Objective To investigate the relationships among perceived stress,mindfulness level,and sleep problems,and to indicate the mediating role of mindfulness level in the relationship between perceived stress and sleep problems.Methods For 179 undergraduate students,the Five Facet Mindfulness Questionnaire (FFMQ),Chinese Perceived Stress Scales (CPSS) and Athens Insomnia Scale (AIS) were used to measure.Results The scores of the scales were (120.43 ± 1 1.23) for FFMQ,(40.17 ± 6.86) for CPSS and (5.28 ± 4.04)for AIS.Pearson correlation analysis showed that perceived stress was negatively correlated to mindfulness level (r =-0.602,P =0.000) and positively correlated to sleep problems (r =0.459,P =0.000) and that there was significant negative correlation between mindfulness level and sleep problems (r =-0.427,P =0.000).Hierarchical linear modeling showed significant partial mediation of mindfulness level on the association between perceived stress and sleep problems.Conclusion Mindfulness level partly mediated the positive link between perceived stress and sleep problems.

Expression of apelin mRNA in the isolated mouse adipocytes was confirmed by Northern blot. The level of apelin mRNA increased during stage of differentiation of 3T3-L1 cells. Insulin up-regulated the apelin expression in 3T3-L1 adipocytes, suggesting that apelin expression in adipocyte may correlate with obesity, insulin resistance and hypertension.

Objective To test the effect of EPO on anemia of preterm infant. Methods Thirty preterm infants less than 35 weeks of gestational age from three different hospitals were devided ramdomly into EPO group of 16 cases and control group of 14 cases. The infants in EPO group were received rh EPO 400 IU/(kg?d) subcutaneously twice a week for 4 weeks from 7 days old, together with oral supplementation of ferrous sulfate at a dossage of 3~5 mg/(kg?d) in both groups. Hemoglobin(Hb) and reticulocytes(Ret) were measured at 42 days of age. Results The hemoglobin was higherin the EPO group[(999?29)g/L vs (905?25)]g/L, P 0.05). Conclusion The is lower EPO level in prematures and efficiency of rh EPO in the treatment of anemia of preterm infants. There is no observed substantial adverse effects.

Objective:To investigate the effectiveness of mindfulness training improving negative emotions, furthering to explore the mediating effect of the improvement of mindfulness levels for the training effects.Methods:Randomized control trail was used in this research.Ninety participants who suffered negative e-motions and resolved to alleviate stress by mindfulness training were recruited by lecture.They were randomized in-to mindfulness training group or waitlist control group,and 79 of them completed the intervention (38 in mindful-ness training group and 41 in waitlist control group).Participants in mindfulness training group received an 8-week mindfulness training,while participants in waitlist control group received no intervention.Participants were assessed with the Five Facet Mindfulness Questionnaire (FFMQ,measuring mindfulness level)and Brief Profile of Mood State (POMS,measuring emotion)before and after intervention.Results:After 8-week intervention,participants in mindfulness training group got significantly higher total scores of FFMQ compared with baseline [(125.9 ±11.9) vs.(121.2 ±12.5),P<0.01].Participants in mindfulness training group also got significantly higher total scores of POMS,and the intensity-anxiety,depression-frustration,and fatigue scores of POMS compared with baseline (Ps<0.01).There were no significant differences between posttest and baseline on any scores in waitlist control group (Ps>0.05 ).The improvement of total scores of FFMQ mediated the reduction of total scores of POMS (95%CI=-6.24--0.73),and the intensity-anxiety (95%CI=-1.65 ~-0.12),depression-frustration (95%CI=-1.63--0.14),and fatigue scores (95%CI=-1.72--0.20)of POMS caused by mindfulness training.Conclusion:Mindfulness training could improve participants'negative emotions,and the enhanced mindfulness levels caused by mindfulness training will play a very important role for the improvement of negative emotions.

