cox A10 infection led to 1 HFMD case with heart-lung failure and death. There were statistically differences seen regarding the enterovirus infection rates between severe and the mild HFMD cases (χ(2)=5.312,P=0.021). Statistically significant difference was seen in the constituent ratio of EV71, Cox A16 and the others by Fisher' s exact test (P=0.048). There was statistically significant difference seen between the cardiorespiratory failure rate and the fatality rate by EV71 and Cox A10 infection (χ(2)=0.051,P=0.821; χ(2)=2.198,P=0.138). Cox A10 strains idenfied in Henan in 2014 belonged to genotype 6. The rates on homology of nucleotide and amino acid among the Cox A10 strains in Henan in 2014 were 94.3%-99.7% and 96.3%-100.0% respectively.

CONCLUSIONSEV71 still remained the most common pathogen that causing severe HFMD in children, with the increasing Cox A10 percentage in the pathogens spectrum of HFMD infection. Cox A10 strains in Henan in 2014 belonged to genotype 6. Genotype 6 Cox A10 had appeared and widely distributed in Henan for long time, but not yet variated or reconstructed. Cox A10 infection could lead to cardio-respiratory failure thus called for the monitoring program on non-EV71 and non-Cox A16 enterovirus, especially Cox A10 to be strenthened.

Amino Acids ; genetics ; Biometry ; Child ; Enterovirus A, Human ; classification ; genetics ; isolation & purification ; Enterovirus Infections ; epidemiology ; virology ; Evolution, Molecular ; Genotype ; Hand, Foot and Mouth Disease ; epidemiology ; prevention & control ; virology ; Hospitals ; statistics & numerical data ; Humans ; Real-Time Polymerase Chain Reaction