Objective To analyze the effect of Ligustrazine combined with cephalosporin on the elderly patients with acute attack of chronic bronchitis. Methods From April 2015 to May 2017 selected 72 patients with chronic bronchitis acute attack were divided into group A and groupB according to the different treatment options, 36 cases in each group. Group B were received single cephalosporin antibiotics, while group A were given ligustrazine combined with cephalosporin antibiotics. The improvement of cough and other symptoms and the antipyretic time and other indexes were compared in the two groups. Results The time of disappearance of the positive signs of the lung, the antipyretic time and the time of cough disappearance in group A were significantly shorter than those in group B(P<0.05). The total effective rate in group A was 94.44％, which is significantly higher than the 72.22％ in group B(P<0.05). Conclusion Ligustrazine combined with cephalosporins on the treatment of the elderly patients with acute attack of chronic bronchitis can significantly improve the curative effect, which is worthy of clinical application.

Objective To explore the detection significance of brain natriuretic peptide in peripheral blood of patients with coronary heart disease(CHD).Methods 260 patients with CHD were selected and divided into 95 cases of stable angina pectoris(SAP) group,85 cases of unstable angina pectoris(UAP) group and 80 cases of acute myocardial infarction(AMI) group according to angiographic and clinical diagnosis.Another 80 cases of healthy people which were taken medical examination were selected as the healthy control group.The peripheral blood plasma brain natriuretic peptide concentration of the study objects were detected,and the severity of coronary lesions was quantified by the coronary Gensini score.Results The peripheral blood brain natriuretic peptide average concentration of UAP group and AMI group was (498.00 ± 87.00) ng/L and (936.00 ± 102.25) ng/L,respectively,which were significantly higher than that of the SAP group [(213.46 ± 98.35) ng/L] and the control group [(97.63 ±20.07) ng/L] (t =8.78,12.01,6.26,2.38,all P ＜ 0.05).The peripheral blood brain natriuretic peptide concentration of patients with Gensini score≥20 points was significantly higher than that of the patients with Gensini score ＜20points(P ＜ 0.05).Conclusion The peripheral blood brain natriuretic peptide concentration of patients with CHD is correlated with coronary plaque stabilization and lesion severity.

Objective:To establish a method for the determination of antioxygen 1178 in 5-layer co-extrusion bags used for infu-sion. Methods:A Uitimat XB-C8 (250 mm × 4. 6 mm,5μm) column was used,the mobile phase was methanol-water with gradient elu-tion, the flow rate was 1. 0 ml·min-1 ,the detection wavelength was 223 nm,the column temperature was 35℃ and the injection vol-ume was 20 μl. Results:The concentration of antioxygen 1178 had the linear relationship within the range of 1. 64-205. 10 μg·ml-1 (r=0. 999 9),and the average recovery was 92. 05% (RSD=1. 94%, n=9). Conclusion:The method is accurate,stable and spe-cific,and can be used to determine antioxygen 1178 in 5-layer co-extrusion bags used for infusion.

Objective:To establish a method for the determination of Si migration in penehyclidine hydrochloride injection packed with low borosilicate glass ampoule. Methods: Si migration was determined by graphite furnace-atomic absorption spectrometry. Re-sults:The linear concentration range was 0-85 ng·ml-1(r=0. 995 6). The average recovery was 95. 85% and RSD was 4. 60% (n=9). Conclusion:The method is sensitive, rapid and accurate with good repeatability, which can be used to determine Si migration in penehyclidine hydrochloride injection packed with low borosilicate glass ampoule.

Objective To investigate the incidence of insomnia in patients with nasopharyngeal carcinoma during radiotherapy and analyze the influencing factors.Method A sleep quality questionnaire for patients with nasopharyngeal carcinoma during radiotherapy was designed and used for the investigation of the incidence of insomnia as well as the influencing factors related to insomnia.Results The incidence of insomnia was 42.2%.Univariate analysis revealed that radioactive xerostomia,nausea,hospital environmental noise,radioactive oral mucositis,worry about job,worry about prognosis,burp,vomiting,worry about family influence were related to insomnia in nasopharyngeal carcinoma patients during radiotherapy;multivariate analysis showed that radioactive oral mucositis,environmental noise and job stress were the independent factors leading to insomnia(All P<0.05).Conclusions There is a high incidence of insomnia in patients with nasopharyngeal carcinoma during radiotherapy.Radioactive oral mucositis,environmental noise and job stress might be the independent factors relative with insomnia during radiotherapy.

The lacquer figure with meridian-points marked of the western Han dynasty, unearthed in Tianhui town, Jinniu district, Chengdu in 2012, has been the earliest and the most complete human figure of meridian-acupoints in China so far. There were over ten courses of meridians, and over 100 visible acupoints as well as multiple intaglio inscriptions. All of them are valuable in academic study. The writers introduced the lacquer figure un- earthed in Laoguanshan in terms of the briefs and characteristics of meridian and acupoint distributions, which give the references to the future studies.
Acupuncture ; history ; instrumentation ; Acupuncture Points ; China ; History, Ancient ; Human Body ; Humans ; Meridians