Objective To evaluate the effect of supplementation with human milk fortifier (HMF) produced by human milk protein in low birth weight infants.Methods Eighty seven low birth weight infants undergoing neonatal intensive care were divided into 4 groups randomly,human milk +HMF 9307(enriched protein 6 g/L)and human milk;formula +HMF 9308(enriched SIgA 500 mg/L)and formula.Results The weight gain was faster in human milk+HMF 9307 group than that of human milk group(13.8 g?kg~(-1)?d~(-1).vs.9.4 g?kg~(-1)?d~(-1),P

0.05). Conclusion: RVPI and RSVPI of can be used as a measure to assess VPF and speech articulation of CP patients after operation.

BACKGROUND: Increase of fibrinogen is an independent risk factor for cardio-cerebrovascular disease and peripheral angiopathy. Fibrinogen and its degradation product exist in carotid atheromatous plaque and it can stimulate proliferation and migration of smooth muscle cells so as to play a key role in early atherosclerosis.OBJECTIVE: To analyze the relationship between plasma fibrinogen and carotid atheromatous plaque.DESIGN: Descriptive observation.SETTING: Department of Neurology, Xinhua Hospital, Medical Collegeof Shanghai Jiaotong University.PARTICIPANTS: A total of 81 patients with ischemic stroke were selected from Neurological Department of Xinhua Hospital affiliated to Medical College of Shanghai Jiaotong University from September to December 2001.There were 53 males and 28 females aged 41-85 years with the mean age of (65±11) years.METHODS: On the basis of carotid ultrasonography, 81 patients with ischemic stroke were divided into present plaque group (n=45) and absent plaque group (n=36) to measure plasma fibrinogen, serum total cholesterol (TC), low density lipoprotein cholesterol (LDL-C) and other risk factors of atherosclerosis so as to observe the correlation between risk indexes and carotid atheromatous plaque.MAIN OUTCOME MEASURES: Risk indexes of carotid atherosclerosis and carotid atheromatous plaque.RESULTS: All 81 patients were involved in the final analysis. ① Values of fibrinogen, TC and LDL-C in present plaque group were (4.38±1.33) g/L,(5.19±1.27) mmol/L and (3.15±0.37) mmol/L, respectively, which were higher than those in absent plaque group [(3.20±1.30) g/L, (4.56±1.30) mmol/L,(2.49±0.92) mmol/L, P ＜ 0.05]. ② Ratios of carotid atheromatous plaque were 11% (5 cases), 16% (7 cases) and 73% (33 cases) in fibrinogen groups at Iow, moderate and high dosages, respectively. There was significant difference (P =0.002). ③ Multiple factor analyses of carotid atherosclerosis showed that the risk factors which promoted the formation of carotid atheromatous plaque were diabetes mellitus, low density lipoprotein (LDL), fibrinogen and age growing.CONCLUSION: Fibrinogen plays an active role in development and progression of carotid atheromatous plaque.

