BACKGROUND:Traditional cel transplantation tracer methods require histological analysis and identification in vitro, which limits the clinical application of stem cel transplantation. So it is urgent to establish an in vivo noninvasive and repeatable tracer method. OBJECTIVE:To observe the effect of SPIO and DAPI double labeling on survival and proliferation of bone marrow mesenchymal stem cel s from macaques. METHODS:Bone marrow mesenchymal stem cel s were derived from bone marrow aspirates of healthy macaques using whole bone marrow adherence method. Then, the cel s were identified using flow cytometry detection. Bone marrow mesenchymal stem cel s were labeled using SPIO and DAPI. Fluorescent microscope was used to detect DAPI positive rate, and Prussian blue staining and transmission electron microscope were employed to measure SPIO positive rate. MTT assay was used to detect cel viability and proliferation. RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cel s were successful y isolated from healthy macaques using the whole bone marrow adherence method, and the cel purity was up to 95.1%. SPIO and DAPI were both successful to label the bone marrow mesenchymal stem cel s with a positive rate of 95%-98%, but had no influence on cel viability and proliferation.

Objective To compare the effects of phenylephrine and norepinephrine in patients with restrictive transfusion.Methods 65 patients undergoing laparoscopic resection of rectal cancer were selected.According to the random number table method,the patients were randomly divided into 32 cases of observation group (phenylephrine composite restriction infusion) and 33 cases of control group(norepinephrine combined restrictive transfusion).The operation and hemodynamics of the two groups at different time points were compared.At T0,T1,T2,the central venous blood and arterial blood were collected,the central venous blood saturation (ScvO2),arterial blood lactate (Lac),central venous oxygen partial pressure (PcvO2),PaO2,Hb concentrations,venous oxygen content (CcvO2),arterial oxygen content(CaO2),arteriovenous oxygen content difference (Ca-cvO2) and oxygen uptake rate (ERO2) were compared between the two groups.Results The ASA levels (Ⅰ / Ⅱ grade) of the observation group and the control group were 11 cases/21 cases and 13 cases/20 cases,respectively,the difference was not statistically significant(x2 =5.298,P =0.052).The BMI of the observation group and the control group were (20.61 ± 1.65) kg/m2,(21.57 ± 1.42) kg/m2,respectively,the difference was not statistically significant (t =0.652,P =1.980).The fluid replacement of the observation group and the control group were (1 162 ± 113) mL,(1171 ± 104) mL,respectively,the difference was not statistically significant (t =1.734,P =0.065).The blood loss of the observation group and the control group were (276 ± 41) mL,(294 ± 42) mL,respectively,the difference was not statistically significant (t =0.083,P =1.023).The urine volume of the observation group and the control group were (524 ± 113) mL,(532 ± 109) mL,respectively,the difference was not statistically significant(t =1.834,P =0.053).The operation time of the observation group and the control group were (192 ± 24) min,(197 ± 21) min,respectively,the difference was not statistically significant(t =1.367,P =0.073).The MAP at T1-T2 of the two groups increased,the CVP of the two groups was significantly higher at T1,the HR of the two groups was significantly higher at T2.The MAP,CVP,HR between the two groups had no significant differences (all P ＞ 0.05).The levels of ScvO2,PcvO2 and PaO2 at T1 were significantly higher than those at T0,on the contrary,the levels of ScvO2,PcvO2 and PaO2 at T2 were significantly lower than those at T0,and the Hb in the observation group was significantly lower at T1-T2,the Hb in the control group was significantly lower at T,-T2,which in the observation group was lower than the control group,the differences were statistically significant(all P ＜ 0.05).There was no statistically significant difference in Lac between the two groups (P ＞ 0.05).There were statistically significant differences in CcvO2,CaO2,Ca-cvO2 and ERO2 between the two groups at different time points (all P ＜ 0.05).Conclusion Phenylephrine is more effective than norepinephrine in limiting infusion in elderly patients,and it has more obvious effects on tissue oxygen metabolism.

