Objective To investigate the changes of relevant characteristics in the Beagle model of type 2 diabetes mellitus(T2DM) induced by high fat diet and low dose streptozotocin (STZ) injection. Methods Thirty male Beagles were randomly divided into three groups:(1) Control group (n=10), fed with a standard chow. (2) High fat diet group (n=10), fed with high fat diet. (3) Model group (n=10), fed with high fat diet for two months and then given STZ in?jection. Lee index, fasting blood glucose, serum insulin levels, urine glucose and blood biochemical indexes were regularly detected. Oral glucose tolerance test ( OGTT) and histopathological examination were performed. Results After treatment for two months, the insulin resistance and dyslipidemia appeared and Lee index significantly increased in the high fat diet group and model group (P<0?01). In the diabetic beagles, fasting blood glucose levels were prominently increased (P<0?01), as compared with the control group and high fat diet group, and held on a high blood glucose level for three months. Among the three groups, the dogs with OGTT values>11?1 mmol/ L and were not restored at three hours, showed some pancreatic histological damages and diseases. Conclusions A beagle model of type 2 diabetes mellitus is construc?ted, exhibiting some characteristics of human type 2 diabetes mellitus such as hyperglycemia,hyperinsulinism,dyslipidemia and other typical features.

Objective To establish the effect of curcumin on PTX-resistant esophageal cancer cell line EC9706/PTX and to investigate the mechanism of curcumin on the epithelial stromalization (EMT) of EC9706/PTX cells.Methods EC9706/PTX cells were established by medium concentration intermittent method.The drug resistance index and cross resistance were measured by MTT assay.The inhibitory effects of different concentrations of curcumin on EC9706/PTX cell proliferation were detected.The effects of curcumin on the morphological changes,migration and invasion of EC9706/PTX cells were examined by cytostatic staining,scratching and transwell invasion assay.The effects of curcumin on the expression of E-cadherin,N-cadherin,vimentin and fibronectin in EC9706/PTX cells at mRNA and protein levels were detected by fluorescence quantitative PCR and Western blot.The effect of curcumin on the expressions of NF-κB p65 and Snail in EC9706/PTX cells were detected by Western blot.Results The drug resistance index of EC9706/PTX was 28.4,which was cross-resistant to cisplatin and doxorubicin.Curcumin could inhibit the proliferation of EC9706/PTX cells.The migration and invasion of EC9706/PTX cells were significantly decreased under the action of curcumin at 20 μmol/L concentration.Fluorescence quantitative PCR and Western blot analysis showed that the expression of Ecadherin was down-regulated and the expression of N-cadherin was up-regulated,and curcumin reversed this phenomenon.Western blot analysis showed that the expression of NF-κB p65 and Snail protein was enhanced after PTX-resistant generated in EC cell,but curcumin reversed this phenomenon.Conclusion Curcumin can inhibit the proliferation,migration and invasion of EC9706/PTX cells.The mechanism maybe that curcumin inhibits the NF-κB-Snail pathway.