BACKGROUND: According to up-to-date development, the best timing to repair cleft palateis at 9-12 months old after birth when the articulation does not begin to develop.However, there are many over-eight-year-old children who still suffer from unrepaired cleft palate. Therefore, it is veryimportant to adopt proper surgery for these patients to improve palate form and articulatory function. OBJECTIVE: To study the characteristics of the cleft palate in patients of over 8 years old and the effects of palate repair and posterior pharyngeal flap transplantation (PPFT) on articulation. DESIGN:A controlled study with children patients as subjects. SETTING: Department of Maxillofacial Surgery, Medical College of Stomatology, Fourth Military Medical University of Chinese PLA PARTICIPANTS: Twenty-four children patients of cleft palate who were treated in the Department of Maxillofacial Surgery ,Medical College of Stomatology, Fourth Military Medical University of Chinese PLA from January 2000 through December 2003 were enrolled in the study. Fourteen were male and ten female. Their ages varied from 8 to 20 years old (mean 13.8 years). Three cases were bilateral complete cleft palate and twelve cases were unilateral complete cleft palate. Nine were incomplete cleft palate. METHODS :Two flap palatoplasty and upper pedicle PPFT were conducted in all the patients. In thirty days after surgical treatment, the patients were asked to repeat the words of a doctor in the phonetic lab with his or her lips 10 cm away from microphone. The words of the patients were recorded and evaluated subjectively by 5 doctors specialized in pathologic phones. MAIN OUTCOME MEASURES :The palatopharynx was observed with nasopharyngoscope before and after operation. And the improvement of nasal gas leakage and enhanced rhinophonia were also evaluated. RESULTS: The repaired cleft palate was healed in the first intention in all the patients. The soft palate retreated satisfactorily and the palatopharynx was apparently closed, which created good preconditions for articulation. The patients' articulation was also improved in different degrees. All the 24 patients had the fourth grade of enhanced rhinophonia and nasal gas leakage before repairing. After palate repair and PPFT, the enhanced rhinophonia and nasal gas leakage of grade 1 was in 3 patients, grade 2 in seven, grade 3 in six and grade 4 in eight patients. CONCLUSION: The combination of palate repair and pharyngoplasty is able to improve the form of soft palate and the articulation of over-eightyear-old patients with cleft palate.

Objective To investigate the effects of angiotensin1-7 (Ang1-7) on renal tubulointerstitial fibrosis of diabetic rats.Methods Thirty-two male Wistar rats were randomly divided into four groups: normal control group,diabetic group,telmisartan group,Ang1-7-treated group.For 9 weeks after diabetes mellitus model established,24 h proteinuria,urine NAG/Cr,glucose,insulin,TG,TC,BUN,Scr,Na+ and K+ were assessed.Renal pathological changes were evaluated by PAS staining; Expression of TGF-β1,PPARγ and α-SMA mRNA was deteeted by real-time PCR; Protein levels of PPARγ,α-SMA and TGF-β1 were detected by Western blotting.Results (1)At the end of the ninth week,the blood pressure,proteinuria,renal weight/body weight in group DM were significantly higher than those in group NC (P＜0.05).(2)Renal interstitial fibrosis in group DM was obviously severe as compared to group NC (P＜0.05),but was improved in group TM and group T(P＜0.05).(3)TGF-β1 and α-SMA mRNA in group DM were significantly increased,and PPARγ mRNA was significantly decreased.Compared with group DM,TGF-β1 and α-SMA mRNA were significantly decreased,and PPARγ mRNA was significantly increased in group TM and group T,especially in group T.(4)TGF-β1 and α-SMA in group DM were significantly increased,and PPARγ decreased significantly.Compared with group DM,TGF-β1 and α-SMA decreased significantly,PPARγ increased significantly in group TM and group T,especially in group T.Conclusion Ang1-7 inhibits high glucose-induced α-SMA expression in vivo through up-regulating the PPAR expression and may inhibit renal tubulointerstitial fibrosis of diabetic rats.

Objective To report filaminopathy with novel insertion mutation in a Chinese family.Methods Total 19 patients from successive 5 generations involved in an autosomal dominant family. The detailed clinical manifestations had been described (Chinese Journal of Neurology, 2008, 41:751-755).The filamin C gene sequencing was performed in 3 patients, 5 family members without symptoms and 50 normal persons. The amplified fragments of the exon 18 in filamin C gene were cloned into pBluesripts vectors, then sequenced and identified with capillary electrophoresis. Results 18-nucleotide deletion and 6-nucleotide insertion were identified in the exon 18 of filamin C gene. The mutation caused the disturbance of the seventh immunoglobulin-like domain in filamin C, leading to the instability of dimmers of filamin C.Another 2 patients in the family had same mutation while 5 family members without symptoms and 50 normal controls were normal. Conclusion The novel nucleotide deletion-insertion in exon 18 of filamin C gene causes filaminopathy. This disease can appear in non-Nordic race.