Objective To retrospectively evaluate the diagnostic accuracy of endoscopic ultrasonog raphy ( EUS) in pancreatic cancer, analyze the value of EUS in measurement of tumor diameters, TNM staging, and investigate the effectiveness of endoscopic ultrasonography-guided fine needle aspiration ( EUS-FNA). Methods All the data adopted from the patients underwent EUS in PUMC Hospital between 1997 and 2003. Eighty-eight patients with a final diagnosis of pancreatic cancer (56 is proven by surgical pathology, 10 by surgical findings and 22 by clinical diagnosis) and 47 patients with a final diagnosis of benign pancreatic disease were included and analyzed. Results The sensitivity, specificity, accuracy, positive and negative predictive values of EUS in the diagnosis of pancreatic cancer were 95. 5% , 59. 6% , 83. 0% , 81. 6% and 87. 5% respectively. Tumor diameters measured by EUS (2. 8?1. 0)cm were obviously smaller than those by surgical findings (6.5 ? 2. 9) cm. For TNM stage, compared with surgical findings, the accordance rates of EUS in T and N staging were 43. 1% and 61. 1% respectively. The accuracy of EUS-FNA in the diagnosis of pancreatic cancer was 76. 5% ( 13/17). Conclusions The sensitivity of EUS in pancreatic cancer is highest among all imaging procedures. Tumor diameters measured through EUS are smaller than those by surgery.

BACKGROUND: Micro-implant screws as an absolute anchorage, instead of traditional anchorage that requires patient's high matching and affects beauty, has been paid more attention.OBJECTIVE: To compare the changes in 3 different places following implantation of micro-implant screws. DESIGN, TIME AND SETTING: The controlled study was performed at the Hospital of Stomatology Affiliated to Foshan College of Sciences and Technology from January 2004 to December 2007.PARTICIPANTS: A total of 45 patients who demand powerful anchorage during the orthodontic treatment were assigned to 3 groups: free gingiva group, mucogingival junction group, and mucosa group.METHODS: By using incomplete flap micro-implant screw implantation, micro-implant screws were implanted in the free gingiva, mucogingival junction and mucosa, with 30 screws in each area.MAIN OUTCOME MEASURES: Loosening and defluvium of micro-implant screws were measured. Pain duration and degree were determined in patients undergoing implantation of micro-implant screws 7 days following surgery. Soft tissue surrounding the micro-implant screws and bleeding rate during probing were detected 56 days following surgery.RESULTS: The number of screw loosening was greatest in the free gingiva group (9 screws), while lowest in the mucogingival junction group (2 screws). Patients suffered from severe flare and pain in the mucosa, and the reaction was mild in the free gingiva following implantation. The reaction was not obvious, but severe pain was found in the mucogingival junction following implantation compared with the free gingiva. Soft tissue surrounding the micro-implant screws and bleeding rate during probing were greater in the mucosa group than in the free gingiva group and mucogingival junction group (P<0.05).CONCLUSION: The incomplete flap micro-implant screws in the mucogingival junction contribute to the increase in success implantation rate and the decrease in inflammatory degree, and prevent the occurrence of potential risk.

Objective To investigate the correlation between acute physiology and chronic health evaluation Ⅱ (APACHEⅡ) scores and delirium probability of senile severe pneumonia patients undergoing invasive mechanical ventilation (MV).Methods A retrospective study was conducted. Eighty-nine senile severe pneumonia patients undergoing invasive MV admitted to intensive care unit (ICU) of Hunan Provincial People's Hospital from January 2015 to March 2017 were enrolled. APACHE Ⅱ scores were collected 24 hours before invasive MV. Consciousness assessment method-ICU (CAM-ICU) was used to diagnose delirium, and the patients were divided into delirium group and non-delirium group. The first delirium occurrence time, duration of MV and the length of ICU stay were recorded. The patients were divided into ≤15, 16-20, 21-25, 26-30, 31-35, 36-40 groups according to APACHEⅡ score, and the incidence of delirium in all groups were observed. The linear regression and Pearson correlation were used to analyze the correlation between APACHE Ⅱ scores and delirium probability. Receiver operating characteristic (ROC) curve was plotted to analyze the predictive effect of APACHEⅡ score on delirium.Results Eighty-nine patients were enrolled in the final analysis, of which 35 had delirium, and 54 had no delirium, with delirium incidence of 39.33%, and the first delirium occurrence time of (1.85±1.30) days. The duration of MV and the length of ICU stay of delirium group were significantly higher than those of non-delirium group [duration of MV (days): 9.43±4.77 vs. 6.08±3.30, length of ICU stay (days): 14.60±6.59 vs. 9.69±4.61, bothP < 0.01]. The APACHE Ⅱ score in delirium group was significantly higher than that in non-delirium group (29.89±5.45 vs. 21.48±4.76,P < 0.01). With the increase in APACHE Ⅱ scores, the delirium incidence was gradually increased. Correlation analysis showed that there was a negative correlation between APACHE Ⅱ scores and first delirium occurrence time (r = -0.411,P = 0.014), and a significant linear positive correlation between APACHE Ⅱ scores and delirium incidence in all patients was found (r = 0.982, P = 0.000), which indicated the higher APACHE Ⅱ scores, the higher delirium incidences and the earlier first delirium occurrence time was. ROC curve analysis showed that the area under ROC curve (AUC) of APACHE Ⅱ scores on predicting delirium occurrence was 0.877, when the cut-off value of APACHE Ⅱ score was over 27, the sensitivity was 92.59%, the specificity was 71.43%, the positive predictive value was 83.33%, and the negative predictive value was 86.21%.Conclusions With the increase in APACHE Ⅱ score, the incidence of delirium was increased gradually in senile severe pneumonia patients receiving invasive MV. APACHE Ⅱ score played an important clinical value in evaluating the delirium probability of these patients