Objective This study aims to investigate the effects of high-fat diet rich in perilla oil on the expression of key genes that regulate hepatic VLDL synthesis in obese rats .Methods Sixty healthy male 5-week old SD rats were randomly divided into 2 groups.The rats in the normal control group (NC, n=12) were given normal diet, and the rats in the high fat group ( HF, n=48) were given a pure high fat diet in order to induce rat models of obesity .In the intervention period, the obesity model rats were randomly divided into 4 subgroups including consistent high fat group (CHF) and three intervention groups depending on perilla oil substitution rate of lard in CHF:20%PO, 50%PO and 100%PO.The serum triglyceride (TG) of the rats was measured after 4 weeks.Real-time PCR was applied to measure microsomal triglyceride transfer protein ( Mtp) and apolipoprotein B ( Apob) mRNA, and western blot assay was used for detecting the expression of MTP and APOB in the liver .Results Compared with the NC group , the CHF rats exhibited significantly high fat deposi-tion.The serum TG was markedly higher and the MTP and APOB were decreased at gene and protein levels in the CHF group compared with the NC group .After the intervention , PO remarkably reduced the level of serum TG and decreased he-patic fat deposition as it showed by pathological examination .At the gene and protein levels , MTP and APOB were upregu-lated by PO to different degrees .Conclusions All the three PO intervention can promote VLDL synthesis and secretion , and decrease the hepatic fat deposition in the obese rats .Furthermore , PO upregulates the expression of MTP at gene and protein levels in a dose-dependent manner .

Objective This study aims to investigate the effects of high-fat diet rich in perilla oil on the insulin sensitivity-related gene expression in skeletal muscle in insulin resistant rats.Methods The insulin resistant ( IR) rat models were randomly divided into 2 groups, including high fat group ( HF) and perilla oil ( PO) intervention group fed with 20%substitution of lard energy in the HF.The insulin sensitivity of rats was measured after 4 weeks.Theα-linolenic acid ( ALA) content of PO in the rat plasma were analyzed by gas chromatograph.Real-time PCR was applied to measure glucose transporter 4 ( GLUT4 ) and insulin receptor substrate-1 ( IRS-1 ) mRNA, and Western blot assay was used for detecting the expression of GLUT4 and IRS-1 in the skeletal muscle.Results At the gene and protein levels, PO remarkably reduced the level of IRS-1 and upregulated the level of GLUT4 with increasing intake of ALA and serum ALA content in IR rats.The results of hyperinsulinemic-euglycemic clamp test showed no significant difference between the two groups.Conclusions The results of our study suggest that consumption of n-3 PUFA at levels that can typically be found in the diet fed to IR rats in the form of ALA (0.556 g/d) may not improve insulin sensitivity, even though regulating the expression of GLUT4 and IRS-1 in the skeletal muscle.

Objective To explore the effect of nursing support on negative emotion and quality of life in patients with brain tumor. Methods A total of 139 cases of brain tumor in Shandong Provincial Cancer Hospital of Shandong University from September 2014 to February 2016 by convenient sampling , according to the random number table, they were divided into the control group and the intervention group. The patients in the control group were given routine discharge follow-up, while the patients in the intervention group were given emote team nursing support on the basis of the control group. We used anxiety self rating scale (SAS), depression self rating scale (SDS) and quality of life assessment scale (QLICP-BN) evaluation two groups of patients with anxiety, depression and quality of life level in the patients when they were discharged from hospital and discharged after 1 month, 3 months. Results Comparing the two groups of patients with anxiety and depression level and quality of life in the hospital, there was no statistically significant difference (t=0.187,0.174, P > 0.05);1 month after discharge, the scores of SAS and SDS respectively (62.97 ± 485), (63.83 ± 5.24) points in the intervention group, the scores of SAS and SDS respectively (64.58 ± 5.15), (65.17 ± 5.11) points in the control group, the difference is statistically significant (t=2.753, 2.321, P<0.05);3 month after discharge, the scores of SAS and SDS respectively(61.04±4.13),(62.25±3.95)points in the intervention group, the scores of SAS and SDS respectively(63.91 ± 4.73),(64.83 ± 4.29)points in the control group, the difference is statistically significant (t=4.621,5.196, P < 0.01); 1month, 3 months after discharge, the total quality of life scores respectively (65.28 ± 12.53), (68.71 ± 12.78) points in the intervention group, the total quality of life scores respectively (62.07 ± 11.27), (63.86 ± 12.13) points in the control group, the difference is statistically significant (t=2.439, 3.803, P < 0.01). The intervention effects and time effects of anxiety, depression and quality of life were statistically significant (W Mauchly's were 0.823, 0.782, 0.757, P <0.05). Conclusions Remote team nursing support can effectively reduce the negative emotions of patients with brain tumor, improve the quality of life, can be used in the department to promote the quality of clinical care.

Objective To measure the endogenous metabolites in synovial tissues of rheumatoid arthritis (RA) rabbits with cold syndrome;To investigate the specificity mechanisms of heat-reinforcing needling for RA. Methods A total of forty healthy purple blue rabbits were randomly allocated to normal group, model group, reinforcing-reducing needling group (RRN), twirling-reinforcing needling group (TRN) and heat-reinforcing needling group (HRN) 8 rabbits in each group. Arthritis rabbits cold model was made with ovalbumin and freezing. Except for normal groupand model group, RRN was given acupuncture of reinforcing-reducing needling at Zusanli (ST36), TRN was given acupuncture of twirling-reinforcing needling at Zusanli (ST36), HRN was administrated acupuncture of heat-reinforcing needling at Zusanli (ST36), once a day and retaining 30 min, a total of seven days. Fresh synovial tissues of rabbits knee joints were extracted after the intervention, then GC-Q/TOF-MS technology were employed to evaluate metabolic profiles. Results The synovial tissues metabolites of TCA cycle, carbohydrate and fatty acid metabolism in model group mainly increased compared with normal group (P<0.05); The synovial tissues metabolites of various kinds above-mentioned decreased compared with model group in all intervention of acupuncture group (P<0.05), and the metabolites of TCA cycle and carbohydrate metabolism in HRN were obviously less than that of RRN and TRN (P<0.05). Conclusion The specificity of heat-reinforcing needling for RA manifests in regulation of TCA cycle and carbohydrate metabolism.