Objective To analyze the clinical characteristic and provide therapeutic techniques of maxillofacial trauma in the elderly. Methods Geriatric maxillaofacial trauma patients admitted to our hospital from January 1992 to December 2002 was retrospectively reviewed. Data were collected according to etiology, location, comorbidities, associated injuries, therapeutic methods and the outcome. The patients were compared with a control group consisting of 200 young and adult patients admitted at the same period. Results For the 126 geriatric trauma patients, the major causes were traffic accidents(61.1%) and violence assault(25.4%). A large proportion of facial injuries occurred on the lower part of the face. 69.1% of the patients presented with preexisting diseases. Most of the fractures were not treated(35.7%). The length of hospitalization and ICU stay were relatively longer when compared with the control group(P

ObjectiveTo explore the diagnostic value of 16-slices CT in urinary calculus. Methods25 cases of suspected urinary calculus were examined by 16-slices CT and some of these images were reconstructed with MPR. ResultsIn the 25 casces CT found 21cases of urinary calculus,among them kidney calculus 22 cases,urinary tract calculus 13 cases.In 5 of 21 cases,urinary calculus were not found with US.In all 6 cases urinary calculus were not detected by KUB and/or IVP. Conclusion16-slices CT was a valuable method in diagnosing urinary calculus.

0.05).The patients with positive P gp and LRP expression responded more poorly to chemotherapy than those with negative P gp and LRP expression ( P

Objective: To establish the quality standards for Jifukang Oral Solution. Methods: Fructus Corni, Fructus Ligustri Lucidi, Herba Epimedii were identified by TLC, and the content of astragalin A was determined by TLC scanning. Results: The Fructus Corni, Fructus ligustri licidi, Herba Epimedii could be identified by TLC. Astragalin A showed a good linear relationship at a range of 1.065～5.325?g,r=0.9991. The average recovery was 95.1%, and RSD was 2.0%. Conclusion: The methods are accurate and can be used for the quality control of Jifukang Oral Solution.

Objective To investigate the effect of human umbilical cord blood stem cells on flash visual evoked potentials (F-VEP) of the traumatic optic neuropathy rats.Methods Forty-eight Sprague-Dawley rats were randomly divided into an injury group (Group A) and 3 treatment groups (Groups B,C,and D).A traumatic optic neuropathy model was built in Group A,and the rats in Groups B,C,and D were injected with the neurotrophic factor,human umbilical cord blood stem cells,and the mixture of the neurotrophic factor and human umbilical cord blood stem cells,respectively.F-VEP was recorded in both eyes of rats at the 1st h,1st week,2nd week,3rd week,and 4th week after the optic nerve injury.Results At all time points,there were significant difference in the wave latency and amplitude between Group A and normal control eyes (P＜0.01).The differences of the wave latency and amplitude between Group A and Groups B,C,and D were statistically significant at various time points after the injury except for the wave latency at the 1st h post-operation (P＞0.05).The amplitude in Group D was higher while the latency was shorter than those of Group B at all time points since the 1st week (P＜0.05).The comparisons at the same point in the remaining treatment groups were not significantly different (P＞0.05).Conclusion The mixture of human umbilical cord blood stem cells and neurotrophic factor has a promotion effect for the recovery of F-VEP of optic nerve in traumatic optic neuropathy in rats to some degrees.